FastQCFastQC Report
Fri 17 Jun 2016
SRR1527498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235912
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC99360.8039407336444666No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC73460.5943788878172556No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG57060.4616833560965506No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT42920.3472739159422354No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT27890.22566331583478436No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25260.20438348361372008No Hit
CATGATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA22410.1813235893817683No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20990.1698340982205853No Hit
CATGATGGGCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATG19050.1541371877609409No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15770.1275980814167999No Hit
CATGATGGGCGGGCAACTTGAAGGCATCCTGAGAGAACTCAAAGAAGTGG13500.10923107794082426No Hit
CATGATGGGATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCT13120.10615642537656403No Hit
CATGATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTG12990.10510457055194868No Hit
CATGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC12740.10308177281230378No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTAC12530.10138262271100207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA207.856711E-444.00000431
CGAACCC207.856711E-444.00000440
CGAACCA207.856711E-444.00000416
ACGCCGT408.312782E-944.00000443
CTCGTAC207.856711E-444.00000440
CGTATAG207.856711E-444.00000419
TCTTCGA207.856711E-444.00000421
CGCGCGA207.856711E-444.00000443
CGCGCAC207.856711E-444.00000417
ATCCGAC207.856711E-444.00000432
TCGTCCG408.312782E-944.00000412
TTTGCGA207.856711E-444.00000438
CGGTATC207.856711E-444.00000411
CGTCCGA408.312782E-944.00000413
TCGACCC207.856711E-444.00000414
TTCGATC207.856711E-444.00000413
TTCGAGT207.856711E-444.00000418
TAAGACG207.856711E-444.00000414
TCGCGCC207.856711E-444.00000442
ACGACGT207.856711E-444.00000424
Produced by FastQC (version 0.11.5)