FastQCFastQC Report
Fri 17 Jun 2016
SRR1527502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683313
Sequences flagged as poor quality0
Sequence length52
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT40000.5853832723803001No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG37620.5505529676736722No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC36140.5288937865956012No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA29230.4277688262919043No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG23800.34830304706627857No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC21300.3117165925425098No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC20860.30527737654632653No Hit
CGGCAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG15030.21995776459689778No Hit
CGGCAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT14200.20781106169500654No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG13730.20093280824453802No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG13370.1956643587931153No Hit
CGGCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT12880.18849341370645664No Hit
CGGCAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC12840.18790803043407633No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC12500.18293227261884376No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA12450.18220054352836842No Hit
CGGCAGGGGATTCCCAATATTCTTTAGATGAAAAGATGCGTAGCCACATGGC12150.17781016898551616No Hit
CGGCAGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC11630.17020018644457224No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC10610.1552729129988746No Hit
CGGCAGGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT10300.15073619263792729No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10010.1464921639131701No Hit
CGGCAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT10000.14634581809507502No Hit
CGGCAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA10000.14634581809507502No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT9690.1418090977341277No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC9530.1394675646446065No Hit
CGGCAGGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC9110.13332104028461333No Hit
CGGCAGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC8670.12688182428843003No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA8340.12205241229129257No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC8190.11985722501986645No Hit
CGGCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT8000.11707665447606003No Hit
CGGCAGGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTG7970.11663761702177479No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG7950.11634492538558465No Hit
CGGCAGGGGAAGCAAGGAGGCCCGTGAGGAGGGATGAAGAACAAGAACAAAG7600.111222821752257No Hit
CGGCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG7580.11093013011606687No Hit
CGGCAGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC7560.11063743847987671No Hit
CGGCAGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA7430.10873494284464075No Hit
CGGCAGGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG7330.10727148466368999No Hit
CGGCAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG7300.10683244720940477No Hit
CGGCAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC7180.10507629739226387No Hit
CGGCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG7150.10463725993797865No Hit
CGGCAGGGGGGCCCCCTCTCCTCTCTGTTCCAGTGACCTGTGTTCCCTCGCT7090.10375918502940819No Hit
CGGCAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAAC7030.10288111012083773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGTA253.4156918E-546.026
AACGTGT206.309649E-446.035
TATCAAT253.4156918E-546.028
AACGTAT206.309649E-446.035
ACTATAG453.092282E-1046.034
TGATACG206.309649E-446.010
AACCGAT453.092282E-1046.023
GATACGC206.309649E-446.011
AAATCGT501.6370905E-1146.018
CGAACGA253.4156918E-546.015
CGAACAA453.092282E-1046.032
CGTATTA206.309649E-446.037
CTACTTA650.046.021
CGTATCT501.6370905E-1146.022
ATTAGCG206.309649E-446.027
GTATTAT206.309649E-446.010
ATAGGTC253.4156918E-546.046
GGTCGAC301.8602859E-646.09
ACGTTTA206.309649E-446.028
TAAAGCG206.309649E-446.041
Produced by FastQC (version 0.11.5)