FastQCFastQC Report
Fri 17 Jun 2016
SRR1527508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912196
Sequences flagged as poor quality0
Sequence length52
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA66190.7256116010155712No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG48140.5277374599318567No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG38090.41756376918995475No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT36870.4041894505128284No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC34450.3776600642844301No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC31790.3484996645457774No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT27140.29752377778459893No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC24670.27044626374156433No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG23180.2541120548654017No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG21190.23229656784287586No Hit
GGGCGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT17510.19195436068564212No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT16850.1847190735324426No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC16600.18197843445926096No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC15630.17134475485531617No Hit
GGGCGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG14460.1585185639928261No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC13810.15139290240255382No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG13700.1501870212103539No Hit
GGGCGAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG12120.13286618226784594No Hit
GGGCGAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG11500.12606939736635547No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT11420.12519239286293735No Hit
GGGCGAGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT11320.12409613723366471No Hit
GGGCGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA11090.12157474928633759No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC10980.12036886809413767No Hit
GGGCGAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT10820.1186148590873014No Hit
GGGCGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA10750.11784748014681055No Hit
GGGCGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG10600.11620309670290158No Hit
GGGCGAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGGG10260.11247582756337454No Hit
GGGCGAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCCCT10230.11214695087459275No Hit
GGGCGAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC9950.10907743511262931No Hit
GGGCGAGGGGGAGCTCTGGGCATCTCTGCTTGCACAGTAGACTTGGTGGCAG9930.10885818398677477No Hit
GGGCGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA9870.10820043060921118No Hit
GGGCGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG9710.10644642160237493No Hit
GGGCGAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA9650.10578866822481134No Hit
GGGCGAGGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAA9440.10348653140333877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA206.3107646E-446.00000438
GCGAATA206.3107646E-446.00000433
AATATCG206.3107646E-446.00000446
ATGTACC206.3107646E-446.00000412
ATTCCGA206.3107646E-446.00000433
TATTCGG206.3107646E-446.00000437
ACGGAAT206.3107646E-446.00000410
AACGCCA206.3107646E-446.00000431
GTCGTAA206.3107646E-446.00000412
CGAATAG206.3107646E-446.00000434
ACATATC206.3107646E-446.00000433
CGATATT206.3107646E-446.00000434
CGATACG206.3107646E-446.00000436
AATACGG405.6097633E-946.00000416
CCGTTAT206.3107646E-446.00000444
ACACGCG253.416598E-546.012
TTAGCTA453.092282E-1046.025
TAGGTAC253.416598E-546.022
TACGGGT253.416598E-546.035
TCATGCG301.8609771E-646.038
Produced by FastQC (version 0.11.5)