##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527485_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	816750
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.969487603305787	33.0	31.0	34.0	30.0	34.0
2	31.641541475359656	33.0	31.0	34.0	30.0	34.0
3	32.516683195592286	34.0	31.0	34.0	31.0	34.0
4	36.237103152739515	37.0	37.0	37.0	35.0	37.0
5	36.44615243342516	37.0	37.0	37.0	35.0	37.0
6	36.37692561983471	37.0	37.0	37.0	35.0	37.0
7	36.50462932353842	37.0	37.0	37.0	35.0	37.0
8	36.620210590756045	37.0	37.0	37.0	35.0	37.0
9	38.42014937251301	39.0	39.0	39.0	37.0	39.0
10	36.88892072237527	39.0	37.0	39.0	32.0	39.0
11	37.25785736149373	39.0	37.0	39.0	34.0	39.0
12	37.46219651056015	39.0	37.0	39.0	35.0	39.0
13	37.44314661769207	39.0	37.0	39.0	35.0	39.0
14	38.26273890419345	40.0	38.0	40.0	35.0	40.0
15	38.39289868380777	40.0	38.0	40.0	35.0	40.0
16	38.48824242424242	40.0	38.0	40.0	35.0	40.0
17	38.50866850321396	40.0	38.0	40.0	35.0	40.0
18	38.3750866238139	40.0	38.0	40.0	35.0	40.0
19	38.30063666972758	40.0	38.0	40.0	35.0	40.0
20	38.294734006734004	40.0	38.0	40.0	35.0	40.0
21	38.248037955310686	40.0	38.0	40.0	34.0	40.0
22	38.215029078665445	40.0	38.0	40.0	35.0	40.0
23	37.99055157636976	40.0	38.0	40.0	34.0	40.0
24	37.789635751453936	40.0	37.0	40.0	34.0	40.0
25	37.591367003367004	40.0	36.0	40.0	34.0	40.0
26	37.44607897153352	40.0	35.0	40.0	34.0	40.0
27	37.408461585552494	40.0	35.0	40.0	33.0	40.0
28	37.29598041016223	40.0	35.0	40.0	33.0	40.0
29	37.181519436792165	40.0	35.0	40.0	33.0	40.0
30	36.96783593510866	39.0	35.0	40.0	33.0	40.0
31	36.817973676155496	39.0	35.0	40.0	33.0	40.0
32	36.57183348637894	39.0	35.0	40.0	32.0	40.0
33	36.272999081726354	39.0	35.0	40.0	31.0	40.0
34	35.87205632078359	39.0	35.0	40.0	29.0	40.0
35	35.62529415365779	39.0	35.0	40.0	27.0	40.0
36	35.29306029996939	39.0	35.0	40.0	25.0	40.0
37	34.833987144168965	38.0	35.0	40.0	22.0	40.0
38	34.66588429752066	39.0	35.0	40.0	21.0	40.0
39	34.32906519742883	38.0	35.0	40.0	18.0	40.0
40	33.94887786960514	38.0	34.0	40.0	15.0	40.0
41	33.67904499540863	38.0	34.0	40.0	15.0	40.0
42	33.43584695439241	37.0	34.0	40.0	10.0	40.0
43	33.294227119681665	37.0	33.0	40.0	10.0	40.0
44	33.16282705846342	37.0	33.0	40.0	10.0	40.0
45	32.98538353229262	36.0	33.0	40.0	10.0	40.0
46	32.7696149372513	36.0	33.0	40.0	10.0	40.0
47	32.563182124273034	35.0	33.0	40.0	10.0	40.0
48	32.33906825834099	35.0	33.0	40.0	10.0	40.0
49	32.11526538108356	35.0	32.0	39.0	9.0	40.0
50	32.11841444750536	35.0	33.0	39.0	9.0	40.0
51	32.04869666360575	35.0	33.0	39.0	9.0	40.0
52	31.679822467095196	35.0	32.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	12.0
13	10.0
14	18.0
15	26.0
16	62.0
17	190.0
18	362.0
19	770.0
20	1381.0
21	2075.0
22	3372.0
23	5281.0
24	7785.0
25	10920.0
26	15730.0
27	20284.0
28	21769.0
29	18249.0
30	14393.0
31	14221.0
32	18688.0
33	23221.0
34	37968.0
35	48532.0
36	80133.0
37	165795.0
38	250612.0
39	54890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.9610039791858	0.7533516988062442	0.1494949494949495	0.13614937251300888
8	98.21707988980715	1.3519436792164063	0.21377410468319558	0.21720232629323538
9	92.20079583715948	4.683318028772574	1.3841444750535659	1.7317416590143864
10	41.874502601775326	39.591796755433116	7.194367921640649	11.339332721150903
11	27.723293541475357	33.47070707070707	22.30719314355678	16.498806244260788
12	30.566146311600857	27.60281603917967	24.169329660238752	17.661707988980716
13	23.332598714416896	28.22332415059688	26.480685644322012	21.96339149066422
14	23.86176920722375	31.559351086623817	23.31202938475666	21.266850321395776
15	23.780593816957456	30.53884297520661	23.71460055096419	21.96596265687175
16	26.097337006427917	31.048056320783594	24.66727884909703	18.18732782369146
17	28.565289256198344	29.843281297826753	21.1089072543618	20.4825221916131
18	26.485705540250994	31.088093051729416	21.774839302112028	20.651362105907562
19	26.32029384756657	30.911906948270584	21.74214876033058	21.025650443832262
20	23.608325681052953	31.34104683195592	24.301805938169572	20.74882154882155
21	24.62136516681971	29.455524946434036	26.350290786654423	19.572819100091827
22	22.894643403734314	31.877441077441077	22.214631160085705	23.013284358738904
23	23.58800122436486	32.744046525864704	22.835016835016837	20.8329354147536
24	23.164493419038873	30.519498010407105	24.610958065503517	21.705050505050504
25	23.678849097030916	29.3425160697888	24.08337924701561	22.89525558616468
26	23.74900520355066	29.50462197734925	22.451790633608816	24.294582185491276
27	24.339761248852156	29.262320171411083	22.560759106213652	23.83715947352311
28	26.25785123966942	30.693602693602696	21.70811141720233	21.34043464952556
29	26.58732782369146	31.992898683807773	20.743434343434345	20.676339149066422
30	26.088399142944596	32.830241812059995	20.422161003979188	20.65919804101622
31	26.41554943373125	33.07891031527395	21.15139271502908	19.35414753596572
32	25.896296296296295	32.32053872053872	22.40391796755433	19.37924701561065
33	26.771717171717174	32.79559228650138	21.24775022956841	19.18494031221304
34	25.005448423630245	34.95549433731252	21.023691460055097	19.015365779002142
35	25.121028466483015	36.430609121518216	19.918457300275485	18.529905111723295
36	27.37875726966636	35.495684113865934	19.262565044383226	17.86299357208448
37	26.231404958677686	36.3504132231405	19.649954086317724	17.768227731864094
38	27.84695439240894	34.495990205081114	18.278787878787877	19.37826752372207
39	27.295745332108968	33.186042240587696	18.542883379247016	20.97532904805632
40	26.61524334251607	34.71111111111111	20.352494643403734	18.321150902969087
41	24.563942454851546	35.15053565962657	20.341720232629324	19.943801652892564
42	24.701316192225285	34.77171717171717	20.535782063054793	19.991184573002755
43	23.755249464340373	34.81787572696663	20.123048668503213	21.303826140189777
44	24.561248852157945	33.990572390572396	20.389715335169882	21.058463422099784
45	25.048546066727884	34.29923477196205	21.311906948270583	19.340312213039486
46	24.230180593816957	34.77918579736762	19.48992959902051	21.50070400979492
47	24.352249770431587	35.20636669727579	20.018120599938783	20.423262932353843
48	24.480440771349862	35.529599020508115	19.760514233241505	20.22944597490052
49	23.71043771043771	34.829139883685336	20.825589225589226	20.634833180287725
50	21.000795837159473	35.83985307621671	21.574043464952556	21.585307621671255
51	21.989960208142026	36.403183348637896	21.15408631772268	20.4527701254974
52	22.87505356596266	34.25858585858586	21.76039179675543	21.105968778696052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	15.0
4	30.0
5	77.0
6	124.0
7	221.5
8	319.0
9	1342.0
10	2365.0
11	2147.0
12	1929.0
13	1739.0
14	1652.0
15	1755.0
16	2090.5
17	2426.0
18	2795.0
19	3164.0
20	3641.5
21	4119.0
22	5092.0
23	6065.0
24	7726.5
25	9388.0
26	12429.0
27	15470.0
28	19659.5
29	23849.0
30	28749.0
31	33649.0
32	33247.0
33	32845.0
34	34264.0
35	35683.0
36	35351.0
37	35019.0
38	35424.5
39	35715.0
40	35600.0
41	35775.0
42	35950.0
43	35299.5
44	34649.0
45	35207.0
46	35765.0
47	35531.0
48	35297.0
49	36256.0
50	37215.0
51	39865.0
52	42515.0
53	44575.0
54	46635.0
55	50444.5
56	54254.0
57	49538.0
58	44822.0
59	42555.5
60	40289.0
61	38681.0
62	37073.0
63	33574.0
64	25403.5
65	20732.0
66	16575.5
67	12419.0
68	10989.0
69	9559.0
70	8666.0
71	7773.0
72	6574.5
73	5376.0
74	4305.0
75	3234.0
76	2198.5
77	1163.0
78	713.0
79	263.0
80	237.5
81	212.0
82	250.0
83	288.0
84	150.5
85	13.0
86	7.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	816750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.910746679463223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69086375399704	17.799556987765172
2	8.051225161378172	3.6891916026331137
3	2.9221483857749915	2.0084580427887957
4	1.6308891991844876	1.4945955721915374
5	1.0519556009969786	1.2050544146242133
6	0.8079656835542076	1.1106658260965874
7	0.6584847119284315	1.0560463499104127
8	0.5277243073341774	0.9672446337542826
9	0.4244288246551073	0.8751583156621932
>10	4.805506933742943	25.506548535676576
>50	0.8754947533415967	13.708849399958334
>100	0.5226030219946726	23.26064429750979
>500	0.021011874080198178	3.3832385194033545
>1k	0.009697788037014545	3.9347475020256395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAA	4004	0.4902356902356902	No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3964	0.4853382307927762	No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAA	3237	0.39632690541781446	No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAA	2394	0.2931129476584022	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG	2136	0.261524334251607	No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1637	0.20042852770125497	No Hit
TACTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1484	0.18169574533210897	Illumina Single End Adapter 2 (97% over 34bp)
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1413	0.17300275482093663	No Hit
TACTTCGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAA	1384	0.169452096724824	No Hit
TACTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1275	0.1561065197428834	No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1269	0.15537190082644628	No Hit
TACTTCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	1186	0.14520967248239977	No Hit
TACTTCGGGAAGACGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAA	1132	0.1385981022344659	No Hit
TACTTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1131	0.138475665748393	No Hit
TACTTCGGGAGGAGGCTTAAGTACTTTTACATACAAATTTACCCTAGCCAGC	1126	0.13786348331802878	No Hit
TACTTCGGGATTCATGAATTTTGTTTTAATTGTGAGCAAATCTGTTGATTTC	1072	0.13125191307009487	No Hit
TACTTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1023	0.12525252525252525	No Hit
TACTTCGGGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1010	0.1236608509335782	No Hit
TACTTCGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA	998	0.122191613100704	No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAA	962	0.11778389960208142	No Hit
TACTTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	930	0.11386593204775024	Illumina Single End Adapter 2 (97% over 34bp)
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	927	0.11349862258953167	No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	901	0.1103152739516376	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	894	0.10945821854912764	No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGGAAAAAAAAAAAAAAAAAAAAAA	880	0.10774410774410774	No Hit
TACTTCGGGAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAA	872	0.10676461585552495	No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	869	0.10639730639730641	No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAGAAGGAGAAGAAGAAGC	833	0.1019895928986838	No Hit
TACTTCGGTATTTGACTCTATTTGTATCCTAGCAGCATGTTTCCTGTTTCGT	817	0.10003060912151822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0011019283746556473	0.0	0.0	0.0	0.0
8	0.00869299051117233	0.0	0.0	0.0	0.0
9	0.018487909397000304	0.0	0.0	0.0	0.0
10	0.14153657790021426	0.0	0.0	0.0	0.0
11	0.2782981328435874	0.0	0.0	0.0	0.0
12	0.38689929599020506	0.0	0.0	0.0	0.0
13	0.46538108356290175	0.0	0.0	0.0	0.0
14	0.5528007346189164	0.0	0.0	0.0	0.0
15	0.6583409856137129	0.0	0.0	0.0	0.0
16	0.7631466176920723	0.0	0.0	0.0	0.0
17	0.842363024181206	0.0	0.0	0.0	0.0
18	0.9078665442301805	0.0	0.0	0.0	0.0
19	0.9842669115396389	0.0	0.0	0.0	0.0
20	1.0734006734006734	0.0	0.0	0.0	0.0
21	1.149556167737986	0.0	0.0	0.0	0.0
22	1.2417508417508418	0.0	0.0	0.0	0.0
23	1.3275788184879094	0.0	0.0	0.0	0.0
24	1.4148760330578511	0.0	0.0	0.0	0.0
25	1.487603305785124	0.0	0.0	0.0	0.0
26	1.5696357514539332	0.0	0.0	0.0	0.0
27	1.6598714416896234	0.0	0.0	0.0	0.0
28	1.7779002142638507	0.0	0.0	0.0	0.0
29	1.8385062748699113	0.0	0.0	0.0	0.0
30	1.898989898989899	0.0	0.0	0.0	0.0
31	1.9417202326293235	0.0	0.0	0.0	0.0
32	1.9859198041016224	0.0	0.0	0.0	0.0
33	2.031711049892868	0.0	0.0	0.0	0.0
34	2.090480563207836	0.0	0.0	0.0	0.0
35	2.1780226507499236	0.0	0.0	0.0	0.0
36	2.2376492194674014	0.0	0.0	0.0	0.0
37	2.288215488215488	0.0	0.0	0.0	0.0
38	2.3473523109886747	0.0	0.0	0.0	0.0
39	2.4155494337312517	0.0	0.0	0.0	0.0
40	2.4881542699724517	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTA	70	0.0	46.000004	29
TCGATTG	35	1.0190888E-7	46.000004	18
TCGATAG	35	1.0190888E-7	46.000004	27
CACTCGA	35	1.0190888E-7	46.000004	24
ACTCGAT	35	1.0190888E-7	46.000004	25
CGGTACC	35	1.0190888E-7	46.000004	6
CGTCATT	35	1.0190888E-7	46.000004	13
CGATAGC	35	1.0190888E-7	46.000004	28
GCATAAC	25	3.416282E-5	46.0	36
AACGTTG	25	3.416282E-5	46.0	45
CTAGATT	20	6.3103775E-4	46.0	34
ACTATCA	30	1.860737E-6	46.0	41
CGAACCC	25	3.416282E-5	46.0	22
ACACGAT	20	6.3103775E-4	46.0	26
CGTATTG	30	1.860737E-6	46.0	40
GTATTAC	20	6.3103775E-4	46.0	35
CGACGTA	30	1.860737E-6	46.0	37
CGAAACT	25	3.416282E-5	46.0	42
CGAAACC	20	6.3103775E-4	46.0	28
ACGTTGT	30	1.860737E-6	46.0	24
>>END_MODULE