##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	806837
Sequences flagged as poor quality	0
Sequence length	52
%GC	63
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69785470919157	33.0	31.0	33.0	30.0	34.0
2	32.2909447633165	34.0	31.0	34.0	31.0	34.0
3	32.82538976274018	34.0	31.0	34.0	31.0	34.0
4	36.21976186020225	37.0	37.0	37.0	35.0	37.0
5	36.17852800503695	37.0	37.0	37.0	35.0	37.0
6	34.141603570485735	35.0	35.0	37.0	31.0	37.0
7	35.768426584303896	37.0	35.0	37.0	35.0	37.0
8	36.46211440476825	37.0	37.0	37.0	35.0	37.0
9	38.62733612861086	39.0	39.0	39.0	39.0	39.0
10	37.76381102998499	39.0	38.0	39.0	35.0	39.0
11	37.537296876568625	39.0	37.0	39.0	35.0	39.0
12	37.28060934240745	39.0	37.0	39.0	35.0	39.0
13	37.2720326410415	39.0	37.0	39.0	35.0	39.0
14	37.98515437442755	40.0	38.0	40.0	34.0	40.0
15	38.08728156988339	40.0	38.0	40.0	34.0	40.0
16	38.25024757169044	40.0	38.0	40.0	35.0	40.0
17	38.189322006799394	40.0	38.0	40.0	35.0	40.0
18	38.03915041080169	40.0	38.0	40.0	34.0	40.0
19	37.91250277317476	40.0	37.0	40.0	34.0	40.0
20	37.75735247639858	40.0	37.0	40.0	33.0	40.0
21	37.720048783087535	40.0	37.0	40.0	33.0	40.0
22	37.69709495226421	40.0	37.0	40.0	33.0	40.0
23	37.55519392392763	40.0	36.0	40.0	33.0	40.0
24	37.47016311844896	39.0	36.0	40.0	33.0	40.0
25	37.44469700819372	39.0	36.0	40.0	33.0	40.0
26	37.382152776831006	39.0	36.0	40.0	33.0	40.0
27	37.32090992356573	39.0	35.0	40.0	33.0	40.0
28	37.196671694530615	39.0	35.0	40.0	33.0	40.0
29	37.063466350700324	39.0	35.0	40.0	33.0	40.0
30	36.854208470855944	39.0	35.0	40.0	32.0	40.0
31	36.75409903115499	39.0	35.0	40.0	32.0	40.0
32	36.569071572077135	38.0	35.0	40.0	32.0	40.0
33	36.32367380276314	38.0	35.0	40.0	31.0	40.0
34	35.86452777946475	38.0	34.0	40.0	30.0	40.0
35	35.66179166300009	38.0	34.0	40.0	30.0	40.0
36	35.47417260239677	37.0	34.0	40.0	29.0	40.0
37	35.237869606872266	37.0	34.0	40.0	29.0	40.0
38	35.41628730462287	37.0	34.0	40.0	29.0	40.0
39	35.25017568604316	36.0	34.0	40.0	29.0	40.0
40	34.91954136957031	36.0	34.0	39.0	28.0	40.0
41	34.73556121000896	35.0	33.0	39.0	28.0	40.0
42	34.6010396151887	35.0	33.0	39.0	28.0	40.0
43	34.53850653849538	35.0	33.0	39.0	28.0	40.0
44	34.344221943217775	35.0	33.0	39.0	27.0	40.0
45	34.14585845716049	35.0	33.0	38.0	27.0	40.0
46	34.0458915493464	35.0	33.0	38.0	27.0	40.0
47	33.80721384864601	35.0	33.0	38.0	26.0	40.0
48	33.54983348557391	35.0	33.0	37.0	26.0	40.0
49	33.408057637416235	35.0	33.0	37.0	26.0	40.0
50	33.86049846499355	35.0	33.0	37.0	28.0	40.0
51	33.88923041456948	35.0	33.0	37.0	28.0	40.0
52	33.55950954158027	35.0	33.0	36.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	13.0
13	16.0
14	33.0
15	63.0
16	74.0
17	132.0
18	247.0
19	378.0
20	650.0
21	879.0
22	1306.0
23	1931.0
24	2688.0
25	3583.0
26	4731.0
27	5944.0
28	7742.0
29	10335.0
30	14061.0
31	19743.0
32	27504.0
33	39457.0
34	72267.0
35	85907.0
36	133289.0
37	171778.0
38	177545.0
39	24533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.32427491550338	0.5048107610335173	0.10745664861675902	0.06345767484634443
8	98.8648264767233	0.8841934616285568	0.14686981385335576	0.10411024779478384
9	98.09143606453348	1.0870845040572013	0.40826090028097367	0.41321853112834434
10	69.6141847733805	20.78970101767767	3.4042811621182474	6.191833046823584
11	37.19648454396613	26.843959808486723	17.563150921437664	18.396404726109488
12	32.79435623304335	17.31055962976413	25.92419534552828	23.970888791664237
13	26.89948527397727	16.9239883644404	27.739060057979493	28.437466303602832
14	26.02929711949254	17.385791677872977	29.95983079605918	26.625080406575307
15	27.46477913134871	17.066768132844675	28.27262507792776	27.19582765787885
16	31.86851867229688	17.429542770101026	24.008814667646625	26.693123889955466
17	33.9165159753457	18.23032409272257	20.82762689366006	27.02553303827167
18	31.824767580068837	18.84841671861851	22.31863437100678	27.008181330305874
19	33.12825267061377	19.32781962155925	21.25323950190683	26.290688205920155
20	30.795935238468243	21.3685044191082	21.754579921347187	26.080980421076376
21	33.416414963617186	19.052299287216627	22.787874130710417	24.74341161845577
22	32.02926985252287	18.871345761287593	23.339410562480403	25.759973823709124
23	31.64976321115665	18.59136355918234	22.63431151521311	27.124561714447896
24	31.01989621199821	19.13558748545245	22.210310136991733	27.6342061655576
25	30.512482694769822	19.628871754765832	21.95338091832675	27.905264632137595
26	28.88476854680685	18.94620598708289	21.863152036904605	30.305873429205647
27	28.513937759423523	18.722864717408843	24.02195238939216	28.74124513377547
28	30.883561363695517	18.998261110980287	20.909179921099305	29.208997604224894
29	31.196883633249346	20.51194974945373	20.575159542757707	27.716007074539217
30	30.99895022166807	19.30080053344108	22.624892016603106	27.075357228287743
31	32.15816825455452	19.03395605308135	21.919545087793445	26.888330604570687
32	33.02922399443754	19.993877325903497	21.528264073164717	25.448634606494245
33	35.21591102044155	19.568140776885542	20.557807834791912	24.658140367880996
34	31.23691650234186	21.326240616134363	23.77828483324389	23.658558048279886
35	32.671283047257376	22.87773118981901	22.89768565397968	21.553300108943937
36	31.757963504400514	22.66542064878036	20.977223404479467	24.599392442339653
37	30.683397018232927	23.573658620018666	20.011352974640477	25.731591387107926
38	33.31552717587319	21.599901838909222	20.47650268889503	24.608068296322553
39	31.745693368053274	21.964907410046884	20.13417827888409	26.15522094301575
40	31.860462522169907	20.604657446299562	20.58259598902876	26.952284042501766
41	28.360994847782138	21.07513661371504	22.655753268627986	27.908115269874834
42	28.025363239415146	20.22961267269597	22.678806252068263	29.066217835820616
43	27.96909412929749	18.64924389932539	25.26247556817548	28.119186403201642
44	28.661551217903984	18.37322780189803	24.308379511598996	28.656841468598987
45	28.996315240872693	19.443332420302987	23.621871580009348	27.938480758814976
46	29.088279293091418	21.132521190773353	22.03877610967271	27.740423406462522
47	28.019290141627117	20.925540102895628	24.053929108357696	27.001240647119555
48	29.81432433068885	20.376978249634064	24.500239825392242	25.308457594284846
49	29.440023201712368	20.29691251144903	22.693927026152743	27.56913726068586
50	28.283036102707236	21.039689553156336	22.894463193928885	27.782811150207536
51	27.011899553441403	21.969865040894256	22.279964850397292	28.738270555267047
52	28.65646964628543	20.914385433489045	23.165273778966508	27.263871141259017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	8.0
10	16.0
11	13.0
12	10.0
13	16.0
14	84.5
15	147.0
16	420.0
17	693.0
18	737.0
19	781.0
20	906.5
21	1032.0
22	936.0
23	840.0
24	803.5
25	767.0
26	857.5
27	948.0
28	1002.0
29	1056.0
30	1271.5
31	1487.0
32	1547.0
33	1607.0
34	2343.5
35	3080.0
36	3615.0
37	4150.0
38	4076.5
39	6323.5
40	8644.0
41	8004.0
42	7364.0
43	9526.0
44	11688.0
45	12142.0
46	12596.0
47	12546.0
48	12496.0
49	14873.5
50	17251.0
51	19665.0
52	22079.0
53	23054.5
54	24030.0
55	27213.5
56	30397.0
57	32054.0
58	33711.0
59	40251.0
60	46791.0
61	58330.5
62	69870.0
63	76086.5
64	83183.0
65	84063.0
66	84071.0
67	84079.0
68	74548.0
69	65017.0
70	61727.0
71	58437.0
72	48430.0
73	38423.0
74	34164.5
75	29906.0
76	25082.0
77	20258.0
78	16863.0
79	13468.0
80	11385.0
81	9302.0
82	5884.5
83	2467.0
84	1767.5
85	1068.0
86	702.5
87	337.0
88	225.0
89	71.5
90	30.0
91	18.5
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	806837.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.281754555133194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.95448244271128	6.986913093970654
2	9.743602107115734	2.0036265069648516
3	3.5825789264317662	1.10505591587892
4	1.6225273334378052	0.6672971120560907
5	0.853454199163422	0.4387503299923033
6	0.6232144363947587	0.3844642722135946
7	0.5038755017659751	0.3626506964851637
8	0.3773039044324168	0.3103476910454032
9	0.3471678098291886	0.32125447890961867
>10	9.837626722277806	27.718981653047642
>50	2.9822679219354606	20.92157399821773
>100	1.4814904106946973	28.542320196024722
>500	0.066299408127102	4.736396570806743
>1k	0.022903431898453414	4.699090398680279
>5k	0.0012054437841291271	0.8012770857062828
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6465	0.8012770857062828	No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4051	0.5020840640674634	No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3472	0.43032235755177317	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3212	0.3980977570438639	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3070	0.38049816753569804	No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2626	0.3254684651298837	No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2240	0.27762732745275687	No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	2219	0.27502457125788726	No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2143	0.265605072647883	No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1973	0.24453514154655775	No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1528	0.18938149836955914	No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1514	0.18764632757297944	No Hit
GGGCGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1431	0.1773592435646853	No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1372	0.17004673806481357	No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1314	0.16285817333612612	No Hit
GGGCGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG	1301	0.16124694331073067	No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1170	0.14501070228559176	No Hit
GGGCGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1141	0.14141641992124804	No Hit
GGGCGAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG	1092	0.13534332213321898	No Hit
GGGCGAGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	1045	0.12951810588755847	No Hit
GGGCGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	995	0.12332106732834514	No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	985	0.12208165961650247	No Hit
GGGCGAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	982	0.12170983730294967	No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	971	0.12034648881992273	No Hit
GGGCGAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	937	0.11613250259965768	No Hit
GGGCGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	931	0.11538885797255206	No Hit
GGGCGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	928	0.11501703565899929	No Hit
GGGCGAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC	910	0.11278610177768249	No Hit
GGGCGAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCCCT	906	0.11229033869294541	No Hit
GGGCGAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA	900	0.11154669406583982	No Hit
GGGCGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	893	0.11067910866754994	No Hit
GGGCGAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGGG	872	0.10807635247268034	No Hit
GGGCGAGGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAA	871	0.1079524117014961	No Hit
GGGCGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	859	0.1064651224472849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.18603509754758396	0.0	0.0	0.0	0.0
7	0.18690268294587384	0.0	0.0	0.0	0.0
8	0.18702662371705808	0.0	0.0	0.0	0.0
9	0.18727450525942663	0.0	0.0	0.0	0.0
10	0.19979252314903753	0.0	0.0	0.0	0.0
11	0.2284228412926031	0.0	0.0	0.0	0.0
12	0.237222636046686	0.0	0.0	0.0	0.0
13	0.24750972005498012	0.0	0.0	0.0	0.0
14	0.2597798564022225	0.0	0.0	0.0	0.0
15	0.2699429996393324	0.0	0.0	0.0	0.0
16	0.2801061428764422	0.0	0.0	0.0	0.0
17	0.2886580560881566	0.0	0.0	0.0	0.0
18	0.29795361392697656	0.0	0.0	0.0	0.0
19	0.30749705330816507	0.0	0.0	0.0	0.0
20	0.31716443346053785	0.0	0.0	0.0	0.0
21	0.32571634667225224	0.0	0.0	0.0	0.0
22	0.3337724967992296	0.0	0.0	0.0	0.0
23	0.33996953535844293	0.0	0.0	0.0	0.0
24	0.34790174471423596	0.0	0.0	0.0	0.0
25	0.3557100132988448	0.0	0.0	0.0	0.0
26	0.363270400341085	0.0	0.0	0.0	0.0
27	0.3720701950951679	0.0	0.0	0.0	0.0
28	0.38533185761188443	0.0	0.0	0.0	0.0
29	0.3949992377642572	0.0	0.0	0.0	0.0
30	0.4042947956030772	0.0	0.0	0.0	0.0
31	0.4117312418741332	0.0	0.0	0.0	0.0
32	0.419539510458742	0.0	0.0	0.0	0.0
33	0.42511684516203396	0.0	0.0	0.0	0.0
34	0.43329693606019554	0.0	0.0	0.0	0.0
35	0.44742618397520195	0.0	0.0	0.0	0.0
36	0.45882873492415444	0.0	0.0	0.0	0.0
37	0.4704791674154755	0.0	0.0	0.0	0.0
38	0.4791550213983741	0.0	0.0	0.0	0.0
39	0.48745905306771997	0.0	0.0	0.0	0.0
40	0.4940279139404861	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	20	6.31033E-4	46.0	27
AGCGTAT	30	1.8607079E-6	46.0	27
CTATGCA	20	6.31033E-4	46.0	40
ACCGGTA	20	6.31033E-4	46.0	37
ACACGCG	30	1.8607079E-6	46.0	12
CGTATGA	30	1.8607079E-6	46.0	29
CGAAATT	40	5.6079443E-9	46.0	20
CGAAACT	30	1.8607079E-6	46.0	34
ACGTTGT	20	6.31033E-4	46.0	37
GCGAATA	20	6.31033E-4	46.0	34
TAGGTTA	20	6.31033E-4	46.0	12
ATAGCCT	25	3.4162436E-5	46.0	37
CGCGAAT	25	3.4162436E-5	46.0	16
AAAACGC	20	6.31033E-4	46.0	35
CGTTAGG	50	1.6370905E-11	46.0	28
ATATCGG	25	3.4162436E-5	46.0	30
TTAAGTG	40	5.6079443E-9	46.0	41
GTTCGAA	25	3.4162436E-5	46.0	11
CGCTATT	20	6.31033E-4	46.0	38
CGAGGTA	30	1.8607079E-6	46.0	4
>>END_MODULE