##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527501_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 374403 Sequences flagged as poor quality 0 Sequence length 50 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.870468452442957 31.0 30.0 33.0 25.0 33.0 2 31.305486334244115 31.0 31.0 33.0 30.0 34.0 3 30.95505644986819 31.0 31.0 31.0 30.0 34.0 4 35.48739726978683 35.0 35.0 37.0 35.0 37.0 5 34.906443057347296 35.0 35.0 37.0 33.0 37.0 6 35.73687977927527 36.0 35.0 37.0 35.0 37.0 7 36.445672176772085 37.0 36.0 37.0 35.0 37.0 8 36.646867145829496 37.0 37.0 37.0 35.0 37.0 9 38.58846750693771 39.0 39.0 39.0 38.0 39.0 10 37.181528994158704 39.0 37.0 39.0 34.0 39.0 11 37.22296295702759 39.0 37.0 39.0 34.0 39.0 12 37.179477728543844 39.0 37.0 39.0 34.0 39.0 13 37.15324129347254 39.0 37.0 39.0 34.0 39.0 14 38.01344807600366 40.0 38.0 40.0 34.0 40.0 15 38.04650069577434 40.0 38.0 40.0 34.0 40.0 16 38.099609244584045 40.0 38.0 40.0 34.0 40.0 17 38.03866956194261 40.0 38.0 40.0 34.0 40.0 18 37.82946183657717 40.0 37.0 40.0 34.0 40.0 19 37.74499135957778 40.0 37.0 40.0 34.0 40.0 20 37.60576704780677 39.0 37.0 40.0 33.0 40.0 21 37.55904199485581 39.0 37.0 40.0 33.0 40.0 22 37.37755306447865 39.0 36.0 40.0 33.0 40.0 23 37.16480370082505 38.0 36.0 40.0 33.0 40.0 24 36.983624597025134 38.0 36.0 40.0 33.0 40.0 25 36.76460925793864 38.0 36.0 40.0 32.0 40.0 26 36.02877380790218 38.0 35.0 39.0 31.0 40.0 27 35.91058298143979 38.0 35.0 39.0 31.0 40.0 28 35.73278526080186 37.0 34.0 39.0 31.0 40.0 29 35.311712245895464 37.0 34.0 38.0 30.0 40.0 30 34.98130623953334 36.0 34.0 38.0 30.0 40.0 31 34.77794782627276 36.0 33.0 38.0 29.0 40.0 32 34.51090402587586 36.0 33.0 38.0 29.0 40.0 33 34.102314885297396 36.0 33.0 38.0 27.0 39.0 34 33.49556761030227 35.0 32.0 38.0 26.0 39.0 35 33.46726922594103 35.0 32.0 38.0 26.0 39.0 36 33.02054203625505 35.0 31.0 38.0 25.0 39.0 37 32.48429366217685 34.0 31.0 37.0 24.0 38.0 38 31.89447467034185 34.0 30.0 37.0 23.0 38.0 39 31.153081038346382 33.0 29.0 36.0 21.0 38.0 40 30.831296757771707 33.0 29.0 36.0 20.0 38.0 41 30.517397029404144 33.0 28.0 36.0 19.0 38.0 42 30.18958715608582 32.0 28.0 35.0 19.0 38.0 43 29.70058466411861 32.0 27.0 35.0 18.0 37.0 44 29.26134673066188 31.0 26.0 34.0 18.0 37.0 45 28.8841275310294 31.0 26.0 34.0 18.0 37.0 46 28.559106096906273 31.0 26.0 34.0 18.0 37.0 47 28.065977035440422 30.0 24.0 33.0 16.0 36.0 48 27.40146847113939 30.0 24.0 33.0 14.0 35.0 49 26.770311669511194 29.0 23.0 33.0 13.0 35.0 50 30.134518152899417 33.0 28.0 35.0 20.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 4.0 15 11.0 16 28.0 17 57.0 18 121.0 19 172.0 20 376.0 21 588.0 22 882.0 23 1335.0 24 2052.0 25 3047.0 26 4426.0 27 6070.0 28 8199.0 29 11067.0 30 13182.0 31 15936.0 32 21941.0 33 32485.0 34 51199.0 35 95536.0 36 91463.0 37 13899.0 38 325.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.5595654949346 0.2473270780415755 0.11752042585128858 0.07558700117253335 8 99.23638432384357 0.5144189549763223 0.08760613563459695 0.1615905855455218 9 97.45434732093493 1.486366295141866 0.4399003213115279 0.6193860626116778 10 55.18225014222643 29.62850190837146 5.310053605339701 9.879194344062414 11 29.22893246047708 28.742825244455837 19.691882810768078 22.336359484299003 12 28.090052697227318 19.79578155089569 27.4941707197859 24.619995032091087 13 22.066062504841042 18.97126892679813 28.561736951894083 30.400931616466746 14 23.62454360675529 23.00061698223572 29.948477976939287 23.426361434069705 15 26.115709542925668 20.097061188078087 27.732950857765566 26.05427841123068 16 27.80533275641488 20.540967887543637 26.730020859875587 24.923678496165895 17 32.05556579407751 21.258109577113434 20.35213393055077 26.334190698258293 18 29.956757825124264 21.861737218985958 22.63203019206577 25.54947476382401 19 28.691276512207438 21.160353950155315 23.625879066139962 26.52249047149729 20 25.150172407806558 25.45011658560428 22.215901047801434 27.183809958787723 21 29.47652663039559 21.196411353541507 24.757013164958614 24.570048851104293 22 28.958101297265248 21.43919786967519 22.30564391845151 27.297056914608053 23 28.410562949549018 26.942091810161777 20.589311517268825 24.05803372302038 24 27.994166713407747 25.59114109662583 21.124296546769123 25.290395643197307 25 29.48213555981122 20.829160022756227 20.755709756599174 28.932994660833376 26 28.882781387969647 21.5169216058632 21.69346933651707 27.90682766965008 27 26.527832309035986 20.71511179130509 23.355582086682 29.401473812976924 28 31.268980216504676 23.80189261303996 19.86255452012938 25.066572650325984 29 30.67122859592471 22.949869525618116 21.041230973042417 25.337670905414754 30 29.223590622938385 24.422347043159377 20.410894143476416 25.943168190425826 31 32.946851387408756 23.19319022550567 19.216726361701163 24.643232025384414 32 31.16561566013093 23.3048346300644 22.39511969722465 23.134430012580026 33 35.28951424000341 21.83155583689233 19.298456476043192 23.580473447061053 34 29.092448511363422 25.167800471684252 21.44721062598323 24.29254039096909 35 29.236143941154317 25.997387841443576 22.370814336423585 22.395653880978518 36 32.344559204920905 24.773839953205503 20.406353581568524 22.47524726030507 37 30.263913483599225 25.970945745627038 20.925847282206604 22.839293488567133 38 33.56570326626656 24.032927086588515 19.960310147087494 22.441059500057424 39 29.849386890596495 24.79280347646787 19.609084328918307 25.748725304017327 40 28.745496163225187 25.101561686204438 22.324607441713876 23.8283347088565 41 27.31361661097801 25.58179288093311 20.386321690798418 26.718268817290458 42 27.398284735966328 22.886568750784583 22.66755341169809 27.047593101551005 43 27.1221117352158 21.8881793148025 25.246058391626136 25.74365055835557 44 26.24631747074676 22.280003098265773 24.644834576646023 26.828844854341444 45 28.205169296186195 22.89030803706167 21.914354318742106 26.99016834801003 46 28.486951226352353 23.05643918451508 23.836347465164543 24.620262123968022 47 29.812261119702566 23.027326169929193 22.398858983501736 24.761553726866506 48 30.090570855468574 22.50596282615257 22.33395565740659 25.069510660972266 49 28.2243999113255 23.675023971495953 21.43519149152117 26.665384625657378 50 24.5908820175052 25.719345197554507 23.00676009540522 26.683012689535072 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 2.0 3 1.5 4 1.0 5 2.0 6 3.0 7 258.0 8 513.0 9 624.5 10 736.0 11 857.5 12 979.0 13 879.0 14 779.0 15 758.0 16 737.0 17 994.5 18 1252.0 19 1544.5 20 1837.0 21 2234.5 22 2632.0 23 2805.0 24 2978.0 25 3366.0 26 3754.0 27 4200.0 28 4646.0 29 4686.0 30 4726.0 31 4325.5 32 3925.0 33 4344.0 34 4763.0 35 5060.5 36 5358.0 37 5717.0 38 6076.0 39 6896.5 40 7717.0 41 8414.5 42 9112.0 43 11655.0 44 14198.0 45 15145.5 46 16093.0 47 19238.5 48 22384.0 49 21956.5 50 21529.0 51 23716.5 52 25904.0 53 28675.5 54 31447.0 55 31249.5 56 31052.0 57 31852.5 58 32653.0 59 31509.0 60 30365.0 61 27521.5 62 24678.0 63 21229.0 64 17780.0 65 15459.5 66 13139.0 67 12118.0 68 11097.0 69 8943.5 70 6790.0 71 7355.0 72 7920.0 73 5729.5 74 3539.0 75 2266.5 76 994.0 77 632.5 78 271.0 79 157.5 80 44.0 81 22.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 374403.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.50227161641333 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14493809176984 12.73066722221777 2 8.08448652585579 2.6682478505781204 3 2.9068544145019017 1.4390910329244155 4 1.378975479485312 0.910249116593617 5 0.7930727522861536 0.6543750984901296 6 0.5583879582422918 0.5528801852549259 7 0.38520676539613175 0.44497506697328815 8 0.3415068382293437 0.4508510882658526 9 0.2897143319575949 0.43028501374187705 >10 5.320061503601198 23.612524472293224 >50 1.8224488144371613 20.590914068530434 >100 0.9257910496075099 28.477335918782703 >500 0.040462895524803755 4.196547570398741 >1k 0.008092579104960751 2.8410562949549014 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGA 2976 0.7948654257578064 No Hit ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 2922 0.7804424644033301 No Hit ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 2066 0.5518118177471869 No Hit ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 1602 0.42788118684946436 No Hit ACTTATGGGGAAGATCAAGCCAAAGAATGCAAAACAGCCAGAACGTGAAG 1071 0.2860554001971138 No Hit ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 877 0.23423957607177295 No Hit ACTTATGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT 785 0.20966712339377624 No Hit ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 756 0.20192145896266855 No Hit ACTTATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG 749 0.20005181582412537 No Hit ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 731 0.1952441620392999 No Hit ACTTATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT 729 0.1947099782854304 No Hit ACTTATGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTC 706 0.18856686511593124 No Hit ACTTATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT 699 0.186697221977388 No Hit ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 663 0.17708191440773713 No Hit ACTTATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT 646 0.17254135249984642 No Hit ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 613 0.16372732056099978 No Hit ACTTATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTC 597 0.15945385053004385 No Hit ACTTATGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 597 0.15945385053004385 No Hit ACTTATGGGGGTCCCAAGATGGCGTCGTGGCTGCCGGAGACTCTGTTTGA 577 0.1541120129913489 No Hit ACTTATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC 570 0.15224236985280568 No Hit ACTTATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA 568 0.1517081860989362 No Hit ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA 559 0.14930435920652346 No Hit ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC 559 0.14930435920652346 No Hit ACTTATGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG 545 0.14556507292943702 No Hit ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 545 0.14556507292943702 No Hit ACTTATGGGAACCCTTCTCCACATGGCTATCGAGCCCATCCTAGCCGACG 543 0.1450308891755675 No Hit ACTTATGGGGTCGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCG 540 0.14422961354476324 No Hit ACTTATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA 525 0.14022323539074205 No Hit ACTTATGGGATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGA 517 0.13808650037526407 No Hit ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 516 0.13781940849832933 No Hit ACTTATGGGGGCGCTTCCGGTCCCTTCTCCCTCTGGCCGACGTGATTCCC 500 0.1335459384673734 No Hit ACTTATGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC 499 0.13327884659043865 No Hit ACTTATGGGGAGCGTCCCTTGTGTCGCCTGCCTCTGAAGTGGCATGGTCA 490 0.13087501969802592 No Hit ACTTATGGGGGCGGTACCCGCGGCAGAGTTAATCAGTTGGTCACTGCACC 489 0.13060792782109118 No Hit ACTTATGGGGGATCCAGGATAACGGAGGCTGGGATGCCTTTGTGGAACTA 476 0.12713573342093945 No Hit ACTTATGGGCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTTACG 476 0.12713573342093945 No Hit ACTTATGGGAGCCCAGCCCTGGGATTGGGGACCCGAGTGCAATTGCTCCT 471 0.12580027403626573 No Hit ACTTATGGGGGAAGCTTTGGTGGTGGTGTCTTGACACTTGGGTCATGTTC 466 0.124464814651592 No Hit ACTTATGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCA 464 0.12393063089772251 No Hit ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG 457 0.12206098775917927 No Hit ACTTATGGGGAGAAGGAGAAGAAAGTGGGGCTGTATACAATCTATGTGAA 449 0.1199242527437013 No Hit ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG 445 0.11885588523596231 No Hit ACTTATGGGGGCGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAG 434 0.1159178745896801 No Hit ACTTATGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGA 431 0.11511659895887585 No Hit ACTTATGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTG 429 0.11458241520500637 No Hit ACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCT 424 0.11324695582033263 No Hit ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA 422 0.11271277206646314 No Hit ACTTATGGGGTGTGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGG 409 0.10924057766631143 No Hit ACTTATGGGGGCACCATGGACTCGGTGCGTTCAGGGCCCTTCGGGCAGAT 409 0.10924057766631143 No Hit ACTTATGGGGGTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCC 408 0.10897348578937668 No Hit ACTTATGGGGCCAAAGCGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTG 406 0.1084393020355072 No Hit ACTTATGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA 399 0.10656965889696396 No Hit ACTTATGGGAGTTCAGGTTTGCCAGTAGAAAGCCTGTATCGTGGAGGACA 391 0.10443292388148599 No Hit ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 389 0.10389874012761649 No Hit ACTTATGGGCATTAGAAAGTAAAACTCAGCCGGGCGTGGTGGCACATGCC 388 0.10363164825068175 No Hit ACTTATGGGAGATCCGGAAGAAGATGATGGAAATCATGACCCGAGAGGTG 378 0.10096072948133428 No Hit ACTTATGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT 377 0.10069363760439953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0016025512616084808 0.0 0.0 0.0 0.0 9 0.005608929415629682 0.0 0.0 0.0 0.0 10 0.04807653784825442 0.0 0.0 0.0 0.0 11 0.09802271883505206 0.0 0.0 0.0 0.0 12 0.13274466283656916 0.0 0.0 0.0 0.0 13 0.15838548302230485 0.0 0.0 0.0 0.0 14 0.19257324326995243 0.0 0.0 0.0 0.0 15 0.21207095028618894 0.0 0.0 0.0 0.0 16 0.2334383004409687 0.0 0.0 0.0 0.0 17 0.2534701912110747 0.0 0.0 0.0 0.0 18 0.2745704494889197 0.0 0.0 0.0 0.0 19 0.29807453465917744 0.0 0.0 0.0 0.0 20 0.32478372235265207 0.0 0.0 0.0 0.0 21 0.3498903587845183 0.0 0.0 0.0 0.0 22 0.37873628149347094 0.0 0.0 0.0 0.0 23 0.415594960510466 0.0 0.0 0.0 0.0 24 0.4580625689430907 0.0 0.0 0.0 0.0 25 0.4949212479600858 0.0 0.0 0.0 0.0 26 0.5403268670389928 0.0 0.0 0.0 0.0 27 0.5940123343028768 0.0 0.0 0.0 0.0 28 0.709930208892557 0.0 0.0 0.0 0.0 29 0.7443850610171393 0.0 0.0 0.0 0.0 30 0.7919274151115242 0.0 0.0 0.0 0.0 31 0.8271835428669108 0.0 0.0 0.0 0.0 32 0.8570978330836024 0.0 0.0 0.0 0.0 33 0.8816702857615991 0.0 0.0 0.0 0.0 34 0.9179947810247246 0.0 0.0 0.0 0.0 35 0.9850348421353461 0.0 0.0 0.0 0.0 36 1.0152162242289726 0.0 0.0 0.0 0.0 37 1.0405899525377735 0.0 0.0 0.0 0.0 38 1.0681004158620524 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTACA 75 0.0 44.000004 33 TCCGACC 65 0.0 44.000004 23 ATAATCT 75 0.0 44.000004 40 ACGGCCA 75 0.0 44.000004 11 CTTACAA 75 0.0 44.000004 34 GTGTTAT 20 7.8496744E-4 44.0 13 ACTATTT 20 7.8496744E-4 44.0 10 AACGTAC 25 4.4370732E-5 44.0 33 AGCGTGC 45 4.783942E-10 44.0 16 TAATAGA 25 4.4370732E-5 44.0 21 AACAACG 20 7.8496744E-4 44.0 44 AACCGAC 20 7.8496744E-4 44.0 10 GATACCG 55 1.8189894E-12 44.0 42 TCGTTAC 30 2.5231275E-6 44.0 39 GGTACGC 20 7.8496744E-4 44.0 9 ACAGTCG 25 4.4370732E-5 44.0 10 GTACCCC 25 4.4370732E-5 44.0 30 CAAACGT 25 4.4370732E-5 44.0 17 TACCCCA 20 7.8496744E-4 44.0 13 AAATCAC 30 2.5231275E-6 44.0 10 >>END_MODULE