FastQCFastQC Report
Fri 17 Jun 2016
SRR1527484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143048
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA53510.4681343215682981No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAA52870.4625352566121458No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAA43120.3772370014207627No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG36300.31757196548176453No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAA32490.2842400319146703No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22170.19395510949671405No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT19380.16954668570348752No Hit
TACTTCGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAA19330.16910925875378813No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG18150.15878598274088226No Hit
TACTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG18020.15764867267166383No Hit
TACTTCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG16950.1482877359480967No Hit
TACTTCGGGAGGAGGCTTAAGTACTTTTACATACAAATTTACCCTAGCCAGC16880.14767533821851753No Hit
TACTTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA15990.13988913851386817No Hit
TACTTCGGGAAGACGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAA15730.13761451837543132No Hit
TACTTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA14890.13026574562048138No Hit
TACTTCGGGATTCATGAATTTTGTTTTAATTGTGAGCAAATCTGTTGATTTC14850.12991580406072187No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG14100.12335439981523086No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAA13450.11766784946913866No Hit
TACTTCGGGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA13070.11434340465142322No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC12870.1125936968526256No Hit
TACTTCGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA12190.10664469033671378No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAGAAGGAGAAGAAGAAGC12080.10568235104737508No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT12040.10533240948761557No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC11840.10358270168881797No Hit
TACTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA11580.1013080815503811Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC206.3114386E-446.00000446
AACGTTA206.3114386E-446.00000410
CACGGCG206.3114386E-446.00000438
ACGTTAA206.3114386E-446.00000411
CGCGATA206.3114386E-446.00000415
CGATTGA206.3114386E-446.00000422
TAAAACG206.3114386E-446.00000425
ATACTCG206.3114386E-446.00000444
TTATACG206.3114386E-446.00000434
ACGATTA206.3114386E-446.00000430
GATATAC206.3114386E-446.0000049
TACTCGC206.3114386E-446.00000445
CTCGAGT206.3114386E-446.00000435
TTACGCC206.3114386E-446.00000419
TATACGA206.3114386E-446.00000435
TCGACGG206.3114386E-446.00000417
CAATACG800.046.00000425
AGTACGA206.3114386E-446.00000437
ACTATTG301.8613937E-646.022
CAAAACG253.4171448E-546.042
Produced by FastQC (version 0.11.5)