FastQCFastQC Report
Fri 17 Jun 2016
SRR1527477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622165
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41580.6683114607861258No Hit
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT35590.5720347496242958No Hit
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC30100.4837944918148722No Hit
TTGGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG25780.4143595348500799No Hit
TTGGACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG23910.38430319931207957No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC23870.38366028304388705No Hit
TTGGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA23780.3822137214404539No Hit
TTGGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA22310.3585865485843787No Hit
TTGGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA17960.288669404418442No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT17900.2877050300161533No Hit
TTGGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA17760.28545482307747944No Hit
TTGGACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG17520.28159732546832433No Hit
TTGGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC17250.2772576406580248No Hit
TTGGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG17240.2770969115909767No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA16210.2605418176850193No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT14400.23144985654930766No Hit
TTGGACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG14270.22936037867768194No Hit
TTGGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC13680.21987736372184227No Hit
TTGGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT12580.20219716634654797No Hit
TTGGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG12050.19367852579299702No Hit
TTGGACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG11790.18949957004974566No Hit
TTGGACGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT9780.15719302757307146No Hit
TTGGACGGGATGTATATGTGTGCATTTGTCAGTACTGTGTGTGTGTCTTT9690.1557464659696383No Hit
TTGGACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT9620.15462136250030137No Hit
TTGGACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC9560.1536569880980126No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG9200.14787074168427988No Hit
TTGGACGGGACTATATATAATTATTTCTAAAGCACAGATTAAGTAAGTTC7800.12536867229754164No Hit
TTGGACGGGGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAG7640.12279700722477158No Hit
TTGGACGGGACTCTGGGGTGGTGCTTAGCCGGCGCCAGACCGACCCTCGG7500.12054680028609774No Hit
TTGGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC7350.11813586428037579No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC7330.11781440614627953No Hit
TTGGACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT6810.10945649465977675No Hit
TTGGACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC6570.10559899705062162No Hit
TTGGACGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCT6520.10479535171538097No Hit
TTGGACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC6500.1044738935812847No Hit
TTGGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC6430.1033487901119478No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC6380.10254514477670713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT650.044.00000440
CGGGTTA551.8189894E-1244.06
TTAGGTA254.4404787E-544.034
TGATATA850.044.039
TAATAGG207.8536937E-444.027
TCGTTAT254.4404787E-544.044
GCCTTAT302.5258378E-644.020
GGTACCG302.5258378E-644.09
CGAACGT302.5258378E-644.038
CGAGTTA207.8536937E-444.030
AATCAAT408.301868E-944.014
CGAAAAA254.4404787E-544.034
GACGTAA207.8536937E-444.042
CGTTGGC302.5258378E-644.033
TTATGCA351.4446596E-744.016
AACCACT207.8536937E-444.014
AAGACGT254.4404787E-544.020
CAACCGT207.8536937E-444.039
ACCGAAT302.5258378E-644.037
ATTCGTC207.8536937E-444.035
Produced by FastQC (version 0.11.5)