FastQCFastQC Report
Fri 17 Jun 2016
SRR1527503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499833
Sequences flagged as poor quality0
Sequence length52
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT28030.5607873029591883No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC26580.5317776137229835No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG26130.5227746067186441No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA23450.4691566983372446No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG17620.3525177409254691No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC15790.31590551244115533No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC14240.28489515498176393No Hit
CGGCAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG10580.2116706980131364No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG10120.2024676241864783No Hit
CGGCAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC10110.20226755736415963No Hit
CGGCAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT10070.201467290074885No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA9870.19746595362851194No Hit
CGGCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT9500.1900634812027217No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9170.1834612760662061No Hit
CGGCAGGGGATTCCCAATATTCTTTAGATGAAAAGATGCGTAGCCACATGGC9160.18326120924388747No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC8560.1712571999047682No Hit
CGGCAGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC8030.1606536583218795No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG7430.14864964898276023No Hit
CGGCAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA7400.1480494485158043No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC7240.14484837935870581No Hit
CGGCAGGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT7170.14344791160247522No Hit
CGGCAGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC7150.1430477779578379No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT6850.1370457732882783No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC6440.12884303357321344No Hit
CGGCAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT6400.12804276628393885No Hit
CGGCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT6320.1264422317053896No Hit
CGGCAGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC6280.125641964416115No Hit
CGGCAGGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTG5980.11963995974655535No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC5700.11403808872163303No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG5680.1136379550769957No Hit
CGGCAGGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC5640.1128376877877211No Hit
CGGCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG5630.11263762096540245No Hit
CGGCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG5570.11143722003149052No Hit
CGGCAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG5460.10923648498598533No Hit
CGGCAGGGGAAGCAAGGAGGCCCGTGAGGAGGGATGAAGAACAAGAACAAAG5320.10643554947352415No Hit
CGGCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC5200.1040347476057003No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA5160.10323448031642568No Hit
CGGCAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC5110.1022341462048324No Hit
CGGCAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG5090.10183401256019511No Hit
CGGCAGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA5040.10083367844860183No Hit
CGGCAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAAC5020.10043354480396452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTCC351.0180156E-746.00000414
ATAATCG351.0180156E-746.00000434
ACTCTAA351.0180156E-746.00000428
TATTCTC351.0180156E-746.00000419
TAGATAT351.0180156E-746.00000415
TCACGCG253.4143657E-546.022
TATTTCG253.4143657E-546.034
CTAGATT206.3080154E-446.012
AACGTGT453.092282E-1046.035
AACGTAC206.3080154E-446.046
ACTATAG301.8592764E-646.034
AACCGAT501.6370905E-1146.023
AATCCAT206.3080154E-446.033
ACACGAA301.8592764E-646.029
CTACTTA253.4143657E-546.021
CATATGG206.3080154E-446.015
CGTATCT453.092282E-1046.022
CGTATCG206.3080154E-446.045
TACTTAT301.8592764E-646.030
AAATAGA253.4143657E-546.021
Produced by FastQC (version 0.11.5)