FastQCFastQC Report
Fri 17 Jun 2016
SRR1527507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140166
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC9320.6649258736070088No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6810.4858524891913874No Hit
ACACCCGGGTGTAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG6770.4829987300771942Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG6550.46730305494913177No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG6010.4287773069075239No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT5960.42521010801478243No Hit
ACACCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA5480.39096499864446443No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGA5470.3902515588659161No Hit
ACACCCGGGTGTAGCACTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG5000.3567198892741464Illumina Single End Adapter 2 (100% over 30bp)
ACACCCGGGTGTAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCT4680.333889816360601Illumina Single End Adapter 2 (96% over 29bp)
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC4510.32176134012528No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC4280.3053522252186693No Hit
ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC4030.28751623075496197No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG3900.27824151363383415No Hit
ACACCCGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGAGCACTGACTGCT3870.2761011942981893No Hit
ACACCCGGGGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGACCA3810.27182055562689955No Hit
ACACCCGGACATGATGTCTGAGGAAAAGGCCAAGGATGAGCAGAGTGCTG3700.2639727180628683No Hit
ACACCCGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCA3660.26111895894867515No Hit
ACACCCGGGGTTGTGTGATCCTTTGCTGGGGTGCAAAAGGCACCGGACAC3570.2546980009417405No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC3340.23828888603512977No Hit
ACACCCGGGCATGGCGTTCACACACCTCCCAGTGTCCTTGTGCCTCTGCT3320.23686200647803318No Hit
ACACCCGGCGGCTGGCCAGATGATAGTAGCACATGCCTCTAATCCCAGTA3150.2247335302427122No Hit
ACACCCGGGGTGGGGCTGAAGGATCTGTCCTTGCCGCCACCACGCTGACC3150.2247335302427122No Hit
ACACCCGGGCGATGCATAGGTGTGACCCATAGCTTTCTTGTCCTTGTAAG3150.2247335302427122No Hit
ACACCCGGGGGGAGACTGCACCATGGCCCCGGACCCCTGGTTCTCCACAT3100.22116633134997074No Hit
ACACCCGGGGGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCCTTG3040.216885692678681No Hit
ACACCCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACCGACTGTT2960.21117817445029463No Hit
ACACCCGGGGCCCTTTGCAGGACCCCATGAGCAAGCTGTGGCGGCGCGGG2930.20903785511464978No Hit
ACACCCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC2900.2068975357790049No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT2880.20547065622190833No Hit
ACACCCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAG2820.20119001755061855No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC2770.19762281865787706No Hit
ACACCCGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGAGCACTGGCTGCT2760.1969093788793288No Hit
ACACCCCCTCATTCTCTCCTTTATGCCAAAGAAGTGAACATTCCAGGGAG2740.19548249932223222No Hit
ACACCCGGGGAACCTGGCCTCCCCCAGGCAACAAAGGGACCACAGCACTG2740.19548249932223222No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA2720.1940556197651356No Hit
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT2700.19262874020803902No Hit
ACACCCGGGAGCGAGCACGGACATAGCTGGGCTGGAGGAGAGCTTCCGGA2580.18406746286545952No Hit
ACACCCGGGCTGTCCTGTGTCCCTGTGAATCTGCTCTTGGCTGGCTTTCT2580.18406746286545952No Hit
ACACCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG2580.18406746286545952No Hit
ACACCCGGGGGAGGGATCTGCGGAACAGCTTTTCTCCTTCCTAGGTTAGG2580.18406746286545952No Hit
ACACCCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA2540.18121370375126636No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG2490.1776465048585249No Hit
ACACCCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGC2480.1769330650799766No Hit
ACACCCGGGACCCAAAGTTGAAAGCAAAGAGAAAGTGTTAGTGAAAAAAG2470.17621962530142832No Hit
ACACCCGGGGTATAGATACGGGATCAAATAACTCAGCATGTACTATGGAA2370.1690852275159454No Hit
ACACCCGGGGTAGAAAACAGGATTAAGGAAGAAATGGAGACCTTTCCGGA2330.1662314684017522No Hit
ACACCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT2280.16266426950901075No Hit
ACACCCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC2260.16123738995191414No Hit
ACACCCGGGAGGTACAGCTTTTAAAAGCAAGGGCTGCATCTAGCTTCTTA2230.15909707061626926No Hit
ACACCCGGGATTTCCCCCACCCCCCAACCCCTGTGATGCTCCCAAAGGAA2210.1576701910591727No Hit
ACACCCGGGTCCTCCAGCGTTGAACACTTCCTTGCTTTTTTCACATGTTT2200.15695675128062442No Hit
ACACCCGGGTGTAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2190.15624331150207613Illumina Single End Adapter 2 (96% over 26bp)
ACACCCGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGC2180.15552987172352783No Hit
ACACCCGGGAAAGCTGGTGTGTGGAAGGAAAAGAAGCTCAAGTAACACTT2170.15481643194497952No Hit
ACACCCGGGGAGCATTCGGAGTGAGACGTCGAGCTGAGCGATCGCAGCGG2130.15196267283078635No Hit
ACACCCGGGGTGTAAAATGTATCGCTGCTGTCCAGCATGCTTTTCTTCTT2120.15124923305223806No Hit
ACACCCGGGAGGAATAGAGAACCTGTTTTTGAGACATGTTGTCATTTTGA2110.15053579327368977No Hit
ACACCCGGGGACGTTAACAGCGCTCGGGACTCGGCGTGCGCGTGCCTTCC2110.15053579327368977No Hit
ACACCCGGGAGGAACCAAGGACTGCCAGAAACTCTCTGCCTCTACGTTAT2070.1476820341594966No Hit
ACACCCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG2060.1469685943809483No Hit
ACACCCGGGCTCTAGATAGCTCTAAGTGCTGGAGGAGAGCATGAGACCAT2040.14554171482385173No Hit
ACACCCGGGGCAGAATTCGGACATGATTCAGGATTTGAAGTCCGCCATCA2000.14268795570965856No Hit
ACACCCGGGACTGCAGCTGCTATTGGAGCGATGTTTGGCCTTACCACCTG1980.14126107615256195No Hit
ACACCCGGGCAAAGAATAAATGCAGCAGCTACACTCAGGAAACTGGGTCA1970.14054763637401366No Hit
ACACCCGGGGTGGGAAGGACTGTAATCATAATAAGCTCACTACTTGGCTA1960.13983419659546537No Hit
ACACCCGGGGGCCAAAGCGGGCTTGGAACCTCCGCTCCAGGACAACACTA1930.1376938772598205No Hit
ACACCCGGGCAAAAGCTTCAGTTACTTTGTGGCATGGTGGAAGAAGAATG1930.1376938772598205No Hit
ACACCCGGGACCACCACCCCTATCTTCTCCCCCACCCCATACTCTATCCC1890.13484011814562732No Hit
ACACCCGGGGAAGCCTAGCCAGGCTCCCAGCTCTGGCTGCTCACCATATA1880.13412667836707903No Hit
ACACCCGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGA1880.13412667836707903No Hit
ACACCCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG1850.13198635903143416No Hit
ACACCCGGGGTTTTGCCTCAGCTTCCCGAAGCGCTTGCTTTATACGTGTG1850.13198635903143416No Hit
ACACCCGGGATCCATCCTCAGATGAGCCACCTGTACCCTTCTGACAGTGC1830.13055947947433758No Hit
ACACCCGGGGTCAGGCACAGCTCTCTATGCCTTCTACCCTAGAACTCAGG1820.12984603969578928No Hit
ACACCCGGGATCTGTTACTGTTTCATGTTTTTATATTCCATATGTAAAAT1790.12770572036014438No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT1790.12770572036014438No Hit
ACACCCGGGGGATGGCCCTCATTCAGTGCGTTCTGTGGTTCCAGTAAGGA1770.1262788408030478No Hit
ACACCCGGGAGTTATGTGACACATGAAACCAAACACTTCATCTACTTCTA1750.12485196124595124No Hit
ACACCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA1750.12485196124595124No Hit
ACACCCGGGGGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG1730.12342508168885465No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC1720.12271164191030635No Hit
ACACCCGGGGTCCCCGGCCTGGCTTCCCGGCGCGGATTGTGAGGGGTTCT1710.12199820213175806No Hit
ACACCCGGGCTGACGTAATGACTAGTATCCTGCTGTTTACACCCGAGTGT1700.12128476235320977No Hit
ACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTT1680.11985788279611317No Hit
ACACCCGGGCGCGGAGACCCGCATAAGCCTGACCCCTTGCTTCCTCCCCG1670.11914444301756488No Hit
ACACCCGGGAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTGTTCCTATGA1630.1162906839033717No Hit
ACACCCGGGAGTGTCTTCTTTCTTACCAAAGAAGGCAAGCGGCAGCTCAA1630.1162906839033717No Hit
ACACCCGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTG1610.11486380434627515No Hit
ACACCCCCTTTGCATCTGCGGACCAACACCATTTACTGACGAGGGGATAA1600.11415036456772684No Hit
ACACCCGGGGGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCTCTG1590.11343692478917855No Hit
ACACCCGGGGATTCAACCAACACCAGTTACTAAGTAGGGTTGAGTGGGGC1580.11272348501063026No Hit
ACACCCAGGAGTCTCCGCGCGCTCCCGCGGTTCCTCTCCCCACTGTGGCC1570.11201004523208197No Hit
ACACCCGGGGCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT1560.11129660545353368No Hit
ACACCCGGGAAGCTAAGACTGTGGATGCCACGTCAGCCACTGAAGCCCGC1560.11129660545353368No Hit
ACACCCGGGAAGTTAGGAATTCATGGTTACGCCTTCGCCATCACGAATAA1520.1084428463393405No Hit
ACACCCGGGTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC1510.1077294065607922No Hit
ACACCCGGGGTTTATATAAAATGCTGACCTCAGGTTATAGTTCTTAAATG1510.1077294065607922No Hit
ACACCCGGGGAAGCCAGTGCTAAATACTGAGCCAATGCTTTCTGAAAGAA1470.10487564744659902No Hit
ACACCCGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACTGACTGC1470.10487564744659902No Hit
ACACCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC1460.10416220766805073No Hit
ACACCCGGGGGCAGAAGCGGCTTAGCGTGTGTCCTAATCTTCTCTCTGCG1430.10202188833240587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCC454.7293724E-1044.00000418
TTTCGTG454.7293724E-1044.00000416
CGTGACG454.7293724E-1044.00000419
TTATGCC454.7293724E-1044.00000421
CGTTGAA454.7293724E-1044.00000418
TGAACAC454.7293724E-1044.00000421
CTGACGC454.7293724E-1044.00000442
CGTTCCG454.7293724E-1044.00000419
GGAACTT454.7293724E-1044.0000048
GTGGTCG454.7293724E-1044.00000414
CGTCATG454.7293724E-1044.00000429
AGGTGTG454.7293724E-1044.00000418
GCATAAG408.223651E-944.021
TCACGAA254.4227978E-544.041
CGGGTTA207.8328163E-444.06
AGCGTTG502.7284841E-1144.016
TTTAGAA254.4227978E-544.025
CGGGTGA408.223651E-944.06
AAACTCT408.223651E-944.029
CTAGAAA207.8328163E-444.012
Produced by FastQC (version 0.11.5)