##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527497_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012476
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.982001548678685	33.0	31.0	33.0	31.0	34.0
2	31.366572639746522	31.0	31.0	33.0	30.0	34.0
3	32.27889747510064	33.0	31.0	34.0	31.0	34.0
4	36.153411043817336	37.0	35.0	37.0	35.0	37.0
5	35.36256365582987	35.0	35.0	37.0	33.0	37.0
6	36.13739189867216	37.0	35.0	37.0	35.0	37.0
7	36.64007245603847	37.0	37.0	37.0	35.0	37.0
8	36.769820716737975	37.0	37.0	37.0	37.0	37.0
9	38.761543977338725	39.0	39.0	39.0	39.0	39.0
10	37.50144200948961	39.0	37.0	39.0	35.0	39.0
11	37.83475855230149	39.0	37.0	39.0	35.0	39.0
12	37.711699832884925	39.0	37.0	39.0	35.0	39.0
13	37.67711728475539	39.0	37.0	39.0	35.0	39.0
14	38.34787194955732	40.0	38.0	40.0	35.0	40.0
15	38.42364065913661	40.0	38.0	40.0	35.0	40.0
16	38.53263879835176	40.0	38.0	40.0	35.0	40.0
17	38.513950947973086	40.0	38.0	40.0	35.0	40.0
18	38.41697383444151	40.0	38.0	40.0	35.0	40.0
19	38.40761262489185	40.0	38.0	40.0	35.0	40.0
20	38.324101509566646	40.0	38.0	40.0	34.0	40.0
21	38.29338571976027	40.0	38.0	40.0	34.0	40.0
22	38.332943200628954	40.0	38.0	40.0	35.0	40.0
23	38.264297622857235	40.0	38.0	40.0	35.0	40.0
24	38.245078401858414	40.0	38.0	40.0	35.0	40.0
25	38.27190471675378	40.0	38.0	40.0	35.0	40.0
26	38.18237370564833	40.0	38.0	40.0	35.0	40.0
27	38.172782367186976	40.0	38.0	40.0	35.0	40.0
28	38.121962397133366	40.0	38.0	40.0	35.0	40.0
29	38.080724876441515	40.0	38.0	40.0	35.0	40.0
30	37.95242652665347	40.0	37.0	40.0	34.0	40.0
31	37.90959291874573	40.0	37.0	40.0	34.0	40.0
32	37.86830601416725	40.0	37.0	40.0	34.0	40.0
33	37.70814814375847	40.0	36.0	40.0	34.0	40.0
34	37.37592594787432	39.0	36.0	40.0	33.0	40.0
35	37.39604593096528	39.0	36.0	40.0	33.0	40.0
36	37.39018307594451	39.0	35.0	40.0	34.0	40.0
37	37.3697430852682	39.0	35.0	40.0	34.0	40.0
38	37.44896175316748	40.0	35.0	40.0	34.0	40.0
39	37.44745949533618	40.0	35.0	40.0	34.0	40.0
40	37.39704842386387	40.0	35.0	40.0	34.0	40.0
41	37.30193703356919	40.0	35.0	40.0	34.0	40.0
42	37.22042892868572	40.0	35.0	40.0	33.0	40.0
43	37.28886808181132	40.0	35.0	40.0	33.0	40.0
44	37.17708469139021	39.0	35.0	40.0	33.0	40.0
45	37.11536075916861	39.0	35.0	40.0	33.0	40.0
46	36.98035805293163	39.0	35.0	40.0	33.0	40.0
47	36.92157937570866	39.0	35.0	40.0	33.0	40.0
48	36.80776630754704	39.0	35.0	40.0	33.0	40.0
49	36.730889423551766	39.0	35.0	40.0	33.0	40.0
50	36.996556955424126	39.0	35.0	40.0	33.0	40.0
51	37.06580205357954	39.0	35.0	40.0	33.0	40.0
52	36.75897897826714	39.0	35.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	5.0
15	9.0
16	13.0
17	33.0
18	91.0
19	117.0
20	227.0
21	358.0
22	532.0
23	872.0
24	1318.0
25	1834.0
26	2706.0
27	3690.0
28	5052.0
29	6496.0
30	9127.0
31	12717.0
32	18428.0
33	26554.0
34	60221.0
35	52569.0
36	91836.0
37	149429.0
38	451463.0
39	116775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.3729234075672	0.45413422145315047	0.08721194378928487	0.08573042719037291
8	99.42467772075585	0.48425839229769396	0.07200170670712194	0.019062180239334068
9	97.3218130602602	1.6748051311833565	0.4002070172527546	0.6031747913036951
10	39.543060773786245	50.09936037990036	3.738854056787519	6.618724789525874
11	30.661171227762434	23.200352403414996	31.80470450657596	14.33377186224661
12	31.806976165360958	18.312434072511348	26.527246077931725	23.35334368419597
13	26.859402099407788	20.24907257060908	29.446228848881358	23.445296481101774
14	26.46107166984699	22.027583863716277	29.333633587364044	22.17771087907269
15	25.95271394087366	24.80088416910623	27.982589216929586	21.263812673090523
16	29.052639272437077	27.096444755233705	25.579766829040885	18.271149143288334
17	28.87535111943394	26.504233186761954	24.16333819270778	20.45707750109632
18	25.58668057316914	26.99925726634508	26.648927974589025	20.765134185896752
19	26.88587186264168	27.205681912460143	25.47280133059944	20.43564489429873
20	25.516061615287672	29.454920412928303	24.821427865944475	20.207590105839547
21	24.31613193794223	29.259360221871926	27.537245327296645	18.887262512889194
22	23.739624445418954	27.050814043987216	28.708631118169713	20.500930392424117
23	23.06642330287335	28.34556078366302	29.168888941565037	19.419126971898592
24	24.677720755850014	28.789028085604006	26.149163041889388	20.384088116656592
25	24.997925876761524	29.615418044477103	24.125510135548893	21.26114594321248
26	21.648710685487853	31.58089673236699	25.518234506299407	21.25215807584575
27	20.687601483887025	28.107135379011456	27.300894045883556	23.904369091217966
28	21.348357887001765	30.808631513240808	25.091162654719717	22.75184794503771
29	23.757106341286114	30.554106961547728	24.46102426131582	21.22776243585033
30	24.685424642164357	27.677495565326982	26.143730814360044	21.493348978148617
31	23.520952595419544	28.44679775125534	26.629668258803175	21.402581394521945
32	26.286647782268417	28.63376514603803	24.308032980534847	20.771554091158702
33	26.21978199977086	28.258348839873737	25.174917726444875	20.346951433910533
34	22.796194675231806	30.174641176679746	28.05972684784627	18.969437300242177
35	21.313394095267444	33.264788498690336	25.93740493601824	19.484412470023983
36	22.08141230014341	33.25589939909687	24.914269572809626	19.748418727950096
37	23.515717903436723	31.813099767303125	25.355366448192353	19.3158158810678
38	23.601843401720142	32.66260138511925	23.92224605817817	19.813309154982438
39	23.306033920804047	30.399831699714362	24.448974592978008	21.845159786503583
40	23.312355058292738	30.153406105428672	24.094497054745002	22.439741781533588
41	21.324949924738956	31.581193035686773	24.2330682406299	22.86078879894437
42	20.22566460834627	31.432448769156007	24.570162650769007	23.77172397172871
43	20.713774943801138	30.332669613897025	25.230820286110482	23.72273515619136
44	22.142747087338364	30.630651985824848	24.088669756122613	23.13793117071417
45	23.052101975750535	30.495735207550602	23.28242842299472	23.169734393704147
46	21.045338358637636	33.41837238611088	24.27346425989357	21.262824995357914
47	21.042869164306115	31.574378059331774	25.30924189807956	22.073510878282544
48	21.316752199558312	31.15086184758947	25.834488916280485	21.69789703657173
49	23.118375151608532	31.386027915723435	24.4333692848028	21.062227647865235
50	21.7309842406141	31.180788482887493	24.269711084509655	22.81851619198875
51	20.704984611980926	31.45595549919208	25.757647588683586	22.08141230014341
52	20.872000916564936	30.667196061931346	26.936045891458164	21.52475713004555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	33.0
8	64.0
9	1388.0
10	2712.0
11	6930.0
12	11148.0
13	13078.0
14	13078.0
15	11148.0
16	10014.5
17	8881.0
18	8965.0
19	9049.0
20	8237.0
21	7425.0
22	7726.0
23	8027.0
24	10603.0
25	13179.0
26	13117.0
27	13055.0
28	16161.0
29	19267.0
30	20434.5
31	21602.0
32	22704.5
33	23807.0
34	24315.0
35	24823.0
36	30429.5
37	36036.0
38	36281.5
39	39259.0
40	41991.0
41	43323.0
42	44655.0
43	45679.0
44	46703.0
45	48415.0
46	50127.0
47	52661.0
48	55195.0
49	55390.0
50	55585.0
51	60202.0
52	64819.0
53	63944.0
54	63069.0
55	66923.0
56	70777.0
57	65395.0
58	60013.0
59	62118.0
60	64223.0
61	59325.0
62	54427.0
63	42829.5
64	25928.0
65	20624.0
66	17011.0
67	13398.0
68	9376.5
69	5355.0
70	4912.0
71	4469.0
72	3335.0
73	2201.0
74	1627.0
75	1053.0
76	706.0
77	359.0
78	339.0
79	319.0
80	179.0
81	39.0
82	59.0
83	79.0
84	41.5
85	4.0
86	2.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1012476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.304418080033502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.03667533570405	6.4234609017892765
2	8.87426357412027	1.6513971689205473
3	3.0072713762539145	0.8394273049435247
4	1.3916458786688604	0.5179382029796261
5	0.7908285122870337	0.3679099553964736
6	0.5891407037843002	0.32889668495845825
7	0.4999734621304602	0.3256373484408519
8	0.4139907648214002	0.30815545257369065
9	0.39382198397112683	0.32978559491780546
>10	9.750013268934769	23.253687000975827
>50	2.866089910302001	18.507599192474686
>100	2.3247173716894007	40.56362817489007
>500	0.046706650390106685	2.800066371943631
>1k	0.012738177379120004	2.285486273254872
>5k	0.0021230295631866677	1.4969243715406588
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	8338	0.823525693448536	No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6818	0.6733986780921227	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4423	0.4368498611325108	No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3495	0.34519336754649	No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2393	0.23635128141309028	No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2049	0.20237516741137568	No Hit
CATGATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1986	0.19615279769594537	No Hit
CATGATGGGCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGT	1587	0.1567444561648869	No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1460	0.1442009489607655	No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1298	0.12820056969251617	No Hit
CATGATGGGCGGGCAACTTGAAGGCATCCTGAGAGAACTCAAAGAAGTGGCT	1181	0.11664474022100278	No Hit
CATGATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT	1147	0.11328663593013562	No Hit
CATGATGGGATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCTAT	1101	0.10874331836013891	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACTC	1020	0.10074312872601424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.001777819918694369	0.0	0.0	0.0	0.0
8	0.0026667298780415536	0.0	0.0	0.0	0.0
9	0.003555639837388738	0.0	0.0	0.0	0.0
10	0.021926445663897218	0.0	0.0	0.0	0.0
11	0.035358862827365785	0.0	0.0	0.0	0.0
12	0.04543317569996721	0.0	0.0	0.0	0.0
13	0.05392720420039586	0.0	0.0	0.0	0.0
14	0.06518673035212687	0.0	0.0	0.0	0.0
15	0.07782900532950905	0.0	0.0	0.0	0.0
16	0.08849592484167526	0.0	0.0	0.0	0.0
17	0.09886654103405908	0.0	0.0	0.0	0.0
18	0.10815071172057411	0.0	0.0	0.0	0.0
19	0.11970654119208751	0.0	0.0	0.0	0.0
20	0.13047222847751452	0.0	0.0	0.0	0.0
21	0.13946009584424718	0.0	0.0	0.0	0.0
22	0.14785535657141502	0.0	0.0	0.0	0.0
23	0.15743583057771246	0.0	0.0	0.0	0.0
24	0.16760891122357469	0.0	0.0	0.0	0.0
25	0.1770906174566113	0.0	0.0	0.0	0.0
26	0.18667109146290875	0.0	0.0	0.0	0.0
27	0.19793061761463976	0.0	0.0	0.0	0.0
28	0.21906692109245057	0.0	0.0	0.0	0.0
29	0.23269687380244075	0.0	0.0	0.0	0.0
30	0.24583298764612693	0.0	0.0	0.0	0.0
31	0.25570976497220677	0.0	0.0	0.0	0.0
32	0.26548777452502575	0.0	0.0	0.0	0.0
33	0.275660855170888	0.0	0.0	0.0	0.0
34	0.28484625808414227	0.0	0.0	0.0	0.0
35	0.2984762107941324	0.0	0.0	0.0	0.0
36	0.3125012345971658	0.0	0.0	0.0	0.0
37	0.32524227734780875	0.0	0.0	0.0	0.0
38	0.33531659022041016	0.0	0.0	0.0	0.0
39	0.34292170876149164	0.0	0.0	0.0	0.0
40	0.35309478940735384	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCCG	40	5.6097633E-9	46.000004	34
CGAAACT	20	6.311095E-4	46.000004	37
CGAAAAC	20	6.311095E-4	46.000004	28
GCGCGTT	20	6.311095E-4	46.000004	42
ACGCACC	40	5.6097633E-9	46.000004	39
TCTTCGA	40	5.6097633E-9	46.000004	21
ATTCGCT	20	6.311095E-4	46.000004	12
TAACGTT	20	6.311095E-4	46.000004	14
TAACGGG	20	6.311095E-4	46.000004	40
ACGGGAT	20	6.311095E-4	46.000004	14
AACGGGC	20	6.311095E-4	46.000004	41
CGTAGTA	20	6.311095E-4	46.000004	38
CTACGAG	20	6.311095E-4	46.000004	33
CGCTAGA	20	6.311095E-4	46.000004	31
CGAGCTT	20	6.311095E-4	46.000004	36
TCGTACG	20	6.311095E-4	46.000004	33
TATAGCG	20	6.311095E-4	46.000004	15
TAGGCGA	20	6.311095E-4	46.000004	32
CGCGTTT	20	6.311095E-4	46.000004	43
TAAATCG	20	6.311095E-4	46.000004	22
>>END_MODULE