FastQCFastQC Report
Fri 17 Jun 2016
SRR1527505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152969
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC10170.6648405886159942No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG8620.5635128686204394No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG7330.4791820564951068No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT7100.46414633030221814No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG6390.4177316972719963No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC6190.4046571527564409No Hit
ACACCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG6130.4007347894017742No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC5550.36281861030666346No Hit
ACACCCGGGTGTAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA5120.3347083395982193Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT5110.33405461237244144No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT4930.3222875223084416No Hit
ACACCCGGGGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATG4810.3144427955991083No Hit
ACACCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4570.2987533421804418No Hit
ACACCCGGACATGATGTCTGAGGAAAAGGCCAAGGATGAGCAGAGTGCTGTC4310.2817564343102197No Hit
ACACCCGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCC4210.27521916205244196No Hit
ACACCCGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT4140.2706430714719976No Hit
ACACCCGGGGTTGTGTGATCCTTTGCTGGGGTGCAAAAGGCACCGGACACAG4040.2641057992142199No Hit
ACACCCGGGGGTTTCCGGTGCGGGCAGTCGCAGCCGGCGGTAAAGCCTTGTC3960.2588759814079977No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC3920.2562610725048866No Hit
ACACCCGGCGGCTGGCCAGATGATAGTAGCACATGCCTCTAATCCCAGTACT3620.23664925573155343No Hit
ACACCCGGGCGATGCATAGGTGTGACCCATAGCTTTCTTGTCCTTGTAAGAG3490.2281508017964424No Hit
ACACCCGGGCATGGCGTTCACACACCTCCCAGTGTCCTTGTGCCTCTGCTTC3480.22749707457066465No Hit
ACACCCGGGGAACCTGGCCTCCCCCAGGCAACAAAGGGACCACAGCACTGGT3460.2261896201191091No Hit
ACACCCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC3420.22357471121599803No Hit
ACACCCGGGTGTAGCACTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT3400.22226725676444248Illumina Single End Adapter 2 (100% over 32bp)
ACACCCGGGGTGGGGCTGAAGGATCTGTCCTTGCCGCCACCACGCTGACCCA3370.22030607508710917No Hit
ACACCCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACCGACTGTTCT3330.21769116618399806No Hit
ACACCCGGGGCCCTTTGCAGGACCCCATGAGCAAGCTGTGGCGGCGCGGGAG3320.2170374389582203No Hit
ACACCCGGGGGGAGACTGCACCATGGCCCCGGACCCCTGGTTCTCCACATAC3250.2124613483777759No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG3140.20527034889422038No Hit
ACACCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG3020.19742562218488716No Hit
ACACCCGGGTGTAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC3000.1961181677333316Illumina Single End Adapter 2 (96% over 31bp)
ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT2990.19546444050755385No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC2960.19350325883022051No Hit
ACACCCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT2920.19088834992710943No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC2900.18958089547555387No Hit
ACACCCGGGGTAGAAAACAGGATTAAGGAAGAAATGGAGACCTTTCCGGAAA2840.18565853212088723No Hit
ACACCCGGGAGCGAGCACGGACATAGCTGGGCTGGAGGAGAGCTTCCGGAAG2830.18500480489510945No Hit
ACACCCGGGGGGCTGGCAAGATGGCTCAGCGGGTAAGAGCACTGGCTGCTCC2810.18369735044355393No Hit
ACACCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC2770.1810824415404428No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC2770.1810824415404428No Hit
ACACCCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGTG2740.1791212598631095No Hit
ACACCCGGGACCCAAAGTTGAAAGCAAAGAGAAAGTGTTAGTGAAAAAAGAC2740.1791212598631095No Hit
ACACCCGGGGCAGAATTCGGACATGATTCAGGATTTGAAGTCCGCCATCAAA2720.17781380541155398No Hit
ACACCCGGGGGAGGGATCTGCGGAACAGCTTTTCTCCTTCCTAGGTTAGGCT2680.1751988965084429No Hit
ACACCCCCTCATTCTCTCCTTTATGCCAAAGAAGTGAACATTCCAGGGAGTG2650.17323771483110956No Hit
ACACCCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC2630.17193026037955403No Hit
ACACCCGGGGTATAGATACGGGATCAAATAACTCAGCATGTACTATGGAAAC2630.17193026037955403No Hit
ACACCCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT2560.16735416979910964No Hit
ACACCCGGGCTGTCCTGTGTCCCTGTGAATCTGCTCTTGGCTGGCTTTCTCT2550.16670044257333186No Hit
ACACCCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC2530.1653929881217763No Hit
ACACCCGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTC2490.16277807921866522No Hit
ACACCCGGGGACGTTAACAGCGCTCGGGACTCGGCGTGCGCGTGCCTTCCAA2460.1608168975413319No Hit
ACACCCGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAA2430.1588557158639986No Hit
ACACCCGGGAGGTACAGCTTTTAAAAGCAAGGGCTGCATCTAGCTTCTTATC2360.15427962528355418No Hit
ACACCCGGGACTGCAGCTGCTATTGGAGCGATGTTTGGCCTTACCACCTGTG2340.15297217083199863No Hit
ACACCCGGGGGCCAAAGCGGGCTTGGAACCTCCGCTCCAGGACAACACTAAG2320.1516647163804431No Hit
ACACCCGGGCAAAGAATAAATGCAGCAGCTACACTCAGGAAACTGGGTCAGT2320.1516647163804431No Hit
ACACCCGGGGTTTTGCCTCAGCTTCCCGAAGCGCTTGCTTTATACGTGTGTG2310.15101098915466532No Hit
ACACCCGGGAGGAACCAAGGACTGCCAGAAACTCTCTGCCTCTACGTTATGC2290.14970353470310976No Hit
ACACCCGGGAGGAATAGAGAACCTGTTTTTGAGACATGTTGTCATTTTGATG2250.14708862579999868No Hit
ACACCCGGGATCCATCCTCAGATGAGCCACCTGTACCCTTCTGACAGTGCAG2250.14708862579999868No Hit
ACACCCGGGTCCTCCAGCGTTGAACACTTCCTTGCTTTTTTCACATGTTTTA2230.14578117134844315No Hit
ACACCCGGGGAGCGCCCTCCGCCTAGCATTCGGAGTGAGACGTCGAGCTGAG2220.14512744412266537No Hit
ACACCCGGGGAAGCCTAGCCAGGCTCCCAGCTCTGGCTGCTCACCATATAGG2220.14512744412266537No Hit
ACACCCGGGCAAAAGCTTCAGTTACTTTGTGGCATGGTGGAAGAAGAATGCC2160.14120508076799876No Hit
ACACCCGGGCGCGGAGACCCGCATAAGCCTGACCCCTTGCTTCCTCCCCGCC2150.14055135354222098No Hit
ACACCCGGGAGTTATGTGACACATGAAACCAAACACTTCATCTACTTCTACC2080.1359752629617766No Hit
ACACCCGGGAAAGCTGGTGTGTGGAAGGAAAAGAAGCTCAAGTAACACTTTT2040.1333603540586655No Hit
ACACCCGGGGTGTAAAATGTATCGCTGCTGTCCAGCATGCTTTTCTTCTTTT2010.13139917238133217No Hit
ACACCCGGGATCTGTTACTGTTTCATGTTTTTATATTCCATATGTAAAATTA2010.13139917238133217No Hit
ACACCCGGGCTCTAGATAGCTCTAAGTGCTGGAGGAGAGCATGAGACCATGA2010.13139917238133217No Hit
ACACCCGGGGTCAGGCACAGCTCTCTATGCCTTCTACCCTAGAACTCAGGTT2000.1307454451555544No Hit
ACACCCGGGATTTCCCCCACCCCCCAACCCCTGTGATGCTCCCAAAGGAATG1990.13009171792977664No Hit
ACACCCGGGAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTGTTCCTATGAAA1970.12878426347822108No Hit
ACACCCGGGGTCCCCGGCCTGGCTTCCCGGCGCGGATTGTGAGGGGTTCTCT1960.1281305362524433No Hit
ACACCCGGGGAGCATTCGGAGTGAGACGTCGAGCTGAGCGATCGCAGCGGAG1960.1281305362524433No Hit
ACACCCGGGCTGACGTAATGACTAGTATCCTGCTGTTTACACCCGAGTGTTA1940.12682308180088775No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT1940.12682308180088775No Hit
ACACCCGGGACCACCACCCCTATCTTCTCCCCCACCCCATACTCTATCCCTC1930.12616935457510997No Hit
ACACCCGGGGGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGT1910.12486190012355446No Hit
ACACCCAGGAGTCTCCGCGCGCTCCCGCGGTTCCTCTCCCCACTGTGGCCGC1890.1235544456719989No Hit
ACACCCGGGGATTCAACCAACACCAGTTACTAAGTAGGGTTGAGTGGGGCTG1890.1235544456719989No Hit
ACACCCGGGAGTGTCTTCTTTCTTACCAAAGAAGGCAAGCGGCAGCTCAAGT1880.12290071844622112No Hit
ACACCCGGGGTTTGAGACAGTCTTGCTACGGAGACCCAGGACTCACTTCTGC1870.12224699122044336No Hit
ACACCCGGGGGATGGCCCTCATTCAGTGCGTTCTGTGGTTCCAGTAAGGATT1860.12159326399466558No Hit
ACACCCGGGAAGCTAAGACTGTGGATGCCACGTCAGCCACTGAAGCCCGCCT1820.1189783550915545No Hit
ACACCCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT1770.11570971896266563No Hit
ACACCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA1740.11374853728533232No Hit
ACACCCGGGGTTTATATAAAATGCTGACCTCAGGTTATAGTTCTTAAATGTG1730.11309481005955455No Hit
ACACCCGGGGGTTCCGGGTTGCTGCGGCCAGGCTGCAGAAACGATGGCGGCT1720.11244108283377677No Hit
ACACCCGGGGGGGACGTACGTGGCTCTGAGACCCTTGCCACCTGCCTCCCCT1710.11178735560799902No Hit
ACACCCGGGGTGGGAAGGACTGTAATCATAATAAGCTCACTACTTGGCTATT1710.11178735560799902No Hit
ACACCCGGGGCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCG1700.11113362838222124No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA1680.10982617393066568No Hit
ACACCCGGGGGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCTCTGAG1660.10851871947911015No Hit
ACACCCCCTTTGCATCTGCGGACCAACACCATTTACTGACGAGGGGATAAGG1650.10786499225333238No Hit
ACACCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC1630.10655753780177683No Hit
ACACCCGGGACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC1610.10525008335022128No Hit
ACACCCGGGAAGTTAGGAATTCATGGTTACGCCTTCGCCATCACGAATAATG1610.10525008335022128No Hit
ACACCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA1580.10328890167288797No Hit
ACACCCGGGGAAGCCAGTGCTAAATACTGAGCCAATGCTTTCTGAAAGAACA1570.10263517444711019No Hit
ACACCCGGGGCACTCCCGACACTTTTCTCTGGCCGCCGCGCCTCCATCCCAT1550.10132771999555466No Hit
ACACCCGGGGGCAGAAGCGGCTTAGCGTGTGTCCTAATCTTCTCTCTGCGTG1550.10132771999555466No Hit
ACACCCGGGGGCCGCGCCCGCGATGGAATAATGCCCAGCGGCCCGCCCGGTC1540.10067399276977688No Hit
ACACCCGGGGGGGCTGGTGAGGTGGCTCAGTGGGTAAGAGCACTGACTGCTT1530.1000202655439991No Hit
ACACCCGGGGAGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC1530.1000202655439991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTC351.01181286E-746.00000422
ATCCTCC351.01181286E-746.00000435
TTAGGAG351.01181286E-746.00000444
CAAACTT351.01181286E-746.00000421
TCACCGC351.01181286E-746.00000441
CATATAG351.01181286E-746.00000445
CTCCGAA351.01181286E-746.00000419
ACGTTAT351.01181286E-746.00000444
TCATTCA351.01181286E-746.00000419
ACCATAT351.01181286E-746.00000443
GTTTAGG351.01181286E-746.00000430
TAGGTGT351.01181286E-746.00000417
TTATGCC351.01181286E-746.00000421
TCTTACA351.01181286E-746.00000417
TTGTAAG351.01181286E-746.00000444
CCTAAGA351.01181286E-746.00000431
CGTTCAC351.01181286E-746.00000415
CGTTATG351.01181286E-746.00000445
CGCCTAA351.01181286E-746.00000429
TTCCATA351.01181286E-746.00000436
Produced by FastQC (version 0.11.5)