##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683313
Sequences flagged as poor quality	0
Sequence length	52
%GC	63
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02561198162482	31.0	31.0	33.0	30.0	34.0
2	31.302814376427786	31.0	31.0	34.0	28.0	34.0
3	32.023286546575285	34.0	31.0	34.0	30.0	34.0
4	35.871531787043416	37.0	35.0	37.0	35.0	37.0
5	34.683180328780516	35.0	35.0	37.0	32.0	37.0
6	35.42806737176082	37.0	35.0	37.0	32.0	37.0
7	36.26862945677896	37.0	35.0	37.0	35.0	37.0
8	36.42870543952771	37.0	37.0	37.0	35.0	37.0
9	38.48290022288468	39.0	39.0	39.0	37.0	39.0
10	36.88604197490755	39.0	37.0	39.0	32.0	39.0
11	35.4377642456678	37.0	35.0	39.0	31.0	39.0
12	35.229629759714804	37.0	35.0	39.0	27.0	39.0
13	35.318539234582104	37.0	35.0	39.0	30.0	39.0
14	36.201008908069944	38.0	35.0	40.0	30.0	40.0
15	36.468223200787925	38.0	35.0	40.0	31.0	40.0
16	36.58959656848326	38.0	35.0	40.0	32.0	40.0
17	36.53963996001832	38.0	35.0	40.0	32.0	40.0
18	36.493805913249126	38.0	35.0	40.0	31.0	40.0
19	36.29555269693391	38.0	35.0	40.0	31.0	40.0
20	36.136419181253686	38.0	35.0	40.0	30.0	40.0
21	36.171878772978125	38.0	35.0	40.0	31.0	40.0
22	36.0840698186629	38.0	35.0	40.0	30.0	40.0
23	35.900433622659015	38.0	34.0	40.0	30.0	40.0
24	35.82445233736223	38.0	34.0	40.0	30.0	40.0
25	35.81307395000534	38.0	34.0	40.0	30.0	40.0
26	35.70537074517827	38.0	34.0	40.0	30.0	40.0
27	35.60696049980024	37.0	34.0	40.0	30.0	40.0
28	35.37725610371821	37.0	34.0	40.0	29.0	40.0
29	35.31531816312583	37.0	34.0	40.0	29.0	40.0
30	35.20025815402312	37.0	34.0	40.0	29.0	40.0
31	35.0386894439298	37.0	33.0	40.0	28.0	40.0
32	34.94102263530768	36.0	33.0	39.0	28.0	40.0
33	34.6657651764272	36.0	33.0	39.0	27.0	40.0
34	34.336418303178775	35.0	33.0	39.0	26.0	40.0
35	34.16775328436602	35.0	33.0	39.0	26.0	40.0
36	33.976439786744876	35.0	33.0	39.0	26.0	40.0
37	33.8369005126494	35.0	32.0	39.0	26.0	40.0
38	33.777661188942695	35.0	32.0	38.0	25.0	40.0
39	33.55407112114068	35.0	32.0	38.0	25.0	40.0
40	33.969911299799655	35.0	33.0	39.0	26.0	40.0
41	34.04931854069804	35.0	33.0	39.0	26.0	40.0
42	34.098432197250744	35.0	33.0	39.0	27.0	40.0
43	34.03612253828041	35.0	33.0	38.0	27.0	40.0
44	33.917591206372485	35.0	33.0	38.0	27.0	40.0
45	33.88399020653785	35.0	33.0	38.0	27.0	40.0
46	33.6379009326619	35.0	33.0	37.0	26.0	40.0
47	33.52136868462915	35.0	33.0	37.0	26.0	40.0
48	33.43245920976185	35.0	33.0	37.0	26.0	40.0
49	33.23804464425527	35.0	32.0	36.0	26.0	40.0
50	33.13807435245634	35.0	32.0	36.0	26.0	40.0
51	32.95867925826086	35.0	32.0	36.0	25.0	39.0
52	32.521863333494316	34.0	32.0	35.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	12.0
14	26.0
15	42.0
16	95.0
17	174.0
18	321.0
19	611.0
20	969.0
21	1504.0
22	2131.0
23	3009.0
24	4201.0
25	5614.0
26	7474.0
27	9830.0
28	12699.0
29	16492.0
30	21269.0
31	28346.0
32	38393.0
33	58100.0
34	89286.0
35	71741.0
36	95546.0
37	109365.0
38	99837.0
39	6220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.897869643926	0.909100222006606	0.08239269558752724	0.11063743847987671
8	98.98392098496589	0.6270918305373965	0.2741057172920755	0.1148814672046339
9	97.18137954348886	1.2108652989186508	0.7652422828191473	0.8425128747733469
10	65.65380725963065	22.71052943526612	5.000636604308713	6.635026700794512
11	36.357569664268055	25.354998368244132	17.657061990625085	20.630369976862724
12	29.23038197721981	16.382975298289367	28.852224383262136	25.534418341228694
13	23.77680506590684	15.875008963681358	29.236821193215995	31.11136477719581
14	25.57744401174864	16.104918243908724	30.495541574651735	27.8220961696909
15	26.26161071134312	17.246269279232212	26.743381144512107	29.748738864912568
16	30.31919486384717	17.64959835390224	24.09027780826649	27.940928973984104
17	32.11807765987183	18.892513386983712	21.980410148789794	27.00899880435467
18	30.84808865044277	18.91870928842273	22.40130072163123	27.831901339503272
19	30.542372236442155	17.92648464173812	22.044509617115434	29.486633504704287
20	31.90353469054445	19.355112517982242	22.53681694918727	26.204535842286035
21	34.63024997329189	15.98345121488981	23.234008426592208	26.152290385226095
22	33.64461088842155	17.184364998177994	22.822337640290762	26.34868647310969
23	31.18673287351477	18.743826035799113	22.33017665403702	27.73926443664909
24	30.097919986887415	19.480530884089724	21.920847400825096	28.500701728197765
25	29.360629755324425	20.12386710043567	21.59932563847022	28.916177505769685
26	29.79044742306966	18.892074349529427	21.754159514014805	29.563318713386106
27	31.154536793533854	17.947119402089527	21.787233668904292	29.111110135472323
28	33.09508234147455	18.820072207026648	20.429144476981996	27.6557009745168
29	31.10141326156534	19.183156181720527	21.404832046221863	28.310598510492262
30	30.502712519738388	18.904074706613223	20.8272051022006	29.766007671447785
31	31.89768085782065	20.125476904434716	19.857225019866444	28.11961721787819
32	34.89645301640683	19.29247650783755	19.836443913696943	25.974626562058678
33	34.44892750467209	19.085250829414925	20.676761601198866	25.78906006471412
34	31.918169272353957	21.223216885965876	22.51003566447587	24.348578177204296
35	32.48906430874284	22.09734045744776	22.43905794269976	22.974537291109637
36	31.859923636752118	22.08870605418015	20.73749511570832	25.313875193359415
37	31.543084940576282	22.6427713214881	20.161770667322294	25.652373070613322
38	33.92325332607458	21.259510648853453	20.02713251467483	24.790103510397138
39	32.54891974834373	19.64092590072192	20.891304570526245	26.9188497804081
40	31.40493448829453	21.642790346444453	20.145233589877552	26.80704157538346
41	29.889523541920028	19.87200594749405	22.692675245458524	27.545795265127403
42	29.706883960937375	18.14863759360644	22.83785029700884	29.306628148447345
43	28.609436671042403	17.869556118499137	25.133138108011995	28.387869102446462
44	27.412474224842786	18.00697484169041	25.348266460611757	29.232284472855046
45	27.92556266308412	18.699775944552496	24.327358033580513	29.047303358782873
46	29.808301612877266	18.890171853894188	22.539304828094885	28.762221705133665
47	29.352580735329198	20.313677626504983	23.968810779247576	26.36493085891824
48	31.106681711016765	20.000351229963428	22.95580502639347	25.937162032626336
49	29.04788874205525	20.194405784757496	23.80344000480014	26.954265468387106
50	27.791509893709033	20.80232631312444	23.696753903408833	27.709409889757698
51	27.739410782467182	21.61352118282544	22.15222013923341	28.494847895473963
52	29.346726902605397	20.188844643669885	22.079486267640156	28.38494218608456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.5
10	5.0
11	4.0
12	3.0
13	7.0
14	53.0
15	95.0
16	152.5
17	210.0
18	340.5
19	471.0
20	514.5
21	558.0
22	533.5
23	509.0
24	470.5
25	432.0
26	516.5
27	601.0
28	756.5
29	912.0
30	1077.5
31	1243.0
32	1485.5
33	1728.0
34	2157.5
35	2587.0
36	2596.5
37	2606.0
38	3283.5
39	4754.5
40	5548.0
41	6076.0
42	6604.0
43	7075.0
44	7546.0
45	8479.5
46	9413.0
47	10229.5
48	11046.0
49	12701.5
50	14357.0
51	15609.0
52	16861.0
53	19941.0
54	23021.0
55	23754.0
56	24487.0
57	30237.0
58	35987.0
59	37760.5
60	39534.0
61	47396.5
62	55259.0
63	61378.5
64	64985.0
65	62472.0
66	65091.0
67	67710.0
68	63122.0
69	58534.0
70	55465.5
71	52397.0
72	42228.5
73	32060.0
74	26797.0
75	21534.0
76	20989.5
77	20445.0
78	20641.5
79	20838.0
80	15240.0
81	9642.0
82	6296.0
83	2950.0
84	2005.0
85	1060.0
86	718.5
87	377.0
88	251.5
89	93.5
90	61.0
91	36.5
92	12.0
93	7.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	683313.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.38689443929795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.06410707995772	7.277484842231891
2	10.081014441704825	2.094208656940524
3	3.732300105671011	1.1630102164015612
4	1.7710461430081013	0.7358267733820373
5	0.9468122578372666	0.49172194879945214
6	0.5551250440295878	0.34596151397675734
7	0.4043677351179993	0.29400874855300574
8	0.3127861923212399	0.25991017293685326
9	0.25361042620641067	0.23708022531402154
>10	6.541740049313138	19.69009809560187
>50	3.6238112011271575	26.136338691053734
>100	1.6047904191616766	30.36734263800045
>500	0.08030996829869673	5.48269972911389
>1k	0.028178936245156747	5.424307747693955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4000	0.5853832723803001	No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3762	0.5505529676736722	No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3614	0.5288937865956012	No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2923	0.4277688262919043	No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2380	0.34830304706627857	No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2130	0.3117165925425098	No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2086	0.30527737654632653	No Hit
CGGCAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG	1503	0.21995776459689778	No Hit
CGGCAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1420	0.20781106169500654	No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1373	0.20093280824453802	No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1337	0.1956643587931153	No Hit
CGGCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1288	0.18849341370645664	No Hit
CGGCAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC	1284	0.18790803043407633	No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1250	0.18293227261884376	No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1245	0.18220054352836842	No Hit
CGGCAGGGGATTCCCAATATTCTTTAGATGAAAAGATGCGTAGCCACATGGC	1215	0.17781016898551616	No Hit
CGGCAGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC	1163	0.17020018644457224	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1061	0.1552729129988746	No Hit
CGGCAGGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1030	0.15073619263792729	No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1001	0.1464921639131701	No Hit
CGGCAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT	1000	0.14634581809507502	No Hit
CGGCAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1000	0.14634581809507502	No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	969	0.1418090977341277	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	953	0.1394675646446065	No Hit
CGGCAGGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	911	0.13332104028461333	No Hit
CGGCAGGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC	867	0.12688182428843003	No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	834	0.12205241229129257	No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC	819	0.11985722501986645	No Hit
CGGCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	800	0.11707665447606003	No Hit
CGGCAGGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTG	797	0.11663761702177479	No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	795	0.11634492538558465	No Hit
CGGCAGGGGAAGCAAGGAGGCCCGTGAGGAGGGATGAAGAACAAGAACAAAG	760	0.111222821752257	No Hit
CGGCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	758	0.11093013011606687	No Hit
CGGCAGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	756	0.11063743847987671	No Hit
CGGCAGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	743	0.10873494284464075	No Hit
CGGCAGGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	733	0.10727148466368999	No Hit
CGGCAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	730	0.10683244720940477	No Hit
CGGCAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	718	0.10507629739226387	No Hit
CGGCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	715	0.10463725993797865	No Hit
CGGCAGGGGGGCCCCCTCTCCTCTCTGTTCCAGTGACCTGTGTTCCCTCGCT	709	0.10375918502940819	No Hit
CGGCAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAAC	703	0.10288111012083773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0938076693989431	0.0	0.0	0.0	0.0
6	0.0938076693989431	0.0	0.0	0.0	0.0
7	0.09410036103513324	0.0	0.0	0.0	0.0
8	0.09483209012560861	0.0	0.0	0.0	0.0
9	0.09541747339798892	0.0	0.0	0.0	0.0
10	0.10727148466368999	0.0	0.0	0.0	0.0
11	0.12234510392748273	0.0	0.0	0.0	0.0
12	0.1312721988312823	0.0	0.0	0.0	0.0
13	0.13961391046270158	0.0	0.0	0.0	0.0
14	0.15044350100173712	0.0	0.0	0.0	0.0
15	0.15907790426934657	0.0	0.0	0.0	0.0
16	0.16741961590076582	0.0	0.0	0.0	0.0
17	0.17517594425980482	0.0	0.0	0.0	0.0
18	0.18132246861979795	0.0	0.0	0.0	0.0
19	0.18820072207026647	0.0	0.0	0.0	0.0
20	0.1939082089759744	0.0	0.0	0.0	0.0
21	0.19859127515501682	0.0	0.0	0.0	0.0
22	0.20503049115120012	0.0	0.0	0.0	0.0
23	0.21059163223881297	0.0	0.0	0.0	0.0
24	0.21820161477975686	0.0	0.0	0.0	0.0
25	0.2278604387740318	0.0	0.0	0.0	0.0
26	0.23488503804259542	0.0	0.0	0.0	0.0
27	0.24117790822068363	0.0	0.0	0.0	0.0
28	0.2512757696692438	0.0	0.0	0.0	0.0
29	0.25815402311971236	0.0	0.0	0.0	0.0
30	0.2673738096597021	0.0	0.0	0.0	0.0
31	0.2765935961996918	0.0	0.0	0.0	0.0
32	0.2850816536492061	0.0	0.0	0.0	0.0
33	0.29225259873586484	0.0	0.0	0.0	0.0
34	0.2992771980044284	0.0	0.0	0.0	0.0
35	0.3144971630863162	0.0	0.0	0.0	0.0
36	0.3237169496263059	0.0	0.0	0.0	0.0
37	0.3323513528939154	0.0	0.0	0.0	0.0
38	0.33893691470819376	0.0	0.0	0.0	0.0
39	0.3474249721577081	0.0	0.0	0.0	0.0
40	0.3543032256081766	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGTA	25	3.4156918E-5	46.0	26
AACGTGT	20	6.309649E-4	46.0	35
TATCAAT	25	3.4156918E-5	46.0	28
AACGTAT	20	6.309649E-4	46.0	35
ACTATAG	45	3.092282E-10	46.0	34
TGATACG	20	6.309649E-4	46.0	10
AACCGAT	45	3.092282E-10	46.0	23
GATACGC	20	6.309649E-4	46.0	11
AAATCGT	50	1.6370905E-11	46.0	18
CGAACGA	25	3.4156918E-5	46.0	15
CGAACAA	45	3.092282E-10	46.0	32
CGTATTA	20	6.309649E-4	46.0	37
CTACTTA	65	0.0	46.0	21
CGTATCT	50	1.6370905E-11	46.0	22
ATTAGCG	20	6.309649E-4	46.0	27
GTATTAT	20	6.309649E-4	46.0	10
ATAGGTC	25	3.4156918E-5	46.0	46
GGTCGAC	30	1.8602859E-6	46.0	9
ACGTTTA	20	6.309649E-4	46.0	28
TAAAGCG	20	6.309649E-4	46.0	41
>>END_MODULE