FastQCFastQC Report
Fri 17 Jun 2016
SRR1527510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970128
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC68180.7027938581300612No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT47590.4905538238253096No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG45440.4683917998449689No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG37990.3915978097735556No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC35830.369332706611911No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA32770.33779047713291444No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT29430.30336203057740835No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA24780.25543021127109No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG23780.24512229314069897No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA22040.22718651559381856No Hit
GGGCGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC18720.19296422740092029No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG18700.19275806903831247No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC17110.17636847921099072No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT16240.1674005904375505No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC14200.1463724374515528No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC13180.13585836095855391No Hit
GGGCGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG12730.13121979779987797No Hit
GGGCGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG12690.1308074810746623No Hit
GGGCGAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT12270.12647815545989807No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC11940.12307654247686903No Hit
GGGCGAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGG11890.12256114657034949No Hit
GGGCGAGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC11680.12039648376296735No Hit
GGGCGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA11460.11812874177428133No Hit
GGGCGAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC11390.11740718750515396No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC11140.1148302079725562No Hit
GGGCGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA10590.10916085300084113No Hit
GGGCGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA10470.1079239028251942No Hit
GGGCGAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC10220.10534692329259643No Hit
GGGCGAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG10100.10410997311694951No Hit
GGGCGAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCC9920.10225454785347914No Hit
GGGCGAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCG9830.10132683522174393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC650.044.0000049
CGAACAA700.044.044
CGAAATT502.7284841E-1144.020
CATGCCG207.8558736E-444.023
GCGGTAT207.8558736E-444.021
TAGACCT254.4423254E-544.019
GTTTACG254.4423254E-544.011
CGGTTAT207.8558736E-444.019
CGCATAT302.5273075E-644.037
TAACGGT254.4423254E-544.040
ATATCGC254.4423254E-544.029
ATATCGA207.8558736E-444.024
ACGTGCA351.4457692E-744.021
GTCGTAA408.310963E-944.012
CAAATGC207.8558736E-444.043
CGTACTT351.4457692E-744.016
TCGTATT207.8558736E-444.022
CGTGTAA207.8558736E-444.025
TTAGTAC408.310963E-944.023
GTATACC254.4423254E-544.017
Produced by FastQC (version 0.11.5)