##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527481_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1858355
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.34287743730342	31.0	30.0	33.0	27.0	34.0
2	31.498486564730637	31.0	31.0	33.0	30.0	34.0
3	31.93402121768984	31.0	31.0	34.0	30.0	34.0
4	35.725615396412415	37.0	35.0	37.0	35.0	37.0
5	35.721858310172166	37.0	35.0	37.0	35.0	37.0
6	35.09617322847357	35.0	35.0	37.0	33.0	37.0
7	36.15524590296257	37.0	35.0	37.0	35.0	37.0
8	36.551991949869645	37.0	37.0	37.0	35.0	37.0
9	38.67287574225592	39.0	39.0	39.0	38.0	39.0
10	37.61632949570991	39.0	37.0	39.0	35.0	39.0
11	37.20626414221179	39.0	37.0	39.0	34.0	39.0
12	37.026575116164565	39.0	37.0	39.0	34.0	39.0
13	37.015993714871485	39.0	37.0	39.0	33.0	39.0
14	37.76949506418311	40.0	38.0	40.0	33.0	40.0
15	37.90685364206516	40.0	38.0	40.0	33.0	40.0
16	37.9840326525341	40.0	38.0	40.0	34.0	40.0
17	37.96320778322764	40.0	38.0	40.0	34.0	40.0
18	37.89730595069295	40.0	37.0	40.0	34.0	40.0
19	37.79115508070309	40.0	37.0	40.0	33.0	40.0
20	37.646096682280835	40.0	37.0	40.0	33.0	40.0
21	37.70586190474909	40.0	37.0	40.0	33.0	40.0
22	37.66268124228148	40.0	37.0	40.0	33.0	40.0
23	37.61564932426797	40.0	37.0	40.0	33.0	40.0
24	37.56666837068267	39.0	37.0	40.0	33.0	40.0
25	37.54335097438326	39.0	37.0	40.0	33.0	40.0
26	37.43076107632826	39.0	36.0	40.0	33.0	40.0
27	37.31808508062238	39.0	36.0	40.0	33.0	40.0
28	37.1271280245163	39.0	36.0	40.0	32.0	40.0
29	37.04345133195756	39.0	36.0	40.0	32.0	40.0
30	36.93819695375749	39.0	35.0	40.0	32.0	40.0
31	36.847605543612495	39.0	35.0	40.0	32.0	40.0
32	36.746342329641	39.0	35.0	40.0	31.0	40.0
33	36.592134441481846	38.0	35.0	40.0	31.0	40.0
34	36.27841612609001	38.0	35.0	40.0	31.0	40.0
35	36.1679609116665	38.0	35.0	40.0	30.0	40.0
36	36.10719318967582	38.0	35.0	40.0	30.0	40.0
37	35.959847284291754	38.0	35.0	40.0	30.0	40.0
38	35.84372792066102	38.0	35.0	40.0	30.0	40.0
39	35.63640423923309	38.0	34.0	40.0	30.0	40.0
40	35.777393985540975	38.0	35.0	40.0	30.0	40.0
41	35.85521603783992	38.0	35.0	40.0	30.0	40.0
42	35.90168885923303	38.0	35.0	40.0	30.0	40.0
43	35.85812775277059	38.0	35.0	40.0	30.0	40.0
44	35.72643493842673	38.0	35.0	40.0	30.0	40.0
45	35.600954607704125	37.0	34.0	40.0	30.0	40.0
46	35.339092369326636	37.0	34.0	40.0	30.0	40.0
47	35.19050181477705	36.0	34.0	40.0	29.0	40.0
48	35.04050786851813	36.0	34.0	40.0	29.0	40.0
49	34.82712291246828	36.0	34.0	39.0	29.0	40.0
50	34.69061992999185	35.0	34.0	39.0	29.0	40.0
51	34.50975298045852	35.0	34.0	39.0	28.0	40.0
52	34.053794350379775	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	3.0
14	10.0
15	19.0
16	58.0
17	120.0
18	289.0
19	554.0
20	991.0
21	1554.0
22	2393.0
23	3815.0
24	5635.0
25	8047.0
26	11312.0
27	15398.0
28	19890.0
29	25845.0
30	33173.0
31	42950.0
32	58455.0
33	83680.0
34	138496.0
35	147003.0
36	241496.0
37	391237.0
38	559683.0
39	66246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.61541255572804	0.3315835779493154	0.03347046177936939	0.019533404543265413
8	98.91081090534371	1.0090106572748478	0.05219670084564037	0.027981736535807204
9	98.52062711376459	0.7553992644031953	0.3226509466705769	0.4013226751616349
10	69.09750828017252	19.819894476566642	4.157117450648557	6.9254797926122835
11	34.874499221085316	26.51813028188909	16.09466436714191	22.512706129883686
12	27.344452486204197	18.135824425365442	28.721153923765915	25.798569164664446
13	24.46324841055665	17.024841862830296	30.320902088137093	28.191007638475963
14	24.120795004183808	17.251332495674937	32.90732933158627	25.72054316855499
15	24.86505538500448	15.890451501462316	30.18363014601623	29.06086296751697
16	28.990316704827656	16.792701071646697	25.965760040465895	28.25122218305975
17	30.168024946794343	17.697425949293866	23.115013008816938	29.019536095094857
18	29.558507389599942	17.992310403555834	23.30765650265961	29.141525704184616
19	30.395215123052377	18.52364053154537	22.537674448638715	28.543469896763536
20	28.286522219920307	20.119944789881377	23.67922167723605	27.91431131296227
21	29.57712600660261	18.602957992418027	25.079330913630603	26.740585087348755
22	29.619313855533523	18.081044794993424	24.07683139120351	28.222809958269547
23	29.99157857352336	19.275972567135987	22.10761668249608	28.62483217684457
24	29.810127774295008	19.379343559223074	22.32097742358161	28.48955124290031
25	28.583290060295262	18.788767485222145	22.23665553675159	30.391286917731
26	29.13022538750669	17.56612703170277	21.771943466129994	31.531704114660545
27	28.94629928081556	17.72965875734184	22.014685030578118	31.309356931264475
28	30.817093612361468	19.6953219379505	20.02050200311566	29.467082446572373
29	30.33091094005182	19.887534943538775	21.15273992321166	28.628814193197748
30	30.272633592612824	19.32929929964942	22.217821675621718	28.180245432116035
31	32.29054728509892	19.816558192595064	19.843732763653875	28.04916175865214
32	34.92906360733014	18.92329506472122	20.288588563541413	25.85905276440723
33	33.798709073347126	18.785754067441367	21.192237220552585	26.22329963865892
34	30.041461400001612	22.140979522211847	23.904743711508296	23.912815366278238
35	30.986598362530305	24.087539786531636	23.571384369509595	21.354477481428468
36	32.01298998307643	23.048717817639794	20.0432102585351	24.89508194074867
37	30.785022237408892	23.68363418184362	20.05364959870423	25.47769398204326
38	34.08164747854958	21.48227868195259	20.308552456339076	24.12752138315876
39	32.2933992697843	20.556244635712766	19.83894358182371	27.311412512679222
40	30.48464905790336	20.583096340580784	21.868695701305725	27.063558900210133
41	27.521706024952174	20.741139340976293	22.804523355333075	28.93263127873845
42	27.917647596933847	19.041840767775803	23.527582189624695	29.51292944566565
43	28.01477650933218	17.890768986549933	25.62292995687046	28.47152454724743
44	27.8374691595524	18.19318698526385	24.649596013678764	29.319747841504988
45	27.307107630135253	18.451802804092868	24.530942688560582	29.710146877211297
46	28.252675080918337	19.807948427507124	23.45875787995297	28.480618611621566
47	28.154093270661416	20.435815546545196	24.905628902981398	26.504462279811985
48	29.359998493291112	21.54286990375897	23.55007520091694	25.54705640203298
49	29.285308781153223	20.255817645175437	23.992079016119096	26.46679455755224
50	26.302132800245374	21.818759063795667	23.582469442060315	28.296638693898636
51	26.36014109252538	23.58063986697913	22.00564477723578	28.053574263259712
52	27.79824091737047	22.554517301592	22.981830705112856	26.665411075924677
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	5.5
2	11.0
3	15.5
4	20.0
5	22.0
6	24.0
7	349.5
8	675.0
9	754.0
10	833.0
11	1514.0
12	2195.0
13	2371.0
14	2599.5
15	2652.0
16	2742.0
17	2832.0
18	3096.0
19	3360.0
20	3859.5
21	4359.0
22	4594.5
23	4830.0
24	5378.5
25	5927.0
26	6621.5
27	7316.0
28	8843.5
29	10371.0
30	10901.0
31	11431.0
32	11452.0
33	11473.0
34	14435.5
35	17398.0
36	19143.0
37	20888.0
38	22028.5
39	27286.5
40	31404.0
41	32314.5
42	33225.0
43	42156.0
44	51087.0
45	53258.5
46	55430.0
47	63453.5
48	71477.0
49	85030.5
50	98584.0
51	110193.5
52	121803.0
53	147800.5
54	173798.0
55	190879.0
56	207960.0
57	197132.5
58	186305.0
59	185894.0
60	185483.0
61	163732.5
62	141982.0
63	126117.5
64	91604.5
65	72956.0
66	65677.5
67	58399.0
68	52673.5
69	46948.0
70	46183.0
71	45418.0
72	33693.5
73	21969.0
74	14979.5
75	7990.0
76	4966.0
77	1942.0
78	1529.5
79	1117.0
80	663.0
81	209.0
82	189.5
83	170.0
84	119.5
85	69.0
86	52.0
87	35.0
88	28.0
89	15.5
90	10.0
91	5.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1858355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.58025027472597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.56768223457705	6.664758068398556
2	9.907893379669392	1.8984019654506663
3	3.7645597049744746	1.0819627244341208
4	1.8267772020392623	0.7000393116679912
5	1.0139401986060246	0.48569004331255355
6	0.6677096933719404	0.3838095584018235
7	0.4952891930492201	0.3321496099445016
8	0.38577513634904004	0.29566578847922714
9	0.3295257312829556	0.28412450798875305
>10	7.387904628113599	19.785685661828783
>50	3.0649443344132514	20.31102772395118
>100	1.4341030691092727	26.301347049486235
>500	0.0986509579772495	6.570287223452179
>1k	0.04791617958894975	9.22494054518581
>5k	0.005637197598699972	3.817289405759298
>10k+	0.0016911592796099914	1.8628208122583287
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	11806	0.6352930414264228	No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	11387	0.6127462191023783	No Hit
ACAATAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	11300	0.6080646593358104	No Hit
ACAATAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	9760	0.5251956703643814	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9665	0.5200836223434167	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	8112	0.4365150899585924	No Hit
ACAATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	7170	0.38582509800334164	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6684	0.35967293654872184	No Hit
ACAATAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	6229	0.33518891707989057	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	6151	0.33099165659951946	No Hit
ACAATAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	5716	0.30758385776668073	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	5688	0.3060771488762911	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	5508	0.29639116315235786	No Hit
ACAATAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4868	0.2619521028005951	No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4768	0.25657099962063223	No Hit
ACAATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4506	0.24247250928912936	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4487	0.2414500996849364	No Hit
ACAATAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4448	0.2393514694447509	No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	4447	0.23929765841295123	No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	4322	0.2325712794379976	No Hit
ACAATAGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	4216	0.2268673100672369	No Hit
ACAATAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	4158	0.2237462702228584	No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3858	0.20760296068296963	No Hit
ACAATAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	3851	0.20722628346037222	No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3803	0.20464335393399002	No Hit
ACAATAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3237	0.17418630993539985	No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3122	0.16799804127844248	No Hit
ACAATAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2953	0.15890397690430513	No Hit
ACAATAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2915	0.15685915769591924	No Hit
ACAATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2727	0.14674268371758895	No Hit
ACAATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2660	0.14313734458701377	No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2578	0.13872483997944418	No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACAATAGGGAAG	2569	0.13824054069324754	No Hit
ACAATAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTAC	2557	0.13759480831165197	No Hit
ACAATAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2390	0.1286083660011139	No Hit
ACAATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2216	0.11924524646797839	No Hit
ACAATAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	2163	0.11639326178259805	No Hit
ACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	2156	0.11601658456000064	No Hit
ACAATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	2144	0.11537085217840509	No Hit
ACAATAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	2075	0.11165789098423068	No Hit
ACAATAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	2055	0.1105816703482381	No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1990	0.1070839532812622	No Hit
ACAATAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1960	0.10546962232727332	No Hit
ACAATAGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	1900	0.10224096041929556	No Hit
ACAATAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1876	0.10094949565610446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.06209793069677214	0.0	0.0	0.0	0.0
7	0.06274366307836769	0.0	0.0	0.0	0.0
8	0.06435799403235658	0.0	0.0	0.0	0.0
9	0.06564945879554768	0.0	0.0	0.0	0.0
10	0.07495876729688353	0.0	0.0	0.0	0.0
11	0.0955145814443419	0.0	0.0	0.0	0.0
12	0.10902115042604885	0.0	0.0	0.0	0.0
13	0.11709280519599323	0.0	0.0	0.0	0.0
14	0.1295231535417076	0.0	0.0	0.0	0.0
15	0.139478194424639	0.0	0.0	0.0	0.0
16	0.14884131395777447	0.0	0.0	0.0	0.0
17	0.15788156730011219	0.0	0.0	0.0	0.0
18	0.16584560000645732	0.0	0.0	0.0	0.0
19	0.1775764049387765	0.0	0.0	0.0	0.0
20	0.18742382375810865	0.0	0.0	0.0	0.0
21	0.19603358884604932	0.0	0.0	0.0	0.0
22	0.20415905464779335	0.0	0.0	0.0	0.0
23	0.21610510370731104	0.0	0.0	0.0	0.0
24	0.22805115276682872	0.0	0.0	0.0	0.0
25	0.2373604612681646	0.0	0.0	0.0	0.0
26	0.24779980143729266	0.0	0.0	0.0	0.0
27	0.26103731526000146	0.0	0.0	0.0	0.0
28	0.28234648385265465	0.0	0.0	0.0	0.0
29	0.2920324695765879	0.0	0.0	0.0	0.0
30	0.3065614481624878	0.0	0.0	0.0	0.0
31	0.31753889864961216	0.0	0.0	0.0	0.0
32	0.3300230580271261	0.0	0.0	0.0	0.0
33	0.3386328231150668	0.0	0.0	0.0	0.0
34	0.3514936597151782	0.0	0.0	0.0	0.0
35	0.3716189856082395	0.0	0.0	0.0	0.0
36	0.3846412553037498	0.0	0.0	0.0	0.0
37	0.39626443817246976	0.0	0.0	0.0	0.0
38	0.40734951072319336	0.0	0.0	0.0	0.0
39	0.41843458327391697	0.0	0.0	0.0	0.0
40	0.4304882543970339	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	20	6.3124654E-4	46.0	33
GTATTAG	20	6.3124654E-4	46.0	43
ATATGCG	20	6.3124654E-4	46.0	26
TAACGGG	40	5.6152203E-9	46.0	40
CGTTATT	50	1.6370905E-11	46.0	33
CCCGTAA	30	1.8620267E-6	46.0	26
CGTAATA	30	1.8620267E-6	46.0	16
CTAATTG	25	3.4179757E-5	46.0	36
AACACGC	20	6.3124654E-4	46.0	43
TAGGGCC	1780	0.0	45.095505	5
TAGGGAT	7835	0.0	44.97256	5
ATAGGGG	129585	0.0	44.628006	4
TAGGGGG	43725	0.0	44.595543	5
TAGGGGC	31390	0.0	44.55655	5
TAGGGGA	38130	0.0	44.47994	5
AATAGGG	187135	0.0	44.394848	3
CAATAGG	189740	0.0	44.390217	2
ACAATAG	191845	0.0	44.373116	1
TAGGGGT	16645	0.0	44.369484	5
AGGGGAT	5270	0.0	44.297913	6
>>END_MODULE