##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1324936
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.877289921928305	31.0	31.0	33.0	30.0	33.0
2	29.706205431809536	31.0	30.0	31.0	25.0	33.0
3	30.984166782395526	31.0	31.0	33.0	28.0	34.0
4	35.336944576945605	35.0	35.0	37.0	33.0	37.0
5	34.248466341015714	35.0	35.0	37.0	32.0	37.0
6	35.39159702808286	35.0	35.0	37.0	33.0	37.0
7	36.309036059100215	37.0	35.0	37.0	35.0	37.0
8	36.58481013422535	37.0	37.0	37.0	35.0	37.0
9	38.63334983727516	39.0	39.0	39.0	38.0	39.0
10	36.70564163099199	39.0	37.0	39.0	32.0	39.0
11	37.101072051782126	39.0	37.0	39.0	34.0	39.0
12	37.02777568124045	39.0	37.0	39.0	34.0	39.0
13	36.98235160037919	39.0	37.0	39.0	33.0	39.0
14	37.627308036010795	40.0	37.0	40.0	33.0	40.0
15	37.71195438874029	40.0	37.0	40.0	33.0	40.0
16	37.804623015753215	40.0	37.0	40.0	33.0	40.0
17	37.83333685551604	40.0	37.0	40.0	33.0	40.0
18	37.76326932017849	40.0	37.0	40.0	33.0	40.0
19	37.72585996606629	40.0	37.0	40.0	33.0	40.0
20	37.586506819952056	40.0	37.0	40.0	33.0	40.0
21	37.62997080613705	40.0	37.0	40.0	33.0	40.0
22	37.672334361810684	40.0	37.0	40.0	33.0	40.0
23	37.684620993014	39.0	37.0	40.0	34.0	40.0
24	37.70892254418327	39.0	37.0	40.0	34.0	40.0
25	37.75352092478429	39.0	37.0	40.0	34.0	40.0
26	37.643135970341206	39.0	37.0	40.0	34.0	40.0
27	37.63014288992072	39.0	37.0	40.0	34.0	40.0
28	37.498053490885596	39.0	36.0	40.0	33.0	40.0
29	37.46706557901665	39.0	36.0	40.0	33.0	40.0
30	37.3871115284059	39.0	36.0	40.0	33.0	40.0
31	37.295365210093166	39.0	36.0	40.0	33.0	40.0
32	37.284528460242605	39.0	36.0	40.0	33.0	40.0
33	37.18742943055363	39.0	35.0	40.0	33.0	40.0
34	36.83603585380728	39.0	35.0	40.0	32.0	40.0
35	36.84055531738892	39.0	35.0	40.0	32.0	40.0
36	36.87622647433536	39.0	35.0	40.0	32.0	40.0
37	36.89332239444018	39.0	35.0	40.0	33.0	40.0
38	36.79611769927	38.0	35.0	40.0	32.0	40.0
39	36.74125693618409	38.0	35.0	40.0	32.0	40.0
40	37.00769320178484	39.0	35.0	40.0	33.0	40.0
41	37.0447319719594	39.0	35.0	40.0	33.0	40.0
42	37.088509935574244	39.0	35.0	40.0	33.0	40.0
43	37.1192865164808	39.0	35.0	40.0	33.0	40.0
44	37.03096602401927	39.0	35.0	40.0	33.0	40.0
45	36.99589640556223	39.0	35.0	40.0	33.0	40.0
46	36.78343255825187	39.0	35.0	40.0	33.0	40.0
47	36.7715776460146	39.0	35.0	40.0	33.0	40.0
48	36.73636160539075	39.0	35.0	40.0	33.0	40.0
49	36.690098993460815	39.0	35.0	40.0	33.0	40.0
50	36.58623058019406	39.0	35.0	40.0	32.0	40.0
51	36.47778760634476	39.0	35.0	40.0	32.0	40.0
52	36.05069754312661	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	8.0
15	12.0
16	27.0
17	74.0
18	115.0
19	216.0
20	341.0
21	613.0
22	1073.0
23	1618.0
24	2453.0
25	3745.0
26	5130.0
27	7279.0
28	9989.0
29	13691.0
30	18632.0
31	25619.0
32	36295.0
33	53822.0
34	93387.0
35	85528.0
36	149799.0
37	236778.0
38	517103.0
39	61587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.43718036191936	0.4380588949202075	0.05298369128773012	0.07177705187269423
8	99.47559731187016	0.4560975020680244	0.046115435009691036	0.02218975105212629
9	97.28326500298883	1.7163847914163401	0.38364117210189774	0.6167090334929386
10	39.52017304986807	50.11389229366551	3.7011599050822075	6.664774751384218
11	30.269914924192566	23.49841803679574	31.802743679694718	14.428923359316977
12	31.60401709969387	18.42677684054173	26.558188470990295	23.4110175887741
13	26.667023916626913	20.431401969604572	29.388136483573547	23.513437630194968
14	26.192057578630212	22.163334681826143	29.347153371936457	22.297454367607187
15	25.927667449597564	24.820972484708694	27.868591388565182	21.382768677128556
16	29.009552159500533	27.041155195420757	25.500854380890853	18.448438264187857
17	28.774823840547768	26.491543742490204	24.187281498879944	20.546350918082084
18	25.633162658422748	26.91556422347947	26.57788753569984	20.873385582397944
19	26.838126520828176	27.088931087992172	25.58387725897704	20.48906513220261
20	25.518213709945236	29.304056950675356	24.998792394500565	20.17893694487885
21	24.196489490813143	29.178541454077784	27.60963548427999	19.015333570829082
22	23.63880217610511	26.89435565189564	28.77006889389374	20.69677327810551
23	23.011828495866972	28.207173780469397	29.241487890735858	19.539509832927777
24	24.512806656321512	28.63209996558324	26.411087026090318	20.444006352004926
25	24.807915250246047	29.44693177632731	24.36215787026694	21.3829951031597
26	21.70089725088608	31.386044307045776	25.65271077244486	21.260347669623286
27	20.727416267653684	27.77922858160696	27.432947704643844	24.06040744609551
28	21.458772348249276	30.67416086512858	25.04354927332339	22.823517513298754
29	23.803111999372046	30.36682526552226	24.53975135402767	21.290311381078027
30	24.759988406987205	27.55401015596225	26.16028245892632	21.525718978124225
31	23.706729985448355	28.22430668349264	26.701516148704542	21.367447182354468
32	26.208888580278593	28.380540644982098	24.39348013790855	21.01709063683076
33	26.093033927676508	28.112452224107425	25.33405387128133	20.460459976934736
34	22.731286643279375	30.085604134841233	28.103093281486803	19.08001594039259
35	21.371900227633635	33.220925388094216	25.87906132824529	19.528113056026857
36	21.959551253796413	33.068238767759354	25.036681017045353	19.93552896139889
37	23.485662703707952	31.63511294130433	25.545611259713674	19.333613095274035
38	23.604385419371198	32.45696395901387	24.014065585054674	19.92458503656026
39	23.291238218298847	30.247498747109297	24.53522283340478	21.926040201187078
40	23.13168334168594	30.26199001310252	24.177545179540747	22.428781465670795
41	21.41009075155328	31.494426900620105	24.17294118357415	22.92254116425246
42	20.065572978619343	31.389591648200366	24.66639898078096	23.87843639239933
43	20.682055586081137	30.16304183749253	25.427114970081572	23.72778760634476
44	22.078123018772228	30.487963192184374	24.19724424425029	23.236669544793106
45	23.02639523720391	30.40682719769106	23.401205794091187	23.165571771013845
46	21.13264338805799	33.04204882349035	24.540204206089953	21.285103582361714
47	20.99150449531147	31.267170640695095	25.615350477306077	22.125974386687357
48	21.288801874203735	31.03561228617835	26.066617557376354	21.60896828224156
49	23.08639813545711	31.21720596315596	24.51008954394778	21.186306357439154
50	21.76633437388674	30.99281776629211	24.395668922876272	22.84517893694488
51	20.69835826032352	31.424612207683992	25.841927459137647	22.03510207285484
52	20.844101149036636	30.591213462386108	26.93986728415561	21.624818104421646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	3.5
6	6.0
7	42.5
8	79.0
9	1804.0
10	3529.0
11	8788.0
12	14047.0
13	16465.5
14	16542.5
15	14201.0
16	12733.0
17	11265.0
18	11282.5
19	11300.0
20	10444.0
21	9588.0
22	10024.0
23	10460.0
24	14132.5
25	17805.0
26	17286.0
27	16767.0
28	20888.0
29	25009.0
30	26716.0
31	28423.0
32	30075.5
33	31728.0
34	32157.0
35	32586.0
36	39792.0
37	46998.0
38	47402.0
39	51770.5
40	55735.0
41	57695.5
42	59656.0
43	60720.0
44	61784.0
45	63927.5
46	66071.0
47	69206.5
48	72342.0
49	73109.0
50	73876.0
51	79552.5
52	85229.0
53	83564.0
54	81899.0
55	87246.5
56	92594.0
57	85276.0
58	77958.0
59	81349.5
60	84741.0
61	77239.0
62	69737.0
63	55381.0
64	33749.5
65	26474.0
66	21995.0
67	17516.0
68	12268.5
69	7021.0
70	6254.5
71	5488.0
72	4161.5
73	2835.0
74	2092.0
75	1349.0
76	919.0
77	489.0
78	477.0
79	465.0
80	265.0
81	65.0
82	83.0
83	101.0
84	52.5
85	4.0
86	2.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1324936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.739505980327118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.22758965082912	6.137544397373799
2	9.467865389475993	1.6548893238451514
3	3.496299616617768	0.9166779422533917
4	1.5205832585808001	0.531565859278088
5	0.8815053041242283	0.38519604385418843
6	0.5336475018521728	0.27982893202942144
7	0.36584273227507524	0.22380993226040632
8	0.31913730525005496	0.2231281910222669
9	0.3104882151616607	0.24421622519037842
>10	7.2891196099110145	17.099136943719664
>50	2.9405560770987793	18.03072594804227
>100	2.535797181780477	44.7311632202893
>500	0.09600050722293074	5.461265832972392
>1k	0.012973041516612261	2.200439717790158
>5k	0.001729738868881635	1.0717997800997678
>10k+	8.648694344408175E-4	0.8086117099793594
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	10698	0.807435227060024	No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8687	0.6556543108497316	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5493	0.41458606302493106	No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4769	0.35994191417547716	No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2986	0.2253693763321398	No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2634	0.19880205534456002	No Hit
CATGATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2291	0.17291401245041269	No Hit
CATGATGGGCACATTATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGT	2229	0.16823454114010036	No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1957	0.14770524764969778	No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1661	0.12536454591014207	No Hit
CATGATGGGATTTTACAACTTTTTTCCTGGCTTTATTATATAAATTTTCTAT	1620	0.12227005681783876	No Hit
CATGATGGGCGGGCAACTTGAAGGCATCCTGAGAGAACTCAAAGAAGTGGCT	1610	0.1215153033806916	No Hit
CATGATGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT	1508	0.11381681832179064	No Hit
CATGATGGGATGATAGGCTGCGGTGGCTTTCTGTGATGCAGCTGTTTTTGAG	1361	0.1027219427957275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0012830808431501597	0.0	0.0	0.0	0.0
8	0.001962358936582597	0.0	0.0	0.0	0.0
9	0.0025661616863003195	0.0	0.0	0.0	0.0
10	0.017208378366955084	0.0	0.0	0.0	0.0
11	0.028756105955306522	0.0	0.0	0.0	0.0
12	0.03683196773278106	0.0	0.0	0.0	0.0
13	0.043700224010820146	0.0	0.0	0.0	0.0
14	0.05328559266258898	0.0	0.0	0.0	0.0
15	0.0627954859706431	0.0	0.0	0.0	0.0
16	0.07472059027756812	0.0	0.0	0.0	0.0
17	0.08415500824190754	0.0	0.0	0.0	0.0
18	0.09223087001938207	0.0	0.0	0.0	0.0
19	0.10219361538972449	0.0	0.0	0.0	0.0
20	0.1111751812917756	0.0	0.0	0.0	0.0
21	0.11894914169439127	0.0	0.0	0.0	0.0
22	0.12521359522271264	0.0	0.0	0.0	0.0
23	0.13276112959418418	0.0	0.0	0.0	0.0
24	0.1421955475585236	0.0	0.0	0.0	0.0
25	0.15034688467971283	0.0	0.0	0.0	0.0
26	0.16264936570521143	0.0	0.0	0.0	0.0
27	0.17276306176298326	0.0	0.0	0.0	0.0
28	0.1873298030999233	0.0	0.0	0.0	0.0
29	0.2005379882499985	0.0	0.0	0.0	0.0
30	0.21253856790063821	0.0	0.0	0.0	0.0
31	0.2222748872398365	0.0	0.0	0.0	0.0
32	0.23261500932875248	0.0	0.0	0.0	0.0
33	0.24250227935538018	0.0	0.0	0.0	0.0
34	0.25352167953772864	0.0	0.0	0.0	0.0
35	0.27050363187353954	0.0	0.0	0.0	0.0
36	0.283108014273897	0.0	0.0	0.0	0.0
37	0.29510859392453676	0.0	0.0	0.0	0.0
38	0.3046939625763056	0.0	0.0	0.0	0.0
39	0.3137510038220714	0.0	0.0	0.0	0.0
40	0.3243175519421315	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	35	1.01972546E-7	46.000004	22
ACGCCAC	70	0.0	46.000004	19
AGCGCGA	35	1.01972546E-7	46.000004	46
TCGTCCG	35	1.01972546E-7	46.000004	12
ATGCGAG	35	1.01972546E-7	46.000004	13
TGGTCGA	35	1.01972546E-7	46.000004	24
TCGTTGC	25	3.4174394E-5	46.0	37
TCGCATA	20	6.311805E-4	46.0	37
GTCGCGA	20	6.311805E-4	46.0	11
CGTATTG	20	6.311805E-4	46.0	19
ACGCCGT	20	6.311805E-4	46.0	43
ATAGGCG	20	6.311805E-4	46.0	5
CGAAAGT	45	3.110472E-10	46.0	39
CATGCGA	25	3.4174394E-5	46.0	24
GCGCGTT	20	6.311805E-4	46.0	42
GCGAACT	20	6.311805E-4	46.0	43
CGCATAT	45	3.110472E-10	46.0	26
GGCGTAG	30	1.8616192E-6	46.0	8
TAGCGGA	30	1.8616192E-6	46.0	15
CGGCACC	20	6.311805E-4	46.0	42
>>END_MODULE