##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527488_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595776
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23874744870555	33.0	31.0	34.0	31.0	34.0
2	32.83524009023525	34.0	31.0	34.0	31.0	34.0
3	32.97167391771404	34.0	31.0	34.0	31.0	34.0
4	36.48354582930497	37.0	37.0	37.0	35.0	37.0
5	36.41305792781179	37.0	37.0	37.0	35.0	37.0
6	35.33371938446665	37.0	35.0	37.0	32.0	37.0
7	36.26753343538511	37.0	35.0	37.0	35.0	37.0
8	36.656025083252764	37.0	37.0	37.0	35.0	37.0
9	38.76036463368783	39.0	39.0	39.0	39.0	39.0
10	38.026855731013	39.0	39.0	39.0	35.0	39.0
11	37.80825679449995	39.0	37.0	39.0	35.0	39.0
12	37.18420681598453	39.0	35.0	39.0	35.0	39.0
13	37.0895856832098	39.0	35.0	39.0	35.0	39.0
14	37.636427113546034	40.0	36.0	40.0	33.0	40.0
15	37.74713315071436	40.0	36.0	40.0	34.0	40.0
16	37.925309176603285	40.0	36.0	40.0	35.0	40.0
17	37.78679067300462	40.0	36.0	40.0	34.0	40.0
18	37.71358698571275	40.0	36.0	40.0	34.0	40.0
19	37.58365895907187	40.0	36.0	40.0	33.0	40.0
20	37.48248167096359	40.0	35.0	40.0	33.0	40.0
21	37.457337992802664	40.0	35.0	40.0	33.0	40.0
22	37.49586925287356	40.0	35.0	40.0	33.0	40.0
23	37.44723855945859	40.0	35.0	40.0	34.0	40.0
24	37.42782186593619	39.0	35.0	40.0	34.0	40.0
25	37.511799736813835	39.0	35.0	40.0	34.0	40.0
26	37.46538631969062	39.0	35.0	40.0	34.0	40.0
27	37.44182712965947	39.0	35.0	40.0	34.0	40.0
28	37.27071583951015	39.0	35.0	40.0	33.0	40.0
29	37.19027285422709	39.0	35.0	40.0	33.0	40.0
30	37.007440715973786	39.0	35.0	40.0	33.0	40.0
31	36.92847143893007	39.0	35.0	40.0	32.0	40.0
32	36.89728522129122	39.0	35.0	40.0	33.0	40.0
33	36.829341900311526	39.0	35.0	40.0	33.0	40.0
34	36.18752014179826	38.0	35.0	40.0	30.0	40.0
35	36.036498616929855	38.0	35.0	40.0	30.0	40.0
36	36.075086945429156	38.0	35.0	40.0	31.0	40.0
37	36.05005404715866	38.0	35.0	40.0	31.0	40.0
38	36.122955607476634	38.0	35.0	40.0	31.0	40.0
39	36.119096774626705	38.0	35.0	40.0	31.0	40.0
40	35.908697228488556	38.0	35.0	40.0	30.0	40.0
41	35.74187446288538	38.0	35.0	40.0	30.0	40.0
42	35.664518208185626	37.0	35.0	40.0	30.0	40.0
43	35.67925529057901	37.0	35.0	40.0	30.0	40.0
44	35.58702431786443	37.0	35.0	40.0	30.0	40.0
45	35.418140374906	37.0	34.0	40.0	30.0	40.0
46	35.24468088677624	36.0	34.0	40.0	29.0	40.0
47	35.11460851058116	36.0	34.0	40.0	29.0	40.0
48	34.853144470404985	36.0	34.0	40.0	28.0	40.0
49	34.75968820496294	35.0	34.0	40.0	28.0	40.0
50	35.19224003652379	35.0	34.0	40.0	30.0	40.0
51	35.25633795251907	35.0	35.0	40.0	30.0	40.0
52	34.9439772666237	35.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	3.0
13	5.0
14	7.0
15	14.0
16	45.0
17	82.0
18	126.0
19	222.0
20	362.0
21	515.0
22	786.0
23	1164.0
24	1611.0
25	2193.0
26	2980.0
27	3926.0
28	5131.0
29	6915.0
30	9349.0
31	13054.0
32	18662.0
33	25921.0
34	62845.0
35	48888.0
36	55952.0
37	92254.0
38	187037.0
39	55725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.52213583628746	0.41240331936835317	0.03071624234611666	0.034744601998066386
8	99.73228193146417	0.18379390912020624	0.0735175636480825	0.010406595767536792
9	99.55755183156086	0.19336126329358685	0.09617708669029972	0.15290981845525836
10	69.58739526264905	23.554490278225373	1.7681141905682671	5.09000026855731
11	44.050448490707915	23.192105757868728	15.83632103340853	16.921124718014823
12	38.46798125469975	14.257371898163068	23.494232731764956	23.78041411537222
13	28.34018154474165	14.639226823504137	26.79698410140724	30.223607530346975
14	22.619239445697712	16.834682833816736	33.15440702545923	27.39167069502632
15	27.494226017832208	18.38811902459985	28.715154689010635	25.40250026855731
16	36.461522451391126	19.103320711139755	23.06638736706413	21.368769470404985
17	35.414484638521856	17.862585938339244	23.40762299924804	23.31530642389086
18	32.178704748093246	18.363109625093994	24.34723788806531	25.11094773874745
19	30.670755451713394	23.53770544634225	21.63665538725964	24.154883714684715
20	31.304718551938983	23.969243474057365	21.184975561284777	23.541062412718876
21	31.860296487270386	22.520544634224944	23.02526452895048	22.593894349554194
22	27.955808894618112	19.982510205177782	28.675374637447632	23.386306262756474
23	31.154326458266198	19.785624127188743	26.46346949188957	22.596579922655494
24	33.68178644322698	20.6225158448813	22.88074712643678	22.814950585454934
25	30.019503974648188	21.28283113116339	20.192488452035665	28.50517644215276
26	27.055302664088515	20.587099849607906	24.56510500590826	27.792492480395314
27	24.23142254807176	17.740895907186594	25.174562251584486	32.853119293157164
28	26.263226447523902	23.146618863465463	20.42344773874745	30.166706950263183
29	30.70113599742185	23.49809324309808	20.081708561607048	25.719062197873026
30	30.381888495004834	19.973110699323236	24.956359437103877	24.68864136856805
31	32.54427838650768	20.536913202277365	23.142254807175853	23.7765536040391
32	36.0143745300247	19.485511333118488	21.645047803201205	22.855066333655603
33	36.30525566655925	17.96396632291331	21.749449457514235	23.981328553013213
34	29.34391449135245	20.850789558491783	27.930631646793426	21.874664303362337
35	26.753175690192286	26.932941239660547	26.380888119024597	19.93299495112257
36	28.66849285637555	29.102884305510795	21.112800784187346	21.11582205392631
37	31.59610323343001	27.206366151036633	19.596794768503596	21.600735847029757
38	30.684686862176385	28.089248308088948	19.87357664625631	21.352488183478354
39	33.076189708883874	23.049434686862178	19.072100923837144	24.8022746804168
40	29.56580996884735	22.40607208078204	19.938533945644	28.08958400472661
41	23.999624019765818	25.844109195402297	21.181786443226986	28.974480341604895
42	24.077673488022345	22.71222741433022	21.8884278655065	31.32167123214094
43	23.706728703405304	20.478669835642926	24.610424051992695	31.204177408959072
44	26.399351434096037	19.482490063379526	22.941004672897197	31.17715382962724
45	29.18345149854979	19.919902782253732	22.28723547104952	28.609410248146954
46	27.55280508110431	23.68675475346439	20.555376517348805	28.2050636480825
47	24.64332232248362	22.195254592330002	24.93051079600387	28.230912289182513
48	26.763582285959824	20.759312224728756	26.301160167579763	26.175945321731657
49	30.46144859813084	19.255894832957352	23.703371737028682	26.579284831883125
50	25.84796970673542	19.655877376732196	24.744702707057687	29.7514502094747
51	23.9742789236223	20.89946557095284	26.425535771833708	28.700719733591146
52	23.178677892362227	22.485464335589214	26.12173031474917	28.214127457299387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	39.5
14	161.0
15	244.0
16	482.0
17	720.0
18	679.0
19	638.0
20	699.5
21	761.0
22	737.5
23	714.0
24	843.5
25	973.0
26	1060.5
27	1148.0
28	1467.5
29	1787.0
30	2007.0
31	2227.0
32	2684.0
33	3141.0
34	3478.5
35	3816.0
36	4783.0
37	5750.0
38	7184.0
39	8481.0
40	8344.0
41	9282.0
42	10220.0
43	11640.0
44	13060.0
45	13225.5
46	13391.0
47	15198.5
48	17006.0
49	18006.5
50	19007.0
51	19865.5
52	20724.0
53	23209.0
54	25694.0
55	27849.0
56	30004.0
57	31923.0
58	33842.0
59	42794.0
60	51746.0
61	53319.5
62	54893.0
63	55365.5
64	63601.0
65	71364.0
66	56919.0
67	42474.0
68	35392.5
69	28311.0
70	24521.0
71	20731.0
72	17392.5
73	14054.0
74	13405.0
75	12756.0
76	11963.0
77	11170.0
78	8556.5
79	5943.0
80	4509.5
81	3076.0
82	1934.5
83	793.0
84	667.5
85	542.0
86	341.0
87	140.0
88	81.5
89	17.0
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	595776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.880223439682029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.54552867627072	6.673649156730047
2	9.564419679260668	1.8899720700397464
3	3.2991301984234847	0.9778843055107961
4	1.6444686055993478	0.6499086905145558
5	0.8731992389236205	0.4313701793962832
6	0.7186055993476488	0.42599903319368354
7	0.5521201413427562	0.3818549253410678
8	0.47057624354444144	0.3719518745300247
9	0.4145148138081	0.3685949081533999
>10	10.666961130742049	28.097976420668168
>50	2.675659146507203	17.97068025566656
>100	1.4694889915737972	27.967390428617467
>500	0.08154389779831477	5.507606885809432
>1k	0.01868714324544713	3.5417673756579657
>5k	0.003397662408263115	2.168432430980771
>10k+	0.0016988312041315574	2.574961059190031
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	15341	2.574961059190031	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6884	1.1554678268342464	No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6035	1.0129646041465248	No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4190	0.7032844559028897	No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3052	0.5122730690729401	No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2321	0.3895759480073048	No Hit
CCGCTAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	2013	0.33787866580728326	No Hit
CCGCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	1715	0.2878598667955742	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	1521	0.2552972929423139	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1501	0.25194032656568915	No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1365	0.22911295520464067	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1260	0.2114888817273606	No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1125	0.18882935868514342	No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1038	0.17422655494682565	No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	989	0.16600198732409496	No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	962	0.1614700827156515	No Hit
CCGCTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	958	0.16079868944032658	No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCAACTGCTCT	942	0.15811311633902675	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	907	0.15223842517993338	No Hit
CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	867	0.14552449242668386	No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	856	0.14367816091954022	No Hit
CCGCTAGGGAGGGACAGAGAGAGAGATGGGGACCGGGAGGATCGATTCAGAA	852	0.14300676764421527	No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCGGCGGTTAAGAGCACTGACTGCTC	850	0.1426710710065528	No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	828	0.13897840799226555	No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	817	0.13713207648512193	No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	807	0.13545359329680953	No Hit
CCGCTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	792	0.13293586851434094	No Hit
CCGCTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	777	0.13041814373187238	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	757	0.1270611773552476	No Hit
CCGCTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	728	0.12219357610914168	No Hit
CCGCTAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	704	0.11816521645719197	No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	702	0.11782951981952948	No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	690	0.11581533999355462	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	681	0.11430470512407348	No Hit
CCGCTAGGGAGTCTAATTCTACTAAAGGCTTAACCATTCTTCTCTTCACTTT	678	0.11380116016757977	No Hit
CCGCTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	675	0.11329761521108606	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	659	0.11061204210978623	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCT	650	0.10910140724030508	No Hit
CCGCTAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAA	646	0.10843001396498013	No Hit
CCGCTAGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTC	639	0.10725507573316145	No Hit
CCGCTAGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTC	634	0.10641583413900527	No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	622	0.1044016543130304	No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	603	0.10121253625523688	No Hit
CCGCTAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	598	0.10037329466108066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.02484155118702331	0.0	0.0	0.0	0.0
7	0.025177247824685788	0.0	0.0	0.0	0.0
8	0.025345096143517026	0.0	0.0	0.0	0.0
9	0.025512944462348267	0.0	0.0	0.0	0.0
10	0.032730422172091524	0.0	0.0	0.0	0.0
11	0.060089698141583416	0.0	0.0	0.0	0.0
12	0.06898565903963906	0.0	0.0	0.0	0.0
13	0.07586744011171984	0.0	0.0	0.0	0.0
14	0.08425985605328178	0.0	0.0	0.0	0.0
15	0.10960495219679879	0.0	0.0	0.0	0.0
16	0.1226971210656354	0.0	0.0	0.0	0.0
17	0.12823611558706627	0.0	0.0	0.0	0.0
18	0.13629283489096572	0.0	0.0	0.0	0.0
19	0.15290981845525836	0.0	0.0	0.0	0.0
20	0.16348426254162637	0.0	0.0	0.0	0.0
21	0.1696946503383822	0.0	0.0	0.0	0.0
22	0.1757371898163068	0.0	0.0	0.0	0.0
23	0.18110833601890644	0.0	0.0	0.0	0.0
24	0.19000429691696208	0.0	0.0	0.0	0.0
25	0.19671822967021163	0.0	0.0	0.0	0.0
26	0.20494279729294232	0.0	0.0	0.0	0.0
27	0.21383875819099796	0.0	0.0	0.0	0.0
28	0.22458105059619723	0.0	0.0	0.0	0.0
29	0.23398055645074659	0.0	0.0	0.0	0.0
30	0.24338006230529596	0.0	0.0	0.0	0.0
31	0.2541223547104952	0.0	0.0	0.0	0.0
32	0.26503249543452573	0.0	0.0	0.0	0.0
33	0.2729213664195939	0.0	0.0	0.0	0.0
34	0.28299226554946827	0.0	0.0	0.0	0.0
35	0.2974272209689548	0.0	0.0	0.0	0.0
36	0.3145477494897411	0.0	0.0	0.0	0.0
37	0.3293184015468901	0.0	0.0	0.0	0.0
38	0.34123563218390807	0.0	0.0	0.0	0.0
39	0.35046728971962615	0.0	0.0	0.0	0.0
40	0.35567058760339454	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	55	1.8189894E-12	46.000004	42
AGGCCGT	35	1.0184522E-7	46.000004	30
AATTCCG	35	1.0184522E-7	46.000004	29
CGCCAGT	35	1.0184522E-7	46.000004	16
ACGCGAT	30	1.8598839E-6	46.0	27
AACGTAC	40	5.6043064E-9	46.0	27
TAATAGC	40	5.6043064E-9	46.0	35
AACAACG	30	1.8598839E-6	46.0	22
AGGTACG	25	3.4151602E-5	46.0	26
ACACGGG	25	3.4151602E-5	46.0	30
CGAACCT	40	5.6043064E-9	46.0	29
CTACTAG	20	6.3089945E-4	46.0	22
CGAAACA	20	6.3089945E-4	46.0	15
TCCGATT	20	6.3089945E-4	46.0	19
ACCGCGA	30	1.8598839E-6	46.0	33
CTATACG	20	6.3089945E-4	46.0	41
CGTTGAC	30	1.8598839E-6	46.0	29
GGGTAAT	20	6.3089945E-4	46.0	7
TATATTC	25	3.4151602E-5	46.0	19
CGATTTG	20	6.3089945E-4	46.0	39
>>END_MODULE