FastQCFastQC Report
Fri 17 Jun 2016
SRR1527499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413912
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG35280.8523550899708151No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGAAG34340.8296449486847445No Hit
ACTTATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC23940.5783838110516245No Hit
ACTTATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT18290.4418813660874776No Hit
ACTTATGGGGAAGATCAAGCCAAAGAATGCAAAACAGCCAGAACGTGAAGAA13020.31455961653684844No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9690.23410773304470517No Hit
ACTTATGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA9030.218162314694911No Hit
ACTTATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC8870.21429675873132453No Hit
ACTTATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC8570.2070488412995999No Hit
ACTTATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC8410.20318328533601346No Hit
ACTTATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT7980.19279460368387483No Hit
ACTTATGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG7620.1840971027658053No Hit
ACTTATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC7570.18288911652718454No Hit
ACTTATGGGGGTCCCAAGATGGCGTCGTGGCTGCCGGAGACTCTGTTTGAAA7200.17395001836139082No Hit
ACTTATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7180.1734668238659425No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7170.17322522661821835No Hit
ACTTATGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCC7110.17177564313187343No Hit
ACTTATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA6990.1688764761591836No Hit
ACTTATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC6930.16742689267283867No Hit
ACTTATGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG6750.1630781422138039No Hit
ACTTATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC6680.16138696147973483No Hit
ACTTATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG6400.15462223854345852No Hit
ACTTATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT6190.14954869634125129No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA6130.14809911285490637No Hit
ACTTATGGGGAGCGTCCCTTGTGTCGCCTGCCTCTGAAGTGGCATGGTCAGA6110.14761591835945803No Hit
ACTTATGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGC6020.14544154312994068No Hit
ACTTATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA5950.14375036239587158No Hit
ACTTATGGGGTCGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTC5920.14302557065269914No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGGAG5880.1420591816618025No Hit
ACTTATGGGGGATCCAGGATAACGGAGGCTGGGATGCCTTTGTGGAACTATA5730.13843522294594018No Hit
ACTTATGGGCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTTACGTG5690.13746883395504358No Hit
ACTTATGGGAACCCTTCTCCACATGGCTATCGAGCCCATCCTAGCCGACGGG5620.13577765322097451No Hit
ACTTATGGGGGAAGCTTTGGTGGTGGTGTCTTGACACTTGGGTCATGTTCAG5620.13577765322097451No Hit
ACTTATGGGAGCCCAGCCCTGGGATTGGGGACCCGAGTGCAATTGCTCCTGT5570.13456966698235373No Hit
ACTTATGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT5530.13360327799145713No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5530.13360327799145713No Hit
ACTTATGGGGGCGGTACCCGCGGCAGAGTTAATCAGTTGGTCACTGCACCCC5450.13167050000966388No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT5390.13022091652331896No Hit
ACTTATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5310.12828813854152574No Hit
ACTTATGGGATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGAGA5300.12804654129380158No Hit
ACTTATGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAA5300.12804654129380158No Hit
ACTTATGGGGAGAAGGAGAAGAAAGTGGGGCTGTATACAATCTATGTGAACC5280.12756334679835327No Hit
ACTTATGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAAG5170.12490577707338758No Hit
ACTTATGGGGTGTGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGGAG5100.12321459633931849No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT5060.12224820734842189No Hit
ACTTATGGGGGCGCTTCCGGTCCCTTCTCCCTCTGGCCGACGTGATTCCCGA5020.12128181835752527No Hit
ACTTATGGGGGCACCATGGACTCGGTGCGTTCAGGGCCCTTCGGGCAGATCT4710.1137923036780765No Hit
ACTTATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG4680.11306751193490405No Hit
ACTTATGGGCGAGAGGACACGGGCAAGATGGCGGTGGTGGCTGGTCTGGTGC4680.11306751193490405No Hit
ACTTATGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT4600.1111347339531108No Hit
ACTTATGGGAGTTCTCACGGAGTCTGGCAGGAGTGCTCACCAAGTGGCCAGG4590.11089313670538666No Hit
ACTTATGGGGGTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTG4520.10920195597131759No Hit
ACTTATGGGAGTTCAGGTTTGCCAGTAGAAAGCCTGTATCGTGGAGGACACT4500.10871876147586926No Hit
ACTTATGGGGCCAAAGCGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTT4470.10799396973269682No Hit
ACTTATGGGGGCGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTC4460.10775237248497266No Hit
ACTTATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA4370.10557799725545527No Hit
ACTTATGGGCATTAGAAAGTAAAACTCAGCCGGGCGTGGTGGCACATGCCTT4370.10557799725545527No Hit
ACTTATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT4350.10509480276000695No Hit
ACTTATGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG4350.10509480276000695No Hit
ACTTATGGGAGCTGCTTCTGGGAAGATGGTGGTTGTTTAGCTTGGAGGACAG4350.10509480276000695No Hit
ACTTATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGC4300.1038868165213862No Hit
ACTTATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT4260.10292042753048958No Hit
ACTTATGGAGAACAAGACTCCCATGTGGCCATTGCCGAGTGTAACTGTTGCT4240.10243723303504125No Hit
ACTTATGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG4170.10074605230097218No Hit
ACTTATGGGGCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTC4150.10026285780552388No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA206.306752E-446.028
ATTTAAC206.306752E-446.037
CTTCGCA206.306752E-446.021
CATGGCA206.306752E-446.044
CGTGGAC301.858496E-646.042
TAATAGA253.413341E-546.021
TCTGATC253.413341E-546.014
TCGTTTA253.413341E-546.033
AACAAAT301.858496E-646.035
CTCACTA405.5970304E-946.022
ACTATCA206.306752E-446.020
ACAGTTC206.306752E-446.038
GATACGT253.413341E-546.037
CCTTACA301.858496E-646.033
TCGTTAC206.306752E-446.039
GTCGCAT206.306752E-446.09
TCGCAAC453.092282E-1046.016
CGAACAT206.306752E-446.037
CATATTG301.858496E-646.036
GCGACGA253.413341E-546.013
Produced by FastQC (version 0.11.5)