##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1399054
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.964064289155388	33.0	31.0	34.0	30.0	34.0
2	32.619592238755615	34.0	31.0	34.0	31.0	34.0
3	32.86773705661111	34.0	31.0	34.0	31.0	34.0
4	36.36453274855724	37.0	37.0	37.0	35.0	37.0
5	36.3214371997078	37.0	37.0	37.0	35.0	37.0
6	35.851190876120576	37.0	35.0	37.0	35.0	37.0
7	36.47508888148706	37.0	36.0	37.0	35.0	37.0
8	36.7322190565911	37.0	37.0	37.0	35.0	37.0
9	38.78887662663485	39.0	39.0	39.0	39.0	39.0
10	38.04442930723189	39.0	39.0	39.0	35.0	39.0
11	37.86047000330223	39.0	37.0	39.0	35.0	39.0
12	37.67789377679489	39.0	37.0	39.0	35.0	39.0
13	37.680897949614526	39.0	37.0	39.0	35.0	39.0
14	38.42967247868917	40.0	38.0	40.0	35.0	40.0
15	38.554946413791036	40.0	39.0	40.0	35.0	40.0
16	38.66523093461725	40.0	39.0	40.0	35.0	40.0
17	38.614223611097216	40.0	38.0	40.0	35.0	40.0
18	38.519359510068945	40.0	38.0	40.0	35.0	40.0
19	38.441828549862976	40.0	38.0	40.0	35.0	40.0
20	38.3625399734392	40.0	38.0	40.0	35.0	40.0
21	38.35491982439563	40.0	38.0	40.0	35.0	40.0
22	38.298313717697816	40.0	38.0	40.0	35.0	40.0
23	38.196915201271715	40.0	38.0	40.0	35.0	40.0
24	38.14338688856899	40.0	38.0	40.0	34.0	40.0
25	38.15390256559075	40.0	38.0	40.0	35.0	40.0
26	38.093724759730506	40.0	38.0	40.0	35.0	40.0
27	38.01960396096219	40.0	38.0	40.0	35.0	40.0
28	37.87405060848259	40.0	37.0	40.0	34.0	40.0
29	37.790991627199524	40.0	37.0	40.0	34.0	40.0
30	37.646453246264976	40.0	37.0	40.0	34.0	40.0
31	37.57146042969035	40.0	37.0	40.0	33.0	40.0
32	37.46034463287336	40.0	36.0	40.0	33.0	40.0
33	37.2522082778792	39.0	36.0	40.0	33.0	40.0
34	36.83573757696272	39.0	35.0	40.0	32.0	40.0
35	36.72439448370113	39.0	35.0	40.0	31.0	40.0
36	36.64345550636359	39.0	35.0	40.0	31.0	40.0
37	36.44172776747717	38.0	35.0	40.0	31.0	40.0
38	36.54808749340626	39.0	35.0	40.0	31.0	40.0
39	36.451145559785395	39.0	35.0	40.0	31.0	40.0
40	36.133563107642736	38.0	35.0	40.0	30.0	40.0
41	35.99441980080826	38.0	35.0	40.0	30.0	40.0
42	35.871278020719714	38.0	35.0	40.0	30.0	40.0
43	35.834277304521486	38.0	35.0	40.0	30.0	40.0
44	35.672292849311035	38.0	35.0	40.0	30.0	40.0
45	35.5189899746543	37.0	34.0	40.0	30.0	40.0
46	35.35733073919949	37.0	34.0	40.0	30.0	40.0
47	35.10916376351449	36.0	34.0	40.0	29.0	40.0
48	34.88720378198411	36.0	34.0	40.0	28.0	40.0
49	34.68629945663284	36.0	34.0	39.0	28.0	40.0
50	35.08042362910938	36.0	34.0	39.0	30.0	40.0
51	35.14430322203432	36.0	34.0	39.0	30.0	40.0
52	34.77343118993262	35.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	5.0
15	9.0
16	37.0
17	88.0
18	178.0
19	293.0
20	547.0
21	844.0
22	1309.0
23	2091.0
24	3064.0
25	4332.0
26	6057.0
27	8273.0
28	10884.0
29	13490.0
30	17372.0
31	23205.0
32	32926.0
33	45617.0
34	88408.0
35	95481.0
36	160738.0
37	277118.0
38	493925.0
39	112755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.60308894438671	0.33379697995931534	0.03666763398696548	0.026446441667012138
8	98.88503231469265	1.027051135981885	0.059254324707981254	0.028662224617491537
9	98.54830478308915	0.7236318255049483	0.3326533500493905	0.3954100413565166
10	69.36587151032055	19.68094155050484	4.224568887262393	6.72861805191222
11	35.32329702784882	26.39126152385826	16.125253206809745	22.160188241483173
12	27.669410901938026	18.144546243390177	28.659579973324835	25.526462881346966
13	24.873235772171768	17.056954199051646	30.157377770979533	27.912432257797054
14	24.394412224260105	17.219635553738456	32.82375090596932	25.56220131603212
15	25.153067715756507	15.853784056941333	30.112275866406872	28.880872360895292
16	29.14991129720511	16.85253035265258	25.955681481915637	28.041876868226673
17	30.284821029066784	17.684592588992277	23.177089662014474	28.853496719926465
18	29.75267573660488	17.984009194784477	23.30517621192606	28.95813885668459
19	30.817681090222393	18.51865617767434	22.364183226666018	28.299479505437247
20	28.632633193572225	20.0110217332569	23.616600931772467	27.739744141398404
21	29.77218892194297	18.561685253035265	25.009256254583455	26.656869570438314
22	29.752103921649915	18.18021320120596	24.087204639706545	27.980478237437584
23	30.08411397987497	19.306331278135083	22.08106334709025	28.528491394899696
24	30.006204192261343	19.19975926590396	22.295351001462414	28.498685540372282
25	28.70596846154616	18.77297087889388	22.263257887115152	30.25780277244481
26	29.22796403855748	17.448218581984683	21.870563966794705	31.45325341266313
27	29.067712897429267	17.637489332077248	22.097074165829195	31.197723604664297
28	30.838909720425374	19.75141774370396	19.96584835181487	29.4438241840558
29	30.514547687222937	19.816247264222824	21.04379101878841	28.625414029765828
30	30.38517455366269	19.321269943833478	22.333090788489937	27.9604647140139
31	32.42905563330651	19.708889006428628	19.957271127490433	27.904784232774432
32	34.93381956665004	18.91170748234164	20.25854613188626	25.89592681912206
33	33.901979480420344	18.764250700830704	21.216336181448323	26.117433637300635
34	30.2366456191112	22.012659983102868	23.969982573939248	23.780711823846683
35	31.135538728312127	24.013440510516393	23.454920253256844	21.396100507914635
36	32.2309932282814	22.91934407106516	20.136892500218003	24.71277020043544
37	30.907598991890232	23.51503230039727	20.02288689357237	25.55448181414013
38	34.12498731285569	21.45935753730735	20.43473661488406	23.980918534952906
39	32.65863933772392	20.42344326952355	19.788800146384627	27.1291172463679
40	30.606681371841262	20.540164997205252	21.877282792515516	26.97587083843797
41	27.67534348209576	20.60499451772412	22.787397770207583	28.93226422997254
42	28.281753241833414	18.882330489030444	23.62675064722305	29.20916562191309
43	28.225929806855206	17.715256165952137	25.68714288369141	28.371671143501253
44	27.881911634575935	18.050196775821377	24.792038048567104	29.275853541035584
45	27.655615866149557	18.438101745894013	24.410137135521573	29.496145252434857
46	28.32385311789252	19.620329165278825	23.420468402220358	28.6353493146083
47	28.197553489715194	20.381629300941924	24.931203513231086	26.489613696111803
48	29.44861313430361	21.47765561586615	23.709735292561973	25.36399595726827
49	29.35912409385199	20.1689141376959	24.079413660945182	26.39254810750693
50	26.39662228906104	21.668212949607376	23.61381333386703	28.32135142746456
51	26.50348020876964	23.665133726074906	22.014875766053347	27.81651029910211
52	27.91643496248179	22.51692929651036	23.02991878798102	26.53671695302683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	3.5
2	7.0
3	8.5
4	10.0
5	12.0
6	14.0
7	258.5
8	503.0
9	627.0
10	751.0
11	1289.0
12	1827.0
13	1824.0
14	1874.5
15	1928.0
16	2016.5
17	2105.0
18	2210.0
19	2315.0
20	2698.0
21	3081.0
22	3225.5
23	3370.0
24	3912.0
25	4454.0
26	4942.0
27	5430.0
28	6666.5
29	7903.0
30	8324.0
31	8745.0
32	8733.0
33	8721.0
34	10945.5
35	13170.0
36	14616.0
37	16062.0
38	16651.5
39	20263.0
40	23285.0
41	24024.5
42	24764.0
43	31160.0
44	37556.0
45	39359.0
46	41162.0
47	46992.5
48	52823.0
49	63532.0
50	74241.0
51	82489.0
52	90737.0
53	111385.0
54	132033.0
55	144377.5
56	156722.0
57	148616.0
58	140510.0
59	140833.5
60	141157.0
61	124269.0
62	107381.0
63	95058.0
64	68837.5
65	54940.0
66	49518.5
67	44097.0
68	39733.5
69	35370.0
70	34765.0
71	34160.0
72	25410.0
73	16660.0
74	11489.5
75	6319.0
76	3959.5
77	1600.0
78	1270.0
79	940.0
80	568.5
81	197.0
82	162.5
83	128.0
84	93.5
85	59.0
86	34.0
87	9.0
88	8.5
89	5.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1399054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.169931151727653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.03131121292685	6.918737511972234
2	9.700275922733601	1.9730227657392432
3	3.4253111606879374	1.0450553603232198
4	1.6829179250271524	0.68460637726138
5	0.9615477205157532	0.4889437058372936
6	0.6976272343430701	0.4256892565703517
7	0.5433342015467859	0.38679699954768104
8	0.42855330397636016	0.3486686077027996
9	0.4466809784164999	0.4088443317553934
>10	10.193132738798662	27.533011957533095
>50	2.5637594472545175	17.716706547994256
>100	1.188203897669882	22.943176883101422
>500	0.08663253077261143	5.806242155738391
>1k	0.04507708918249701	9.207871015382114
>5k	0.005634636147812126	4.112626523541121
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9716	0.6944692628018647	No Hit
ACAATAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	8381	0.59904764219251	No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8197	0.5858958982283743	No Hit
ACAATAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	7144	0.5106307547814452	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6981	0.4989800250740858	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6314	0.45130495320409364	No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5646	0.4035584044647312	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5036	0.3599575141488463	No Hit
ACAATAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4743	0.3390147914233475	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4433	0.3168569619185535	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4380	0.31306868784192743	No Hit
ACAATAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	4312	0.3082082607247469	No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3767	0.2692533669179317	No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3735	0.266966107098082	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3631	0.25953251268357047	No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3516	0.2513126727059856	No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	3443	0.24609486124195348	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3378	0.2414488647328838	No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3290	0.23515890022829714	No Hit
ACAATAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	3204	0.22901188946245105	No Hit
ACAATAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	3201	0.22879745885434014	No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2943	0.21035642655680195	No Hit
ACAATAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	2897	0.207068490565768	No Hit
ACAATAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2816	0.20127886414677346	No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	2786	0.19913455806566438	No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2426	0.17340288509235527	No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2413	0.17247368579054134	No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2413	0.17247368579054134	No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2379	0.17004347223195102	No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2218	0.15853569626333222	No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2114	0.1511021018488207	No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2024	0.1446691836054934	No Hit
ACAATAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1992	0.1423819237856437	No Hit
ACAATAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTAC	1794	0.1282295036503237	No Hit
ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1761	0.12587076696110372	No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1591	0.1137196991681522	No Hit
ACAATAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1554	0.11107505500145098	No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1542	0.11021733256900733	No Hit
ACAATAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	1534	0.10964551761404491	No Hit
ACAATAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	1516	0.10835893396537948	No Hit
ACAATAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	1495	0.10685791970860309	No Hit
ACAATAGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA	1487	0.10628610475364067	No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAG	1474	0.10535690545182674	No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1461	0.1044277061500128	No Hit
ACAATAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1443	0.10314112250134734	No Hit
ACAATAGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	1428	0.1020689694607928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.07290640675770914	0.0	0.0	0.0	0.0
7	0.07383560605952308	0.0	0.0	0.0	0.0
8	0.07555105092441035	0.0	0.0	0.0	0.0
9	0.07690911144244611	0.0	0.0	0.0	0.0
10	0.08984642479847096	0.0	0.0	0.0	0.0
11	0.11121800874019158	0.0	0.0	0.0	0.0
12	0.12858688799717524	0.0	0.0	0.0	0.0
13	0.1394513721414613	0.0	0.0	0.0	0.0
14	0.15203130115063465	0.0	0.0	0.0	0.0
15	0.1647541838985486	0.0	0.0	0.0	0.0
16	0.17461799187165042	0.0	0.0	0.0	0.0
17	0.18598281410152862	0.0	0.0	0.0	0.0
18	0.19441708468722438	0.0	0.0	0.0	0.0
19	0.20849802795317407	0.0	0.0	0.0	0.0
20	0.2182903590569056	0.0	0.0	0.0	0.0
21	0.22886893572371045	0.0	0.0	0.0	0.0
22	0.23880422056618258	0.0	0.0	0.0	0.0
23	0.25074085775102317	0.0	0.0	0.0	0.0
24	0.2649647547557135	0.0	0.0	0.0	0.0
25	0.27690139194055413	0.0	0.0	0.0	0.0
26	0.28890950599476506	0.0	0.0	0.0	0.0
27	0.30106057378771656	0.0	0.0	0.0	0.0
28	0.32622043180606325	0.0	0.0	0.0	0.0
29	0.3372993465584602	0.0	0.0	0.0	0.0
30	0.35202358164874265	0.0	0.0	0.0	0.0
31	0.36360283448673175	0.0	0.0	0.0	0.0
32	0.3759683328877942	0.0	0.0	0.0	0.0
33	0.3855462333834148	0.0	0.0	0.0	0.0
34	0.3991268385637724	0.0	0.0	0.0	0.0
35	0.42121319119919604	0.0	0.0	0.0	0.0
36	0.43393607394711	0.0	0.0	0.0	0.0
37	0.4461586186094318	0.0	0.0	0.0	0.0
38	0.4576663945780506	0.0	0.0	0.0	0.0
39	0.47010336984848333	0.0	0.0	0.0	0.0
40	0.4833265906819894	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATA	20	6.3119264E-4	46.0	45
GCGGTAT	25	3.4175402E-5	46.0	36
TAGCGTA	20	6.3119264E-4	46.0	24
CGTAGAA	20	6.3119264E-4	46.0	42
AACGACG	20	6.3119264E-4	46.0	24
TTACGTG	25	3.4175402E-5	46.0	46
ATCATAT	20	6.3119264E-4	46.0	29
ACGGTTA	25	3.4175402E-5	46.0	30
ACGAATA	25	3.4175402E-5	46.0	15
TAGGGCC	1335	0.0	44.621723	5
ATAGGGG	97180	0.0	44.59179	4
TAGGGGC	23045	0.0	44.572792	5
TAGGGGG	33440	0.0	44.466206	5
TAGGGGT	12480	0.0	44.451923	5
AATAGGG	140740	0.0	44.429516	3
TAGGGGA	28465	0.0	44.42438	5
CAATAGG	142645	0.0	44.41663	2
TAGGGAT	6245	0.0	44.416332	5
ACAATAG	144310	0.0	44.399837	1
ATAGGGC	9500	0.0	44.16	4
>>END_MODULE