FastQCFastQC Report
Fri 17 Jun 2016
SRR1527486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035450
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAA52990.5117581727751219No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC45090.43546284224250326No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAA40560.391713747645951No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAA31070.3000627746390458No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGA28940.27949200830556764No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20030.1934424646289053No Hit
TACTTCGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAA17430.16833260901057512No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG16860.16282775604809502No Hit
TACTTCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC16440.15877154860205708No Hit
TACTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG15530.1499830991356415No Hit
TACTTCGGGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA15260.14737553720604568No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA14960.14447824617316143No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14400.13906996957844417No Hit
TACTTCGGGAGGAGGCTTAAGTACTTTTACATACAAATTTACCCTAGCCA14400.13906996957844417No Hit
TACTTCGGGAAGACGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAA14340.1384905113718673No Hit
TACTTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC13930.13453088029359217No Hit
TACTTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA13800.13327538751267565No Hit
TACTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA13220.12767395818243277Illumina Single End Adapter 2 (97% over 34bp)
TACTTCGGGATTCATGAATTTTGTTTTAATTGTGAGCAAATCTGTTGATT13150.1269979236080931No Hit
TACTTCGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAA12790.123521174368632No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG12300.11878893234825438No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT12140.11724371046404945No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAA11730.1132840793857743No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAGAAGGAGAAGAAGAA11260.10874499010092231No Hit
TACTTCGGGAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAA10400.10043942247332077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.85612E-444.040
AACGTCG408.310963E-944.027
ATAGGCG254.442533E-544.013
CGACGTT254.442533E-544.044
CGACGTA351.4458965E-744.037
CGACGAA207.85612E-444.043
TAGACGT351.4458965E-744.011
ACGCACT351.4458965E-744.019
ATGGATA207.85612E-444.024
GACGTTA254.442533E-544.09
TTAGACG351.4458965E-744.037
TATGGAC502.7284841E-1144.044
ACCGAAT254.442533E-544.035
GTTAGCG302.527473E-644.040
CGCGATA302.527473E-644.015
CACGACC207.85612E-444.012
TACGGGT750.044.028
CGTTCAC302.527473E-644.042
CTTGGTA254.442533E-544.024
ATACTAC207.85612E-444.014
Produced by FastQC (version 0.11.5)