FastQCFastQC Report
Fri 17 Jun 2016
SRR1527489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745150
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC191922.5755888076226263No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG93761.2582701469502784No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC70460.945581426558411No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT52150.6998590887740723No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC37260.5000335502918876No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT28560.38327853452325034No Hit
CCGCTAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG24480.328524458162786No Hit
CCGCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAG18980.25471381601019927No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT18630.2500167751459438No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA18380.24666174595718982No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC17380.23324162920217406No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG16990.2280077836677179No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT13120.17607193182580688No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA12560.16855666644299805No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT12350.16573844192444473No Hit
CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC11650.1563443601959337No Hit
CCGCTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC10540.1414480305978662No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG10450.1402402200899148No Hit
CCGCTAGGGAGGGACAGAGAGAGAGATGGGGACCGGGAGGATCGATTCAG10180.13661678856606052No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC10110.13567738039320942No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC9850.13218815003690532No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC9780.13124874186405422No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT9650.12950412668590217No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT9430.12655170099979868No Hit
CCGCTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA9290.12467288465409648No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCAACTGCT9240.12400187881634571No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA8870.11903643561698987No Hit
CCGCTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT8800.11809702744413877No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC8590.11527880292558546No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTG8520.11433939475273434No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG8520.11433939475273434No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG8490.11393679125008388No Hit
CCGCTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA8390.11259477957458229No Hit
CCGCTAGGGAGTCTAATTCTACTAAAGGCTTAACCATTCTTCTCTTCACT8380.11246057840703214No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG8350.11205797490438166No Hit
CCGCTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG8190.10991075622357915No Hit
CCGCTAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA7930.10642152586727505No Hit
CCGCTAGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT7790.10454270952157284No Hit
CCGCTAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA7790.10454270952157284No Hit
CCGCTAGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGC7680.10306649667852111No Hit
CCGCTAGGGGAAAATGGAGAAATATTTTCAATGAACTCTCATTTCCAAAA7590.10185868617056967No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC7500.10065087566261827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG207.8546983E-444.031
AGCGTTG207.8546983E-444.031
ACTATCC207.8546983E-444.025
TCGTTAT207.8546983E-444.036
GCGACTA302.5265144E-644.09
CCGATTT302.5265144E-644.032
CGAAAAT351.4451689E-744.017
CGACGGA351.4451689E-744.029
TCCGACG351.4451689E-744.027
TAGGTCG207.8546983E-444.035
CTATACT302.5265144E-644.013
CGCGCAT207.8546983E-444.014
TTCGTAC207.8546983E-444.021
TTACTAC254.441328E-544.019
CGCGATT408.305506E-944.028
TTATCCG950.044.016
GTAATTA600.044.011
CGTTATC207.8546983E-444.042
TGGTTCG302.5265144E-644.020
AACGGTA502.7284841E-1144.034
Produced by FastQC (version 0.11.5)