FastQCFastQC Report
Fri 17 Jun 2016
SRR1527500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301481
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG24040.7973968508794915No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGAAG19000.6302221367184001No Hit
ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC17810.5907503292081425No Hit
ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT12980.43054122813709655No Hit
ACTTATGGGGAAGATCAAGCCAAAGAATGCAAAACAGCCAGAACGTGAAGAA7760.25739598847025186No Hit
ACTTATGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA7200.23882102023013058No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG6540.21692909337570196No Hit
ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC6150.2039929547799032No Hit
ACTTATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC6100.20233447547274952No Hit
ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5920.19636394996699627No Hit
ACTTATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA5840.19371038307555036No Hit
ACTTATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT5730.19006172859981227No Hit
ACTTATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC5720.18973003273838152No Hit
ACTTATGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG5620.18641307412407415No Hit
ACTTATGGGGGTCCCAAGATGGCGTCGTGGCTGCCGGAGACTCTGTTTGAAA5590.18541798653978195No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC5430.18011085275689015No Hit
ACTTATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC5360.177788981726875No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC4870.16153588451676887No Hit
ACTTATGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCC4860.16120418865533814No Hit
ACTTATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4820.1598774052096152No Hit
ACTTATGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG4670.15490196728815414No Hit
ACTTATGGGCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTTACGTG4490.14893144178240086No Hit
ACTTATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG4470.1482680500595394No Hit
ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC4310.14296091627664761No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA4270.14163413283092469No Hit
ACTTATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA4260.14130243696949393No Hit
ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA4200.13931226180090953No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4130.13699039077089437No Hit
ACTTATGGGGAGCGTCCCTTGTGTCGCCTGCCTCTGAAGTGGCATGGTCAGA4120.1366586949094636No Hit
ACTTATGGGGGATCCAGGATAACGGAGGCTGGGATGCCTTTGTGGAACTATA4120.1366586949094636No Hit
ACTTATGGGAACCCTTCTCCACATGGCTATCGAGCCCATCCTAGCCGACGGG4090.1356636073251714No Hit
ACTTATGGGGGAAGCTTTGGTGGTGGTGTCTTGACACTTGGGTCATGTTCAG4090.1356636073251714No Hit
ACTTATGGGGTCGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTC4030.13367343215658697No Hit
ACTTATGGGATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGAGA4010.13301004043372552No Hit
ACTTATGGGGGCGCTTCCGGTCCCTTCTCCCTCTGGCCGACGTGATTCCCGA4000.13267834457229477No Hit
ACTTATGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGC3990.13234664871086402No Hit
ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG3830.12703951492797225No Hit
ACTTATGGGAGCCCAGCCCTGGGATTGGGGACCCGAGTGCAATTGCTCCTGT3820.12670781906654152No Hit
ACTTATGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAAG3800.12604442734368004No Hit
ACTTATGGGGGCACCATGGACTCGGTGCGTTCAGGGCCCTTCGGGCAGATCT3770.12504933975938784No Hit
ACTTATGGGGGCGGTACCCGCGGCAGAGTTAATCAGTTGGTCACTGCACCCC3710.12305916459080339No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT3690.12239577286794193No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT3690.12239577286794193No Hit
ACTTATGGGGGCGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTC3680.1220640770065112No Hit
ACTTATGGGGAGAAGGAGAAGAAAGTGGGGCTGTATACAATCTATGTGAACC3630.1204055976993575No Hit
ACTTATGGGGGTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTG3580.11874711839220382No Hit
ACTTATGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT3500.11609355150075792No Hit
ACTTATGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAA3490.1157618556393272No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGCAAGAGTACCCGACTGCT3400.11277659288645055No Hit
ACTTATGGGGTGTGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGGAG3330.11045472185643539No Hit
ACTTATGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA3300.1094596342721432No Hit
ACTTATGGGGCCAAAGCGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTT3290.10912793841071244No Hit
ACTTATGGGCGAGAGGACACGGGCAAGATGGCGGTGGTGGCTGGTCTGGTGC3290.10912793841071244No Hit
ACTTATGGGAGTTCAGGTTTGCCAGTAGAAAGCCTGTATCGTGGAGGACACT3260.10813285082642023No Hit
ACTTATGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA3080.10216232532066698No Hit
ACTTATGGGGCTTTGCCGGCTGAGGGCACAGGGACACCCCCATGTAAGGATG3060.1014989335978055No Hit
ACTTATGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA3060.1014989335978055No Hit
ACTTATGGGCATTAGAAAGTAAAACTCAGCCGGGCGTGGTGGCACATGCCTT3040.10083554187494403No Hit
ACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT3030.10050384601351328No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA3030.10050384601351328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCA253.411118E-546.021
CTAGACC206.3040113E-446.033
GAAATAC206.3040113E-446.044
TTAGGCT206.3040113E-446.042
TTAGGAA206.3040113E-446.045
ACTATCA253.411118E-546.020
AACCGAC206.3040113E-446.010
CCTTACA206.3040113E-446.033
ATCTCGT206.3040113E-446.036
TCGTTAC206.3040113E-446.039
ATCTCAA206.3040113E-446.043
ACAGTCG206.3040113E-446.010
CGTATTG253.411118E-546.038
ATCTATA206.3040113E-446.024
TACCATG301.8568044E-646.023
TTGTGCA253.411118E-546.025
CTCCGAT301.8568044E-646.045
CCTAGTA253.411118E-546.040
CGCGGAT405.5879354E-946.025
ACTGAAT206.3040113E-446.021
Produced by FastQC (version 0.11.5)