FastQCFastQC Report
Fri 17 Jun 2016
SRR1527483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670525
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC104700.6267490758893163No Hit
ACAATAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA98920.5921491746606605No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT97470.5834692686430912No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC85680.512892653507131No Hit
ACAATAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG83680.5009203693449664No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC74330.4449499408868469No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG61000.36515466694602No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC57410.34366441687493454No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA53650.3211565226500651No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG52780.3159485790395235No Hit
ACAATAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT52680.3153499648314153No Hit
ACAATAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA49850.2984091827419524No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG47660.28529953158438215No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG43550.26069648763113396No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA40270.241061941605184No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG39820.23836817766869697No Hit
ACAATAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC39250.23495607668248003No Hit
ACAATAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT38390.22980799449274927No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC38180.228550904655722No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC37960.2272339533978839No Hit
ACAATAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG35910.2149623621316652No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC35620.21322638092815135No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG34620.20724023884706902No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC34550.20682120890139327No Hit
ACAATAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA33170.1985603328294997No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC28300.16940782089462894No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG27720.1659358584876012No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC24950.14935424492300325No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT24550.1469597880905703No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC24070.14408643989165085No Hit
ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT24010.14372727136678587No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT23870.14288921147543437No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT23520.14079406174705558No Hit
ACAATAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTT23090.1382200206521902No Hit
ACAATAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA20760.12427230960326842No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA20350.1218179913500247No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGA19460.11649032489786146No Hit
ACAATAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT19100.11433531374867184No Hit
ACAATAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT18710.11200071833704973No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA18070.10816958740515706No Hit
ACAATAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT17700.10595471483515662No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC17380.10403914936921027No Hit
ACAATAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT17060.10212358390326395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT254.4437103E-544.037
GACGTAT207.8575086E-444.09
CGCATGA207.8575086E-444.040
CCGTAGA408.3164196E-944.031
AGTCTAT254.4437103E-544.026
ATAGTCC207.8575086E-444.033
ATACGGA408.3164196E-944.044
TACTCGA1900.042.84210644
AGGGGAT49550.042.756816
TAGGGAT72800.042.700555
ATAGGGG1159950.042.6514934
TAGGGGA342550.042.6256035
TAGGGGG390200.042.62435
TAGGGGC278150.042.560495
AATAGGG1681200.042.4911963
CAATAGG1706300.042.4566572
ACAATAG1726250.042.401851
TAGGGCC15050.042.392035
ATAGGGC111800.042.307694
TAGGGGT151550.042.243485
Produced by FastQC (version 0.11.5)