FastQCFastQC Report
Fri 17 Jun 2016
SRR1527481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858355
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA118060.6352930414264228No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG113870.6127462191023783No Hit
ACAATAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA113000.6080646593358104No Hit
ACAATAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA97600.5251956703643814No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT96650.5200836223434167No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC81120.4365150899585924No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG71700.38582509800334164No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC66840.35967293654872184No Hit
ACAATAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT62290.33518891707989057No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC61510.33099165659951946No Hit
ACAATAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG57160.30758385776668073No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT56880.3060771488762911No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC55080.29639116315235786No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG48680.2619521028005951No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT47680.25657099962063223No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT45060.24247250928912936No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC44870.2414500996849364No Hit
ACAATAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT44480.2393514694447509No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG44470.23929765841295123No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC43220.2325712794379976No Hit
ACAATAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA42160.2268673100672369No Hit
ACAATAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG41580.2237462702228584No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG38580.20760296068296963No Hit
ACAATAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG38510.20722628346037222No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT38030.20464335393399002No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG32370.17418630993539985No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG31220.16799804127844248No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT29530.15890397690430513No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA29150.15685915769591924No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT27270.14674268371758895No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA26600.14313734458701377No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC25780.13872483997944418No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAG25690.13824054069324754No Hit
ACAATAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTAC25570.13759480831165197No Hit
ACAATAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG23900.1286083660011139No Hit
ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC22160.11924524646797839No Hit
ACAATAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA21630.11639326178259805No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA21560.11601658456000064No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC21440.11537085217840509No Hit
ACAATAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG20750.11165789098423068No Hit
ACAATAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT20550.1105816703482381No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA19900.1070839532812622No Hit
ACAATAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC19600.10546962232727332No Hit
ACAATAGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT19000.10224096041929556No Hit
ACAATAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT18760.10094949565610446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT206.3124654E-446.033
GTATTAG206.3124654E-446.043
ATATGCG206.3124654E-446.026
TAACGGG405.6152203E-946.040
CGTTATT501.6370905E-1146.033
CCCGTAA301.8620267E-646.026
CGTAATA301.8620267E-646.016
CTAATTG253.4179757E-546.036
AACACGC206.3124654E-446.043
TAGGGCC17800.045.0955055
TAGGGAT78350.044.972565
ATAGGGG1295850.044.6280064
TAGGGGG437250.044.5955435
TAGGGGC313900.044.556555
TAGGGGA381300.044.479945
AATAGGG1871350.044.3948483
CAATAGG1897400.044.3902172
ACAATAG1918450.044.3731161
TAGGGGT166450.044.3694845
AGGGGAT52700.044.2979136
Produced by FastQC (version 0.11.5)