##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527503_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499833
Sequences flagged as poor quality	0
Sequence length	52
%GC	63
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.069769302947186	33.0	31.0	34.0	31.0	34.0
2	32.245530007022346	34.0	31.0	34.0	31.0	34.0
3	32.796984192720366	34.0	31.0	34.0	31.0	34.0
4	36.37671582308491	37.0	37.0	37.0	35.0	37.0
5	35.61484535834969	37.0	35.0	37.0	35.0	37.0
6	36.09858692803396	37.0	35.0	37.0	35.0	37.0
7	36.53420442427771	37.0	37.0	37.0	35.0	37.0
8	36.63042056046719	37.0	37.0	37.0	35.0	37.0
9	38.616147793362984	39.0	39.0	39.0	39.0	39.0
10	37.45601831011558	39.0	37.0	39.0	34.0	39.0
11	36.26751535012694	37.0	35.0	39.0	33.0	39.0
12	36.07601738980819	38.0	35.0	39.0	31.0	39.0
13	36.248354950553484	39.0	35.0	39.0	32.0	39.0
14	37.05197335910194	39.0	35.0	40.0	33.0	40.0
15	37.2698761386303	39.0	35.0	40.0	33.0	40.0
16	37.43374887212329	39.0	35.0	40.0	33.0	40.0
17	37.34064377502086	39.0	35.0	40.0	33.0	40.0
18	37.242935140336876	39.0	35.0	40.0	33.0	40.0
19	37.10305442017634	39.0	35.0	40.0	33.0	40.0
20	36.98580525895649	39.0	35.0	40.0	33.0	40.0
21	36.944915601811005	39.0	35.0	40.0	33.0	40.0
22	36.854149285861475	39.0	35.0	40.0	32.0	40.0
23	36.614991407129985	38.0	35.0	40.0	32.0	40.0
24	36.498766588040404	38.0	35.0	40.0	31.0	40.0
25	36.48730676045799	38.0	35.0	40.0	32.0	40.0
26	36.45349546748614	38.0	35.0	40.0	32.0	40.0
27	36.40611764329286	38.0	35.0	40.0	32.0	40.0
28	36.24761070197446	38.0	35.0	40.0	31.0	40.0
29	36.125253834780814	38.0	35.0	40.0	31.0	40.0
30	35.95466085672614	38.0	35.0	40.0	31.0	40.0
31	35.81657273529359	38.0	34.0	40.0	31.0	40.0
32	35.69545028039365	37.0	34.0	40.0	30.0	40.0
33	35.371460067662596	37.0	34.0	40.0	30.0	40.0
34	34.92336640437906	36.0	33.0	40.0	28.0	40.0
35	34.72819321653432	36.0	33.0	39.0	27.0	40.0
36	34.55385898890229	35.0	33.0	39.0	27.0	40.0
37	34.33025430493785	35.0	33.0	39.0	26.0	40.0
38	34.52446117003079	35.0	33.0	39.0	27.0	40.0
39	34.41401228010155	35.0	33.0	39.0	27.0	40.0
40	34.25853234980484	35.0	33.0	39.0	27.0	40.0
41	34.03877895217003	35.0	33.0	39.0	26.0	40.0
42	33.84511826950202	35.0	33.0	39.0	26.0	40.0
43	33.78364373700816	35.0	33.0	38.0	26.0	40.0
44	33.61216046159417	35.0	33.0	38.0	25.0	40.0
45	33.522826624092446	35.0	33.0	38.0	25.0	40.0
46	33.36475982978315	35.0	32.0	37.0	25.0	40.0
47	33.157964760229916	35.0	32.0	37.0	24.0	40.0
48	32.97681425596149	35.0	32.0	37.0	23.0	40.0
49	32.72447597497564	34.0	31.0	36.0	24.0	40.0
50	33.361302675093484	35.0	33.0	36.0	26.0	40.0
51	33.53227578011056	35.0	33.0	36.0	27.0	40.0
52	33.22333859509076	35.0	33.0	36.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	6.0
13	8.0
14	15.0
15	19.0
16	54.0
17	107.0
18	169.0
19	306.0
20	505.0
21	709.0
22	1073.0
23	1593.0
24	2187.0
25	2846.0
26	3883.0
27	5104.0
28	6816.0
29	8864.0
30	11972.0
31	16445.0
32	23465.0
33	39321.0
34	65995.0
35	51846.0
36	68819.0
37	85330.0
38	93824.0
39	8549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.75838530069043	1.0259426648500598	0.08522846630774679	0.1304435681517627
8	98.882426730528	0.6760257926147333	0.31950671524289115	0.1220407616143792
9	97.00359920213351	1.2728251235912795	0.8308775130893719	0.8926981611858361
10	65.44645911734519	22.654566625252833	5.242551012038021	6.656423245363952
11	36.60642654646652	25.18501179393918	17.61368297011202	20.594878689482286
12	29.477245399963586	16.334055574561905	28.75260336952542	25.43609565594909
13	23.969005647886394	15.956129347201967	29.15833888518765	30.916526119723986
14	25.81642268517685	16.124785678416593	30.437566147093133	27.621225489313428
15	26.283578715290908	17.36019830623428	26.762738754744085	29.593484223730727
16	30.43916668167168	17.58167227854103	24.119055764625386	27.860105275161906
17	32.42863116280838	18.66443392092959	21.978540832638103	26.928394083623928
18	30.994952314072897	18.857298337644774	22.463102676293882	27.684646671988446
19	30.614225151200504	18.008014676902086	21.89251209904108	29.485248072856336
20	32.188550976025994	19.273037194422937	22.542529204754388	25.995882624796685
21	34.47431442101662	16.12658627981746	23.165937423099315	26.233161876066607
22	33.63903543783624	17.31478313756795	22.8290248943147	26.217156530281116
23	31.446102998401464	18.6988454143684	22.291645409566797	27.563406177663342
24	30.340733805090903	19.498112369531423	21.926523458835252	28.234630366542422
25	29.425428092983058	20.159733350939213	21.60221513985671	28.812623416221015
26	29.84076681611658	19.026754936148674	21.804482697220873	29.32799555051387
27	31.311057893336375	17.823553066724283	21.797880492084357	29.06750854785498
28	32.954006638217166	18.675637662979437	20.513451492798595	27.856904206004806
29	31.20022087377184	19.169002446817238	21.41495259416645	28.215824085244474
30	30.67924686845406	19.020752931479116	20.855565758963493	29.44443444110333
31	31.975879943901266	20.067302479027997	19.899246348280325	28.05757122879042
32	34.90485822264636	19.289442673853067	19.851830511390805	25.953868592109764
33	34.538135737336276	19.146795029539867	20.740327269307947	25.574741963815917
34	32.120328189615336	21.158266861131615	22.4815088239472	24.23989612530585
35	32.47104532913993	22.0739727068841	22.42168884407392	23.033293119902048
36	31.92726370607783	22.07817411015279	20.830357339351345	25.164204844418038
37	31.668577304819813	22.60334951873926	20.13532519861634	25.592747977824594
38	33.77368040925669	21.284709092837005	20.26116722985477	24.68044326805153
39	32.613692973453134	19.678172509618214	21.045429173343898	26.662705343584758
40	31.54353554087065	21.529190749710402	20.255165225185213	26.672108484233735
41	29.88998325440697	19.881840534738604	22.6863772500015	27.541798960852926
42	29.970410116979075	18.18567401512105	22.945863918548795	28.898051949351082
43	28.66377370041594	17.861965896609465	25.051167089807997	28.423093313166596
44	27.437964280069544	18.02361988904294	25.373674807385665	29.16474102350185
45	27.836297323305985	18.702246550347816	24.42635840370684	29.035097722639364
46	29.6865152961089	18.894310699773726	22.48711069497212	28.932063309145256
47	29.419826221958132	20.273971506483164	24.13766197910102	26.16854029245768
48	31.024962337420696	20.173938095323837	22.877841198960454	25.92325836829501
49	29.188949109002404	20.214351593432205	23.719522320455035	26.877176977110356
50	27.746867453729546	20.824355334681783	23.758735417629488	27.670041793959182
51	27.672242529004688	21.732458641186156	22.293045877323024	28.30225295248613
52	29.443634173814054	20.202547650915406	22.085176448933943	28.268641726336597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.0
11	2.5
12	2.0
13	5.5
14	40.5
15	72.0
16	118.0
17	164.0
18	265.0
19	366.0
20	414.5
21	463.0
22	419.5
23	376.0
24	359.5
25	343.0
26	416.0
27	489.0
28	567.5
29	646.0
30	769.5
31	893.0
32	1056.5
33	1220.0
34	1495.0
35	1770.0
36	1862.5
37	1955.0
38	2436.0
39	3510.5
40	4104.0
41	4675.5
42	5247.0
43	5456.0
44	5665.0
45	6307.5
46	6950.0
47	7461.0
48	7972.0
49	9305.0
50	10638.0
51	11460.0
52	12282.0
53	14323.0
54	16364.0
55	17266.5
56	18169.0
57	22497.0
58	26825.0
59	27947.5
60	29070.0
61	34601.5
62	40133.0
63	44562.5
64	47217.0
65	45442.0
66	47469.5
67	49497.0
68	46072.0
69	42647.0
70	40118.0
71	37589.0
72	30583.5
73	23578.0
74	19851.0
75	16124.0
76	15515.5
77	14907.0
78	15127.0
79	15347.0
80	11210.0
81	7073.0
82	4614.0
83	2155.0
84	1510.5
85	866.0
86	597.5
87	329.0
88	219.5
89	83.0
90	56.0
91	33.5
92	11.0
93	6.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	499833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.908843553746951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.26468106958148	7.446887260344955
2	9.452371345779994	2.0622888044606897
3	3.358031031067747	1.0989670549963688
4	1.5735612368411402	0.686629334197622
5	0.9023218281186957	0.4921643829038899
6	0.6235557348787734	0.408136317530055
7	0.46950078861460587	0.35851974559502875
8	0.3539595789164802	0.3089031736600024
9	0.3796354032938415	0.37272448997965324
>10	10.218978102189782	30.923928592149778
>50	3.081098925283351	22.77400651817707
>100	1.2471114697575467	25.238429635498257
>500	0.05501962366577412	3.9727268907815207
>1k	0.020173862010783843	3.8556877997251084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2803	0.5607873029591883	No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2658	0.5317776137229835	No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2613	0.5227746067186441	No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2345	0.4691566983372446	No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1762	0.3525177409254691	No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1579	0.31590551244115533	No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1424	0.28489515498176393	No Hit
CGGCAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG	1058	0.2116706980131364	No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1012	0.2024676241864783	No Hit
CGGCAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC	1011	0.20226755736415963	No Hit
CGGCAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1007	0.201467290074885	No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	987	0.19746595362851194	No Hit
CGGCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	950	0.1900634812027217	No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	917	0.1834612760662061	No Hit
CGGCAGGGGATTCCCAATATTCTTTAGATGAAAAGATGCGTAGCCACATGGC	916	0.18326120924388747	No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	856	0.1712571999047682	No Hit
CGGCAGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC	803	0.1606536583218795	No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	743	0.14864964898276023	No Hit
CGGCAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	740	0.1480494485158043	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	724	0.14484837935870581	No Hit
CGGCAGGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	717	0.14344791160247522	No Hit
CGGCAGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC	715	0.1430477779578379	No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	685	0.1370457732882783	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	644	0.12884303357321344	No Hit
CGGCAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT	640	0.12804276628393885	No Hit
CGGCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	632	0.1264422317053896	No Hit
CGGCAGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	628	0.125641964416115	No Hit
CGGCAGGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTG	598	0.11963995974655535	No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC	570	0.11403808872163303	No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	568	0.1136379550769957	No Hit
CGGCAGGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	564	0.1128376877877211	No Hit
CGGCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	563	0.11263762096540245	No Hit
CGGCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	557	0.11143722003149052	No Hit
CGGCAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	546	0.10923648498598533	No Hit
CGGCAGGGGAAGCAAGGAGGCCCGTGAGGAGGGATGAAGAACAAGAACAAAG	532	0.10643554947352415	No Hit
CGGCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	520	0.1040347476057003	No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	516	0.10323448031642568	No Hit
CGGCAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	511	0.1022341462048324	No Hit
CGGCAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG	509	0.10183401256019511	No Hit
CGGCAGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	504	0.10083367844860183	No Hit
CGGCAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAAC	502	0.10043354480396452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.1132378214323584	0.0	0.0	0.0	0.0
6	0.1132378214323584	0.0	0.0	0.0	0.0
7	0.11363795507699571	0.0	0.0	0.0	0.0
8	0.11363795507699571	0.0	0.0	0.0	0.0
9	0.11423815554395168	0.0	0.0	0.0	0.0
10	0.1290431003955321	0.0	0.0	0.0	0.0
11	0.14544857982566178	0.0	0.0	0.0	0.0
12	0.15565198776391315	0.0	0.0	0.0	0.0
13	0.16525519523520857	0.0	0.0	0.0	0.0
14	0.1796600064421517	0.0	0.0	0.0	0.0
15	0.19046361484735902	0.0	0.0	0.0	0.0
16	0.2010671564302477	0.0	0.0	0.0	0.0
17	0.2102702302569058	0.0	0.0	0.0	0.0
18	0.21767270268269603	0.0	0.0	0.0	0.0
19	0.22507517510848624	0.0	0.0	0.0	0.0
20	0.22927657837717796	0.0	0.0	0.0	0.0
21	0.23687911762528685	0.0	0.0	0.0	0.0
22	0.24088045407165992	0.0	0.0	0.0	0.0
23	0.2484829933197688	0.0	0.0	0.0	0.0
24	0.25408486434469113	0.0	0.0	0.0	0.0
25	0.26308787134903056	0.0	0.0	0.0	0.0
26	0.27249101199800735	0.0	0.0	0.0	0.0
27	0.27969341760147887	0.0	0.0	0.0	0.0
28	0.29209756058523545	0.0	0.0	0.0	0.0
29	0.30190083487884956	0.0	0.0	0.0	0.0
30	0.3117041091724636	0.0	0.0	0.0	0.0
31	0.3223076507553523	0.0	0.0	0.0	0.0
32	0.3335113928051969	0.0	0.0	0.0	0.0
33	0.3397134642970752	0.0	0.0	0.0	0.0
34	0.34811627083445873	0.0	0.0	0.0	0.0
35	0.35811961195039144	0.0	0.0	0.0	0.0
36	0.370523754934148	0.0	0.0	0.0	0.0
37	0.37912662829385013	0.0	0.0	0.0	0.0
38	0.3891299694097829	0.0	0.0	0.0	0.0
39	0.39893324370339694	0.0	0.0	0.0	0.0
40	0.408736517997011	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTCC	35	1.0180156E-7	46.000004	14
ATAATCG	35	1.0180156E-7	46.000004	34
ACTCTAA	35	1.0180156E-7	46.000004	28
TATTCTC	35	1.0180156E-7	46.000004	19
TAGATAT	35	1.0180156E-7	46.000004	15
TCACGCG	25	3.4143657E-5	46.0	22
TATTTCG	25	3.4143657E-5	46.0	34
CTAGATT	20	6.3080154E-4	46.0	12
AACGTGT	45	3.092282E-10	46.0	35
AACGTAC	20	6.3080154E-4	46.0	46
ACTATAG	30	1.8592764E-6	46.0	34
AACCGAT	50	1.6370905E-11	46.0	23
AATCCAT	20	6.3080154E-4	46.0	33
ACACGAA	30	1.8592764E-6	46.0	29
CTACTTA	25	3.4143657E-5	46.0	21
CATATGG	20	6.3080154E-4	46.0	15
CGTATCT	45	3.092282E-10	46.0	22
CGTATCG	20	6.3080154E-4	46.0	45
TACTTAT	30	1.8592764E-6	46.0	30
AAATAGA	25	3.4143657E-5	46.0	21
>>END_MODULE