FastQCFastQC Report
Fri 17 Jun 2016
SRR1527504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645422
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC37110.5749726535507002No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC31890.49409533607469225No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT31390.4863484665846531No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC23280.3606942434562193No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG20120.3117340282791724No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC18770.2908174806560669No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA18180.2816761746578208No Hit
CGGCAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTC14250.22078578046611363No Hit
CGGCAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC13620.21102472490866442No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA13250.2052920414860355No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC12550.1944464241999808No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA12430.1925871755223714No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG12160.18840386599775033No Hit
CGGCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT11940.1849952434221331No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG11370.17616381220348856No Hit
CGGCAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA11110.17213544006866824No Hit
CGGCAGGGGATTCCCAATATTCTTTAGATGAAAAGATGCGTAGCCACATG10900.16888175488285184No Hit
CGGCAGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACT10650.1650083201378323No Hit
CGGCAGGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC9810.15199357939456667No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG9680.14997939332715648No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT9580.14843001942914869No Hit
CGGCAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCT9380.14533127163313303No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC8510.13185171872046506No Hit
CGGCAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC8500.1316967813306643No Hit
CGGCAGGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA8440.1307671569918596No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG8020.12425978662022677No Hit
CGGCAGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC7710.11945672753640253No Hit
CGGCAGGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCG7680.11899191536700018No Hit
CGGCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC7550.11697772929959005No Hit
CGGCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA7510.11635797974038692No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG7450.11542835540158222No Hit
CGGCAGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGG7290.11294935716476971No Hit
CGGCAGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT7060.10938579719935175No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT7010.10861111025034784No Hit
CGGCAGGGGAAGCAAGGAGGCCCGTGAGGAGGGATGAAGAACAAGAACAA6980.1081462980809455No Hit
CGGCAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG6920.1072166737421408No Hit
CGGCAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT6560.10163892770931265No Hit
CGGCAGGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC6510.10086424076030877No Hit
CGGCAGGGGGGCCCCCTCTCCTCTCTGTTCCAGTGACCTGTGTTCCCTCG6470.10024449120110564No Hit
CGGCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT6460.10008955381130485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT207.8539125E-444.043
AACGTGT454.783942E-1044.035
CTATGCA454.783942E-1044.041
CCTTATT207.8539125E-444.036
ACTATAG408.301868E-944.034
GTACCTA207.8539125E-444.037
AACCGAT551.8189894E-1244.023
ATGATCG207.8539125E-444.032
CGTATCT408.301868E-944.022
CGTATCC207.8539125E-444.013
ATTAGCC254.440665E-544.034
GTACAGT207.8539125E-444.013
ATAGGAT408.301868E-944.010
GGTCGAC207.8539125E-444.09
CCGATTT207.8539125E-444.038
CACGGAT254.440665E-544.023
TTAGCGG207.8539125E-444.044
CGTGCAC600.044.024
ATGGATA408.301868E-944.011
GTTTACG454.783942E-1044.010
Produced by FastQC (version 0.11.5)