FastQCFastQC Report
Fri 17 Jun 2016
SRR1527485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816750
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAA40040.4902356902356902No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA39640.4853382307927762No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAA32370.39632690541781446No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAA23940.2931129476584022No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG21360.261524334251607No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16370.20042852770125497No Hit
TACTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14840.18169574533210897Illumina Single End Adapter 2 (97% over 34bp)
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT14130.17300275482093663No Hit
TACTTCGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAA13840.169452096724824No Hit
TACTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG12750.1561065197428834No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG12690.15537190082644628No Hit
TACTTCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG11860.14520967248239977No Hit
TACTTCGGGAAGACGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAA11320.1385981022344659No Hit
TACTTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA11310.138475665748393No Hit
TACTTCGGGAGGAGGCTTAAGTACTTTTACATACAAATTTACCCTAGCCAGC11260.13786348331802878No Hit
TACTTCGGGATTCATGAATTTTGTTTTAATTGTGAGCAAATCTGTTGATTTC10720.13125191307009487No Hit
TACTTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA10230.12525252525252525No Hit
TACTTCGGGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA10100.1236608509335782No Hit
TACTTCGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA9980.122191613100704No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAA9620.11778389960208142No Hit
TACTTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9300.11386593204775024Illumina Single End Adapter 2 (97% over 34bp)
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9270.11349862258953167No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT9010.1103152739516376No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8940.10945821854912764No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGGAAAAAAAAAAAAAAAAAAAAAA8800.10774410774410774No Hit
TACTTCGGGAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAA8720.10676461585552495No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC8690.10639730639730641No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAGAAGGAGAAGAAGAAGC8330.1019895928986838No Hit
TACTTCGGTATTTGACTCTATTTGTATCCTAGCAGCATGTTTCCTGTTTCGT8170.10003060912151822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA700.046.00000429
TCGATTG351.0190888E-746.00000418
TCGATAG351.0190888E-746.00000427
CACTCGA351.0190888E-746.00000424
ACTCGAT351.0190888E-746.00000425
CGGTACC351.0190888E-746.0000046
CGTCATT351.0190888E-746.00000413
CGATAGC351.0190888E-746.00000428
GCATAAC253.416282E-546.036
AACGTTG253.416282E-546.045
CTAGATT206.3103775E-446.034
ACTATCA301.860737E-646.041
CGAACCC253.416282E-546.022
ACACGAT206.3103775E-446.026
CGTATTG301.860737E-646.040
GTATTAC206.3103775E-446.035
CGACGTA301.860737E-646.037
CGAAACT253.416282E-546.042
CGAAACC206.3103775E-446.028
ACGTTGT301.860737E-646.024
Produced by FastQC (version 0.11.5)