##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1035450
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43849437442658	31.0	31.0	33.0	30.0	34.0
2	30.659936259597277	31.0	30.0	33.0	27.0	34.0
3	31.950592496016224	33.0	31.0	34.0	30.0	34.0
4	35.971805495195326	37.0	35.0	37.0	35.0	37.0
5	36.2311980298421	37.0	35.0	37.0	35.0	37.0
6	36.22625718286735	37.0	37.0	37.0	35.0	37.0
7	36.40007050074847	37.0	37.0	37.0	35.0	37.0
8	36.54352407165967	37.0	37.0	37.0	35.0	37.0
9	38.26992322178763	39.0	39.0	39.0	37.0	39.0
10	36.336922111159396	39.0	35.0	39.0	31.0	39.0
11	36.73085421797286	38.0	37.0	39.0	32.0	39.0
12	36.90054951953257	39.0	37.0	39.0	34.0	39.0
13	36.92178569703994	39.0	37.0	39.0	33.0	39.0
14	37.80079192621565	40.0	38.0	40.0	33.0	40.0
15	37.84972717176107	40.0	38.0	40.0	33.0	40.0
16	37.948300738809216	40.0	38.0	40.0	34.0	40.0
17	37.910853252209186	40.0	38.0	40.0	34.0	40.0
18	37.7242995798928	40.0	37.0	40.0	33.0	40.0
19	37.64065961659182	39.0	37.0	40.0	33.0	40.0
20	37.49984934086629	39.0	37.0	40.0	33.0	40.0
21	37.39397942923367	39.0	37.0	40.0	33.0	40.0
22	37.238948283355064	39.0	36.0	40.0	33.0	40.0
23	36.97744072625429	38.0	36.0	40.0	32.0	40.0
24	36.646805736636246	38.0	35.0	40.0	32.0	40.0
25	36.28627263508619	38.0	35.0	40.0	31.0	40.0
26	35.603259452412	38.0	35.0	39.0	30.0	40.0
27	35.50154715341156	37.0	34.0	39.0	30.0	40.0
28	35.27228934279782	37.0	34.0	39.0	30.0	40.0
29	34.87040030904438	36.0	34.0	38.0	29.0	40.0
30	34.510895745811	36.0	33.0	38.0	28.0	40.0
31	34.379266019605005	36.0	33.0	38.0	27.0	40.0
32	34.07865275966971	36.0	33.0	38.0	26.0	40.0
33	33.66375488917862	35.0	33.0	38.0	25.0	39.0
34	33.210261239074796	35.0	32.0	38.0	24.0	39.0
35	32.95569462552513	35.0	31.0	38.0	23.0	39.0
36	32.38569124535226	35.0	31.0	38.0	21.0	39.0
37	31.828345163938383	34.0	30.0	38.0	19.0	39.0
38	31.27723598435463	34.0	29.0	37.0	17.0	39.0
39	30.3493070645613	33.0	28.0	37.0	14.0	38.0
40	29.87298758993674	33.0	27.0	36.0	12.0	38.0
41	29.473779516152398	33.0	27.0	36.0	12.0	38.0
42	29.148210922787193	32.0	26.0	35.0	10.0	38.0
43	28.704982374812882	32.0	26.0	35.0	10.0	38.0
44	28.28637983485441	31.0	24.0	35.0	10.0	37.0
45	27.91607127335941	31.0	24.0	34.0	10.0	37.0
46	27.739424404848133	31.0	24.0	34.0	10.0	37.0
47	27.312818581293158	30.0	23.0	34.0	10.0	36.0
48	26.694422714761696	30.0	23.0	33.0	9.0	35.0
49	25.99730165628471	29.0	21.0	33.0	9.0	35.0
50	28.197017721763483	32.0	25.0	34.0	9.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	8.0
12	8.0
13	11.0
14	27.0
15	59.0
16	148.0
17	346.0
18	629.0
19	1235.0
20	2216.0
21	3405.0
22	5076.0
23	7358.0
24	10444.0
25	14504.0
26	19602.0
27	26529.0
28	33372.0
29	38334.0
30	39424.0
31	41881.0
32	53205.0
33	76816.0
34	117488.0
35	216988.0
36	246065.0
37	78597.0
38	1670.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.9436476894104	0.7588005215123859	0.15635713940798687	0.14119464966922596
8	98.18996571538945	1.3633685837075666	0.23062436621758658	0.21604133468540251
9	92.22009754213144	4.656236418948283	1.4146506349896182	1.7090154039306582
10	41.67328214785842	39.89927084842339	7.198802453039741	11.22864455067845
11	27.754406296779177	33.65889226906177	22.276787870008206	16.30991356415085
12	31.10039113428944	27.215027282823893	24.093389347626637	17.59119223526003
13	23.36829397846347	27.93703220821865	26.4196243179294	22.27504949538848
14	23.98551354483558	31.501376213240622	23.224105461393595	21.289004780530206
15	24.211405669032786	30.284900284900285	23.706600994736586	21.79709305133034
16	26.15481191752378	30.762470423487372	25.031145878603507	18.051571780385338
17	28.96083828287218	29.45019073832633	21.074798396832296	20.51417258196919
18	26.80061808875368	30.797237915881986	21.858129315756432	20.5440146796079
19	26.28345163938384	30.549422956202616	22.17663817663818	20.990487227775365
20	23.599884108358683	31.436959775942825	24.18745472982761	20.775701385870875
21	24.531459751798735	29.019460138104208	26.849582307209424	19.599497802887633
22	22.79067072287411	31.710270896711574	22.261528803901683	23.237529576512628
23	23.521464097735283	32.84871311989956	22.805253754406294	20.82456902795886
24	23.021488241827225	30.578878748370276	24.65642957168381	21.74320343811869
25	23.71761070066155	29.004104495629917	24.03747163069197	23.240813173016566
26	24.097831860543725	29.104350765367716	22.3404316963639	24.45738567772466
27	24.379834854411126	28.957458110000484	22.540827659471727	24.121879376116663
28	26.28779757593317	30.57771983195712	21.767637259162683	21.366845332947026
29	26.86136462407649	31.647109952194697	20.790091264667534	20.70143415906128
30	26.00849872036313	32.84272538509826	20.547588005215122	20.601187889323484
31	26.529335071707955	32.845236370660096	21.180646095900332	19.444782461731613
32	25.631078275146074	31.97498672074943	22.851224105461394	19.5427108986431
33	26.981602201941186	32.449273262832584	21.321357863730743	19.247766671495484
34	24.648413733159497	34.76836158192091	21.157757496740548	19.425467188179052
35	24.711091795837557	36.635762228982564	20.03476749239461	18.61837848278526
36	27.13718673040707	35.40199913081269	19.480129412332804	17.98068472644744
37	25.5260997633879	36.4565164904148	20.14882418272249	17.868559563474818
38	27.41223622579555	34.40050219711237	18.569027958858467	19.618233618233617
39	26.742479115360474	33.19638804384567	18.68462987107055	21.37650296972331
40	25.835144140228888	35.05046115215607	20.602250229368874	18.512144478246174
41	23.662948476507797	35.33825872808924	20.750784682988073	20.24800811241489
42	23.681780868221548	35.754889178617994	20.382538992708486	20.18079096045198
43	22.760152590661065	35.3492684340142	20.0625814863103	21.82799748901444
44	23.732869766768072	34.635569076247045	20.453426046646385	21.1781351103385
45	24.110000482881837	35.11825776232556	21.447679752764497	19.324062002028104
46	23.606258148631028	35.364913805591776	19.41609928050606	21.61272876527114
47	23.94427543580086	36.01052682408615	19.99826162538027	20.046936114732723
48	23.892607079047757	36.45777198319571	19.606451301366555	20.043169636389976
49	23.015403930658167	35.96204548746922	20.481529769665364	20.541020812207254
50	20.94557921676566	36.17016755999807	21.632913226133567	21.251339997102708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	23.5
4	47.0
5	97.5
6	148.0
7	240.5
8	333.0
9	1625.0
10	2917.0
11	2758.5
12	2600.0
13	2398.5
14	2197.0
15	2509.0
16	2821.0
17	3463.5
18	4106.0
19	5171.5
20	6237.0
21	7750.5
22	9264.0
23	11904.5
24	14545.0
25	17589.5
26	20634.0
27	23990.5
28	27347.0
29	30185.0
30	33023.0
31	36717.0
32	40411.0
33	40637.5
34	40864.0
35	40259.0
36	39654.0
37	41294.5
38	42935.0
39	43839.0
40	44743.0
41	44391.0
42	44039.0
43	44743.5
44	45448.0
45	48198.0
46	50948.0
47	50489.0
48	50030.0
49	52020.0
50	54010.0
51	57167.5
52	60325.0
53	62567.5
54	64810.0
55	66560.5
56	68311.0
57	64488.5
58	60666.0
59	57014.0
60	53362.0
61	50155.5
62	46949.0
63	41697.0
64	36445.0
65	29017.0
66	21589.0
67	18436.0
68	15283.0
69	13226.0
70	11169.0
71	9037.5
72	6906.0
73	6410.5
74	5915.0
75	4177.5
76	2440.0
77	1650.0
78	860.0
79	826.0
80	792.0
81	556.0
82	320.0
83	163.5
84	7.0
85	3.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1035450.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.601424287024784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94592793583922	18.86837765233649
2	7.6463775581408715	3.609308020169345
3	2.6820801095225435	1.8990273190989437
4	1.400103573455742	1.3217775393163418
5	0.8956622800948629	1.0569452745201446
6	0.6476783908428492	0.9171679502291731
7	0.5141576279623149	0.8494396629564184
8	0.45610993150294565	0.8611875211941465
9	0.3661964797470858	0.7778482641833265
>10	3.9731219089776193	21.836475272497637
>50	0.9226925470570909	14.99956796529847
>100	0.5163768154682357	24.42552970789052
>500	0.021101937170576936	3.3608625773189993
>1k	0.011999140744053552	4.699016340016647
>5k	4.1376347393288116E-4	0.5174689329734244
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAA	5299	0.5117581727751219	No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4509	0.43546284224250326	No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAA	4056	0.391713747645951	No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAA	3107	0.3000627746390458	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGA	2894	0.27949200830556764	No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2003	0.1934424646289053	No Hit
TACTTCGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAA	1743	0.16833260901057512	No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1686	0.16282775604809502	No Hit
TACTTCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	1644	0.15877154860205708	No Hit
TACTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1553	0.1499830991356415	No Hit
TACTTCGGGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA	1526	0.14737553720604568	No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	1496	0.14447824617316143	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1440	0.13906996957844417	No Hit
TACTTCGGGAGGAGGCTTAAGTACTTTTACATACAAATTTACCCTAGCCA	1440	0.13906996957844417	No Hit
TACTTCGGGAAGACGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAA	1434	0.1384905113718673	No Hit
TACTTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1393	0.13453088029359217	No Hit
TACTTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	1380	0.13327538751267565	No Hit
TACTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1322	0.12767395818243277	Illumina Single End Adapter 2 (97% over 34bp)
TACTTCGGGATTCATGAATTTTGTTTTAATTGTGAGCAAATCTGTTGATT	1315	0.1269979236080931	No Hit
TACTTCGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAA	1279	0.123521174368632	No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1230	0.11878893234825438	No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1214	0.11724371046404945	No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAA	1173	0.1132840793857743	No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAGAAGGAGAAGAAGAA	1126	0.10874499010092231	No Hit
TACTTCGGGAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAA	1040	0.10043942247332077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.726109421024675E-4	0.0	0.0	0.0	0.0
8	0.006663769375633783	0.0	0.0	0.0	0.0
9	0.016611135255203052	0.0	0.0	0.0	0.0
10	0.12603215993046502	0.0	0.0	0.0	0.0
11	0.25602395093920516	0.0	0.0	0.0	0.0
12	0.3531797769085905	0.0	0.0	0.0	0.0
13	0.42532232362740835	0.0	0.0	0.0	0.0
14	0.5069293543869815	0.0	0.0	0.0	0.0
15	0.6038920276208412	0.0	0.0	0.0	0.0
16	0.6915833695494712	0.0	0.0	0.0	0.0
17	0.7575450287314694	0.0	0.0	0.0	0.0
18	0.8239895697522817	0.0	0.0	0.0	0.0
19	0.896421845574388	0.0	0.0	0.0	0.0
20	0.9771596890240958	0.0	0.0	0.0	0.0
21	1.057221497899464	0.0	0.0	0.0	0.0
22	1.148969047274132	0.0	0.0	0.0	0.0
23	1.2490221642764017	0.0	0.0	0.0	0.0
24	1.3583466125839008	0.0	0.0	0.0	0.0
25	1.4684436718335023	0.0	0.0	0.0	0.0
26	1.5834661258390073	0.0	0.0	0.0	0.0
27	1.7171278188227341	0.0	0.0	0.0	0.0
28	1.863441015983389	0.0	0.0	0.0	0.0
29	1.9567337872422619	0.0	0.0	0.0	0.0
30	2.052827273166256	0.0	0.0	0.0	0.0
31	2.129605485537689	0.0	0.0	0.0	0.0
32	2.201554879520981	0.0	0.0	0.0	0.0
33	2.268675455116133	0.0	0.0	0.0	0.0
34	2.350379062243469	0.0	0.0	0.0	0.0
35	2.458448017770052	0.0	0.0	0.0	0.0
36	2.5380269448066057	0.0	0.0	0.0	0.0
37	2.608527693273456	0.0	0.0	0.0	0.0
38	2.6831812255541068	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.85612E-4	44.0	40
AACGTCG	40	8.310963E-9	44.0	27
ATAGGCG	25	4.442533E-5	44.0	13
CGACGTT	25	4.442533E-5	44.0	44
CGACGTA	35	1.4458965E-7	44.0	37
CGACGAA	20	7.85612E-4	44.0	43
TAGACGT	35	1.4458965E-7	44.0	11
ACGCACT	35	1.4458965E-7	44.0	19
ATGGATA	20	7.85612E-4	44.0	24
GACGTTA	25	4.442533E-5	44.0	9
TTAGACG	35	1.4458965E-7	44.0	37
TATGGAC	50	2.7284841E-11	44.0	44
ACCGAAT	25	4.442533E-5	44.0	35
GTTAGCG	30	2.527473E-6	44.0	40
CGCGATA	30	2.527473E-6	44.0	15
CACGACC	20	7.85612E-4	44.0	12
TACGGGT	75	0.0	44.0	28
CGTTCAC	30	2.527473E-6	44.0	42
CTTGGTA	25	4.442533E-5	44.0	24
ATACTAC	20	7.85612E-4	44.0	14
>>END_MODULE