FastQCFastQC Report
Fri 17 Jun 2016
SRR1527509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806837
Sequences flagged as poor quality0
Sequence length52
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA64650.8012770857062828No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG40510.5020840640674634No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG34720.43032235755177317No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT32120.3980977570438639No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC30700.38049816753569804No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC26260.3254684651298837No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT22400.27762732745275687No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC22190.27502457125788726No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG21430.265605072647883No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG19730.24453514154655775No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC15280.18938149836955914No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC15140.18764632757297944No Hit
GGGCGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT14310.1773592435646853No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT13720.17004673806481357No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG13140.16285817333612612No Hit
GGGCGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG13010.16124694331073067No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC11700.14501070228559176No Hit
GGGCGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA11410.14141641992124804No Hit
GGGCGAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG10920.13534332213321898No Hit
GGGCGAGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT10450.12951810588755847No Hit
GGGCGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG9950.12332106732834514No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT9850.12208165961650247No Hit
GGGCGAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG9820.12170983730294967No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC9710.12034648881992273No Hit
GGGCGAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT9370.11613250259965768No Hit
GGGCGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA9310.11538885797255206No Hit
GGGCGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA9280.11501703565899929No Hit
GGGCGAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC9100.11278610177768249No Hit
GGGCGAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCCCT9060.11229033869294541No Hit
GGGCGAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA9000.11154669406583982No Hit
GGGCGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG8930.11067910866754994No Hit
GGGCGAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGGG8720.10807635247268034No Hit
GGGCGAGGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAA8710.1079524117014961No Hit
GGGCGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC8590.1064651224472849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA206.31033E-446.027
AGCGTAT301.8607079E-646.027
CTATGCA206.31033E-446.040
ACCGGTA206.31033E-446.037
ACACGCG301.8607079E-646.012
CGTATGA301.8607079E-646.029
CGAAATT405.6079443E-946.020
CGAAACT301.8607079E-646.034
ACGTTGT206.31033E-446.037
GCGAATA206.31033E-446.034
TAGGTTA206.31033E-446.012
ATAGCCT253.4162436E-546.037
CGCGAAT253.4162436E-546.016
AAAACGC206.31033E-446.035
CGTTAGG501.6370905E-1146.028
ATATCGG253.4162436E-546.030
TTAAGTG405.6079443E-946.041
GTTCGAA253.4162436E-546.011
CGCTATT206.31033E-446.038
CGAGGTA301.8607079E-646.04
Produced by FastQC (version 0.11.5)