##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527510_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	970128
Sequences flagged as poor quality	0
Sequence length	50
%GC	63
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8105394339716	31.0	31.0	33.0	28.0	34.0
2	31.550519106757047	31.0	31.0	34.0	28.0	34.0
3	32.481560165256546	34.0	31.0	34.0	31.0	34.0
4	35.927537397126976	37.0	35.0	37.0	35.0	37.0
5	35.95100337275081	37.0	35.0	37.0	35.0	37.0
6	33.13385037850676	35.0	32.0	35.0	28.0	37.0
7	35.42456768591361	35.0	35.0	37.0	35.0	37.0
8	36.27799424405852	37.0	35.0	37.0	35.0	37.0
9	38.49231957020105	39.0	39.0	39.0	37.0	39.0
10	37.35653851862847	39.0	37.0	39.0	34.0	39.0
11	36.94995814985239	39.0	37.0	39.0	34.0	39.0
12	36.64720119406924	38.0	35.0	39.0	33.0	39.0
13	36.67180310227104	39.0	35.0	39.0	33.0	39.0
14	37.41175700526116	39.0	37.0	40.0	33.0	40.0
15	37.50727532861643	40.0	37.0	40.0	33.0	40.0
16	37.70069207362327	40.0	37.0	40.0	33.0	40.0
17	37.60949689113189	40.0	37.0	40.0	33.0	40.0
18	37.44958191084063	39.0	36.0	40.0	33.0	40.0
19	37.35123406395857	39.0	36.0	40.0	33.0	40.0
20	37.14741559876635	39.0	36.0	40.0	33.0	40.0
21	37.035758167994324	38.0	35.0	40.0	33.0	40.0
22	36.82742586545281	38.0	35.0	40.0	32.0	40.0
23	36.593760823314035	38.0	35.0	40.0	32.0	40.0
24	36.42454397770191	38.0	35.0	40.0	31.0	40.0
25	36.17464808767503	38.0	35.0	40.0	31.0	40.0
26	35.55625237082117	38.0	34.0	39.0	30.0	40.0
27	35.3970723450926	37.0	34.0	39.0	30.0	40.0
28	35.18012262299408	37.0	34.0	38.0	30.0	40.0
29	34.84691710784556	36.0	33.0	38.0	29.0	40.0
30	34.47827503174839	36.0	33.0	38.0	28.0	40.0
31	34.26398269094388	36.0	33.0	38.0	28.0	39.0
32	34.12511132551581	35.0	33.0	38.0	28.0	39.0
33	33.58793272640311	35.0	32.0	38.0	27.0	39.0
34	33.02951156960731	34.0	31.0	37.0	26.0	39.0
35	32.80766661718866	34.0	31.0	37.0	25.0	38.0
36	32.398431959494005	34.0	31.0	36.0	24.0	38.0
37	32.128311934095294	34.0	30.0	36.0	24.0	38.0
38	31.630553906288654	33.0	30.0	36.0	23.0	38.0
39	30.77103227615325	33.0	29.0	35.0	22.0	38.0
40	30.34104571767849	32.0	28.0	35.0	21.0	37.0
41	29.89456339781967	32.0	27.0	34.0	21.0	37.0
42	29.56692209687794	31.0	27.0	34.0	20.0	36.0
43	29.084921783517228	31.0	26.0	34.0	20.0	36.0
44	28.595564708986856	30.0	26.0	33.0	18.0	36.0
45	28.143669701317766	30.0	25.0	33.0	18.0	35.0
46	27.937458768327478	30.0	24.0	33.0	18.0	35.0
47	27.43560437385582	29.0	24.0	32.0	18.0	34.0
48	26.901762447841936	29.0	23.0	32.0	17.0	34.0
49	26.312575247802354	28.0	23.0	31.0	15.0	34.0
50	29.724278651889236	31.0	27.0	34.0	22.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	7.0
12	16.0
13	48.0
14	94.0
15	134.0
16	247.0
17	406.0
18	657.0
19	1040.0
20	1583.0
21	2252.0
22	3218.0
23	4597.0
24	6448.0
25	8580.0
26	11451.0
27	15015.0
28	20736.0
29	27971.0
30	37602.0
31	51239.0
32	77647.0
33	114960.0
34	159740.0
35	234821.0
36	168998.0
37	20258.0
38	357.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.3120495439777	0.513025085349562	0.09916217241436182	0.07576319825837415
8	98.8454100902149	0.8914287599162173	0.14307390364982764	0.12008724621905562
9	98.07685171441294	1.1095443075552915	0.38139297082446855	0.4322110072072964
10	69.50072567743638	20.8324056207016	3.4846948031599956	6.182173898702027
11	37.29002770768394	26.806771889894943	17.597574753022283	18.305625649398845
12	32.90462701829037	17.392859498952713	25.789895766331867	23.91261771642505
13	26.796567050945853	16.89746095360612	27.729433641746244	28.576538353701782
14	25.95379166460508	17.454707007735063	29.99686639288836	26.594634934771495
15	27.38545841373509	17.06537693995019	28.239160193294083	27.310004453020632
16	31.766117460788678	17.426875628783005	24.11083898207247	26.696167928355845
17	33.741423812115514	18.463027559249916	20.78736001847179	27.00818861016278
18	31.860744149225667	18.92296686622796	22.32344597826266	26.892843006283705
19	32.98008097900483	19.39238945788597	21.22338495538733	26.404144607721868
20	30.548649250408193	21.57395725100193	21.841447726485576	26.035945772104302
21	33.4247645671499	19.061402206719112	22.724527072716178	24.78930615341481
22	31.951350749591807	18.955952204245214	23.30816139725892	25.78453564890406
23	31.524706018174918	18.75474164233998	22.618973991060972	27.101578348424127
24	31.075590025233783	19.328171127933633	22.174084244553296	27.422154602279285
25	30.37805320535022	19.733169231276698	22.01132221727442	27.87745534609866
26	28.7826967163096	19.0689269869543	21.802071479227482	30.346304817508617
27	28.487890257780418	18.88895073639767	23.960240298187454	28.662918707634454
28	30.742025794534328	19.155307340886978	20.88353289462834	29.219133969950356
29	31.124964953078354	20.635318226048522	20.610991539260798	27.628725281612322
30	31.028688997740506	19.451556907954416	22.572072963567695	26.94768113073739
31	32.00866277439678	19.205816139725894	21.940609898899936	26.844911186977388
32	32.93307687233025	20.18836689591477	21.401505780680488	25.477050451074497
33	35.24493675061435	19.898611317269474	20.454105025316245	24.402346906799927
34	31.14238533471872	21.49726634011182	23.772017713126516	23.588330612042945
35	32.46757128956179	23.147254795243512	22.883269011924202	21.501904903270496
36	31.70323916019329	22.818535286065345	21.0169173552356	24.461308198505762
37	30.468350568172447	23.72439513136411	20.107449738591196	25.69980456187225
38	33.13686441376808	21.764447578051556	20.52883743176158	24.56985057641878
39	31.739316873649663	22.183876766777168	20.059414840103575	26.017391519469594
40	31.775188428743423	20.754477759635844	20.422356637474643	27.047977174146094
41	28.3082232447677	21.200604456319166	22.55052941467518	27.940642884237953
42	27.77386076888823	20.28495208879653	22.68525390463939	29.25593323767585
43	27.781385549123417	18.818856893111015	25.161525077103224	28.238232480662344
44	28.604678970197746	18.46704764732077	24.285970511107813	28.642302871373676
45	28.97329012254053	19.715955007998947	23.308367555621526	28.002387313839
46	28.930409183118105	21.358212524532846	21.947619283228605	27.76375900912045
47	27.862715023172203	21.171845364735375	23.978176075734336	26.987263536358093
48	29.885437797898835	20.51801411772467	24.35080731614797	25.245740768228526
49	29.214392327610376	20.432664555605033	22.741638216812625	27.611304899971962
50	28.11494977982287	20.942184948790263	23.150141012320024	27.79272425906684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	10.0
10	20.0
11	24.0
12	28.0
13	32.5
14	37.0
15	89.5
16	142.0
17	415.0
18	688.0
19	679.5
20	671.0
21	877.5
22	1084.0
23	1182.5
24	1281.0
25	1308.0
26	1335.0
27	1440.5
28	1546.0
29	1434.0
30	1322.0
31	1759.5
32	2197.0
33	2387.5
34	2578.0
35	3929.5
36	5281.0
37	5234.5
38	5188.0
39	7437.0
40	9686.0
41	9017.0
42	8348.0
43	10647.0
44	12946.0
45	13965.0
46	14984.0
47	16480.5
48	17977.0
49	18958.5
50	19940.0
51	22915.5
52	25891.0
53	28813.0
54	31735.0
55	35011.5
56	38288.0
57	41590.5
58	44893.0
59	51592.5
60	58292.0
61	73711.0
62	89130.0
63	95694.5
64	102259.0
65	104484.0
66	106709.0
67	103979.0
68	101249.0
69	93137.5
70	85026.0
71	73079.0
72	61132.0
73	51916.0
74	42700.0
75	36648.5
76	30597.0
77	25396.0
78	20195.0
79	16996.0
80	13797.0
81	10877.5
82	7958.0
83	5144.0
84	2330.0
85	1420.5
86	511.0
87	309.0
88	107.0
89	70.5
90	34.0
91	22.5
92	11.0
93	7.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	970128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.77648374076656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06365216121657	8.48656427976824
2	9.691791524844644	2.2827045062246425
3	3.499213088503324	1.236252781267108
4	1.525233086654116	0.7184753058344557
5	0.864394805163317	0.5089765684304441
6	0.4969153451349197	0.3511149289511272
7	0.3612274403581753	0.2977792354667742
8	0.32686600088390355	0.30794657158549427
9	0.23964290562306992	0.25399357034941245
>10	6.803391106238438	22.540578879371807
>50	2.707436008798559	21.95342629882675
>100	1.335656683806904	29.961589814821725
>500	0.05902968193605711	4.724300809424346
>1k	0.024669120809098492	5.668891206916258
>5k	8.810400288963746E-4	0.7074052427614079
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	6818	0.7027938581300612	No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4759	0.4905538238253096	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4544	0.4683917998449689	No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	3799	0.3915978097735556	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3583	0.369332706611911	No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3277	0.33779047713291444	No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2943	0.30336203057740835	No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	2478	0.25543021127109	No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2378	0.24512229314069897	No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2204	0.22718651559381856	No Hit
GGGCGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	1872	0.19296422740092029	No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1870	0.19275806903831247	No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1711	0.17636847921099072	No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1624	0.1674005904375505	No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1420	0.1463724374515528	No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1318	0.13585836095855391	No Hit
GGGCGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1273	0.13121979779987797	No Hit
GGGCGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG	1269	0.1308074810746623	No Hit
GGGCGAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	1227	0.12647815545989807	No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1194	0.12307654247686903	No Hit
GGGCGAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGG	1189	0.12256114657034949	No Hit
GGGCGAGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	1168	0.12039648376296735	No Hit
GGGCGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	1146	0.11812874177428133	No Hit
GGGCGAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC	1139	0.11740718750515396	No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1114	0.1148302079725562	No Hit
GGGCGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1059	0.10916085300084113	No Hit
GGGCGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1047	0.1079239028251942	No Hit
GGGCGAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC	1022	0.10534692329259643	No Hit
GGGCGAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG	1010	0.10410997311694951	No Hit
GGGCGAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCC	992	0.10225454785347914	No Hit
GGGCGAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCG	983	0.10132683522174393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.1624527897349628	0.0	0.0	0.0	0.0
7	0.16317434400409017	0.0	0.0	0.0	0.0
8	0.16368973991060973	0.0	0.0	0.0	0.0
9	0.1643082149984332	0.0	0.0	0.0	0.0
10	0.17605924166707898	0.0	0.0	0.0	0.0
11	0.20110748272392923	0.0	0.0	0.0	0.0
12	0.21337390529909456	0.0	0.0	0.0	0.0
13	0.22213563570992695	0.0	0.0	0.0	0.0
14	0.23316510810944535	0.0	0.0	0.0	0.0
15	0.24285455115201293	0.0	0.0	0.0	0.0
16	0.25099780647502185	0.0	0.0	0.0	0.0
17	0.2584195075289034	0.0	0.0	0.0	0.0
18	0.268108950571471	0.0	0.0	0.0	0.0
19	0.2755306516253525	0.0	0.0	0.0	0.0
20	0.2856324113931358	0.0	0.0	0.0	0.0
21	0.2918171622713704	0.0	0.0	0.0	0.0
22	0.2981049923309089	0.0	0.0	0.0	0.0
23	0.30264047630828095	0.0	0.0	0.0	0.0
24	0.3116083650817212	0.0	0.0	0.0	0.0
25	0.3200608579486418	0.0	0.0	0.0	0.0
26	0.32810103409034685	0.0	0.0	0.0	0.0
27	0.33490426005640495	0.0	0.0	0.0	0.0
28	0.34624296999983506	0.0	0.0	0.0	0.0
29	0.35211848333415796	0.0	0.0	0.0	0.0
30	0.36211716392063725	0.0	0.0	0.0	0.0
31	0.36933270661191103	0.0	0.0	0.0	0.0
32	0.3781975162040473	0.0	0.0	0.0	0.0
33	0.38489766298880146	0.0	0.0	0.0	0.0
34	0.392319364042683	0.0	0.0	0.0	0.0
35	0.4058227367934953	0.0	0.0	0.0	0.0
36	0.419944584632131	0.0	0.0	0.0	0.0
37	0.42922171094948297	0.0	0.0	0.0	0.0
38	0.4390142331733544	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAC	65	0.0	44.000004	9
CGAACAA	70	0.0	44.0	44
CGAAATT	50	2.7284841E-11	44.0	20
CATGCCG	20	7.8558736E-4	44.0	23
GCGGTAT	20	7.8558736E-4	44.0	21
TAGACCT	25	4.4423254E-5	44.0	19
GTTTACG	25	4.4423254E-5	44.0	11
CGGTTAT	20	7.8558736E-4	44.0	19
CGCATAT	30	2.5273075E-6	44.0	37
TAACGGT	25	4.4423254E-5	44.0	40
ATATCGC	25	4.4423254E-5	44.0	29
ATATCGA	20	7.8558736E-4	44.0	24
ACGTGCA	35	1.4457692E-7	44.0	21
GTCGTAA	40	8.310963E-9	44.0	12
CAAATGC	20	7.8558736E-4	44.0	43
CGTACTT	35	1.4457692E-7	44.0	16
TCGTATT	20	7.8558736E-4	44.0	22
CGTGTAA	20	7.8558736E-4	44.0	25
TTAGTAC	40	8.310963E-9	44.0	23
GTATACC	25	4.4423254E-5	44.0	17
>>END_MODULE