FastQCFastQC Report
Fri 17 Jun 2016
SRR1527501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374403
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGA29760.7948654257578064No Hit
ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT29220.7804424644033301No Hit
ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20660.5518118177471869No Hit
ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC16020.42788118684946436No Hit
ACTTATGGGGAAGATCAAGCCAAAGAATGCAAAACAGCCAGAACGTGAAG10710.2860554001971138No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG8770.23423957607177295No Hit
ACTTATGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT7850.20966712339377624No Hit
ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG7560.20192145896266855No Hit
ACTTATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG7490.20005181582412537No Hit
ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC7310.1952441620392999No Hit
ACTTATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT7290.1947099782854304No Hit
ACTTATGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTC7060.18856686511593124No Hit
ACTTATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT6990.186697221977388No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC6630.17708191440773713No Hit
ACTTATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT6460.17254135249984642No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT6130.16372732056099978No Hit
ACTTATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTC5970.15945385053004385No Hit
ACTTATGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT5970.15945385053004385No Hit
ACTTATGGGGGTCCCAAGATGGCGTCGTGGCTGCCGGAGACTCTGTTTGA5770.1541120129913489No Hit
ACTTATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC5700.15224236985280568No Hit
ACTTATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA5680.1517081860989362No Hit
ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA5590.14930435920652346No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC5590.14930435920652346No Hit
ACTTATGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG5450.14556507292943702No Hit
ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA5450.14556507292943702No Hit
ACTTATGGGAACCCTTCTCCACATGGCTATCGAGCCCATCCTAGCCGACG5430.1450308891755675No Hit
ACTTATGGGGTCGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCG5400.14422961354476324No Hit
ACTTATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA5250.14022323539074205No Hit
ACTTATGGGATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGA5170.13808650037526407No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5160.13781940849832933No Hit
ACTTATGGGGGCGCTTCCGGTCCCTTCTCCCTCTGGCCGACGTGATTCCC5000.1335459384673734No Hit
ACTTATGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC4990.13327884659043865No Hit
ACTTATGGGGAGCGTCCCTTGTGTCGCCTGCCTCTGAAGTGGCATGGTCA4900.13087501969802592No Hit
ACTTATGGGGGCGGTACCCGCGGCAGAGTTAATCAGTTGGTCACTGCACC4890.13060792782109118No Hit
ACTTATGGGGGATCCAGGATAACGGAGGCTGGGATGCCTTTGTGGAACTA4760.12713573342093945No Hit
ACTTATGGGCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTTACG4760.12713573342093945No Hit
ACTTATGGGAGCCCAGCCCTGGGATTGGGGACCCGAGTGCAATTGCTCCT4710.12580027403626573No Hit
ACTTATGGGGGAAGCTTTGGTGGTGGTGTCTTGACACTTGGGTCATGTTC4660.124464814651592No Hit
ACTTATGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCA4640.12393063089772251No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG4570.12206098775917927No Hit
ACTTATGGGGAGAAGGAGAAGAAAGTGGGGCTGTATACAATCTATGTGAA4490.1199242527437013No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG4450.11885588523596231No Hit
ACTTATGGGGGCGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAG4340.1159178745896801No Hit
ACTTATGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGA4310.11511659895887585No Hit
ACTTATGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTG4290.11458241520500637No Hit
ACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCT4240.11324695582033263No Hit
ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA4220.11271277206646314No Hit
ACTTATGGGGTGTGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGG4090.10924057766631143No Hit
ACTTATGGGGGCACCATGGACTCGGTGCGTTCAGGGCCCTTCGGGCAGAT4090.10924057766631143No Hit
ACTTATGGGGGTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCC4080.10897348578937668No Hit
ACTTATGGGGCCAAAGCGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTG4060.1084393020355072No Hit
ACTTATGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA3990.10656965889696396No Hit
ACTTATGGGAGTTCAGGTTTGCCAGTAGAAAGCCTGTATCGTGGAGGACA3910.10443292388148599No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC3890.10389874012761649No Hit
ACTTATGGGCATTAGAAAGTAAAACTCAGCCGGGCGTGGTGGCACATGCC3880.10363164825068175No Hit
ACTTATGGGAGATCCGGAAGAAGATGATGGAAATCATGACCCGAGAGGTG3780.10096072948133428No Hit
ACTTATGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3770.10069363760439953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACA750.044.00000433
TCCGACC650.044.00000423
ATAATCT750.044.00000440
ACGGCCA750.044.00000411
CTTACAA750.044.00000434
GTGTTAT207.8496744E-444.013
ACTATTT207.8496744E-444.010
AACGTAC254.4370732E-544.033
AGCGTGC454.783942E-1044.016
TAATAGA254.4370732E-544.021
AACAACG207.8496744E-444.044
AACCGAC207.8496744E-444.010
GATACCG551.8189894E-1244.042
TCGTTAC302.5231275E-644.039
GGTACGC207.8496744E-444.09
ACAGTCG254.4370732E-544.010
GTACCCC254.4370732E-544.030
CAAACGT254.4370732E-544.017
TACCCCA207.8496744E-444.013
AAATCAC302.5231275E-644.010
Produced by FastQC (version 0.11.5)