FastQCFastQC Report
Fri 17 Jun 2016
SRR1527497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012476
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC83380.823525693448536No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA68180.6733986780921227No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT44230.4368498611325108No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC34950.34519336754649No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23930.23635128141309028No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20490.20237516741137568No Hit
CATGATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG19860.19615279769594537No Hit
CATGATGGGCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGT15870.1567444561648869No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT14600.1442009489607655No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12980.12820056969251617No Hit
CATGATGGGCGGGCAACTTGAAGGCATCCTGAGAGAACTCAAAGAAGTGGCT11810.11664474022100278No Hit
CATGATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT11470.11328663593013562No Hit
CATGATGGGATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCTAT11010.10874331836013891No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACTC10200.10074312872601424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCCG405.6097633E-946.00000434
CGAAACT206.311095E-446.00000437
CGAAAAC206.311095E-446.00000428
GCGCGTT206.311095E-446.00000442
ACGCACC405.6097633E-946.00000439
TCTTCGA405.6097633E-946.00000421
ATTCGCT206.311095E-446.00000412
TAACGTT206.311095E-446.00000414
TAACGGG206.311095E-446.00000440
ACGGGAT206.311095E-446.00000414
AACGGGC206.311095E-446.00000441
CGTAGTA206.311095E-446.00000438
CTACGAG206.311095E-446.00000433
CGCTAGA206.311095E-446.00000431
CGAGCTT206.311095E-446.00000436
TCGTACG206.311095E-446.00000433
TATAGCG206.311095E-446.00000415
TAGGCGA206.311095E-446.00000432
CGCGTTT206.311095E-446.00000443
TAAATCG206.311095E-446.00000422