FastQCFastQC Report
Fri 17 Jun 2016
SRR1527487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771121
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC193262.5062214620014243No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT88651.1496250264225718No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA78131.0132002629937453No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC51420.6668214197253091No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT40340.5231345015892447No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29520.3828192981386838No Hit
CCGCTAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA26340.3415806339082971No Hit
CCGCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA21220.27518379087069345No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA18240.23653875332146318No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC18050.23407480797436458No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT17720.22979532395045657No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC17470.22655329059901105No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC14070.18246163701935234No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA12670.16430625025125759No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT12350.16015644756140734No Hit
CCGCTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC12010.15574728220344147No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT11810.1531536555222851No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA11580.15017098483895525No Hit
CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT11350.1471883141556254No Hit
CCGCTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA11240.14576181948098937No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC11010.14277914879765952No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC10560.13694348876505763No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT10450.13551699409042162No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC10440.1353873127563638No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCAACTGCTCT9870.12799547671506806No Hit
CCGCTAGGGAGTCTAATTCTACTAAAGGCTTAACCATTCTTCTCTTCACTTT9740.1263096193723164No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCT9560.12397535535927566No Hit
CCGCTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA9320.12086300334188799No Hit
CCGCTAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA9270.1202145966715989No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9250.11995523400348324No Hit
CCGCTAGGGAGGGACAGAGAGAGAGATGGGGACCGGGAGGATCGATTCAGAA9200.11930682733319413No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT9180.1190474646650785No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCGGCGGTTAAGAGCACTGACTGCTC9030.11710224465421122No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT8930.11580543131363302No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC8920.1156757499795752No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC8910.11554606864551736No Hit
CCGCTAGGGGAAAATGGAGAAATATTTTCAATGAACTCTCATTTCCAAAAGC8860.11489766197522829No Hit
CCGCTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA8400.10893232060856857No Hit
CCGCTAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA8350.10828391393827948No Hit
CCGCTAGGGGCAGCGAGTATCTGCCCTGCGTGCTGCCACCTGCTGTGTACGT8260.10711678193175908No Hit
CCGCTAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAA8250.10698710059770128No Hit
CCGCTAGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTC8200.10633869392741217No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG8100.10504188058683397No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT7930.10283729790785104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTA351.0189797E-746.00000434
CGGTATA351.0189797E-746.00000436
AACGTCG301.8606006E-646.032
ACCGGAT253.4161025E-546.042
CGAACAA206.310156E-446.035
CGTATCG206.310156E-446.013
CGACGTT206.310156E-446.026
CGACGTA206.310156E-446.014
GCGAATA253.4161025E-546.011
TCCGACG405.6079443E-946.027
TTAGATA253.4161025E-546.046
TAGCGAC850.046.030
ATTACCG206.310156E-446.029
TCGGGTA600.046.042
TTACTAC206.310156E-446.019
AAGGTCG206.310156E-446.032
TAACGTT253.4161025E-546.039
AAGCGAT253.4161025E-546.022
TAACGCC206.310156E-446.022
TAACGAG206.310156E-446.031
Produced by FastQC (version 0.11.5)