##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1670525
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.70724772152467	31.0	30.0	33.0	28.0	34.0
2	31.698382245102586	33.0	31.0	34.0	30.0	34.0
3	32.26924829020817	33.0	31.0	34.0	31.0	34.0
4	35.96048547612278	37.0	35.0	37.0	35.0	37.0
5	35.88558267610482	37.0	35.0	37.0	35.0	37.0
6	35.38000808129181	36.0	35.0	37.0	33.0	37.0
7	36.25803025994822	37.0	35.0	37.0	35.0	37.0
8	36.65054159620479	37.0	37.0	37.0	35.0	37.0
9	38.67646578171533	39.0	39.0	39.0	39.0	39.0
10	37.71847532961195	39.0	38.0	39.0	35.0	39.0
11	37.33745199862319	39.0	37.0	39.0	35.0	39.0
12	37.14463477050628	39.0	37.0	39.0	35.0	39.0
13	37.13668218075156	39.0	37.0	39.0	34.0	39.0
14	37.9118791996528	40.0	38.0	40.0	34.0	40.0
15	37.930048936711515	40.0	38.0	40.0	34.0	40.0
16	38.055073105760165	40.0	38.0	40.0	34.0	40.0
17	37.986085212732526	40.0	38.0	40.0	34.0	40.0
18	37.82659523203783	40.0	37.0	40.0	34.0	40.0
19	37.79462145134015	39.0	37.0	40.0	34.0	40.0
20	37.584829320123916	39.0	37.0	40.0	33.0	40.0
21	37.49771179718951	39.0	37.0	40.0	33.0	40.0
22	37.26752009098936	38.0	36.0	40.0	33.0	40.0
23	37.083834124002934	38.0	36.0	40.0	33.0	40.0
24	36.9180580955089	38.0	36.0	40.0	33.0	40.0
25	36.66305323176846	38.0	35.0	40.0	32.0	40.0
26	36.083543796112004	38.0	35.0	39.0	31.0	40.0
27	35.9042157405606	38.0	35.0	39.0	31.0	40.0
28	35.71515661244219	37.0	34.0	39.0	31.0	40.0
29	35.299961389383576	37.0	34.0	38.0	30.0	40.0
30	35.006422531838794	36.0	34.0	38.0	30.0	40.0
31	34.76351147094476	36.0	33.0	38.0	29.0	39.0
32	34.71933853130004	36.0	33.0	38.0	29.0	39.0
33	34.2084386644917	36.0	33.0	38.0	28.0	39.0
34	33.72497268822676	35.0	32.0	38.0	27.0	39.0
35	33.55947142365424	35.0	32.0	38.0	27.0	39.0
36	33.16209754418521	34.0	31.0	37.0	26.0	38.0
37	32.85526645814939	34.0	31.0	37.0	26.0	38.0
38	32.38310710704719	34.0	31.0	37.0	24.0	38.0
39	31.55210846889451	33.0	29.0	36.0	23.0	38.0
40	31.194043190015115	33.0	29.0	36.0	23.0	38.0
41	30.8249538318792	33.0	29.0	35.0	22.0	38.0
42	30.467201029616437	32.0	28.0	35.0	22.0	38.0
43	29.942495921940708	32.0	27.0	34.0	21.0	37.0
44	29.470075575043772	31.0	27.0	34.0	21.0	37.0
45	28.910644258541478	31.0	26.0	34.0	20.0	36.0
46	28.901535146136695	31.0	26.0	34.0	20.0	36.0
47	28.28116131156373	30.0	25.0	33.0	18.0	36.0
48	27.662258870714297	29.0	24.0	33.0	18.0	35.0
49	27.055578336151807	29.0	24.0	32.0	17.0	35.0
50	30.91243052333847	33.0	29.0	35.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	20.0
15	48.0
16	141.0
17	279.0
18	495.0
19	817.0
20	1206.0
21	1975.0
22	3179.0
23	4690.0
24	6984.0
25	9870.0
26	13958.0
27	19535.0
28	26891.0
29	37813.0
30	52341.0
31	70952.0
32	107621.0
33	155769.0
34	240546.0
35	458209.0
36	406509.0
37	49556.0
38	1110.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.59910806482992	0.3324104697624998	0.04298050014217087	0.025500965265410572
8	98.88017240089194	1.0215351461366935	0.06758354409541911	0.030708908875952172
9	98.5005911315305	0.7642507594917765	0.3345056194908786	0.400652489486838
10	68.92336241600694	19.938642043668906	4.2708729291689735	6.8671226111551755
11	35.1709193217701	26.530402119094298	16.11134224270813	22.18733631642747
12	27.66693105460858	18.188054653477202	28.50451205459362	25.6405022373206
13	24.654285329462294	17.10426362969725	30.114484967300697	28.126966073539755
14	24.16731267116625	17.348498226605408	32.86499753071639	25.61919157151195
15	25.144430643061312	15.949596683677287	29.999191870819054	28.906780802442345
16	29.025905029855885	16.95868065428533	25.881384594663352	28.134029721195432
17	30.379730922913456	17.70784633573278	23.09028598793792	28.822136753415844
18	29.702458807859806	18.05713772616393	23.253947112434716	28.986456353541552
19	30.575417907544033	18.642881728797832	22.486643420481585	28.295056943176544
20	28.389458403795214	20.197063797309227	23.641011059397496	27.772466739498064
21	29.544963409706533	18.72848356055731	25.08965744301941	26.63689558671675
22	29.50479639634247	18.24689843013424	24.119662980200836	28.128642193322456
23	29.964053216803098	19.33308391074662	22.106942428278536	28.59592044417174
24	29.812962990676585	19.54457431047126	22.227742775474777	28.41471992337738
25	28.516604061597402	18.90531419763248	22.27395579234073	30.304125948429384
26	29.137845886772123	17.68096855778872	21.836249083371996	31.344936472067165
27	28.90133341314856	17.737657323296567	22.029242304066088	31.33176695948878
28	30.77517546878975	19.862797623501592	19.971326379431616	29.390700528277037
29	30.377276604660214	20.050582900585145	21.09324912826806	28.478891366486586
30	30.378952724442915	19.43335179060475	22.27886442884722	27.908831056105114
31	32.336421184956826	19.94821987099864	19.823588392870505	27.891770551174034
32	34.89022911958815	18.980380419329254	20.290447613774113	25.838942847308484
33	33.852531389832535	18.956256266742493	21.156941680010775	26.034270663414194
34	30.047859205938256	22.20535460409153	23.897996138938357	23.848790051031862
35	30.86275272743599	24.261714131784917	23.61814399664776	21.257389144131338
36	32.10146510827435	23.163077475643885	20.069379386719742	24.666078029362026
37	30.666048098651622	23.7461277143413	20.104218733631644	25.483605453375436
38	34.073719339728534	21.660256506188176	20.267580551024377	23.99844360305892
39	32.48457820146361	20.653327546729322	19.72781011957319	27.134284132233876
40	30.382903578216432	20.729231828317445	21.867796052139298	27.02006854132683
41	27.488843327696383	20.92545756573532	22.690471558342438	28.895227548225854
42	27.91996528037593	19.189057332275784	23.44562338187097	29.44535400547732
43	27.99078134119513	18.01212193771419	25.527184567725712	28.469912153364962
44	27.814130288382394	18.358779425629667	24.59580072133012	29.23128956465782
45	27.383547088490147	18.680953592433518	24.437407401864682	29.498091917211656
46	28.095658550455692	20.032444890079464	23.386480298109877	28.485416261354963
47	28.073749270438935	20.657278400502836	24.86068750841801	26.408284820640215
48	29.204112479609705	21.871148291704703	23.464240283743134	25.460498944942454
49	29.20758444201673	20.54461920653687	23.878660900016463	26.369135451429937
50	26.32058783915236	21.883479744391735	23.533559809042067	28.262372607413837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	4.5
2	9.0
3	13.0
4	17.0
5	17.5
6	18.0
7	348.0
8	678.0
9	719.0
10	760.0
11	1277.5
12	1795.0
13	2127.0
14	2459.0
15	2465.0
16	2471.0
17	2754.0
18	3037.0
19	3736.0
20	4435.0
21	4500.5
22	4566.0
23	5161.5
24	5757.0
25	6056.0
26	6355.0
27	7003.0
28	7651.0
29	9300.5
30	10950.0
31	10563.0
32	10176.0
33	12172.0
34	14168.0
35	15761.0
36	17354.0
37	18970.5
38	20587.0
39	24547.0
40	28507.0
41	29533.0
42	30559.0
43	36605.0
44	42651.0
45	49174.0
46	55697.0
47	64039.0
48	72381.0
49	75102.0
50	77823.0
51	103511.5
52	129200.0
53	150165.5
54	171131.0
55	176534.0
56	181937.0
57	179317.5
58	176698.0
59	170830.5
60	164963.0
61	147587.5
62	130212.0
63	111351.5
64	92491.0
65	76357.0
66	60223.0
67	54519.0
68	48815.0
69	42846.0
70	36877.0
71	36313.0
72	35749.0
73	25401.0
74	15053.0
75	9647.5
76	4242.0
77	2849.5
78	1457.0
79	940.5
80	424.0
81	264.0
82	104.0
83	77.5
84	51.0
85	29.5
86	8.0
87	15.5
88	23.0
89	14.5
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1670525.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.004160644834185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.98529558753597	7.81133596066285
2	9.553128736969112	2.1024832656478005
3	3.4850873921134466	1.1505138457230777
4	1.6674123741264801	0.7339389450428859
5	0.9291483560438925	0.511224888639529
6	0.6213209903804753	0.4102269594092535
7	0.5132063106463902	0.39531832804068884
8	0.4095175289928995	0.3605117340730726
9	0.3221490277983821	0.3190481688123495
>10	7.730020124582794	23.22321795895262
>50	2.4986580320893808	18.808078420248496
>100	1.1586386123282233	24.353535380020563
>500	0.08134654393955268	6.107889656901469
>1k	0.03902435553856919	8.739148077794091
>5k	0.00549638810402383	4.340269958699306
>10k+	5.49638810402383E-4	0.633258451331964
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	10470	0.6267490758893163	No Hit
ACAATAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	9892	0.5921491746606605	No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	9747	0.5834692686430912	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	8568	0.512892653507131	No Hit
ACAATAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	8368	0.5009203693449664	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7433	0.4449499408868469	No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	6100	0.36515466694602	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5741	0.34366441687493454	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	5365	0.3211565226500651	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	5278	0.3159485790395235	No Hit
ACAATAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	5268	0.3153499648314153	No Hit
ACAATAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	4985	0.2984091827419524	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	4766	0.28529953158438215	No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4355	0.26069648763113396	No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	4027	0.241061941605184	No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	3982	0.23836817766869697	No Hit
ACAATAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	3925	0.23495607668248003	No Hit
ACAATAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	3839	0.22980799449274927	No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	3818	0.228550904655722	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3796	0.2272339533978839	No Hit
ACAATAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG	3591	0.2149623621316652	No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	3562	0.21322638092815135	No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	3462	0.20724023884706902	No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	3455	0.20682120890139327	No Hit
ACAATAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3317	0.1985603328294997	No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2830	0.16940782089462894	No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2772	0.1659358584876012	No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	2495	0.14935424492300325	No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2455	0.1469597880905703	No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	2407	0.14408643989165085	No Hit
ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2401	0.14372727136678587	No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	2387	0.14288921147543437	No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	2352	0.14079406174705558	No Hit
ACAATAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTT	2309	0.1382200206521902	No Hit
ACAATAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2076	0.12427230960326842	No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	2035	0.1218179913500247	No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGA	1946	0.11649032489786146	No Hit
ACAATAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT	1910	0.11433531374867184	No Hit
ACAATAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	1871	0.11200071833704973	No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	1807	0.10816958740515706	No Hit
ACAATAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT	1770	0.10595471483515662	No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1738	0.10403914936921027	No Hit
ACAATAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	1706	0.10212358390326395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.06746382125379746	0.0	0.0	0.0	0.0
7	0.06770326693704075	0.0	0.0	0.0	0.0
8	0.06908007961568968	0.0	0.0	0.0	0.0
9	0.07003786234866284	0.0	0.0	0.0	0.0
10	0.08069319525298933	0.0	0.0	0.0	0.0
11	0.10379970368596698	0.0	0.0	0.0	0.0
12	0.11738824621002379	0.0	0.0	0.0	0.0
13	0.12930066895137757	0.0	0.0	0.0	0.0
14	0.14109336885110968	0.0	0.0	0.0	0.0
15	0.151808563176247	0.0	0.0	0.0	0.0
16	0.1620448661348977	0.0	0.0	0.0	0.0
17	0.17072477215246704	0.0	0.0	0.0	0.0
18	0.1807814908486853	0.0	0.0	0.0	0.0
19	0.193053082114904	0.0	0.0	0.0	0.0
20	0.20406758354409543	0.0	0.0	0.0	0.0
21	0.2141841636611245	0.0	0.0	0.0	0.0
22	0.22430074377815357	0.0	0.0	0.0	0.0
23	0.23555469089058828	0.0	0.0	0.0	0.0
24	0.24770655931518534	0.0	0.0	0.0	0.0
25	0.2595591206357283	0.0	0.0	0.0	0.0
26	0.27272863321410934	0.0	0.0	0.0	0.0
27	0.2899088609868155	0.0	0.0	0.0	0.0
28	0.3143921820984421	0.0	0.0	0.0	0.0
29	0.3271426647311474	0.0	0.0	0.0	0.0
30	0.3406713458343934	0.0	0.0	0.0	0.0
31	0.3525239071549363	0.0	0.0	0.0	0.0
32	0.36635189536223645	0.0	0.0	0.0	0.0
33	0.37736639679142786	0.0	0.0	0.0	0.0
34	0.3926909205189985	0.0	0.0	0.0	0.0
35	0.4117268523368402	0.0	0.0	0.0	0.0
36	0.4275901288517083	0.0	0.0	0.0	0.0
37	0.43896379880576464	0.0	0.0	0.0	0.0
38	0.450337468759821	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	25	4.4437103E-5	44.0	37
GACGTAT	20	7.8575086E-4	44.0	9
CGCATGA	20	7.8575086E-4	44.0	40
CCGTAGA	40	8.3164196E-9	44.0	31
AGTCTAT	25	4.4437103E-5	44.0	26
ATAGTCC	20	7.8575086E-4	44.0	33
ATACGGA	40	8.3164196E-9	44.0	44
TACTCGA	190	0.0	42.842106	44
AGGGGAT	4955	0.0	42.75681	6
TAGGGAT	7280	0.0	42.70055	5
ATAGGGG	115995	0.0	42.651493	4
TAGGGGA	34255	0.0	42.625603	5
TAGGGGG	39020	0.0	42.6243	5
TAGGGGC	27815	0.0	42.56049	5
AATAGGG	168120	0.0	42.491196	3
CAATAGG	170630	0.0	42.456657	2
ACAATAG	172625	0.0	42.40185	1
TAGGGCC	1505	0.0	42.39203	5
ATAGGGC	11180	0.0	42.30769	4
TAGGGGT	15155	0.0	42.24348	5
>>END_MODULE