##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527508_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912196
Sequences flagged as poor quality	0
Sequence length	52
%GC	63
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.167142807028313	31.0	31.0	33.0	26.0	34.0
2	31.312984270924233	31.0	31.0	34.0	28.0	34.0
3	31.911429122688546	34.0	31.0	34.0	30.0	34.0
4	35.59080723879517	37.0	35.0	37.0	33.0	37.0
5	35.70984854132226	37.0	35.0	37.0	33.0	37.0
6	32.96251353875702	35.0	32.0	35.0	28.0	37.0
7	35.355505834272456	35.0	35.0	37.0	35.0	37.0
8	36.18783024700832	37.0	35.0	37.0	35.0	37.0
9	38.453798306504304	39.0	39.0	39.0	37.0	39.0
10	37.23849589342641	39.0	37.0	39.0	34.0	39.0
11	36.814709777284705	39.0	37.0	39.0	32.0	39.0
12	36.52179904318809	38.0	35.0	39.0	32.0	39.0
13	36.493708588943605	39.0	35.0	39.0	32.0	39.0
14	37.20854509337905	39.0	36.0	40.0	33.0	40.0
15	37.33981074242816	39.0	36.0	40.0	33.0	40.0
16	37.47425114777965	40.0	36.0	40.0	33.0	40.0
17	37.45482001675079	40.0	36.0	40.0	33.0	40.0
18	37.3367598630119	39.0	36.0	40.0	33.0	40.0
19	37.17274576954953	39.0	36.0	40.0	32.0	40.0
20	36.941075163670966	39.0	35.0	40.0	31.0	40.0
21	36.984575683296136	39.0	35.0	40.0	31.0	40.0
22	36.98505145823924	39.0	35.0	40.0	32.0	40.0
23	36.93526391258019	39.0	35.0	40.0	32.0	40.0
24	36.857391393954806	39.0	35.0	40.0	32.0	40.0
25	36.80952668066951	39.0	35.0	40.0	32.0	40.0
26	36.67931891830265	38.0	35.0	40.0	31.0	40.0
27	36.577746449228016	38.0	35.0	40.0	31.0	40.0
28	36.36441071874904	38.0	35.0	40.0	31.0	40.0
29	36.265622738972766	38.0	35.0	40.0	31.0	40.0
30	36.12246381260168	38.0	35.0	40.0	30.0	40.0
31	35.99848607097597	38.0	35.0	40.0	30.0	40.0
32	35.82051993211985	38.0	34.0	40.0	30.0	40.0
33	35.64153537178414	38.0	34.0	40.0	30.0	40.0
34	35.25294563887586	37.0	34.0	40.0	28.0	40.0
35	35.0455165337274	37.0	33.0	40.0	28.0	40.0
36	34.89419708045201	36.0	33.0	39.0	28.0	40.0
37	34.70733263465308	36.0	33.0	39.0	28.0	40.0
38	34.6482302049121	36.0	33.0	39.0	28.0	40.0
39	34.39714052681661	35.0	33.0	39.0	27.0	40.0
40	34.53994426636381	35.0	33.0	39.0	27.0	40.0
41	34.58993790808116	35.0	33.0	39.0	28.0	40.0
42	34.63950949138124	35.0	33.0	39.0	28.0	40.0
43	34.560554968449765	35.0	33.0	39.0	29.0	40.0
44	34.410349310893714	35.0	33.0	39.0	28.0	40.0
45	34.28168726896413	35.0	33.0	38.0	28.0	40.0
46	34.01461199128258	35.0	33.0	38.0	28.0	40.0
47	33.874309907081376	35.0	33.0	37.0	27.0	40.0
48	33.716574069607844	35.0	33.0	37.0	27.0	40.0
49	33.5982497182623	35.0	33.0	37.0	27.0	40.0
50	33.458856429977764	35.0	33.0	36.0	27.0	39.0
51	33.258355660406316	35.0	33.0	36.0	26.0	39.0
52	32.75897614109248	35.0	32.0	36.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	25.0
13	26.0
14	37.0
15	73.0
16	145.0
17	217.0
18	402.0
19	648.0
20	972.0
21	1467.0
22	2080.0
23	3007.0
24	4292.0
25	5855.0
26	7668.0
27	10218.0
28	13111.0
29	16920.0
30	22204.0
31	29674.0
32	40215.0
33	57244.0
34	89320.0
35	103322.0
36	154440.0
37	179671.0
38	155864.0
39	13070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.30935895355823	0.5078952330420217	0.10721380054286578	0.07553201285688602
8	98.8448754434354	0.8828146582532701	0.16049182412551688	0.11181807418581095
9	98.09262483062851	1.08288131059553	0.3804007033576117	0.4440931554183531
10	69.90701559752509	20.534073817469054	3.3204486755039486	6.238461909501905
11	36.945130213243644	26.935110436792094	17.639410828374604	18.480348521589658
12	32.62588303390938	17.31963306131577	25.835894917320402	24.21858898745445
13	26.545172309459808	16.856574683511	27.789751325373057	28.808501681656136
14	25.80355537625686	17.26756091892532	29.994211770277442	26.934671934540383
15	27.18812623602822	17.047213537441515	28.396748067301324	27.367912159228936
16	31.610531070077048	17.322702577077735	24.21058632136076	26.85618003148446
17	33.503654916268	18.387714920916117	20.805068209025254	27.303561953790634
18	31.77233840095769	18.902955066674267	22.25486627873834	27.0698402536297
19	32.990826532894246	19.16868743120996	21.167819196751577	26.67266683914422
20	30.548588242000623	21.486719959306992	21.81022499550535	26.15446680318703
21	33.47745440672838	18.921043284557264	22.598652044078246	25.002850264636105
22	31.89183026454841	18.86239360839118	23.30135190244202	25.944424224618395
23	31.518445597218143	18.58734307100667	22.56006384592785	27.33414748584734
24	31.048700060074808	19.03154585198795	22.247192489333433	27.672561598603806
25	30.318155308727512	19.582743182386242	22.1959973514464	27.903104157439852
26	28.95901757955527	18.847703782958924	21.755192962915864	30.438085674569937
27	28.574122228117645	18.733912448640424	23.942551820003597	28.74941350323834
28	30.7748554038825	19.09008590259111	20.917982538840338	29.217076154686055
29	31.084876495840806	20.546242254953977	20.6572929501993	27.711588299005918
30	30.897416783235183	19.326109739573514	22.674841810312696	27.10163166687861
31	32.056816736753944	19.19784783094861	21.837412135111315	26.90792329718613
32	33.00562598388942	20.093269428938516	21.339054326043964	25.56205026112809
33	35.357532810930984	19.707606698560397	20.437274445404277	24.49758604510434
34	31.186718643800237	21.354073028165	23.69216703427772	23.767041293757043
35	32.73485084345908	22.923911089283443	22.793566294962925	21.547671772294553
36	31.828247438050596	22.621015658915407	20.944402299505807	24.60633460352819
37	30.491254072589662	23.435752842590844	20.051282838337375	26.021710246482115
38	33.28637705054616	21.550850913619442	20.5157663484602	24.647005687374204
39	31.700204780551548	22.105556262031406	20.127582230134752	26.066656727282293
40	31.806322325465143	20.542843862503236	20.483755684085438	27.167078127946187
41	28.338098391135237	21.179110629733085	22.52794355599016	27.954847423141516
42	27.75993317225684	20.134269389473314	22.83029085854356	29.275506579726283
43	27.839850207630818	18.489666694438476	25.391144008524485	28.279339089406225
44	28.637705054615452	18.342439563427156	24.359457835815988	28.660397546141397
45	28.9470683931962	19.471253984889213	23.453512183785065	28.12816543812953
46	29.022381154927228	21.189744309337026	22.109941284548494	27.67793325118724
47	27.966906235063515	20.93859214467066	24.02049559524488	27.074006025020935
48	29.85904345118812	20.321509850953085	24.452968440992944	25.36647825686585
49	29.331634867945045	20.122539454240098	22.878087603979846	27.667738073835007
50	28.25840060688712	20.955693732487315	22.917114304381954	27.868791356243616
51	27.165433744502277	21.644361518796398	22.25048125622125	28.939723480480072
52	28.606242518055332	20.881257975259704	23.096242474205106	27.416257032479862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	9.0
10	18.0
11	23.0
12	28.0
13	31.0
14	89.0
15	144.0
16	404.0
17	664.0
18	695.5
19	727.0
20	980.0
21	1233.0
22	1111.0
23	989.0
24	959.5
25	930.0
26	1006.0
27	1082.0
28	1175.0
29	1268.0
30	1492.5
31	1717.0
32	1782.5
33	1848.0
34	2644.5
35	3441.0
36	4088.0
37	4735.0
38	4675.5
39	7309.0
40	10002.0
41	9046.0
42	8090.0
43	10573.0
44	13056.0
45	13538.0
46	14020.0
47	14048.0
48	14076.0
49	16782.0
50	19488.0
51	22092.0
52	24696.0
53	25767.0
54	26838.0
55	30456.0
56	34074.0
57	36168.0
58	38262.0
59	45687.5
60	53113.0
61	65299.0
62	77485.0
63	85345.5
64	94433.5
65	95661.0
66	95638.0
67	95615.0
68	84566.0
69	73517.0
70	69952.0
71	66387.0
72	55205.0
73	44023.0
74	39192.0
75	34361.0
76	28572.0
77	22783.0
78	19204.5
79	15626.0
80	12926.5
81	10227.0
82	6385.0
83	2543.0
84	1828.0
85	1113.0
86	705.5
87	298.0
88	205.5
89	75.5
90	38.0
91	23.0
92	8.0
93	5.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	912196.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.499388289358865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.00469851213782	7.3500651175843785
2	9.662229182980944	2.0289499186578324
3	3.5510310623858	1.1185096185468912
4	1.6204646306447401	0.680555494652465
5	0.8948055338031845	0.4697455371433333
6	0.5679979117723831	0.357817837394595
7	0.4259984338292874	0.31309060772027064
8	0.3174105977551553	0.26660936903911003
9	0.27042547637692504	0.2555371871834562
>10	7.99582354476638	23.59043451188122
>50	3.0832680762203077	22.364162964976824
>100	1.5129209083790134	30.295243566075712
>500	0.06369094231271209	4.536086542804398
>1k	0.028191072826938134	5.647580125323944
>5k	0.0010441138084051162	0.7256116010155712
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6619	0.7256116010155712	No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4814	0.5277374599318567	No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3809	0.41756376918995475	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3687	0.4041894505128284	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3445	0.3776600642844301	No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3179	0.3484996645457774	No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2714	0.29752377778459893	No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	2467	0.27044626374156433	No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2318	0.2541120548654017	No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2119	0.23229656784287586	No Hit
GGGCGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1751	0.19195436068564212	No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1685	0.1847190735324426	No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1660	0.18197843445926096	No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1563	0.17134475485531617	No Hit
GGGCGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG	1446	0.1585185639928261	No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1381	0.15139290240255382	No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1370	0.1501870212103539	No Hit
GGGCGAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG	1212	0.13286618226784594	No Hit
GGGCGAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1150	0.12606939736635547	No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1142	0.12519239286293735	No Hit
GGGCGAGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	1132	0.12409613723366471	No Hit
GGGCGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1109	0.12157474928633759	No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1098	0.12036886809413767	No Hit
GGGCGAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	1082	0.1186148590873014	No Hit
GGGCGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1075	0.11784748014681055	No Hit
GGGCGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1060	0.11620309670290158	No Hit
GGGCGAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGGG	1026	0.11247582756337454	No Hit
GGGCGAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCCCT	1023	0.11214695087459275	No Hit
GGGCGAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC	995	0.10907743511262931	No Hit
GGGCGAGGGGGAGCTCTGGGCATCTCTGCTTGCACAGTAGACTTGGTGGCAG	993	0.10885818398677477	No Hit
GGGCGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	987	0.10820043060921118	No Hit
GGGCGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	971	0.10644642160237493	No Hit
GGGCGAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA	965	0.10578866822481134	No Hit
GGGCGAGGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAA	944	0.10348653140333877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.15194103021719016	0.0	0.0	0.0	0.0
7	0.1530372858464628	0.0	0.0	0.0	0.0
8	0.15336616253524463	0.0	0.0	0.0	0.0
9	0.15391429034988094	0.0	0.0	0.0	0.0
10	0.16410946770211665	0.0	0.0	0.0	0.0
11	0.18680195922806064	0.0	0.0	0.0	0.0
12	0.1942564975071147	0.0	0.0	0.0	0.0
13	0.20203991247495057	0.0	0.0	0.0	0.0
14	0.2127832176418226	0.0	0.0	0.0	0.0
15	0.22210139049064018	0.0	0.0	0.0	0.0
16	0.23032330771018508	0.0	0.0	0.0	0.0
17	0.23854522492973002	0.0	0.0	0.0	0.0
18	0.24665751658634766	0.0	0.0	0.0	0.0
19	0.25159066691807463	0.0	0.0	0.0	0.0
20	0.2593740818859105	0.0	0.0	0.0	0.0
21	0.2657323645356919	0.0	0.0	0.0	0.0
22	0.27121364268205517	0.0	0.0	0.0	0.0
23	0.2762564185767094	0.0	0.0	0.0	0.0
24	0.2829435779152726	0.0	0.0	0.0	0.0
25	0.2889729838762722	0.0	0.0	0.0	0.0
26	0.2957697687777627	0.0	0.0	0.0	0.0
27	0.30355318374559853	0.0	0.0	0.0	0.0
28	0.31396761222368874	0.0	0.0	0.0	0.0
29	0.321093273813961	0.0	0.0	0.0	0.0
30	0.32843818653008783	0.0	0.0	0.0	0.0
31	0.33348096242474207	0.0	0.0	0.0	0.0
32	0.3422510074589233	0.0	0.0	0.0	0.0
33	0.34882854123455925	0.0	0.0	0.0	0.0
34	0.35705045845410416	0.0	0.0	0.0	0.0
35	0.368122640309758	0.0	0.0	0.0	0.0
36	0.3796333244171209	0.0	0.0	0.0	0.0
37	0.3878552416366658	0.0	0.0	0.0	0.0
38	0.39443277541230176	0.0	0.0	0.0	0.0
39	0.40397019938697387	0.0	0.0	0.0	0.0
40	0.4105477331626098	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	20	6.3107646E-4	46.000004	38
GCGAATA	20	6.3107646E-4	46.000004	33
AATATCG	20	6.3107646E-4	46.000004	46
ATGTACC	20	6.3107646E-4	46.000004	12
ATTCCGA	20	6.3107646E-4	46.000004	33
TATTCGG	20	6.3107646E-4	46.000004	37
ACGGAAT	20	6.3107646E-4	46.000004	10
AACGCCA	20	6.3107646E-4	46.000004	31
GTCGTAA	20	6.3107646E-4	46.000004	12
CGAATAG	20	6.3107646E-4	46.000004	34
ACATATC	20	6.3107646E-4	46.000004	33
CGATATT	20	6.3107646E-4	46.000004	34
CGATACG	20	6.3107646E-4	46.000004	36
AATACGG	40	5.6097633E-9	46.000004	16
CCGTTAT	20	6.3107646E-4	46.000004	44
ACACGCG	25	3.416598E-5	46.0	12
TTAGCTA	45	3.092282E-10	46.0	25
TAGGTAC	25	3.416598E-5	46.0	22
TACGGGT	25	3.416598E-5	46.0	35
TCATGCG	30	1.8609771E-6	46.0	38
>>END_MODULE