##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527511_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739689 Sequences flagged as poor quality 0 Sequence length 52 %GC 58 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.839405479870596 31.0 31.0 33.0 30.0 33.0 2 30.95458226362701 31.0 31.0 34.0 28.0 34.0 3 31.873538743985648 34.0 31.0 34.0 30.0 34.0 4 34.34319153049457 35.0 35.0 35.0 32.0 37.0 5 35.24457711281363 35.0 35.0 37.0 33.0 37.0 6 34.52572635256168 35.0 35.0 36.0 32.0 37.0 7 35.903969100527384 37.0 35.0 37.0 35.0 37.0 8 36.4405081054335 37.0 37.0 37.0 35.0 37.0 9 38.611312321800106 39.0 39.0 39.0 38.0 39.0 10 37.433190165055855 39.0 37.0 39.0 34.0 39.0 11 36.91919306627515 39.0 37.0 39.0 33.0 39.0 12 36.82766676265295 39.0 35.0 39.0 33.0 39.0 13 36.64936750445119 39.0 35.0 39.0 33.0 39.0 14 37.52985105902616 39.0 37.0 40.0 33.0 40.0 15 37.69824209904433 40.0 37.0 40.0 33.0 40.0 16 37.70815302106696 40.0 37.0 40.0 33.0 40.0 17 37.78341438090873 40.0 37.0 40.0 33.0 40.0 18 37.68409155739777 40.0 37.0 40.0 33.0 40.0 19 37.65449263136264 40.0 37.0 40.0 33.0 40.0 20 37.48441034002128 39.0 37.0 40.0 33.0 40.0 21 37.54680818560233 39.0 37.0 40.0 33.0 40.0 22 37.56260266138877 39.0 37.0 40.0 33.0 40.0 23 37.54526429350714 39.0 37.0 40.0 33.0 40.0 24 37.51405523132019 39.0 36.0 40.0 33.0 40.0 25 37.421379796103494 39.0 36.0 40.0 33.0 40.0 26 37.358867037363 39.0 36.0 40.0 33.0 40.0 27 37.23515152989973 39.0 36.0 40.0 33.0 40.0 28 37.10388960765943 39.0 36.0 40.0 33.0 40.0 29 37.09127079083236 39.0 35.0 40.0 32.0 40.0 30 36.93545530621653 39.0 35.0 40.0 32.0 40.0 31 36.81234545869953 39.0 35.0 40.0 32.0 40.0 32 36.68103892311498 39.0 35.0 40.0 31.0 40.0 33 36.48376141865027 38.0 35.0 40.0 31.0 40.0 34 36.1498007946583 38.0 35.0 40.0 30.0 40.0 35 36.084581493032886 38.0 35.0 40.0 30.0 40.0 36 36.05337919044355 38.0 35.0 40.0 30.0 40.0 37 35.940815667124966 38.0 35.0 40.0 30.0 40.0 38 35.870611838218494 38.0 34.0 40.0 30.0 40.0 39 35.6830086698599 38.0 34.0 40.0 30.0 40.0 40 35.81398398516133 38.0 35.0 40.0 30.0 40.0 41 35.845101116820715 38.0 35.0 40.0 31.0 40.0 42 35.806876944229266 37.0 35.0 40.0 31.0 40.0 43 35.72533591820346 37.0 35.0 40.0 30.0 40.0 44 35.551268168108486 37.0 34.0 40.0 30.0 40.0 45 35.46062196409572 37.0 34.0 40.0 30.0 40.0 46 35.15494484844306 36.0 34.0 40.0 30.0 40.0 47 35.07003348704659 36.0 34.0 40.0 29.0 40.0 48 34.86528257145908 35.0 34.0 39.0 29.0 40.0 49 34.64234563444907 35.0 34.0 39.0 29.0 40.0 50 34.45142079982263 35.0 33.0 39.0 28.0 40.0 51 34.272640258270705 35.0 33.0 39.0 28.0 40.0 52 33.69218549958158 35.0 33.0 38.0 26.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 6.0 13 11.0 14 23.0 15 34.0 16 58.0 17 102.0 18 163.0 19 298.0 20 481.0 21 698.0 22 999.0 23 1518.0 24 2254.0 25 3131.0 26 4401.0 27 5959.0 28 8053.0 29 10678.0 30 13353.0 31 17991.0 32 24678.0 33 35803.0 34 61037.0 35 67874.0 36 107395.0 37 155824.0 38 196235.0 39 20627.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.72258611389381 0.13370484081823578 0.1174818065430201 0.026227238744931995 8 99.44706491511973 0.27633235048784016 0.22942074304200819 0.04718199135041889 9 98.67579482728553 0.8050680759075773 0.2463197370786912 0.2728173597282101 10 65.03882036910107 22.34074050039949 4.551237073959461 8.069202056539979 11 35.43651453516275 28.298514646020152 19.20347605547737 17.061494763339727 12 36.87049557313952 15.398633750130122 25.258318022844733 22.472552653885618 13 24.446490349322485 15.393361263990677 31.2759822033314 28.884166183355436 14 31.07143677951139 16.179637658529465 28.540778624530038 24.20814693742911 15 26.25792731810261 14.00872528860102 25.08067579753113 34.65267159576525 16 30.99410698279953 20.540389271707436 27.129104258681693 21.33639948681135 17 38.63677842985363 14.811089525462728 21.38479820573241 25.167333838951233 18 29.019898903458074 15.685646264849145 30.19944868721855 25.09500614447423 19 37.06976851082009 16.982407471248052 20.88377683053283 25.06404718739903 20 27.934577910446144 17.22277876242583 25.214786214206242 29.62785711292178 21 32.23260045775995 16.76488362000787 28.880786384548102 22.121729537684082 22 31.604228263499927 15.5562675665043 24.790553867909352 28.048950302086418 23 33.65563094760095 16.929547417901308 27.937011365587427 21.477810268910314 24 38.890533724308455 16.516536003644774 21.579339425082704 23.013590846964064 25 29.96854083270131 16.223710234977133 28.193064923231248 25.614684009090304 26 35.25211271223447 16.45231982630538 22.72752467591109 25.56804278554906 27 30.842691996230847 15.2998084330036 26.18032713748616 27.67717243327939 28 37.74708019181034 18.698939689518163 18.69136894018973 24.862611178481767 29 37.34272106250059 19.436546981231302 19.132635472475595 24.088096483792512 30 35.490456124127846 19.783584722768623 20.4211499697846 24.304809183318934 31 36.66432784589199 20.698834239795374 18.852382555371243 23.784455358941393 32 36.7780242777708 20.28582282553884 20.236477762951726 22.69967513373864 33 34.23600999879679 20.492531320595546 21.358030199178305 23.913428481429357 34 30.413322355746807 22.6508708389607 24.25032682654467 22.685479978747825 35 29.931633429725196 25.999981073126676 21.983833746344747 22.084551750803378 36 30.413457547699103 25.85032358193781 20.709244020121968 23.026974850241114 37 31.550557058439427 25.652672947684767 19.28540237856721 23.511367615308597 38 31.08928211721413 25.80679177329932 19.637036646482507 23.466889463004044 39 31.774299739485105 23.580721086835144 20.82591467495123 23.81906449872852 40 30.247036254425847 23.3417017151803 19.209694885282868 27.20156714511098 41 27.147084788336716 24.425805980621583 20.6031183375716 27.82399089347009 42 26.67634641045088 21.47456566205527 22.52919808189658 29.31988984559727 43 27.324051053888866 20.71384054649995 24.50719153590225 27.454916863708938 44 27.824666853231562 21.295706709171018 23.54313772409756 27.33648871349986 45 29.29068838390188 21.011803609354743 22.536498447320426 27.161009559422943 46 28.174543625767047 23.602757375058978 22.24894516479223 25.973753834381746 47 27.74584994504447 22.30248117790044 22.45186828518472 27.499800591870365 48 28.633520303803355 23.436065697881137 23.349002080604144 24.581411917711364 49 30.441847857680727 22.19081262530604 21.59285862031205 25.774480896701178 50 26.605776211353692 22.687237474127638 22.8328392067477 27.87414710777097 51 27.32229355850905 24.108375276636533 22.251784195790393 26.317546969064026 52 27.601600131947347 23.58301935002413 22.123892608920777 26.691487909107746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.0 8 1.0 9 12.5 10 24.0 11 65.5 12 107.0 13 197.5 14 464.5 15 641.0 16 591.5 17 542.0 18 563.0 19 584.0 20 784.0 21 984.0 22 944.5 23 905.0 24 954.5 25 1004.0 26 1475.5 27 1947.0 28 2010.0 29 2073.0 30 2671.5 31 3270.0 32 4607.5 33 5945.0 34 6366.0 35 6787.0 36 8060.0 37 9333.0 38 9493.5 39 10460.5 40 11267.0 41 10973.0 42 10679.0 43 11995.5 44 13312.0 45 14358.0 46 15404.0 47 16958.5 48 18513.0 49 18843.0 50 19173.0 51 23232.5 52 27292.0 53 30293.0 54 33294.0 55 39141.0 56 44988.0 57 53137.5 58 61287.0 59 69269.0 60 77251.0 61 75982.0 62 74713.0 63 79275.0 64 73283.5 65 62730.0 66 57088.5 67 51447.0 68 40333.0 69 29219.0 70 24479.0 71 19739.0 72 17201.0 73 14663.0 74 16002.0 75 17341.0 76 11800.0 77 6259.0 78 4243.0 79 2227.0 80 1436.5 81 646.0 82 461.5 83 277.0 84 152.5 85 28.0 86 17.5 87 7.0 88 5.5 89 3.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 739689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.645299578606684 #Duplication Level Percentage of deduplicated Percentage of total 1 70.37578797060566 8.195471339982072 2 9.770255052879648 2.275550941003584 3 3.8774538826779974 1.354623361980508 4 1.8087045356923113 0.842516246692867 5 1.0808112469380884 0.6293185379260743 6 0.7580770614936324 0.5296820690857915 7 0.5525952239984212 0.4504595850418216 8 0.4202509896794716 0.39151589384187135 9 0.32853875712511177 0.3443339024914525 >10 6.7333031495605935 21.41251255595257 >50 2.780389835034073 22.473498997551676 >100 1.4302464621135607 31.270033757430486 >500 0.06036754547881912 4.530147129401681 >1k 0.0220573723864916 4.5005400918494125 >5k 0.001160914336131137 0.7997955897681323 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 5916 0.7997955897681323 No Hit CTGTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 3658 0.49453216148949086 No Hit CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 3509 0.47438856059776474 No Hit CTGTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 3294 0.44532229085467 No Hit CTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 2721 0.36785730219051516 No Hit CTGTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC 2336 0.3158084005575316 No Hit CTGTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG 1561 0.21103463753009713 No Hit CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 1555 0.21022348581633635 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC 1457 0.19697467449157685 No Hit CTGTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG 1456 0.1968394825392834 No Hit CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 1358 0.18359067121452394 No Hit CTGTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT 1304 0.1762903057906769 No Hit CTGTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC 1257 0.16993628403288408 No Hit CTGTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG 1239 0.16750282889160176 No Hit CTGTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 1171 0.1583097761356462 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 1140 0.15411882561454882 No Hit CTGTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA 1118 0.1511446026640926 No Hit CTGTGTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGG 1075 0.14533134871547365 No Hit CTGTGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG 1041 0.1407348223374959 No Hit CTGTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA 1040 0.14059963038520243 No Hit CTGTGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG 956 0.12924350639255147 No Hit CTGTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 923 0.12478217196686715 No Hit CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA 923 0.12478217196686715 No Hit CTGTGTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG 890 0.12032083754118285 No Hit CTGTGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT 878 0.11869853411366128 No Hit CTGTGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 848 0.11464277554485737 No Hit CTGTGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 831 0.11234451235586848 No Hit CTGTGTGGGACCTCTGAAGACATTTAAGAGGTTCTCCTGCCCCCGGGCGTGG 818 0.11058701697605346 No Hit CTGTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 795 0.10747760207330379 No Hit CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT 793 0.10720721816871684 No Hit CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA 792 0.1070720262164234 No Hit CTGTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA 773 0.10450337912284756 No Hit CTGTGTGGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCG 758 0.10247549983844562 No Hit CTGTGTGGGATTGCCTTTTGGCACTCGGGAGACAAGATGCAGGTTCCGTCTC 751 0.10152915617239136 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.7038390458692773E-4 0.0 0.0 0.0 0.0 8 0.0016223034275215664 0.0 0.0 0.0 0.0 9 0.0027038390458692775 0.0 0.0 0.0 0.0 10 0.017980529655030695 0.0 0.0 0.0 0.0 11 0.05569908434490712 0.0 0.0 0.0 0.0 12 0.06732559224214502 0.0 0.0 0.0 0.0 13 0.07638345304580708 0.0 0.0 0.0 0.0 14 0.0901730321797404 0.0 0.0 0.0 0.0 15 0.10193473202927177 0.0 0.0 0.0 0.0 16 0.12370063634851945 0.0 0.0 0.0 0.0 17 0.13411041667511617 0.0 0.0 0.0 0.0 18 0.1433034694310717 0.0 0.0 0.0 0.0 19 0.14979268314115798 0.0 0.0 0.0 0.0 20 0.15952650370628738 0.0 0.0 0.0 0.0 21 0.16885474841453638 0.0 0.0 0.0 0.0 22 0.1796701045980135 0.0 0.0 0.0 0.0 23 0.18980950102002328 0.0 0.0 0.0 0.0 24 0.19967851353744615 0.0 0.0 0.0 0.0 25 0.21454962828972718 0.0 0.0 0.0 0.0 26 0.22915035913742127 0.0 0.0 0.0 0.0 27 0.24388628193740883 0.0 0.0 0.0 0.0 28 0.2613260437832657 0.0 0.0 0.0 0.0 29 0.2772786941538944 0.0 0.0 0.0 0.0 30 0.297557486997914 0.0 0.0 0.0 0.0 31 0.3195937752217486 0.0 0.0 0.0 0.0 32 0.34487467030062635 0.0 0.0 0.0 0.0 33 0.3650182711923525 0.0 0.0 0.0 0.0 34 0.38975839846205634 0.0 0.0 0.0 0.0 35 0.4212581233464334 0.0 0.0 0.0 0.0 36 0.4493780494234739 0.0 0.0 0.0 0.0 37 0.4760108640252863 0.0 0.0 0.0 0.0 38 0.5072402050050765 0.0 0.0 0.0 0.0 39 0.5340082115591823 0.0 0.0 0.0 0.0 40 0.5652375525389725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTATA 20 6.309987E-4 46.000004 13 TAATATC 35 1.0189069E-7 46.000004 39 AACAACT 20 6.309987E-4 46.000004 13 AGCCGAT 35 1.0189069E-7 46.000004 29 AGTTTAT 20 6.309987E-4 46.000004 15 CGTATAC 20 6.309987E-4 46.000004 20 GGTCGAC 40 5.6061253E-9 46.000004 9 TTAGCTA 20 6.309987E-4 46.000004 34 TTAGATG 40 5.6061253E-9 46.000004 20 GACCGTT 20 6.309987E-4 46.000004 43 TAGCGAT 20 6.309987E-4 46.000004 24 TAAGTTG 20 6.309987E-4 46.000004 17 ATGCTAG 20 6.309987E-4 46.000004 11 GTCTAAA 20 6.309987E-4 46.000004 11 TAAGTCG 20 6.309987E-4 46.000004 43 CCTAAAT 40 5.6061253E-9 46.000004 40 ATCACAA 20 6.309987E-4 46.000004 35 ATACTTA 20 6.309987E-4 46.000004 14 CCGTCGA 20 6.309987E-4 46.000004 44 AGTATAC 35 1.0189069E-7 46.000004 40 >>END_MODULE