FastQCFastQC Report
Fri 17 Jun 2016
SRR1527488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595776
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC153412.574961059190031No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT68841.1554678268342464No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA60351.0129646041465248No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41900.7032844559028897No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT30520.5122730690729401No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG23210.3895759480073048No Hit
CCGCTAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA20130.33787866580728326No Hit
CCGCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA17150.2878598667955742No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT15210.2552972929423139No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC15010.25194032656568915No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA13650.22911295520464067No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC12600.2114888817273606No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA11250.18882935868514342No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT10380.17422655494682565No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA9890.16600198732409496No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC9620.1614700827156515No Hit
CCGCTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC9580.16079868944032658No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCAACTGCTCT9420.15811311633902675No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT9070.15223842517993338No Hit
CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT8670.14552449242668386No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT8560.14367816091954022No Hit
CCGCTAGGGAGGGACAGAGAGAGAGATGGGGACCGGGAGGATCGATTCAGAA8520.14300676764421527No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCGGCGGTTAAGAGCACTGACTGCTC8500.1426710710065528No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC8280.13897840799226555No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC8170.13713207648512193No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC8070.13545359329680953No Hit
CCGCTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA7920.13293586851434094No Hit
CCGCTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT7770.13041814373187238No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC7570.1270611773552476No Hit
CCGCTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA7280.12219357610914168No Hit
CCGCTAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA7040.11816521645719197No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG7020.11782951981952948No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT6900.11581533999355462No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT6810.11430470512407348No Hit
CCGCTAGGGAGTCTAATTCTACTAAAGGCTTAACCATTCTTCTCTTCACTTT6780.11380116016757977No Hit
CCGCTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA6750.11329761521108606No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT6590.11061204210978623No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCT6500.10910140724030508No Hit
CCGCTAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAA6460.10843001396498013No Hit
CCGCTAGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTC6390.10725507573316145No Hit
CCGCTAGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTC6340.10641583413900527No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC6220.1044016543130304No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC6030.10121253625523688No Hit
CCGCTAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC5980.10037329466108066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA551.8189894E-1246.00000442
AGGCCGT351.0184522E-746.00000430
AATTCCG351.0184522E-746.00000429
CGCCAGT351.0184522E-746.00000416
ACGCGAT301.8598839E-646.027
AACGTAC405.6043064E-946.027
TAATAGC405.6043064E-946.035
AACAACG301.8598839E-646.022
AGGTACG253.4151602E-546.026
ACACGGG253.4151602E-546.030
CGAACCT405.6043064E-946.029
CTACTAG206.3089945E-446.022
CGAAACA206.3089945E-446.015
TCCGATT206.3089945E-446.019
ACCGCGA301.8598839E-646.033
CTATACG206.3089945E-446.041
CGTTGAC301.8598839E-646.029
GGGTAAT206.3089945E-446.07
TATATTC253.4151602E-546.019
CGATTTG206.3089945E-446.039
Produced by FastQC (version 0.11.5)