##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527492_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1964841
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.613386528477367	31.0	31.0	33.0	30.0	34.0
2	30.903831913116633	31.0	31.0	33.0	28.0	34.0
3	31.90483046719811	31.0	31.0	34.0	30.0	34.0
4	36.04684297609832	37.0	35.0	37.0	35.0	37.0
5	35.30444448176723	37.0	35.0	37.0	33.0	37.0
6	35.872583074152054	37.0	35.0	37.0	35.0	37.0
7	36.51060569277616	37.0	37.0	37.0	35.0	37.0
8	36.69689506682729	37.0	37.0	37.0	35.0	37.0
9	38.66242255734688	39.0	39.0	39.0	39.0	39.0
10	37.8236529062657	39.0	38.0	39.0	35.0	39.0
11	37.56296514578025	39.0	37.0	39.0	35.0	39.0
12	36.36583214621437	37.0	35.0	39.0	33.0	39.0
13	36.21716057431619	37.0	35.0	39.0	33.0	39.0
14	36.744697408085436	39.0	35.0	40.0	33.0	40.0
15	36.76107837733435	38.0	35.0	40.0	33.0	40.0
16	36.868381716383155	38.0	35.0	40.0	33.0	40.0
17	36.756589973438054	38.0	35.0	40.0	33.0	40.0
18	36.651463400855334	38.0	35.0	40.0	33.0	40.0
19	36.57203509088013	38.0	35.0	40.0	32.0	40.0
20	36.36447529342069	38.0	34.0	40.0	32.0	40.0
21	36.35698511991556	38.0	34.0	40.0	32.0	40.0
22	36.337772878314325	38.0	35.0	40.0	32.0	40.0
23	36.270906907988994	37.0	35.0	40.0	32.0	40.0
24	36.23805692165422	37.0	35.0	40.0	32.0	40.0
25	36.05741329705559	37.0	34.0	39.0	32.0	40.0
26	35.596356651759606	36.0	34.0	39.0	31.0	40.0
27	35.57059120814356	36.0	34.0	39.0	31.0	40.0
28	35.52805494185026	36.0	34.0	38.0	31.0	40.0
29	35.25055767871294	35.0	34.0	38.0	31.0	40.0
30	34.949232024372456	35.0	34.0	38.0	31.0	40.0
31	34.7555517214879	35.0	34.0	38.0	30.0	40.0
32	34.59085544326487	35.0	34.0	38.0	30.0	39.0
33	33.71462067414106	35.0	32.0	38.0	27.0	39.0
34	32.92983859762698	34.0	31.0	38.0	23.0	39.0
35	32.86384954304191	34.0	31.0	37.0	23.0	39.0
36	32.96387290371079	34.0	31.0	37.0	25.0	38.0
37	33.07750550807928	34.0	31.0	37.0	27.0	38.0
38	32.69158624031156	34.0	31.0	36.0	27.0	38.0
39	32.21667503884538	34.0	31.0	36.0	25.0	38.0
40	32.183270809190155	34.0	31.0	36.0	25.0	38.0
41	31.935470096562522	34.0	30.0	35.0	24.0	38.0
42	31.500807444470063	33.0	30.0	35.0	24.0	38.0
43	30.68566056999014	32.0	29.0	35.0	23.0	38.0
44	30.10015110637451	32.0	27.0	34.0	22.0	37.0
45	29.683631398164025	31.0	26.0	34.0	20.0	37.0
46	29.79052757958532	31.0	26.0	34.0	20.0	37.0
47	29.58556392094831	31.0	26.0	34.0	20.0	37.0
48	29.44201948147458	31.0	26.0	34.0	20.0	36.0
49	29.158602655380257	31.0	26.0	34.0	20.0	36.0
50	32.20760204006329	34.0	31.0	36.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	7.0
14	22.0
15	57.0
16	170.0
17	402.0
18	711.0
19	1177.0
20	1688.0
21	2556.0
22	3876.0
23	5687.0
24	8238.0
25	11164.0
26	15686.0
27	21345.0
28	29292.0
29	40970.0
30	58785.0
31	88682.0
32	166484.0
33	313127.0
34	257357.0
35	312212.0
36	471913.0
37	149908.0
38	3317.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.37786314516035	0.5020253547233593	0.09949914522345575	0.020612354892838657
8	99.36844762502412	0.42573419426813675	0.14041848678849841	0.06539969391925352
9	97.78368834933717	1.4024035532646153	0.34715277215815427	0.4667553252400576
10	69.57178723367437	20.415545074639628	3.8315059590063525	6.181161732679642
11	53.46865217083724	14.811885541883541	16.046132994985346	15.67332929229388
12	48.06159887746642	11.97633803447709	19.37149112828977	20.590571959766717
13	33.780545092452776	11.939388479780298	22.704483467110062	31.575582960656867
14	27.906990947359102	13.094545563737729	34.15787842375032	24.840585065152855
15	36.420809622763365	13.787527845764618	30.281279757496915	19.510382773975095
16	44.501412582493955	16.69621104201307	24.09121145171543	14.711164923777547
17	39.33595644634858	19.870513695510223	24.179361078071963	16.614168780069228
18	39.40873587226651	22.907298860314906	21.73152942146464	15.952435845953946
19	35.100041173815086	31.309352766966896	19.17249283784286	14.418113221375165
20	37.649662237300625	29.545647713988053	17.809278206226356	14.995411842484966
21	31.262682323913232	32.50782124355101	22.33600581421092	13.893490618324842
22	29.08311664913344	24.170963452004514	31.109641950671836	15.63627794819021
23	37.81013323724413	19.14327927806881	27.193548994549687	15.853038490137369
24	42.67500525487813	15.244236047598763	21.331191684212616	20.74956701331049
25	32.83924755234647	15.050123648682007	20.634850351758743	31.475778447212775
26	23.627204440461085	16.333789858823184	28.710923682883244	31.328082017832486
27	18.77256225821835	18.84610510468786	25.880414751117264	36.50091788597653
28	21.192656301451365	28.99318570815654	20.103204279633825	29.710953710758275
29	32.33732398702999	26.208227535968557	19.885171370100686	21.56927710690076
30	32.540699221972666	19.52997723479915	26.278767594935164	21.650555948293018
31	39.3134609874285	17.204954497590393	23.88931216317249	19.59227235180862
32	45.27460491714088	16.33211033360969	22.036134221547698	16.357150527701734
33	41.73599797642659	17.40349473570635	26.304825683095984	14.555681604771072
34	27.162350541341514	22.041172797188167	36.415109415978186	14.38136724549213
35	22.112170908485727	35.24290260636866	29.279213941484322	13.365712543661292
36	22.30821730613317	42.52674898376001	20.97726991649706	14.187763793609761
37	32.409848939430724	36.71579532389644	15.755778711865235	15.118577024807605
38	32.06686953295457	36.66724177681553	15.488021677072089	15.777867013157806
39	35.75098443080127	28.32071399161561	14.870618029652272	21.05768354793085
40	27.51326952155416	25.01540837146619	15.51077160950937	31.96055049747028
41	20.323272977304523	30.389787265229096	16.454461200677308	32.83247855678908
42	18.321024449306584	25.29833202788419	17.973159151300283	38.40748437150894
43	19.27967708328562	20.167433395373976	21.588566199504182	38.96432332183622
44	24.728209559959303	18.50144617299822	20.00915086767835	36.761193399364124
45	28.22711863199109	24.400142301590815	19.183995040820097	28.188744025598
46	24.732281136234434	28.751792129744853	18.378230095972143	28.137696638048578
47	20.9600166120312	23.322192482750513	25.561915696995328	30.155875208222955
48	25.89283305875641	20.458601993749113	29.619190560457564	24.029374387036913
49	30.102639348425647	16.182479905498713	25.082843853523006	28.63203689255263
50	23.87002307056907	16.099674223003284	29.255140746757625	30.775161959670015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	18.0
6	34.0
7	81.0
8	128.0
9	290.5
10	453.0
11	692.5
12	932.0
13	1218.0
14	1504.0
15	1559.5
16	1615.0
17	2043.5
18	2472.0
19	2618.5
20	2765.0
21	3174.5
22	3584.0
23	5091.5
24	6599.0
25	6880.5
26	7162.0
27	9898.0
28	12634.0
29	13551.5
30	14469.0
31	17275.0
32	20081.0
33	24311.5
34	28542.0
35	27503.5
36	26465.0
37	29141.5
38	31818.0
39	34100.0
40	36382.0
41	40607.5
42	44833.0
43	47156.0
44	49479.0
45	52619.5
46	55760.0
47	57841.0
48	59922.0
49	65803.5
50	71685.0
51	80223.0
52	88761.0
53	102173.5
54	115586.0
55	156608.5
56	197631.0
57	224934.0
58	252237.0
59	272544.0
60	292851.0
61	302362.5
62	311874.0
63	210808.5
64	109743.0
65	81229.5
66	52716.0
67	37589.5
68	22463.0
69	20402.5
70	18342.0
71	14515.5
72	10689.0
73	8157.0
74	5625.0
75	5090.0
76	4555.0
77	3207.0
78	1859.0
79	1098.0
80	337.0
81	205.0
82	73.0
83	121.5
84	170.0
85	86.5
86	3.0
87	3.5
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1964841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.412044675378725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.94902304100292	5.968263514953912
2	10.411433200866654	1.751628824408232
3	4.001270618121288	1.009766015937496
4	1.8611753704453096	0.6262516145960197
5	1.0682618087728426	0.44931330301990186
6	0.6621463470788808	0.33420027919598233
7	0.4759738152797506	0.28027390989106066
8	0.4042181243688288	0.2720240736630706
9	0.3324933362999114	0.251725391929852
>10	6.000530660346507	13.405856225902104
>50	2.0219515100457706	11.694064739027473
>100	1.5736455191306449	29.6131254391395
>500	0.1585844321604526	9.2483968430369
>1k	0.06892323397742747	10.458865458028919
>5k	0.005489461113246436	3.0928211954520157
>10k+	0.004879520989552388	11.543423171817548
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	74898	3.8119114981822957	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	47488	2.416887676916351	No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	31362	1.5961596892572987	No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	28222	1.4363503204585002	No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	12163	0.6190322779298681	No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	10694	0.5442679585778187	No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	10110	0.5145454517693798	No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	10044	0.5111864013423987	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	8753	0.44548133920251054	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	8520	0.4336228733011985	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	8467	0.43092545401892574	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	7025	0.3575352916597323	No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	6249	0.3180410017909846	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	5480	0.2789029748463107	No Hit
TAGCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	5450	0.2773761337431375	No Hit
TAGCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5247	0.2670445089449986	No Hit
TAGCATGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5088	0.25895225109818043	No Hit
TAGCATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	4849	0.24678841697623372	No Hit
TAGCATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	4792	0.24388741888020457	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTC	4760	0.2422587883701531	No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT	4747	0.24159715722544473	No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	3710	0.18881934975908993	No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3615	0.18398435293237467	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	3141	0.1598602635022376	No Hit
TAGCATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC	2946	0.14993579633161155	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGC	2941	0.14968132281441604	No Hit
TAGCATGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	2910	0.14810358700780368	No Hit
TAGCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2883	0.1467294300149478	No Hit
TAGCATGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGG	2871	0.1461186935736785	No Hit
TAGCATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGC	2866	0.14586422005648295	No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCC	2817	0.14337037958796667	No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGC	2774	0.141181907340085	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGCGTGTAAGAGCACCCGACTGT	2652	0.13497275352051386	No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	2632	0.13395485945173172	No Hit
TAGCATGGGGGGGCAGGAGAGATGGCTCAGTGGATAAGAGCACAGACTGC	2628	0.13375128063797528	No Hit
TAGCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2614	0.13303875478982777	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCTGACTG	2566	0.1305958090247506	No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT	2561	0.13034133550755506	No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTGCT	2505	0.12749123211496502	No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2410	0.12265623528824977	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG	2272	0.11563276621365291	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTG	2261	0.11507292447582272	No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCG	2239	0.11395324100016235	No Hit
TAGCATGGGGGGCATGGTGGCACACGCCTTTAATACCAGCACTCAGTAGG	2148	0.10932182298720354	No Hit
TAGCATGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2135	0.10866019184249515	No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	2132	0.10850750773217781	No Hit
TAGCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2111	0.10743871895995656	No Hit
TAGCATGGGGAGCCGGGCGTGGTGGCGCATGCCTTTAATCCCAGCACTCG	2041	0.103876089719219	No Hit
TAGCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG	2035	0.10357072149858437	No Hit
TAGCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1993	0.10143314395414182	No Hit
TAGCATGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT	1969	0.10021167107160324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.143152550257247E-4	0.0	0.0	0.0	0.0
8	0.0013741569928559104	0.0	0.0	0.0	0.0
9	0.0017813146203687728	0.0	0.0	0.0	0.0
10	0.011145940053164606	0.0	0.0	0.0	0.0
11	0.024989299388601928	0.0	0.0	0.0	0.0
12	0.033641398973250254	0.0	0.0	0.0	0.0
13	0.040054131606577836	0.0	0.0	0.0	0.0
14	0.046924916570857386	0.0	0.0	0.0	0.0
15	0.05425375386608891	0.0	0.0	0.0	0.0
16	0.06346569518856741	0.0	0.0	0.0	0.0
17	0.07186332125602021	0.0	0.0	0.0	0.0
18	0.08250031427479373	0.0	0.0	0.0	0.0
19	0.10087330221631165	0.0	0.0	0.0	0.0
20	0.11537829269645737	0.0	0.0	0.0	0.0
21	0.12520097046020517	0.0	0.0	0.0	0.0
22	0.13344591241734063	0.0	0.0	0.0	0.0
23	0.14062206560225485	0.0	0.0	0.0	0.0
24	0.1488161128559512	0.0	0.0	0.0	0.0
25	0.15762089655091685	0.0	0.0	0.0	0.0
26	0.16622210143212607	0.0	0.0	0.0	0.0
27	0.17477241160989618	0.0	0.0	0.0	0.0
28	0.18632550929057365	0.0	0.0	0.0	0.0
29	0.1953847665027348	0.0	0.0	0.0	0.0
30	0.20378239257018763	0.0	0.0	0.0	0.0
31	0.21167107160324933	0.0	0.0	0.0	0.0
32	0.2199160135603848	0.0	0.0	0.0	0.0
33	0.22693948263498165	0.0	0.0	0.0	0.0
34	0.23615142395746017	0.0	0.0	0.0	0.0
35	0.2564584106296642	0.0	0.0	0.0	0.0
36	0.2840942345970997	0.0	0.0	0.0	0.0
37	0.3057753782621596	0.0	0.0	0.0	0.0
38	0.3231813668383345	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	30	2.528639E-6	44.0	25
TCGCATA	40	8.3182385E-9	44.0	23
ACACGTC	25	4.4439967E-5	44.0	32
CGAACGA	25	4.4439967E-5	44.0	10
GACGTTA	50	2.7284841E-11	44.0	9
CGCATTA	20	7.857848E-4	44.0	15
CGACAAC	20	7.857848E-4	44.0	40
CTCGGTT	50	2.7284841E-11	44.0	18
CGGATAC	20	7.857848E-4	44.0	32
TTGGTCG	20	7.857848E-4	44.0	22
GTATCGC	20	7.857848E-4	44.0	39
CGTACCG	25	4.4439967E-5	44.0	37
CGTAAAG	25	4.4439967E-5	44.0	12
TTACGCC	25	4.4439967E-5	44.0	30
CGATGCG	20	7.857848E-4	44.0	22
ACCGTAC	20	7.857848E-4	44.0	26
ACGTAGT	20	7.857848E-4	44.0	21
CGGTATG	20	7.857848E-4	44.0	14
CCACGTT	30	2.528639E-6	44.0	20
TATCGCC	20	7.857848E-4	44.0	40
>>END_MODULE