##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527498_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1235912
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.373771757212488	31.0	31.0	33.0	30.0	33.0
2	30.172644977959596	31.0	30.0	33.0	26.0	33.0
3	31.495705195839186	31.0	31.0	33.0	30.0	34.0
4	35.52559162788289	35.0	35.0	37.0	35.0	37.0
5	34.55105865142502	35.0	35.0	37.0	32.0	37.0
6	35.58403834577219	36.0	35.0	37.0	35.0	37.0
7	36.424287489724186	37.0	36.0	37.0	35.0	37.0
8	36.693375418314574	37.0	37.0	37.0	35.0	37.0
9	38.67803775673349	39.0	39.0	39.0	39.0	39.0
10	36.939905915631535	39.0	37.0	39.0	33.0	39.0
11	37.2933072904867	39.0	37.0	39.0	34.0	39.0
12	37.164755257655884	39.0	37.0	39.0	34.0	39.0
13	37.19218682236276	39.0	37.0	39.0	34.0	39.0
14	37.872861498229646	40.0	38.0	40.0	34.0	40.0
15	37.91964233699487	40.0	38.0	40.0	34.0	40.0
16	38.0536801972956	40.0	38.0	40.0	34.0	40.0
17	37.98762533254795	40.0	38.0	40.0	34.0	40.0
18	37.868417816155194	40.0	37.0	40.0	34.0	40.0
19	37.84715254807786	40.0	37.0	40.0	34.0	40.0
20	37.66689861414081	39.0	37.0	40.0	33.0	40.0
21	37.57980341642447	39.0	37.0	40.0	33.0	40.0
22	37.45803584721242	39.0	36.0	40.0	34.0	40.0
23	37.262640058515494	38.0	36.0	40.0	33.0	40.0
24	37.163000278336966	38.0	36.0	40.0	33.0	40.0
25	36.99223973875163	38.0	36.0	40.0	33.0	40.0
26	36.41453436814272	38.0	35.0	40.0	32.0	40.0
27	36.33191602638376	38.0	35.0	39.0	32.0	40.0
28	36.22994598320916	38.0	35.0	39.0	32.0	40.0
29	35.925521396345374	37.0	34.0	39.0	31.0	40.0
30	35.60687815961007	37.0	34.0	38.0	31.0	40.0
31	35.51977567982186	37.0	34.0	38.0	31.0	40.0
32	35.42630705098745	37.0	34.0	38.0	31.0	40.0
33	34.98874596249571	36.0	34.0	38.0	30.0	40.0
34	34.47078109121038	36.0	33.0	38.0	29.0	39.0
35	34.35771478875519	35.0	33.0	38.0	29.0	39.0
36	34.16306905346012	35.0	33.0	38.0	29.0	39.0
37	33.93873107470435	35.0	32.0	38.0	29.0	39.0
38	33.44222970567484	35.0	31.0	37.0	27.0	38.0
39	32.728674047990474	34.0	31.0	37.0	25.0	38.0
40	32.57882276408029	34.0	31.0	37.0	25.0	38.0
41	32.28479373936008	34.0	30.0	36.0	25.0	38.0
42	31.945003365935438	33.0	30.0	36.0	24.0	38.0
43	31.46180634219912	33.0	29.0	36.0	24.0	38.0
44	31.12064936662157	33.0	29.0	35.0	23.0	38.0
45	30.743045621371103	33.0	28.0	35.0	23.0	37.0
46	30.564075759439184	32.0	28.0	35.0	23.0	37.0
47	30.197897584941323	32.0	27.0	35.0	22.0	37.0
48	29.717097171966937	31.0	27.0	35.0	20.0	37.0
49	29.21562700256976	31.0	26.0	34.0	19.0	36.0
50	32.6432650544699	34.0	31.0	37.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	8.0
15	23.0
16	56.0
17	119.0
18	228.0
19	351.0
20	583.0
21	970.0
22	1450.0
23	2299.0
24	3373.0
25	5048.0
26	7210.0
27	10255.0
28	14797.0
29	20818.0
30	29727.0
31	41386.0
32	64449.0
33	97971.0
34	136799.0
35	291828.0
36	413905.0
37	90307.0
38	1944.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.38490766332878	0.4480092433765511	0.08689939089514465	0.08018370239952359
8	99.40618749554984	0.4980937154101587	0.07476260445727527	0.0209561845827211
9	97.24260303322568	1.7277120053854966	0.4167772462764339	0.6129077151124028
10	39.357656532180286	50.23788101418224	3.7416903468855387	6.6627721067519365
11	30.357986652771395	23.407087235984438	31.887221743942934	14.347704367301231
12	31.693033160937023	18.41352782398747	26.615244451061244	23.278194564014264
13	26.680054890639465	20.420628653172717	29.469088414061844	23.43022804212598
14	26.249765355462202	22.25352614101975	29.303380823230135	22.193327680287915
15	25.822469560939616	25.026134546796214	27.912828745088646	21.238567147175527
16	28.91751192641547	27.15646421428063	25.633216604418436	18.29280725488546
17	28.81022273430471	26.661526063344315	24.09936953440051	20.428881667950467
18	25.480535831030043	27.1401200085443	26.600194835878284	20.779149324547376
19	26.64752830298597	27.334794063007724	25.631760190045895	20.38591744396041
20	25.48223498113134	29.50015858734279	24.885186000297757	20.132420431228113
21	24.10584248716737	29.446675815106577	27.54710691376085	18.9003747839652
22	23.533957110214967	27.1583251882011	28.745331382816904	20.562386318767032
23	22.906080691829192	28.691363139123172	29.045595479289787	19.356960689757845
24	24.43709584501162	29.12092446711416	26.24515337661581	20.196826311258405
25	24.714866430619654	29.90730731637851	24.238780754616833	21.139045498385
26	21.67977979014687	31.72426515803714	25.297836739185314	21.298118312630674
27	20.575251312391174	28.260102661030885	27.34830635190855	23.816339674669393
28	21.34342898199872	31.014829534788884	25.040132307154554	22.601609176057842
29	23.65896601052502	30.79604373126889	24.45958935587647	21.085400902329614
30	24.509673827910078	28.017771491821424	26.03284052586268	21.439714154405813
31	23.47060308500929	28.632621092763888	26.667270808924908	21.229505013301917
32	26.04416819320469	28.891862851076777	24.284900543080738	20.779068412637795
33	26.054848565270017	28.60211730285004	25.108664694573722	20.234369437306217
34	22.61010492656435	30.36866702483672	28.026995449514203	18.99423259908472
35	21.16728375483044	33.580060716296956	25.842454802607307	19.410200726265302
36	21.906252225077512	33.54025205678074	24.80912880528711	19.744366912854634
37	23.304248198900893	32.13367942054127	25.365560007508627	19.196512373049213
38	23.472706794658517	32.84796975836468	23.819818886781583	19.859504560195223
39	23.323586145291898	30.625805073500377	24.29558091514606	21.75502786606166
40	23.050913009987767	30.588585595090912	24.005107159733054	22.355394235188268
41	21.218015522140735	31.88705992012376	24.158192492669382	22.73673206506612
42	19.908213529765874	31.823948630646843	24.623355060878122	23.644482778709165
43	20.64151816634194	30.563502903119318	25.23205535669206	23.562923573846682
44	21.96944442646402	30.950261830939418	23.974117898361698	23.106175844234862
45	22.931163383800786	30.86077325893753	23.271074315970715	22.936989041290964
46	20.83166115386856	33.63475716717695	24.335551398481446	21.19803028047304
47	20.780848474648682	31.813106434762346	25.386839839729692	22.019205250859283
48	21.045268595175063	31.542375185288275	25.849170491102925	21.56318572843374
49	22.919997540277947	31.787700095152406	24.244201852559083	21.048100512010564
50	21.54894523234664	31.595615221795725	24.19670656163222	22.658732984225413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	3.0
6	4.0
7	32.0
8	60.0
9	1677.5
10	3295.0
11	8609.5
12	13924.0
13	16139.0
14	18354.0
15	16388.0
16	14422.0
17	13137.0
18	11852.0
19	11920.5
20	11989.0
21	10887.5
22	9786.0
23	11095.0
24	12404.0
25	15880.5
26	19357.0
27	18954.5
28	18552.0
29	22557.5
30	26563.0
31	28318.5
32	30074.0
33	32712.5
34	35351.0
35	35224.0
36	35097.0
37	42426.0
38	49755.0
39	51580.5
40	53406.0
41	53306.5
42	53207.0
43	57437.0
44	61667.0
45	64123.5
46	66580.0
47	69247.0
48	71914.0
49	69787.5
50	67661.0
51	73352.0
52	79043.0
53	79252.0
54	79461.0
55	80921.5
56	82382.0
57	83237.0
58	84092.0
59	79260.0
60	74428.0
61	71430.0
62	68432.0
63	51780.5
64	35129.0
65	28165.5
66	21202.0
67	16862.5
68	12523.0
69	9426.0
70	6329.0
71	5010.5
72	3692.0
73	3083.0
74	2474.0
75	1603.0
76	732.0
77	599.5
78	467.0
79	309.5
80	152.0
81	123.0
82	94.0
83	48.5
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1235912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.52032776866457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.46998087967839	7.624079522430711
2	9.36605049034585	1.9706784211259976
3	3.1330417813393687	0.9888187935783261
4	1.4602677042372199	0.6144997951428356
5	0.7347727139237409	0.386502489297448
6	0.4686558136855033	0.2958247662397014
7	0.3634591815505551	0.26765968043096827
8	0.3132500386337449	0.2636394463979069
9	0.27928823018087773	0.26443833510897635
>10	6.81464646618599	18.958648530662256
>50	2.4834529352246713	18.20357602356705
>100	2.0421559240570692	42.17216023497857
>500	0.057090872074130376	3.91235537144922
>1k	0.011572474069080482	2.2113171814022783
>5k	0.002314494813816096	1.8658014081877616
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9936	0.8039407336444666	No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	7346	0.5943788878172556	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5706	0.4616833560965506	No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	4292	0.3472739159422354	No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2789	0.22566331583478436	No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2526	0.20438348361372008	No Hit
CATGATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	2241	0.1813235893817683	No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2099	0.1698340982205853	No Hit
CATGATGGGCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATG	1905	0.1541371877609409	No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1577	0.1275980814167999	No Hit
CATGATGGGCGGGCAACTTGAAGGCATCCTGAGAGAACTCAAAGAAGTGG	1350	0.10923107794082426	No Hit
CATGATGGGATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCT	1312	0.10615642537656403	No Hit
CATGATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTG	1299	0.10510457055194868	No Hit
CATGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	1274	0.10308177281230378	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTAC	1253	0.10138262271100207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0014564143725443236	0.0	0.0	0.0	0.0
8	0.0020227977396448937	0.0	0.0	0.0	0.0
9	0.0030746525642602386	0.0	0.0	0.0	0.0
10	0.01771970819928927	0.0	0.0	0.0	0.0
11	0.029451935089229653	0.0	0.0	0.0	0.0
12	0.03916136423952515	0.0	0.0	0.0	0.0
13	0.0457961408255604	0.0	0.0	0.0	0.0
14	0.0553437461566843	0.0	0.0	0.0	0.0
15	0.0648913514878082	0.0	0.0	0.0	0.0
16	0.0759762831010622	0.0	0.0	0.0	0.0
17	0.08269197159668326	0.0	0.0	0.0	0.0
18	0.09086407446484863	0.0	0.0	0.0	0.0
19	0.09992620833845775	0.0	0.0	0.0	0.0
20	0.10939290175999586	0.0	0.0	0.0	0.0
21	0.11853594754319077	0.0	0.0	0.0	0.0
22	0.12654622659218456	0.0	0.0	0.0	0.0
23	0.1352847128274505	0.0	0.0	0.0	0.0
24	0.14572234916401816	0.0	0.0	0.0	0.0
25	0.1541371877609409	0.0	0.0	0.0	0.0
26	0.16554576701253812	0.0	0.0	0.0	0.0
27	0.17768255345040748	0.0	0.0	0.0	0.0
28	0.19791053084685642	0.0	0.0	0.0	0.0
29	0.21037096492306895	0.0	0.0	0.0	0.0
30	0.2248541967389264	0.0	0.0	0.0	0.0
31	0.23877104518768327	0.0	0.0	0.0	0.0
32	0.251959686450168	0.0	0.0	0.0	0.0
33	0.2647437681647237	0.0	0.0	0.0	0.0
34	0.27663781887383565	0.0	0.0	0.0	0.0
35	0.29492391044022553	0.0	0.0	0.0	0.0
36	0.3107017328094557	0.0	0.0	0.0	0.0
37	0.32631773135951425	0.0	0.0	0.0	0.0
38	0.3415291703616439	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	20	7.856711E-4	44.000004	31
CGAACCC	20	7.856711E-4	44.000004	40
CGAACCA	20	7.856711E-4	44.000004	16
ACGCCGT	40	8.312782E-9	44.000004	43
CTCGTAC	20	7.856711E-4	44.000004	40
CGTATAG	20	7.856711E-4	44.000004	19
TCTTCGA	20	7.856711E-4	44.000004	21
CGCGCGA	20	7.856711E-4	44.000004	43
CGCGCAC	20	7.856711E-4	44.000004	17
ATCCGAC	20	7.856711E-4	44.000004	32
TCGTCCG	40	8.312782E-9	44.000004	12
TTTGCGA	20	7.856711E-4	44.000004	38
CGGTATC	20	7.856711E-4	44.000004	11
CGTCCGA	40	8.312782E-9	44.000004	13
TCGACCC	20	7.856711E-4	44.000004	14
TTCGATC	20	7.856711E-4	44.000004	13
TTCGAGT	20	7.856711E-4	44.000004	18
TAAGACG	20	7.856711E-4	44.000004	14
TCGCGCC	20	7.856711E-4	44.000004	42
ACGACGT	20	7.856711E-4	44.000004	24
>>END_MODULE