FastQCFastQC Report
Fri 17 Jun 2016
SRR1527496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324936
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC106980.807435227060024No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA86870.6556543108497316No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT54930.41458606302493106No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC47690.35994191417547716No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29860.2253693763321398No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26340.19880205534456002No Hit
CATGATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG22910.17291401245041269No Hit
CATGATGGGCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGT22290.16823454114010036No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT19570.14770524764969778No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16610.12536454591014207No Hit
CATGATGGGATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCTAT16200.12227005681783876No Hit
CATGATGGGCGGGCAACTTGAAGGCATCCTGAGAGAACTCAAAGAAGTGGCT16100.1215153033806916No Hit
CATGATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT15080.11381681832179064No Hit
CATGATGGGATGATAGGCTGCGGTGGCTTTCTGTGATGCAGCTGTTTTTGAG13610.1027219427957275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT351.01972546E-746.00000422
ACGCCAC700.046.00000419
AGCGCGA351.01972546E-746.00000446
TCGTCCG351.01972546E-746.00000412
ATGCGAG351.01972546E-746.00000413
TGGTCGA351.01972546E-746.00000424
TCGTTGC253.4174394E-546.037
TCGCATA206.311805E-446.037
GTCGCGA206.311805E-446.011
CGTATTG206.311805E-446.019
ACGCCGT206.311805E-446.043
ATAGGCG206.311805E-446.05
CGAAAGT453.110472E-1046.039
CATGCGA253.4174394E-546.024
GCGCGTT206.311805E-446.042
GCGAACT206.311805E-446.043
CGCATAT453.110472E-1046.026
GGCGTAG301.8616192E-646.08
TAGCGGA301.8616192E-646.015
CGGCACC206.311805E-446.042
Produced by FastQC (version 0.11.5)