##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527504_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645422
Sequences flagged as poor quality	0
Sequence length	50
%GC	63
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.521054441900027	31.0	31.0	33.0	30.0	34.0
2	31.637094799991324	31.0	31.0	34.0	30.0	34.0
3	32.54178661402927	34.0	31.0	34.0	31.0	34.0
4	36.21812085736154	37.0	37.0	37.0	35.0	37.0
5	35.076867537827965	35.0	35.0	37.0	33.0	37.0
6	35.732031136217856	37.0	35.0	37.0	35.0	37.0
7	36.424461824976525	37.0	37.0	37.0	35.0	37.0
8	36.57608045588778	37.0	37.0	37.0	35.0	37.0
9	38.50296085351909	39.0	39.0	39.0	37.0	39.0
10	36.98454034724568	39.0	37.0	39.0	32.0	39.0
11	35.37224172711807	37.0	35.0	39.0	31.0	39.0
12	35.24464768786933	37.0	35.0	39.0	30.0	39.0
13	35.465317265293095	37.0	35.0	39.0	30.0	39.0
14	36.36999978308766	38.0	35.0	40.0	31.0	40.0
15	36.626538915624195	38.0	35.0	40.0	32.0	40.0
16	36.803299236778415	38.0	35.0	40.0	33.0	40.0
17	36.7442820356294	38.0	35.0	40.0	33.0	40.0
18	36.59748815503655	38.0	35.0	40.0	32.0	40.0
19	36.492403419778064	38.0	35.0	40.0	32.0	40.0
20	36.34221486097468	38.0	35.0	40.0	31.0	40.0
21	36.20684296475794	38.0	35.0	40.0	31.0	40.0
22	35.97501014839903	38.0	34.0	40.0	31.0	40.0
23	35.64832931012578	37.0	34.0	40.0	30.0	40.0
24	35.50615566249679	37.0	34.0	39.0	30.0	40.0
25	35.32844247639529	37.0	34.0	39.0	30.0	40.0
26	34.71391740597625	36.0	33.0	39.0	28.0	40.0
27	34.5642385911853	36.0	33.0	38.0	28.0	40.0
28	34.33327962170487	35.0	33.0	38.0	27.0	40.0
29	34.04195394641025	35.0	33.0	38.0	27.0	40.0
30	33.72608773794509	35.0	32.0	38.0	26.0	39.0
31	33.549387842372894	35.0	32.0	38.0	26.0	39.0
32	33.47689418705901	35.0	32.0	38.0	26.0	39.0
33	32.93298338141557	34.0	31.0	37.0	24.0	39.0
34	32.45115443849141	34.0	31.0	37.0	23.0	38.0
35	32.20929407426459	34.0	31.0	36.0	23.0	38.0
36	31.865300532054995	33.0	30.0	36.0	23.0	38.0
37	31.63786948694033	33.0	30.0	36.0	23.0	38.0
38	31.117104777959227	33.0	29.0	35.0	21.0	38.0
39	30.239892659376345	32.0	28.0	35.0	20.0	38.0
40	30.021179941185768	32.0	27.0	34.0	20.0	37.0
41	29.577443904918024	32.0	27.0	34.0	19.0	37.0
42	29.27692579428653	31.0	26.0	34.0	18.0	37.0
43	28.753454329105608	31.0	26.0	34.0	18.0	36.0
44	28.307541422511164	30.0	25.0	33.0	18.0	36.0
45	27.955650721543424	30.0	24.0	33.0	18.0	35.0
46	27.792232059024947	30.0	24.0	33.0	18.0	35.0
47	27.249771467350044	29.0	23.0	32.0	18.0	34.0
48	26.74917650777321	29.0	23.0	32.0	16.0	34.0
49	25.983965529529517	28.0	23.0	31.0	14.0	34.0
50	29.495396810149018	31.0	27.0	34.0	20.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	9.0
14	35.0
15	78.0
16	133.0
17	269.0
18	476.0
19	823.0
20	1297.0
21	2011.0
22	2936.0
23	4133.0
24	5771.0
25	7456.0
26	9916.0
27	12959.0
28	17785.0
29	23257.0
30	31550.0
31	46881.0
32	69396.0
33	78292.0
34	87303.0
35	126035.0
36	101608.0
37	14715.0
38	289.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.78374149006387	1.0013603502824509	0.07979275574740248	0.13510540390628148
8	98.88429585604457	0.6797103290560285	0.30119828577271923	0.1347955291266799
9	97.05649946856475	1.2562323565047364	0.8272107241463724	0.8600574507841382
10	65.49187973140053	22.723737337741817	5.196135241748809	6.588247689108831
11	36.73240143657948	25.08420847135672	17.61932503075507	20.564065061308725
12	29.293392540074553	16.359219239505315	28.905584253403198	25.44180396701693
13	23.861132716269356	15.873955334649267	29.100805364552183	31.164106584529193
14	25.679322985581525	16.198549166281907	30.43837985070233	27.68374799743424
15	26.24252039750737	17.41852617357326	26.720347307652975	29.618606121266396
16	30.313035502353497	17.65418594346025	24.198586351255457	27.834192202930797
17	32.12409865173483	18.94125084053534	22.052858439904437	26.881792067825394
18	31.00870438255901	18.977971001918124	22.306645884398115	27.70667873112475
19	30.40770224752178	18.12860423103024	22.08756441521981	29.37612910622817
20	32.097604358078904	19.420782061968758	22.45213209342105	26.029481486531292
21	34.469076046369665	16.240692136307718	23.115419059158192	26.17481275816442
22	33.51605616170505	17.368791271447208	22.877745103203793	26.23740746364394
23	31.3439888940879	18.838837225877022	22.36459246818361	27.452581411851472
24	30.159492549060925	19.63103209992842	21.928908528063808	28.280566822946845
25	29.395806154732874	20.159833411318488	21.63762623523834	28.8067341987103
26	29.90833904019386	19.22075789173595	21.532888559733014	29.338014508337178
27	31.17417751486624	18.121322173709604	21.858412015704452	28.846088295719703
28	32.92450520744567	19.01871953543573	20.49759692108419	27.559178336034407
29	31.044339982213188	19.330298626325103	21.429235445956287	28.196125945505422
30	30.4517044662252	19.14778238113978	20.9160828109361	29.484430341698918
31	31.96885138715445	20.093985020653154	19.853987003851746	28.083176588340653
32	34.89468905615241	19.447896105183897	19.83152108233063	25.82589375633307
33	34.35023906839246	19.23919544112224	20.81088032326137	25.599685167223925
34	31.819026931217092	21.313342278385306	22.59947755112159	24.268153239276007
35	32.39740820734342	22.23769874593677	22.460498712470294	22.90439433424953
36	31.71506394266077	22.25071348668003	20.788104526960655	25.24611804369854
37	31.546956874726924	22.814530648165075	20.11087319614144	25.527639280966564
38	33.681839168791896	21.440236000632147	20.24380947659051	24.634115353985457
39	32.506329192373364	19.79387129660904	20.960859716588526	26.738939794429072
40	31.45074075566064	21.69355863295642	20.13876192630558	26.716938685077363
41	29.850702331187968	20.04827849066192	22.496908999073476	27.604110179076635
42	29.53401030643517	18.367362748713244	22.854039682564277	29.244587262287308
43	28.661557864466968	18.038895482335587	24.829646339914042	28.469900313283404
44	27.234119692232365	18.262934947987517	25.145873552497434	29.357071807282676
45	27.9541137426366	18.962787137717648	24.34314293593959	28.739956183706166
46	29.767346015475148	18.975337066291512	22.47227395409515	28.785042964138192
47	29.31988683373049	20.474976062173276	23.91117749317501	26.293959610921224
48	30.895290213224836	20.329025040980937	22.859462491207303	25.916222254586923
49	28.94106491566758	20.60853209218155	23.533750011620306	26.91665298053057
50	27.796077605039805	20.85441772979539	23.66761591640818	27.681888748756627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.0
12	3.0
13	2.5
14	2.0
15	44.0
16	86.0
17	128.5
18	171.0
19	317.5
20	464.0
21	468.5
22	473.0
23	502.0
24	531.0
25	523.5
26	516.0
27	652.0
28	788.0
29	906.5
30	1025.0
31	1294.5
32	1564.0
33	1900.0
34	2236.0
35	2409.0
36	2582.0
37	2982.5
38	3383.0
39	4586.5
40	5790.0
41	5933.0
42	6076.0
43	6166.0
44	6256.0
45	8643.0
46	11030.0
47	10862.0
48	10694.0
49	11298.0
50	11902.0
51	14220.5
52	16539.0
53	19381.5
54	22224.0
55	24968.0
56	27712.0
57	29767.0
58	31822.0
59	36080.5
60	40339.0
61	49274.0
62	58209.0
63	60151.0
64	62093.0
65	64456.0
66	66819.0
67	64441.5
68	62064.0
69	60966.5
70	59869.0
71	50297.5
72	40726.0
73	34932.5
74	29139.0
75	24770.0
76	20401.0
77	17963.0
78	15525.0
79	16637.0
80	17749.0
81	11890.0
82	6031.0
83	3779.0
84	1527.0
85	1183.5
86	840.0
87	508.0
88	176.0
89	105.5
90	35.0
91	21.5
92	8.0
93	5.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	645422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.967020027207004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43712660708312	9.522606914545833
2	9.452516369545476	2.4514193814279652
3	3.321703388615399	1.2921778309385177
4	1.525832815561822	0.7914201871023919
5	0.8602972805047078	0.5577746032828134
6	0.5412703723175453	0.42111982547852417
7	0.3524829135401233	0.3199457099386138
8	0.2843760455001673	0.295000790180688
9	0.24375089614300052	0.28446504767423486
>10	5.963532954165272	22.158215864968966
>50	2.798355876308369	24.90711503481443
>100	1.1446733260048751	27.546628407460545
>500	0.052573722697509914	4.572047435631261
>1k	0.021507432012617695	4.880062966555215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3711	0.5749726535507002	No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3189	0.49409533607469225	No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3139	0.4863484665846531	No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2328	0.3606942434562193	No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2012	0.3117340282791724	No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1877	0.2908174806560669	No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1818	0.2816761746578208	No Hit
CGGCAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTC	1425	0.22078578046611363	No Hit
CGGCAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	1362	0.21102472490866442	No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1325	0.2052920414860355	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1255	0.1944464241999808	No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1243	0.1925871755223714	No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1216	0.18840386599775033	No Hit
CGGCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1194	0.1849952434221331	No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1137	0.17616381220348856	No Hit
CGGCAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA	1111	0.17213544006866824	No Hit
CGGCAGGGGATTCCCAATATTCTTTAGATGAAAAGATGCGTAGCCACATG	1090	0.16888175488285184	No Hit
CGGCAGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACT	1065	0.1650083201378323	No Hit
CGGCAGGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	981	0.15199357939456667	No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	968	0.14997939332715648	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	958	0.14843001942914869	No Hit
CGGCAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCT	938	0.14533127163313303	No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	851	0.13185171872046506	No Hit
CGGCAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	850	0.1316967813306643	No Hit
CGGCAGGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	844	0.1307671569918596	No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	802	0.12425978662022677	No Hit
CGGCAGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	771	0.11945672753640253	No Hit
CGGCAGGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCG	768	0.11899191536700018	No Hit
CGGCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	755	0.11697772929959005	No Hit
CGGCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	751	0.11635797974038692	No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	745	0.11542835540158222	No Hit
CGGCAGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGG	729	0.11294935716476971	No Hit
CGGCAGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	706	0.10938579719935175	No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT	701	0.10861111025034784	No Hit
CGGCAGGGGAAGCAAGGAGGCCCGTGAGGAGGGATGAAGAACAAGAACAA	698	0.1081462980809455	No Hit
CGGCAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	692	0.1072166737421408	No Hit
CGGCAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	656	0.10163892770931265	No Hit
CGGCAGGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	651	0.10086424076030877	No Hit
CGGCAGGGGGGCCCCCTCTCCTCTCTGTTCCAGTGACCTGTGTTCCCTCG	647	0.10024449120110564	No Hit
CGGCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	646	0.10008955381130485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.1047376755053283	0.0	0.0	0.0	0.0
6	0.1047376755053283	0.0	0.0	0.0	0.0
7	0.10535742506453143	0.0	0.0	0.0	0.0
8	0.10597717462373454	0.0	0.0	0.0	0.0
9	0.10644198679313689	0.0	0.0	0.0	0.0
10	0.11821722841799628	0.0	0.0	0.0	0.0
11	0.13464059173687912	0.0	0.0	0.0	0.0
12	0.1450213968535315	0.0	0.0	0.0	0.0
13	0.1540077654619768	0.0	0.0	0.0	0.0
14	0.16361388362962526	0.0	0.0	0.0	0.0
15	0.17492431308508233	0.0	0.0	0.0	0.0
16	0.18484030603233234	0.0	0.0	0.0	0.0
17	0.19305198769177376	0.0	0.0	0.0	0.0
18	0.20157354413081674	0.0	0.0	0.0	0.0
19	0.21055991273926206	0.0	0.0	0.0	0.0
20	0.217687032670098	0.0	0.0	0.0	0.0
21	0.22341971609272693	0.0	0.0	0.0	0.0
22	0.22853264995615272	0.0	0.0	0.0	0.0
23	0.2344202707685824	0.0	0.0	0.0	0.0
24	0.2421671402586215	0.0	0.0	0.0	0.0
25	0.25115350886706683	0.0	0.0	0.0	0.0
26	0.2602948148653129	0.0	0.0	0.0	0.0
27	0.26804168435535203	0.0	0.0	0.0	0.0
28	0.2776478025230005	0.0	0.0	0.0	0.0
29	0.28461998506403563	0.0	0.0	0.0	0.0
30	0.2940711658418833	0.0	0.0	0.0	0.0
31	0.3046069083483364	0.0	0.0	0.0	0.0
32	0.31452290129558647	0.0	0.0	0.0	0.0
33	0.32180495861622316	0.0	0.0	0.0	0.0
34	0.3290870159368599	0.0	0.0	0.0	0.0
35	0.34318631840873104	0.0	0.0	0.0	0.0
36	0.35558130959279355	0.0	0.0	0.0	0.0
37	0.36410286603183656	0.0	0.0	0.0	0.0
38	0.37138492335247325	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	7.8539125E-4	44.0	43
AACGTGT	45	4.783942E-10	44.0	35
CTATGCA	45	4.783942E-10	44.0	41
CCTTATT	20	7.8539125E-4	44.0	36
ACTATAG	40	8.301868E-9	44.0	34
GTACCTA	20	7.8539125E-4	44.0	37
AACCGAT	55	1.8189894E-12	44.0	23
ATGATCG	20	7.8539125E-4	44.0	32
CGTATCT	40	8.301868E-9	44.0	22
CGTATCC	20	7.8539125E-4	44.0	13
ATTAGCC	25	4.440665E-5	44.0	34
GTACAGT	20	7.8539125E-4	44.0	13
ATAGGAT	40	8.301868E-9	44.0	10
GGTCGAC	20	7.8539125E-4	44.0	9
CCGATTT	20	7.8539125E-4	44.0	38
CACGGAT	25	4.440665E-5	44.0	23
TTAGCGG	20	7.8539125E-4	44.0	44
CGTGCAC	60	0.0	44.0	24
ATGGATA	40	8.301868E-9	44.0	11
GTTTACG	45	4.783942E-10	44.0	10
>>END_MODULE