FastQCFastQC Report
Fri 17 Jun 2016
SRR1527490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100755
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC773843.6836280289705368No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT431132.0522621628890567No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT315881.5036498782580547No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC275881.313242143895885No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT119310.5679386696687619No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA108410.5160525620550707No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT105520.502295603247404No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC93150.4434120113959029No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT91770.43684294456040806No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC90660.43155912993185785No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT85170.40542566839065No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC84700.40318837751189457No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC75490.35934699667500497No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC66040.3143631694319423No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT60140.2862780286135223No Hit
TAGCATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT58480.27837610763749226No Hit
TAGCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA56620.26952214798965135No Hit
TAGCATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG52460.2497197436159857No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT50750.24157981297200293No Hit
TAGCATGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT49940.23772405635116897No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC49910.23758125055039736No Hit
TAGCATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG47410.22568076715276172No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG41510.1975956263343417No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT38000.1808873476440613No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC34500.16422667088737145No Hit
TAGCATGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC32290.15370664356386154No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGCTC31620.1505173140132952No Hit
TAGCATGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT31330.14913685793916948No Hit
TAGCATGGGGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGAGGCA31190.14847043086890188No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC30950.14732798446272888No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCAC30480.1450906935839734No Hit
TAGCATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG30130.1434246259083044No Hit
TAGCATGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTC30000.14280580077162736No Hit
TAGCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT29260.1392832576859272No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGCGTGTAAGAGCACCCGACTGTTC28840.13728397647512441No Hit
TAGCATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT28760.13690316100640007No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCTGACTGCT27600.13138133670989716No Hit
TAGCATGGGGGGGCAGGAGAGATGGCTCAGTGGATAAGAGCACAGACTGCTC27280.1298580748349998No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTACGAGCACTGACTGCTCT27170.12933445356550383No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC25940.12347941573386712No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT25370.12076610551920619No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT24920.11862401850763178No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCGCT24450.11638672762887628No Hit
TAGCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG23870.11362581548062481No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCT23300.11091250526596391No Hit
TAGCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC23020.10957965112542872No Hit
TAGCATGGGGGGCATGGTGGCACACGCCTTTAATACCAGCACTCAGTAGGCA23000.10948444725824764No Hit
TAGCATGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC22970.109341641457476No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT22910.10905602985593274No Hit
TAGCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTT22640.10777077764898811No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT22580.10748516604744485No Hit
TAGCATGGGGAGCCGGGCGTGGTGGCGCATGCCTTTAATCCCAGCACTCGGG21990.10467665196560284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG206.3126517E-446.00000422
ATATGCG351.02010745E-746.00000412
CTCGGTT351.02010745E-746.00000418
CGGGATA206.3126517E-446.00000430
CGTGTCG405.6152203E-946.00000430
TATAGCG206.3126517E-446.00000421
GACGCAT206.3126517E-446.00000427
CCGTTTA405.6152203E-946.00000430
ATCGCTT800.046.00000440
TCTATCG206.3126517E-446.00000442
TAATCGA405.6152203E-946.00000436
CAACGTT253.418128E-546.026
AACGGTA453.110472E-1046.045
CGGATAC253.418128E-546.032
CGATGCG301.8621449E-646.022
CAGTACG301.8621449E-646.040
TAGTGCG253.418128E-546.029
ACGGTAA453.110472E-1046.046
ATGGGGG903250.045.4779975
TAGCATG2117300.045.3873331
Produced by FastQC (version 0.11.5)