FastQCFastQC Report
Fri 17 Jun 2016
SRR1527492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964841
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC748983.8119114981822957No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG474882.416887676916351No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT313621.5961596892572987No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT282221.4363503204585002No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT121630.6190322779298681No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC106940.5442679585778187No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT101100.5145454517693798No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC100440.5111864013423987No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT87530.44548133920251054No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG85200.4336228733011985No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC84670.43092545401892574No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC70250.3575352916597323No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA62490.3180410017909846No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG54800.2789029748463107No Hit
TAGCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA54500.2773761337431375No Hit
TAGCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG52470.2670445089449986No Hit
TAGCATGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC50880.25895225109818043No Hit
TAGCATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC48490.24678841697623372No Hit
TAGCATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA47920.24388741888020457No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTC47600.2422587883701531No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT47470.24159715722544473No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC37100.18881934975908993No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT36150.18398435293237467No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC31410.1598602635022376No Hit
TAGCATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC29460.14993579633161155No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGC29410.14968132281441604No Hit
TAGCATGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC29100.14810358700780368No Hit
TAGCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC28830.1467294300149478No Hit
TAGCATGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGG28710.1461186935736785No Hit
TAGCATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGC28660.14586422005648295No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCC28170.14337037958796667No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGC27740.141181907340085No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGCGTGTAAGAGCACCCGACTGT26520.13497275352051386No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT26320.13395485945173172No Hit
TAGCATGGGGGGGCAGGAGAGATGGCTCAGTGGATAAGAGCACAGACTGC26280.13375128063797528No Hit
TAGCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT26140.13303875478982777No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCTGACTG25660.1305958090247506No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT25610.13034133550755506No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTGCT25050.12749123211496502No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT24100.12265623528824977No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG22720.11563276621365291No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTG22610.11507292447582272No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCG22390.11395324100016235No Hit
TAGCATGGGGGGCATGGTGGCACACGCCTTTAATACCAGCACTCAGTAGG21480.10932182298720354No Hit
TAGCATGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT21350.10866019184249515No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT21320.10850750773217781No Hit
TAGCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA21110.10743871895995656No Hit
TAGCATGGGGAGCCGGGCGTGGTGGCGCATGCCTTTAATCCCAGCACTCG20410.103876089719219No Hit
TAGCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG20350.10357072149858437No Hit
TAGCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC19930.10143314395414182No Hit
TAGCATGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT19690.10021167107160324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG302.528639E-644.025
TCGCATA408.3182385E-944.023
ACACGTC254.4439967E-544.032
CGAACGA254.4439967E-544.010
GACGTTA502.7284841E-1144.09
CGCATTA207.857848E-444.015
CGACAAC207.857848E-444.040
CTCGGTT502.7284841E-1144.018
CGGATAC207.857848E-444.032
TTGGTCG207.857848E-444.022
GTATCGC207.857848E-444.039
CGTACCG254.4439967E-544.037
CGTAAAG254.4439967E-544.012
TTACGCC254.4439967E-544.030
CGATGCG207.857848E-444.022
ACCGTAC207.857848E-444.026
ACGTAGT207.857848E-444.021
CGGTATG207.857848E-444.014
CCACGTT302.528639E-644.020
TATCGCC207.857848E-444.040
Produced by FastQC (version 0.11.5)