##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	771121
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.040692705814003	31.0	31.0	33.0	30.0	34.0
2	31.86594321773107	33.0	31.0	34.0	30.0	34.0
3	32.19548553339878	34.0	31.0	34.0	30.0	34.0
4	36.017220384349535	37.0	35.0	37.0	35.0	37.0
5	35.94950208851788	37.0	35.0	37.0	35.0	37.0
6	34.27110401610123	35.0	35.0	37.0	31.0	37.0
7	35.865573625929	37.0	35.0	37.0	35.0	37.0
8	36.449183720842775	37.0	37.0	37.0	35.0	37.0
9	38.623411889962796	39.0	39.0	39.0	37.0	39.0
10	37.532708874482736	39.0	37.0	39.0	35.0	39.0
11	37.11552921007209	39.0	37.0	39.0	34.0	39.0
12	36.4497724740994	38.0	35.0	39.0	33.0	39.0
13	36.27115199819484	38.0	35.0	39.0	32.0	39.0
14	36.81901543337557	39.0	35.0	40.0	32.0	40.0
15	36.93784762702611	39.0	35.0	40.0	32.0	40.0
16	37.094230347766434	39.0	35.0	40.0	33.0	40.0
17	36.98414126965807	39.0	35.0	40.0	32.0	40.0
18	36.959343604959535	39.0	35.0	40.0	32.0	40.0
19	36.802661320337535	39.0	35.0	40.0	31.0	40.0
20	36.62564240890859	39.0	35.0	40.0	31.0	40.0
21	36.65978491053933	39.0	35.0	40.0	31.0	40.0
22	36.72329245345413	39.0	35.0	40.0	31.0	40.0
23	36.74465745324015	39.0	35.0	40.0	31.0	40.0
24	36.78871928011298	39.0	35.0	40.0	32.0	40.0
25	36.83440082684819	38.0	35.0	40.0	32.0	40.0
26	36.738334191391495	38.0	35.0	40.0	32.0	40.0
27	36.72150414785747	38.0	35.0	40.0	32.0	40.0
28	36.5234846411912	38.0	35.0	40.0	31.0	40.0
29	36.41414901163371	38.0	35.0	40.0	31.0	40.0
30	36.323114011938465	38.0	35.0	40.0	31.0	40.0
31	36.20751088350596	38.0	35.0	40.0	31.0	40.0
32	36.169745085401644	38.0	35.0	40.0	31.0	40.0
33	36.0703339683396	38.0	35.0	40.0	31.0	40.0
34	35.453860029748895	38.0	34.0	40.0	28.0	40.0
35	35.308515784163575	38.0	34.0	40.0	28.0	40.0
36	35.45718505915414	37.0	34.0	40.0	29.0	40.0
37	35.50307150239716	37.0	34.0	40.0	30.0	40.0
38	35.40786465418527	37.0	34.0	40.0	29.0	40.0
39	35.32702260734697	37.0	34.0	40.0	29.0	40.0
40	35.52215281389043	37.0	34.0	40.0	30.0	40.0
41	35.56426553031236	37.0	34.0	40.0	30.0	40.0
42	35.61237082118111	37.0	34.0	40.0	30.0	40.0
43	35.58740197712162	37.0	34.0	40.0	30.0	40.0
44	35.51167974935192	37.0	34.0	40.0	30.0	40.0
45	35.38872628290502	36.0	34.0	40.0	30.0	40.0
46	35.17751299731171	36.0	34.0	40.0	29.0	40.0
47	35.07932996248319	36.0	34.0	40.0	29.0	40.0
48	34.93136096669654	35.0	34.0	40.0	29.0	40.0
49	34.81758245463423	35.0	34.0	39.0	29.0	40.0
50	34.742122183159324	35.0	34.0	39.0	29.0	40.0
51	34.58069874896417	35.0	34.0	39.0	28.0	40.0
52	34.18524330163489	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	8.0
14	11.0
15	19.0
16	60.0
17	98.0
18	198.0
19	334.0
20	613.0
21	1007.0
22	1566.0
23	2163.0
24	3156.0
25	4384.0
26	5614.0
27	7676.0
28	9951.0
29	13054.0
30	17540.0
31	22815.0
32	31355.0
33	45414.0
34	86027.0
35	63143.0
36	82985.0
37	128237.0
38	206942.0
39	36746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.54429979212081	0.40356831158793494	0.029437662831125075	0.02269423346011845
8	99.74725107992131	0.1755885263142879	0.06133927100934873	0.015821122755054005
9	99.5745155429563	0.18868634105412768	0.08701617515279703	0.14978194083678176
10	69.54343092718264	23.69303909503178	1.729430270995084	5.034099706790504
11	44.007749756523296	23.146302590644012	15.982446334621933	16.863501318210762
12	38.1429114237584	14.464007594138922	23.607708777221735	23.785372204880947
13	28.133587335839643	14.725704526267602	26.853373206020848	30.28733493187191
14	22.526166451179517	16.968802561465708	32.97900070157602	27.52603028577876
15	27.43966251729625	18.342516933140192	28.71287385507592	25.504946694487636
16	36.18614977415996	19.13409179622913	23.038278039373846	21.64148039023707
17	35.47873809687455	17.941542248233418	23.333562437023502	23.246157217868532
18	32.03012238027495	18.4802385099096	24.37633004418243	25.113309065633022
19	30.539954170616546	23.426414272208902	21.705542969261636	24.328088587912923
20	31.03079802002539	24.10996458402767	21.13053593404926	23.72870146189768
21	31.584926360454457	22.472867422881755	23.159011361381676	22.783194855282115
22	27.989122329699228	19.889096523113754	28.588120411712296	23.53366073547472
23	31.03079802002539	19.649315736440844	26.59323244990086	22.726653793632906
24	33.317468983466924	20.871691991269852	22.9089857493182	22.90185327594502
25	29.93032221921073	21.300937207001237	20.345834181665392	28.422906392122638
26	26.970086406672884	20.645787107341132	24.38644518823894	27.997681297747047
27	24.179214416414542	17.849987226388595	25.109937350947515	32.860861006249344
28	26.344244288509845	23.16782969209761	20.397577033954466	30.09034898543808
29	30.66730124066132	23.561671903631208	20.137047233832302	25.633979621875163
30	30.31132597867261	20.02954140789837	24.80453780924135	24.85459480418767
31	32.42020383312087	20.69221302493383	23.148896217325166	23.73868692462013
32	36.0095237971732	19.66137610050822	21.521654837567645	22.80744526475093
33	36.0110799731819	18.107015630491194	21.845858172712195	24.03604622361471
34	29.264797612826005	20.92602847024008	27.882524273103705	21.926649643830213
35	26.67285678901236	27.0499701084525	26.228438857196213	20.048734245338927
36	28.88249704002355	28.91219406552279	20.962339243776267	21.24296965067739
37	31.5168436600741	27.09458048736839	19.677326904597333	21.711248947960176
38	30.730715413015595	27.999237473755738	19.85732459626959	21.412722516959075
39	32.856062796889205	23.118032059819406	19.182203571164578	24.843701572126815
40	29.473454879325033	22.455490124118004	19.931242956682542	28.139812039874418
41	23.81973775840627	25.96246244104362	21.231428011946242	28.986371788603865
42	23.967704160566242	22.783843261952406	21.95543889999105	31.293013677490304
43	23.730257637906373	20.530630082697787	24.735936383524766	31.003175895871077
44	26.58778583387043	19.66565558453213	22.84907297298349	30.89748560861395
45	29.086615459830554	20.03433961725851	22.28988706052617	28.589157862384763
46	27.7042124387742	23.54131193418413	20.681968199543263	28.072507427498405
47	24.4910980248236	22.404395678499224	25.01825264776864	28.08625364890854
48	26.580004953826965	20.93977469165021	26.269418158758484	26.210802195764348
49	30.516741211820193	19.258585876924634	23.641425924076767	26.583246987178406
50	25.737465326453307	19.845393913536267	24.696253895303073	29.720886864707353
51	23.83750410117219	21.01161815071824	26.47794574392346	28.672932004186112
52	23.198434486935255	22.557808696689623	26.00655409462328	28.237202721751842
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	2.0
10	3.0
11	3.0
12	3.0
13	44.5
14	177.0
15	268.0
16	477.5
17	687.0
18	759.0
19	831.0
20	893.0
21	955.0
22	901.5
23	848.0
24	1077.0
25	1306.0
26	1377.0
27	1448.0
28	1881.5
29	2315.0
30	2628.0
31	2941.0
32	3529.0
33	4117.0
34	4477.0
35	4837.0
36	6229.5
37	7622.0
38	9441.0
39	11268.5
40	11277.0
41	12408.0
42	13539.0
43	15161.5
44	16784.0
45	17257.5
46	17731.0
47	19998.0
48	22265.0
49	23567.0
50	24869.0
51	26345.5
52	27822.0
53	30706.5
54	33591.0
55	36360.5
56	39130.0
57	41411.5
58	43693.0
59	54946.5
60	66200.0
61	68218.0
62	70236.0
63	71348.5
64	82197.5
65	91934.0
66	72899.0
67	53864.0
68	45211.5
69	36559.0
70	31563.0
71	26567.0
72	22439.0
73	18311.0
74	17452.0
75	16593.0
76	15730.0
77	14867.0
78	11162.0
79	7457.0
80	5709.0
81	3961.0
82	2536.0
83	1111.0
84	835.5
85	560.0
86	370.0
87	180.0
88	100.5
89	14.5
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	771121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.49358142237081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.57804581529089	6.605448431569105
2	9.686232190910706	1.8391406796079992
3	3.7796931987378253	1.0764847540139615
4	1.6924611034463917	0.6427006915905545
5	0.9671206305407952	0.4590719225646818
6	0.5832775554250277	0.3322435778561341
7	0.46443646099416724	0.3086415750576109
8	0.36745120002185583	0.27907423089242805
9	0.2991517204638901	0.2556019094279627
>10	7.624953897851299	20.286958856003142
>50	3.184121736992364	20.881677453992303
>100	1.6364555302088597	30.708410223557653
>500	0.10381520892810796	6.729423786928381
>1k	0.027319791823186307	4.25925373579503
>5k	0.004097968773477946	2.829646709141626
>10k+	0.0013659895911593155	2.5062214620014243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	19326	2.5062214620014243	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8865	1.1496250264225718	No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7813	1.0132002629937453	No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5142	0.6668214197253091	No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4034	0.5231345015892447	No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2952	0.3828192981386838	No Hit
CCGCTAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	2634	0.3415806339082971	No Hit
CCGCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2122	0.27518379087069345	No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1824	0.23653875332146318	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1805	0.23407480797436458	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	1772	0.22979532395045657	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1747	0.22655329059901105	No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1407	0.18246163701935234	No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1267	0.16430625025125759	No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1235	0.16015644756140734	No Hit
CCGCTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1201	0.15574728220344147	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	1181	0.1531536555222851	No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1158	0.15017098483895525	No Hit
CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1135	0.1471883141556254	No Hit
CCGCTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1124	0.14576181948098937	No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1101	0.14277914879765952	No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1056	0.13694348876505763	No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1045	0.13551699409042162	No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	1044	0.1353873127563638	No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCAACTGCTCT	987	0.12799547671506806	No Hit
CCGCTAGGGAGTCTAATTCTACTAAAGGCTTAACCATTCTTCTCTTCACTTT	974	0.1263096193723164	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCT	956	0.12397535535927566	No Hit
CCGCTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	932	0.12086300334188799	No Hit
CCGCTAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	927	0.1202145966715989	No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	925	0.11995523400348324	No Hit
CCGCTAGGGAGGGACAGAGAGAGAGATGGGGACCGGGAGGATCGATTCAGAA	920	0.11930682733319413	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	918	0.1190474646650785	No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCGGCGGTTAAGAGCACTGACTGCTC	903	0.11710224465421122	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	893	0.11580543131363302	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	892	0.1156757499795752	No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	891	0.11554606864551736	No Hit
CCGCTAGGGGAAAATGGAGAAATATTTTCAATGAACTCTCATTTCCAAAAGC	886	0.11489766197522829	No Hit
CCGCTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	840	0.10893232060856857	No Hit
CCGCTAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	835	0.10828391393827948	No Hit
CCGCTAGGGGCAGCGAGTATCTGCCCTGCGTGCTGCCACCTGCTGTGTACGT	826	0.10711678193175908	No Hit
CCGCTAGGGGAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGCAGCCAA	825	0.10698710059770128	No Hit
CCGCTAGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTC	820	0.10633869392741217	No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	810	0.10504188058683397	No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	793	0.10283729790785104	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.020489650781135516	0.0	0.0	0.0	0.0
7	0.020878694783308976	0.0	0.0	0.0	0.0
8	0.021138057451424615	0.0	0.0	0.0	0.0
9	0.021138057451424615	0.0	0.0	0.0	0.0
10	0.027233080152142142	0.0	0.0	0.0	0.0
11	0.05161317095501225	0.0	0.0	0.0	0.0
12	0.06030182033688617	0.0	0.0	0.0	0.0
13	0.06548907369919896	0.0	0.0	0.0	0.0
14	0.07339963507672596	0.0	0.0	0.0	0.0
15	0.10089207789698375	0.0	0.0	0.0	0.0
16	0.11126658462160932	0.0	0.0	0.0	0.0
17	0.11528670597740173	0.0	0.0	0.0	0.0
18	0.12138172867811926	0.0	0.0	0.0	0.0
19	0.1340904994157856	0.0	0.0	0.0	0.0
20	0.14381659947012207	0.0	0.0	0.0	0.0
21	0.15004130350489742	0.0	0.0	0.0	0.0
22	0.1579518648824244	0.0	0.0	0.0	0.0
23	0.1631391182447372	0.0	0.0	0.0	0.0
24	0.17079031695414856	0.0	0.0	0.0	0.0
25	0.17636661431863482	0.0	0.0	0.0	0.0
26	0.18259131835341016	0.0	0.0	0.0	0.0
27	0.1901128357287637	0.0	0.0	0.0	0.0
28	0.20009829845121582	0.0	0.0	0.0	0.0
29	0.20995407983961012	0.0	0.0	0.0	0.0
30	0.2183833665533684	0.0	0.0	0.0	0.0
31	0.22746105993741578	0.0	0.0	0.0	0.0
32	0.239521424004793	0.0	0.0	0.0	0.0
33	0.24756166671637783	0.0	0.0	0.0	0.0
34	0.2556019094279627	0.0	0.0	0.0	0.0
35	0.26999653750838065	0.0	0.0	0.0	0.0
36	0.2875035176061863	0.0	0.0	0.0	0.0
37	0.3016387830184887	0.0	0.0	0.0	0.0
38	0.31214297107717204	0.0	0.0	0.0	0.0
39	0.32057225779093035	0.0	0.0	0.0	0.0
40	0.3283531378343995	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGTA	35	1.0189797E-7	46.000004	34
CGGTATA	35	1.0189797E-7	46.000004	36
AACGTCG	30	1.8606006E-6	46.0	32
ACCGGAT	25	3.4161025E-5	46.0	42
CGAACAA	20	6.310156E-4	46.0	35
CGTATCG	20	6.310156E-4	46.0	13
CGACGTT	20	6.310156E-4	46.0	26
CGACGTA	20	6.310156E-4	46.0	14
GCGAATA	25	3.4161025E-5	46.0	11
TCCGACG	40	5.6079443E-9	46.0	27
TTAGATA	25	3.4161025E-5	46.0	46
TAGCGAC	85	0.0	46.0	30
ATTACCG	20	6.310156E-4	46.0	29
TCGGGTA	60	0.0	46.0	42
TTACTAC	20	6.310156E-4	46.0	19
AAGGTCG	20	6.310156E-4	46.0	32
TAACGTT	25	3.4161025E-5	46.0	39
AAGCGAT	25	3.4161025E-5	46.0	22
TAACGCC	20	6.310156E-4	46.0	22
TAACGAG	20	6.310156E-4	46.0	31
>>END_MODULE