FastQCFastQC Report
Fri 17 Jun 2016
SRR1527506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117526
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8210.6985688273233157No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG6350.5403059748481187No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG6070.516481459421745No Hit
ACACCCGGGTGTAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA5850.4977621973010228Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5640.47989381073124243No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5020.4271395265728435No Hit
ACACCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG5020.4271395265728435No Hit
ACACCCGGGTGTAGCACTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT4930.41948164661436615Illumina Single End Adapter 2 (100% over 32bp)
ACACCCGGGTGTAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC4200.35736773139560607Illumina Single End Adapter 2 (96% over 31bp)
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC4160.3539642291918384No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG4120.3505607269880707No Hit
ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3820.32503446045981316No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT3420.2909994384221364No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT3350.28504330956554297No Hit
ACACCCGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCC3200.27228017630141416No Hit
ACACCCGGGGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATG3190.2714293007504723No Hit
ACACCCGGACATGATGTCTGAGGAAAAGGCCAAGGATGAGCAGAGTGCTGTC3060.2603679185882273No Hit
ACACCCGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT2910.24760478532409852No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC2860.24335040756938892No Hit
ACACCCGGGCATGGCGTTCACACACCTCCCAGTGTCCTTGTGCCTCTGCTTC2840.24164865646750505No Hit
ACACCCGGGGTTGTGTGATCCTTTGCTGGGGTGCAAAAGGCACCGGACACAG2740.23313990095808587No Hit
ACACCCGGGGGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCCTTGTC2700.2297363987543182No Hit
ACACCCGGGGGGAGACTGCACCATGGCCCCGGACCCCTGGTTCTCCACATAC2690.22888552320337627No Hit
ACACCCGGGCGATGCATAGGTGTGACCCATAGCTTTCTTGTCCTTGTAAGAG2640.22463114544866666No Hit
ACACCCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGTG2610.22207851879584092No Hit
ACACCCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT2570.21867501659207322No Hit
ACACCCGGCGGCTGGCCAGATGATAGTAGCACATGCCTCTAATCCCAGTACT2520.21442063883736362No Hit
ACACCCGGGGCCCTTTGCAGGACCCCATGAGCAAGCTGTGGCGGCGCGGGAG2500.2127188877354798No Hit
ACACCCGGGGAACCTGGCCTCCCCCAGGCAACAAAGGGACCACAGCACTGGT2490.21186801218453788No Hit
ACACCCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACCGACTGTTCT2460.20931538553171214No Hit
ACACCCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC2420.20591188332794447No Hit
ACACCCGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGAGCACTGGCTGCTCC2380.20250838112417677No Hit
ACACCCGGGGTGGGGCTGAAGGATCTGTCCTTGCCGCCACCACGCTGACCCA2360.20080663002229296No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC2320.1974031278185253No Hit
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT2250.19144699896193185No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG2240.19059612341098991No Hit
ACACCCGGGAGCGAGCACGGACATAGCTGGGCTGGAGGAGAGCTTCCGGAAG2210.18804349675816415No Hit
ACACCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG2190.1863417456562803No Hit
ACACCCGGGTGTAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2190.1863417456562803Illumina Single End Adapter 2 (96% over 26bp)
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC2170.18463999455439647No Hit
ACACCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC2130.1812364923506288No Hit
ACACCCGGGAGGAATAGAGAACCTGTTTTTGAGACATGTTGTCATTTTGATG2070.1761312390449773No Hit
ACACCCGGGACCCAAAGTTGAAAGCAAAGAGAAAGTGTTAGTGAAAAAAGAC2030.17272773684120962No Hit
ACACCCGGGATTTCCCCCACCCCCCAACCCCTGTGATGCTCCCAAAGGAATG2030.17272773684120962No Hit
ACACCCGGGCTGTCCTGTGTCCCTGTGAATCTGCTCTTGGCTGGCTTTCTCT2000.17017511018838385No Hit
ACACCCGGGAGGTACAGCTTTTAAAAGCAAGGGCTGCATCTAGCTTCTTATC1990.16932423463744192No Hit
ACACCCGGGGGAGGGATCTGCGGAACAGCTTTTCTCCTTCCTAGGTTAGGCT1970.1676224835355581No Hit
ACACCCGGGGTATAGATACGGGATCAAATAACTCAGCATGTACTATGGAAAC1940.16506985688273232No Hit
ACACCCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1910.16251723022990658No Hit
ACACCCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC1900.16166635467896465No Hit
ACACCCCCTCATTCTCTCCTTTATGCCAAAGAAGTGAACATTCCAGGGAGTG1900.16166635467896465No Hit
ACACCCGGGGTAGAAAACAGGATTAAGGAAGAAATGGAGACCTTTCCGGAAA1860.15826285247519697No Hit
ACACCCGGGGAGCATTCGGAGTGAGACGTCGAGCTGAGCGATCGCAGCGGAG1830.15571022582237123No Hit
ACACCCGGGGAAGCCTAGCCAGGCTCCCAGCTCTGGCTGCTCACCATATAGG1820.1548593502714293No Hit
ACACCCGGGGACGTTAACAGCGCTCGGGACTCGGCGTGCGCGTGCCTTCCAA1790.15230672361860353No Hit
ACACCCGGGACCACCACCCCTATCTTCTCCCCCACCCCATACTCTATCCCTC1790.15230672361860353No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC1790.15230672361860353No Hit
ACACCCGGGGCAGAATTCGGACATGATTCAGGATTTGAAGTCCGCCATCAAA1790.15230672361860353No Hit
ACACCCGGGAAAGCTGGTGTGTGGAAGGAAAAGAAGCTCAAGTAACACTTTT1750.14890322141483586No Hit
ACACCCGGGGTTTTGCCTCAGCTTCCCGAAGCGCTTGCTTTATACGTGTGTG1740.14805234586389393No Hit
ACACCCGGGATCCATCCTCAGATGAGCCACCTGTACCCTTCTGACAGTGCAG1730.14720147031295203No Hit
ACACCCGGGAGGAACCAAGGACTGCCAGAAACTCTCTGCCTCTACGTTATGC1730.14720147031295203No Hit
ACACCCGGGAGTGTCTTCTTTCTTACCAAAGAAGGCAAGCGGCAGCTCAAGT1710.1454997192110682No Hit
ACACCCGGGAAGTTAGGAATTCATGGTTACGCCTTCGCCATCACGAATAATG1710.1454997192110682No Hit
ACACCCGGGGGCCAAAGCGGGCTTGGAACCTCCGCTCCAGGACAACACTAAG1700.14464884366012626No Hit
ACACCCGGGAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTGTTCCTATGAAA1680.14294709255824242No Hit
ACACCCGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTC1680.14294709255824242No Hit
ACACCCGGGCAAAAGCTTCAGTTACTTTGTGGCATGGTGGAAGAAGAATGCC1650.14039446590541668No Hit
ACACCCGGGCGCGGAGACCCGCATAAGCCTGACCCCTTGCTTCCTCCCCGCC1630.13869271480353285No Hit
ACACCCGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAG1630.13869271480353285No Hit
ACACCCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT1620.13784183925259091No Hit
ACACCCGGGGTCAGGCACAGCTCTCTATGCCTTCTACCCTAGAACTCAGGTT1620.13784183925259091No Hit
ACACCCGGGATCTGTTACTGTTTCATGTTTTTATATTCCATATGTAAAATTA1610.136990963701649No Hit
ACACCCGGGGTGGGAAGGACTGTAATCATAATAAGCTCACTACTTGGCTATT1610.136990963701649No Hit
ACACCCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT1580.13443833704882324No Hit
ACACCCGGGAGTTATGTGACACATGAAACCAAACACTTCATCTACTTCTACC1580.13443833704882324No Hit
ACACCCGGGGTGTAAAATGTATCGCTGCTGTCCAGCATGCTTTTCTTCTTTT1560.1327365859469394No Hit
ACACCCGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAA1550.13188571039599747No Hit
ACACCCCCTTTGCATCTGCGGACCAACACCATTTACTGACGAGGGGATAAGG1520.1293330837431717No Hit
ACACCCGGGTCCTCCAGCGTTGAACACTTCCTTGCTTTTTTCACATGTTTTA1500.12763133264128787No Hit
ACACCCGGGGATTCAACCAACACCAGTTACTAAGTAGGGTTGAGTGGGGCTG1480.12592958153940403No Hit
ACACCCGGGCAAAGAATAAATGCAGCAGCTACACTCAGGAAACTGGGTCAGT1470.12507870598846213No Hit
ACACCCGGGCTCTAGATAGCTCTAAGTGCTGGAGGAGAGCATGAGACCATGA1440.12252607933563636No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT1440.12252607933563636No Hit
ACACCCGGGACTGCAGCTGCTATTGGAGCGATGTTTGGCCTTACCACCTGTG1420.12082432823375253No Hit
ACACCCGGGTGTAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA1420.12082432823375253Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGTCCCCGGCCTGGCTTCCCGGCGCGGATTGTGAGGGGTTCTCT1400.11912257713186869No Hit
ACACCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA1390.11827170158092677No Hit
ACACCCGGGGGTTCCGGGTTGCTGCGGCCAGGCTGCAGAAACGATGGCGGCT1390.11827170158092677No Hit
ACACCCGGGTGTAGCACTGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1370.11656995047904294Illumina Single End Adapter 1 (96% over 26bp)
ACACCCGGGGCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCG1370.11656995047904294No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA1360.115719074928101No Hit
ACACCCGGGTGTAGCACTGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1350.1148681993771591TruSeq Adapter, Index 20 (96% over 25bp)
ACACCCGGGGGATGGCCCTCATTCAGTGCGTTCTGTGGTTCCAGTAAGGATT1330.11316644827527526No Hit
ACACCCGGGCTGACGTAATGACTAGTATCCTGCTGTTTACACCCGAGTGTTA1320.11231557272433333No Hit
ACACCCGGGGTTTATATAAAATGCTGACCTCAGGTTATAGTTCTTAAATGTG1320.11231557272433333No Hit
ACACCCGGGGTTTGAGACAGTCTTGCTACGGAGACCCAGGACTCACTTCTGC1320.11231557272433333No Hit
ACACCCGGGGGAGCAACGCCGAAGACAATGGCAGGCTCGGCGTTGGCAGTTC1310.11146469717339141No Hit
ACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTA1310.11146469717339141No Hit
ACACCCGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACTGACTGCTT1300.1106138216224495No Hit
ACACCCGGGGGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGT1290.10976294607150758No Hit
ACACCCGGGGATGCCTTAAACTTATGAGTAAGGAAAATAACGATTCGGGGTG1280.10891207052056565No Hit
ACACCCGGGGGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCTCTGAG1280.10891207052056565No Hit
ACACCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA1270.10806119496962374No Hit
ACACCCGGGGAAGCCAGTGCTAAATACTGAGCCAATGCTTTCTGAAAGAACA1260.10721031941868181No Hit
ACACCCGGGAGTTGTAGCTCCAAACTTCAGTTTAGGGGTAGAGCCAAATTCA1250.1063594438677399No Hit
ACACCCGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAG1240.10550856831679797No Hit
ACACCCAGGAGTCTCCGCGCGCTCCCGCGGTTCCTCTCCCCACTGTGGCCGC1220.10380681721491414No Hit
ACACCCGGGGTACCATCCAGTTTGTGGACTGGTGCCCCACTGGCTTCAAGGT1210.10295594166397223No Hit
ACACCCGGGGTCATTATGTGGTGGTTTTGAATTCTGGAAGATATCTTCAGTC1210.10295594166397223No Hit
ACACCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA1210.10295594166397223No Hit
ACACCCGGGTGTAGCACTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA1190.10125419056208838Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC1190.10125419056208838No Hit
ACACCCGGGGGCAGAAGCGGCTTAGCGTGTGTCCTAATCTTCTCTCTGCGTG1180.10040331501114648No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTATC206.288233E-446.00000436
CTAGAAC206.288233E-446.00000439
CGGGTAC206.288233E-446.0000046
AGGTAAG206.288233E-446.00000428
CAGCGAC206.288233E-446.00000415
GATACAT206.288233E-446.00000422
ACGCCGG206.288233E-446.00000427
ATTAGGG206.288233E-446.00000430
GATAAAC206.288233E-446.00000437
TGCCACA206.288233E-446.00000411
CGACGGG206.288233E-446.00000436
CGACGAT405.5370037E-946.00000433
ACCATAT206.288233E-446.00000443
TGGACAA206.288233E-446.00000412
ACCGCCG206.288233E-446.00000411
TAGGTAA206.288233E-446.00000427
GAACTGC206.288233E-446.00000415
TTATGCC206.288233E-446.00000421
GCTATAA206.288233E-446.00000421
ACCGATC206.288233E-446.00000440
Produced by FastQC (version 0.11.5)