##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527484_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143048
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.014078148949125	31.0	31.0	33.0	28.0	34.0
2	30.276278861430143	31.0	30.0	33.0	26.0	34.0
3	31.61927670579013	31.0	31.0	34.0	30.0	34.0
4	35.71797597301251	37.0	35.0	37.0	35.0	37.0
5	36.02186784806937	37.0	35.0	37.0	35.0	37.0
6	36.0004374269497	37.0	35.0	37.0	35.0	37.0
7	36.27921662082432	37.0	35.0	37.0	35.0	37.0
8	36.43347610948972	37.0	37.0	37.0	35.0	37.0
9	38.28295049726696	39.0	39.0	39.0	37.0	39.0
10	36.080793632463376	39.0	35.0	39.0	31.0	39.0
11	36.446629537867175	38.0	35.0	39.0	31.0	39.0
12	36.69960666568683	39.0	35.0	39.0	33.0	39.0
13	36.661633632183424	39.0	37.0	39.0	32.0	39.0
14	37.48786490156144	40.0	37.0	40.0	33.0	40.0
15	37.60845738761627	40.0	37.0	40.0	33.0	40.0
16	37.667164458535424	40.0	37.0	40.0	33.0	40.0
17	37.76755131892974	40.0	37.0	40.0	33.0	40.0
18	37.63791896753242	40.0	37.0	40.0	33.0	40.0
19	37.5059621293244	40.0	37.0	40.0	33.0	40.0
20	37.47638419383963	39.0	37.0	40.0	32.0	40.0
21	37.50983773209874	40.0	37.0	40.0	33.0	40.0
22	37.48566814342005	39.0	37.0	40.0	33.0	40.0
23	37.324646034112305	39.0	36.0	40.0	33.0	40.0
24	37.16159951288135	39.0	36.0	40.0	33.0	40.0
25	36.973787627466216	39.0	35.0	40.0	32.0	40.0
26	36.78769657967119	39.0	35.0	40.0	32.0	40.0
27	36.709290423499276	39.0	35.0	40.0	31.0	40.0
28	36.50992784205038	39.0	35.0	40.0	31.0	40.0
29	36.4201022179296	38.0	35.0	40.0	31.0	40.0
30	36.207825918071684	38.0	35.0	40.0	30.0	40.0
31	36.00016534738699	38.0	35.0	40.0	30.0	40.0
32	35.81051889334481	38.0	35.0	40.0	30.0	40.0
33	35.64076574212107	38.0	35.0	40.0	29.0	40.0
34	35.28855043707701	38.0	35.0	40.0	27.0	40.0
35	35.04454493599569	38.0	34.0	40.0	25.0	40.0
36	34.762289072724855	38.0	34.0	40.0	24.0	40.0
37	34.33026084643865	38.0	34.0	40.0	21.0	40.0
38	34.01059972984512	38.0	33.0	40.0	20.0	40.0
39	33.653819437154	38.0	33.0	40.0	17.0	40.0
40	33.517994869856736	37.0	33.0	40.0	15.0	40.0
41	33.420600884652266	37.0	33.0	40.0	15.0	40.0
42	33.307637999454094	37.0	33.0	40.0	13.0	40.0
43	33.177386251496	37.0	33.0	40.0	12.0	40.0
44	33.0416133005788	36.0	33.0	40.0	11.0	40.0
45	32.90184576675695	36.0	33.0	40.0	10.0	40.0
46	32.57473614406394	35.0	33.0	40.0	10.0	40.0
47	32.45991244462175	35.0	33.0	40.0	10.0	40.0
48	32.29426848216348	35.0	32.0	39.0	10.0	40.0
49	32.10242701968771	35.0	32.0	39.0	10.0	40.0
50	31.802421245651978	35.0	31.0	39.0	10.0	40.0
51	31.575986310286183	35.0	31.0	39.0	10.0	40.0
52	31.066157326726437	35.0	30.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	9.0
14	12.0
15	34.0
16	140.0
17	400.0
18	924.0
19	1754.0
20	3003.0
21	4364.0
22	6228.0
23	8909.0
24	12166.0
25	16780.0
26	22829.0
27	29052.0
28	31937.0
29	29368.0
30	26001.0
31	28456.0
32	35809.0
33	47102.0
34	69119.0
35	85230.0
36	134062.0
37	224529.0
38	286134.0
39	38688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.9867442137163	0.7585858161686998	0.14233872943218484	0.11233124068280598
8	98.30050881502788	1.3091313750603648	0.2006914845220848	0.18966832538965991
9	92.2502817029556	4.634188590505386	1.3954794549310265	1.7200502516079814
10	41.87663160252238	39.676374045534395	7.056746523330604	11.390247828612623
11	27.69087562377083	33.459837207186396	22.260220043252776	16.589067125789995
12	30.717345203351044	27.18958433941532	24.390314317508977	17.702756139724666
13	23.099817330505807	27.905564770683295	26.624691176573513	22.369926722237388
14	23.786052729194225	31.466395112016293	23.406016195295383	21.341535963494096
15	23.960761052904164	30.173098592535048	23.850441976189977	22.015698378370814
16	25.949478937017517	30.698710815293843	25.115918141670342	18.235892106018294
17	28.81646265073733	29.350298500150473	21.101388568109126	20.731850281003073
18	26.59205912612594	30.718395028030322	21.926113339072376	20.76343250677137
19	26.202924111673347	30.62146121597693	22.012548904332977	21.163065768016743
20	23.69629271911591	31.32309404329477	24.233540498736712	20.747072738852612
21	24.6637061610711	29.038675541184624	26.534581224935437	19.763037072808842
22	22.85529566562384	31.736549996150643	22.152962955186485	23.25519138303903
23	23.574775512489417	32.7988850861906	22.736228049915663	20.890111351404318
24	23.03752773286861	30.583055129793323	24.536939831048215	21.84247730628985
25	23.78316571132621	29.089941979689392	23.8346946060008	23.292197702983604
26	24.00634094106284	29.241904102014963	22.32373443634913	24.428020520573064
27	24.29049348758757	29.109801163205745	22.430816553635545	24.16888879557114
28	26.30536950329295	30.57229442683072	21.689727815454816	21.432608254421513
29	26.701415863550785	31.73243818282347	20.803150873804075	20.76299507982167
30	26.099603866154354	32.71787361510628	20.476392942378624	20.70612957636075
31	26.659072934819882	32.716823790427	21.020639553194616	19.6034637215585
32	25.720617156934793	31.86401620929305	22.847072038969493	19.56829459480267
33	27.002803031893674	32.27659730824952	21.270235370693094	19.45036428916371
34	24.728007922676913	34.71525255282368	21.107775001574737	19.448964522924673
35	24.888018700876955	36.51815146870472	19.936433115669683	18.657396714748636
36	27.37006669886129	35.17630055780685	19.458325459648236	17.995307283683626
37	25.87056711529175	36.12131773993743	20.037741197220065	17.97037394755076
38	27.80119470048502	33.929283809603795	18.63298829095541	19.636533198955775
39	27.00341542962325	32.79521069981313	18.724935435782225	21.476438434781393
40	26.106252755789782	34.5347701933777	20.824322338169527	18.534654712662984
41	24.05279568312092	34.9013339772258	20.819598127112773	20.226272212540504
42	24.12400879053198	34.99441843212184	20.622143602018465	20.25942917532772
43	23.289835597455223	34.6295168706826	20.3023845017882	21.778263030073976
44	24.11814726940601	33.85212169567682	20.751621979129485	21.278109055787684
45	24.675516688712985	34.22375963214143	21.6146653508864	19.486058328259183
46	24.068368082530217	34.56267803276853	19.716844786920586	21.65210909778067
47	24.352258172885126	35.20412091180773	20.062674533352933	20.380946381954214
48	24.514631056613545	35.57103463721559	19.74212806461321	20.17220624155766
49	23.597434228483845	34.99398100517214	20.808312511810527	20.600272254533493
50	21.01749007915678	35.95946976854865	21.42735913102512	21.595681021269446
51	21.910803395832897	36.54597182270561	20.86561544222115	20.677609339240348
52	22.955116495545244	34.47073088794171	21.40916216991762	21.164990446595418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	20.0
4	40.0
5	102.0
6	164.0
7	287.5
8	411.0
9	1830.5
10	3250.0
11	2998.5
12	2747.0
13	2585.5
14	2575.5
15	2727.0
16	3227.0
17	3727.0
18	4264.5
19	4802.0
20	5734.5
21	6667.0
22	8275.0
23	9883.0
24	12511.5
25	15140.0
26	19120.0
27	23100.0
28	28157.0
29	33214.0
30	39549.0
31	45884.0
32	44372.0
33	42860.0
34	44765.5
35	46671.0
36	46178.5
37	45686.0
38	46560.5
39	47742.0
40	48049.0
41	48837.5
42	49626.0
43	48781.5
44	47937.0
45	49480.5
46	51024.0
47	50454.5
48	49885.0
49	51924.5
50	53964.0
51	57803.0
52	61642.0
53	64267.0
54	66892.0
55	71468.0
56	76044.0
57	69849.5
58	63655.0
59	60687.5
60	57720.0
61	54586.0
62	51452.0
63	46766.0
64	35692.0
65	29304.0
66	23397.5
67	17491.0
68	15410.5
69	13330.0
70	12335.0
71	11340.0
72	9358.0
73	7376.0
74	5971.0
75	4566.0
76	3089.0
77	1612.0
78	1036.5
79	461.0
80	395.5
81	330.0
82	366.5
83	403.0
84	214.5
85	26.0
86	14.5
87	3.0
88	2.5
89	1.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1143048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.018631769379645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70772093254153	18.577813090920273
2	7.383750783365225	3.399276807183053
3	2.7166530361530343	1.8760090765312172
4	1.3375120477731215	1.231507892591936
5	0.8439450664142414	0.9713230358687036
6	0.5973650700941352	0.8250315948231937
7	0.49917658639087914	0.8043253421019301
8	0.4000603653184879	0.7367073787831817
9	0.31723619031359235	0.6572108743874482
>10	3.640616314770037	19.58906650002706
>50	0.9396650639900248	15.08175307111259
>100	0.5721685237561719	26.11365367896586
>500	0.03195622074170109	4.888423286556175
>1k	0.01141293597917896	4.316328104801853
>5k	7.608623986119307E-4	0.9315702653455491
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5351	0.4681343215682981	No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAA	5287	0.4625352566121458	No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAA	4312	0.3772370014207627	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG	3630	0.31757196548176453	No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAA	3249	0.2842400319146703	No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2217	0.19395510949671405	No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1938	0.16954668570348752	No Hit
TACTTCGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAA	1933	0.16910925875378813	No Hit
TACTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1815	0.15878598274088226	No Hit
TACTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1802	0.15764867267166383	No Hit
TACTTCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	1695	0.1482877359480967	No Hit
TACTTCGGGAGGAGGCTTAAGTACTTTTACATACAAATTTACCCTAGCCAGC	1688	0.14767533821851753	No Hit
TACTTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1599	0.13988913851386817	No Hit
TACTTCGGGAAGACGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAA	1573	0.13761451837543132	No Hit
TACTTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1489	0.13026574562048138	No Hit
TACTTCGGGATTCATGAATTTTGTTTTAATTGTGAGCAAATCTGTTGATTTC	1485	0.12991580406072187	No Hit
TACTTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1410	0.12335439981523086	No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAA	1345	0.11766784946913866	No Hit
TACTTCGGGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1307	0.11434340465142322	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1287	0.1125936968526256	No Hit
TACTTCGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA	1219	0.10664469033671378	No Hit
TACTTCGGGGCAGAGTGCAGTGCTTACTTCGGGAAGAAGGAGAAGAAGAAGC	1208	0.10568235104737508	No Hit
TACTTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1204	0.10533240948761557	No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1184	0.10358270168881797	No Hit
TACTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1158	0.1013080815503811	Illumina Single End Adapter 2 (97% over 34bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0012247954591583206	0.0	0.0	0.0	0.0
8	0.007873685094589203	0.0	0.0	0.0	0.0
9	0.015309943239479007	0.0	0.0	0.0	0.0
10	0.11180632834316669	0.0	0.0	0.0	0.0
11	0.2335859911394797	0.0	0.0	0.0	0.0
12	0.3261455336958728	0.0	0.0	0.0	0.0
13	0.3947340794087387	0.0	0.0	0.0	0.0
14	0.4690966608576368	0.0	0.0	0.0	0.0
15	0.5605188933448114	0.0	0.0	0.0	0.0
16	0.6425801891084189	0.0	0.0	0.0	0.0
17	0.7083692023432087	0.0	0.0	0.0	0.0
18	0.7698714314709444	0.0	0.0	0.0	0.0
19	0.8405596265423674	0.0	0.0	0.0	0.0
20	0.9150971787711453	0.0	0.0	0.0	0.0
21	0.9811486481757546	0.0	0.0	0.0	0.0
22	1.0624225754299033	0.0	0.0	0.0	0.0
23	1.150870304659122	0.0	0.0	0.0	0.0
24	1.238968092328581	0.0	0.0	0.0	0.0
25	1.3194546510732708	0.0	0.0	0.0	0.0
26	1.4021283445664574	0.0	0.0	0.0	0.0
27	1.5018616890979206	0.0	0.0	0.0	0.0
28	1.6148928129002456	0.0	0.0	0.0	0.0
29	1.6805068553551556	0.0	0.0	0.0	0.0
30	1.7476956348289836	0.0	0.0	0.0	0.0
31	1.791875756748623	0.0	0.0	0.0	0.0
32	1.8366682763978415	0.0	0.0	0.0	0.0
33	1.8833854746257375	0.0	0.0	0.0	0.0
34	1.9436629082943149	0.0	0.0	0.0	0.0
35	2.03464771383179	0.0	0.0	0.0	0.0
36	2.0854767253868602	0.0	0.0	0.0	0.0
37	2.1418173165081433	0.0	0.0	0.0	0.0
38	2.1961457436608085	0.0	0.0	0.0	0.0
39	2.2619347568955983	0.0	0.0	0.0	0.0
40	2.3290360509794863	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	6.3114386E-4	46.000004	46
AACGTTA	20	6.3114386E-4	46.000004	10
CACGGCG	20	6.3114386E-4	46.000004	38
ACGTTAA	20	6.3114386E-4	46.000004	11
CGCGATA	20	6.3114386E-4	46.000004	15
CGATTGA	20	6.3114386E-4	46.000004	22
TAAAACG	20	6.3114386E-4	46.000004	25
ATACTCG	20	6.3114386E-4	46.000004	44
TTATACG	20	6.3114386E-4	46.000004	34
ACGATTA	20	6.3114386E-4	46.000004	30
GATATAC	20	6.3114386E-4	46.000004	9
TACTCGC	20	6.3114386E-4	46.000004	45
CTCGAGT	20	6.3114386E-4	46.000004	35
TTACGCC	20	6.3114386E-4	46.000004	19
TATACGA	20	6.3114386E-4	46.000004	35
TCGACGG	20	6.3114386E-4	46.000004	17
CAATACG	80	0.0	46.000004	25
AGTACGA	20	6.3114386E-4	46.000004	37
ACTATTG	30	1.8613937E-6	46.0	22
CAAAACG	25	3.4171448E-5	46.0	42
>>END_MODULE