FastQCFastQC Report
Fri 17 Jun 2016
SRR1527491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564869
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC581573.716413322776539No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT322472.060683673841069No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT233941.4949494174911766No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC207711.3273315529926146No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA87730.5606220073373555No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT87280.5577463672678032No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT76520.48898661804917853No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT72380.46253072940929885No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC69820.4461715325691799No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC68260.4362026469947325No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT63940.4085965023270318No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC61270.3915343712476891No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC55100.3521061507384963No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC47490.3034758820067367No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT44470.2841771419844089No Hit
TAGCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT43110.27548631866309575No Hit
TAGCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA40920.2614915369912753No Hit
TAGCATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG39880.254845613274977No Hit
TAGCATGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT37800.24155376584238045No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT36630.23407710166154483No Hit
TAGCATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG35690.2280702090718137No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC35380.22608921257945552No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29790.1903673726043522No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT27690.17694771894644215No Hit
TAGCATGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGCA25760.16461441820369627No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC24970.15956607230381584No Hit
TAGCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC24570.15700994779754726No Hit
TAGCATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTC24240.15490114507987568No Hit
TAGCATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG24070.15381479216471156No Hit
TAGCATGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT23740.15170598944703997No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCAC23540.15042792719390569No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGCTC23240.14851083381420424No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC22850.14601861242059241No Hit
TAGCATGGGGGGGCAGGAGAGATGGCTCAGTGGATAAGAGCACAGACTGCTC21730.13886146380304037No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGCGTGTAAGAGCACCCGACTGTTC21610.1380946264511598No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT21490.13732778909927923No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT20640.1318960245234585No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC20530.13119309028423465No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTGCTCT20450.13068186538298096No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCTGACTGCT20030.12799793465139891No Hit
TAGCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT19750.12620864749701094No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT19080.12192713894901105No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT18650.11917930510477236No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCGCT18560.11860417709086192No Hit
TAGCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG17770.11355583119098149No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCT17750.11342802496566806No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT17270.11036067555814577No Hit
TAGCATGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC17120.10940212886829505No Hit
TAGCATGGGGGGCATGGTGGCACACGCCTTTAATACCAGCACTCAGTAGGCA16770.10716551992531005No Hit
TAGCATGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC16730.10690990747468318No Hit
TAGCATGGGGAGCCGGGCGTGGTGGCGCATGCCTTTAATCCCAGCACTCGGG16670.10652648879874291No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTT16600.10607916701014591No Hit
TAGCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC16360.10454549230638475No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCT16020.10237278647605647No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGCGTGTAGGAGCACCCGACTGATC15840.1012225304482356No Hit
TAGCATGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15830.10115862733557889No Hit
TAGCATGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT15700.10032788687104159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT351.0198892E-746.00000443
CGAGATA351.0198892E-746.00000418
GTACGGT351.0198892E-746.00000434
GATACGA206.312157E-446.09
CGAACGA206.312157E-446.010
GCGACGA206.312157E-446.036
CAACGAA253.417726E-546.013
GCGAATC253.417726E-546.043
CGACGAA453.110472E-1046.037
TCCGATA206.312157E-446.022
ATTAACG206.312157E-446.026
ATATGCG253.417726E-546.012
TACGGAT206.312157E-446.012
GCGCAAC253.417726E-546.016
CGTTAGC206.312157E-446.018
AACGGTA301.8618375E-646.045
TACGCGC206.312157E-446.015
TCGTAAT253.417726E-546.028
CGTGTCG253.417726E-546.030
GTATACG206.312157E-446.044
Produced by FastQC (version 0.11.5)