##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622165
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314161034452276	31.0	31.0	33.0	30.0	34.0
2	31.299100720869866	33.0	31.0	34.0	28.0	34.0
3	32.05741081545892	34.0	31.0	34.0	30.0	34.0
4	36.00938014835293	37.0	35.0	37.0	35.0	37.0
5	34.27046844486591	35.0	35.0	37.0	32.0	37.0
6	35.62454172124758	36.0	35.0	37.0	35.0	37.0
7	36.075041186823434	37.0	35.0	37.0	35.0	37.0
8	36.493357871304234	37.0	37.0	37.0	35.0	37.0
9	38.51689182130142	39.0	39.0	39.0	37.0	39.0
10	37.19427483063175	39.0	37.0	39.0	34.0	39.0
11	36.82078066107865	38.0	37.0	39.0	32.0	39.0
12	36.63248012986909	38.0	35.0	39.0	33.0	39.0
13	36.82260975786166	39.0	37.0	39.0	33.0	39.0
14	37.677816977811354	39.0	37.0	40.0	33.0	40.0
15	37.7189186148369	40.0	37.0	40.0	33.0	40.0
16	37.85926241431132	40.0	37.0	40.0	34.0	40.0
17	37.76005882683854	40.0	37.0	40.0	33.0	40.0
18	37.6013340512565	39.0	37.0	40.0	33.0	40.0
19	37.52563708983951	39.0	37.0	40.0	33.0	40.0
20	37.31243158969084	39.0	36.0	40.0	33.0	40.0
21	37.214777430424405	39.0	36.0	40.0	33.0	40.0
22	37.005721954786914	38.0	36.0	40.0	33.0	40.0
23	36.77655605828036	38.0	35.0	40.0	32.0	40.0
24	36.632767834899106	38.0	35.0	40.0	32.0	40.0
25	36.40902011524274	38.0	35.0	40.0	31.0	40.0
26	35.84971992960067	38.0	35.0	39.0	30.0	40.0
27	35.68768252794677	38.0	34.0	39.0	30.0	40.0
28	35.48922552699043	37.0	34.0	39.0	30.0	40.0
29	35.126209285318204	37.0	34.0	38.0	30.0	40.0
30	34.744807245666344	36.0	33.0	38.0	29.0	40.0
31	34.64904808210041	36.0	33.0	38.0	29.0	40.0
32	34.51580850738952	36.0	33.0	38.0	29.0	39.0
33	33.9191147042987	35.0	32.0	38.0	27.0	39.0
34	33.401960894617986	35.0	32.0	38.0	26.0	39.0
35	33.32512918598764	35.0	31.0	38.0	26.0	39.0
36	32.91562366896241	34.0	31.0	37.0	26.0	38.0
37	32.70100053844237	34.0	31.0	37.0	26.0	38.0
38	32.173206464523076	34.0	30.0	36.0	24.0	38.0
39	31.39533082060225	33.0	29.0	36.0	23.0	38.0
40	31.07627076418635	33.0	29.0	35.0	23.0	38.0
41	30.839722581630276	32.0	29.0	35.0	23.0	38.0
42	30.429567719174173	32.0	28.0	35.0	22.0	37.0
43	30.013766444592672	31.0	28.0	34.0	21.0	37.0
44	29.493515385789944	31.0	27.0	34.0	21.0	36.0
45	29.08633240378356	31.0	26.0	34.0	20.0	36.0
46	28.84234407271383	31.0	26.0	34.0	20.0	36.0
47	28.404910272998322	30.0	26.0	33.0	20.0	36.0
48	27.725744778314436	29.0	24.0	33.0	18.0	35.0
49	26.941177983332395	29.0	23.0	32.0	17.0	34.0
50	30.544972796605403	32.0	28.0	34.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	12.0
13	20.0
14	37.0
15	53.0
16	98.0
17	163.0
18	255.0
19	429.0
20	747.0
21	1110.0
22	1716.0
23	2313.0
24	3381.0
25	4625.0
26	6222.0
27	8447.0
28	11199.0
29	14700.0
30	19600.0
31	28139.0
32	44440.0
33	66448.0
34	94113.0
35	151317.0
36	139314.0
37	22883.0
38	376.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.24939525688524	0.5910007795359752	0.10270587384375528	0.05689808973503813
8	99.50254353748603	0.3600331101878119	0.08438276020026841	0.053040592125883
9	98.24403494249918	1.1096734789002918	0.17680197375294335	0.4694896048475887
10	64.61806755442689	26.04437729541199	4.082196844888414	5.255358305272717
11	34.51946027179285	24.966688900854276	20.591161508603022	19.92268931874985
12	29.332572549082634	16.40063327252417	27.761124460553066	26.505669717840124
13	23.202848119068094	17.84430175275048	29.379987623861837	29.57286250431959
14	24.93261433864007	17.901199842485514	30.880554193823183	26.28563162505123
15	25.33588356786383	19.058449125232052	26.907813843594546	28.697853463309574
16	31.216638673020825	18.141168339588372	24.456374112976462	26.185818874414345
17	31.110236030634958	20.71861965877219	22.336357718611623	25.834786591981228
18	29.889820224538504	20.471579082719217	22.45690451889772	27.18169617384456
19	30.95850779134153	19.292952834055274	21.980985751368205	27.767553623234992
20	30.81706621233917	20.07152443483642	22.830278141650524	26.281131211173886
21	31.953259987302403	18.395441723658514	24.574188519122742	25.07710976991634
22	32.34833203410671	17.838033319135597	23.773275578021906	26.040359068735786
23	31.783047905298435	18.907685260340905	22.784309628474762	26.524957205885897
24	30.338415050669838	19.656843441852242	23.06124581099869	26.943495696479232
25	27.714191572975018	20.35183592776836	23.44056640923228	28.493406090024347
26	28.236882499015536	22.02261457973367	22.123552433839897	27.616950487410897
27	29.199488881566786	18.951403566577998	21.79437930452533	30.054728247329887
28	29.55775397201707	20.436379417035674	21.155802721143104	28.85006388980415
29	29.920198018210602	19.853415090852106	21.47758231337346	28.74880457756383
30	28.278672056448052	20.28368680333995	22.465905346652416	28.97173579355959
31	33.50027725764066	20.210715806900097	20.33881687333746	25.950190062121788
32	34.16826726029269	19.222392773621145	21.293226073469256	25.316113892616908
33	32.002121623685035	19.424268481833597	22.55993185087557	26.013678043605797
34	28.809077977706878	23.322430544951903	24.272017873072254	23.596473604268965
35	31.213906278881005	23.465800872758834	22.869978221211415	22.450314627148746
36	30.415243544718845	24.124950776723217	20.506296561201612	24.95350911735633
37	30.9694373679008	23.198347705190745	20.558372778925204	25.27384214798325
38	32.14404538988854	23.6493534673278	21.259794427523246	22.946806715260422
39	32.525455465993744	20.91888807631416	21.437721504745525	25.117934952946563
40	29.583470622744773	20.90458318934688	22.08738839375407	27.424557794154286
41	28.23479302114391	22.170646050484997	21.95044722862906	27.64411369974203
42	27.49817170686233	19.19297935435134	23.532021248382666	29.776827690403675
43	27.060667186357318	18.55472422910321	25.886702080637775	28.4979065039017
44	26.99075004219138	19.30195366180997	25.08225310006188	28.625043195936765
45	28.303102874639364	18.878754028272244	25.13465077591957	27.683492321168824
46	28.742536143948954	19.626787106314243	23.686964069017062	27.943712680719745
47	27.768357268570238	20.45293451094163	24.59829787917996	27.180410341308175
48	28.36096533877669	20.878545080485083	24.87587697797208	25.88461260276615
49	28.349232116882177	19.83219885400175	24.852571263250102	26.965997765865968
50	25.476039314329803	22.17836104570331	24.61195984987905	27.73363979008784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	1.5
8	0.0
9	21.0
10	42.0
11	51.0
12	60.0
13	63.0
14	66.0
15	109.5
16	153.0
17	272.5
18	392.0
19	291.5
20	191.0
21	249.0
22	307.0
23	606.5
24	906.0
25	1118.0
26	1330.0
27	1449.0
28	1568.0
29	2324.5
30	3081.0
31	2806.5
32	2532.0
33	3371.5
34	4211.0
35	4704.5
36	5198.0
37	6383.5
38	7569.0
39	8576.5
40	9584.0
41	10052.0
42	10520.0
43	11017.0
44	11514.0
45	14684.5
46	17855.0
47	18444.5
48	19034.0
49	22486.0
50	25938.0
51	27641.5
52	29345.0
53	31958.0
54	34571.0
55	39355.0
56	44139.0
57	53069.5
58	62000.0
59	62076.5
60	62153.0
61	61998.0
62	61843.0
63	60372.5
64	58902.0
65	53086.0
66	47270.0
67	40195.0
68	33120.0
69	27187.0
70	21254.0
71	18938.0
72	16622.0
73	13844.0
74	11066.0
75	11394.0
76	11722.0
77	8006.0
78	4290.0
79	2800.5
80	1311.0
81	811.0
82	311.0
83	208.0
84	105.0
85	79.5
86	54.0
87	43.5
88	33.0
89	16.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	622165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.704772849645995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02257528528075	8.430078837607386
2	9.667275448690662	2.263065264037675
3	3.4165030278895405	1.1996817564472448
4	1.4734355904041307	0.6898491557705754
5	0.7978248630240445	0.4669179397748186
6	0.5588893618774288	0.3925003817315342
7	0.3460445189019952	0.2835260742729019
8	0.2622797742471472	0.24559401444954315
9	0.2540406190351949	0.26761389663513696
>10	6.7464949260535825	22.487603770703913
>50	3.0855636268761244	24.797119735118496
>100	1.2894277906705298	27.734121977288982
>500	0.05080812380703899	3.870677392653074
>1k	0.028837043241832935	6.871649803508715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4158	0.6683114607861258	No Hit
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3559	0.5720347496242958	No Hit
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3010	0.4837944918148722	No Hit
TTGGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2578	0.4143595348500799	No Hit
TTGGACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2391	0.38430319931207957	No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2387	0.38366028304388705	No Hit
TTGGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2378	0.3822137214404539	No Hit
TTGGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	2231	0.3585865485843787	No Hit
TTGGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1796	0.288669404418442	No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1790	0.2877050300161533	No Hit
TTGGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1776	0.28545482307747944	No Hit
TTGGACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1752	0.28159732546832433	No Hit
TTGGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1725	0.2772576406580248	No Hit
TTGGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1724	0.2770969115909767	No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1621	0.2605418176850193	No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1440	0.23144985654930766	No Hit
TTGGACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG	1427	0.22936037867768194	No Hit
TTGGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1368	0.21987736372184227	No Hit
TTGGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1258	0.20219716634654797	No Hit
TTGGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1205	0.19367852579299702	No Hit
TTGGACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1179	0.18949957004974566	No Hit
TTGGACGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT	978	0.15719302757307146	No Hit
TTGGACGGGATGTATATGTGTGCATTTGTCAGTACTGTGTGTGTGTCTTT	969	0.1557464659696383	No Hit
TTGGACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	962	0.15462136250030137	No Hit
TTGGACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	956	0.1536569880980126	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	920	0.14787074168427988	No Hit
TTGGACGGGACTATATATAATTATTTCTAAAGCACAGATTAAGTAAGTTC	780	0.12536867229754164	No Hit
TTGGACGGGGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAG	764	0.12279700722477158	No Hit
TTGGACGGGACTCTGGGGTGGTGCTTAGCCGGCGCCAGACCGACCCTCGG	750	0.12054680028609774	No Hit
TTGGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	735	0.11813586428037579	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	733	0.11781440614627953	No Hit
TTGGACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	681	0.10945649465977675	No Hit
TTGGACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	657	0.10559899705062162	No Hit
TTGGACGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCT	652	0.10479535171538097	No Hit
TTGGACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	650	0.1044738935812847	No Hit
TTGGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	643	0.1033487901119478	No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	638	0.10254514477670713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.007072078950117734	0.0	0.0	0.0	0.0
8	0.007072078950117734	0.0	0.0	0.0	0.0
9	0.007072078950117734	0.0	0.0	0.0	0.0
10	0.011251034693369123	0.0	0.0	0.0	0.0
11	0.018323113643486856	0.0	0.0	0.0	0.0
12	0.02603810886179711	0.0	0.0	0.0	0.0
13	0.03439602034829989	0.0	0.0	0.0	0.0
14	0.04130737023136949	0.0	0.0	0.0	0.0
15	0.05014746891901666	0.0	0.0	0.0	0.0
16	0.05721954786913439	0.0	0.0	0.0	0.0
17	0.06638110469087782	0.0	0.0	0.0	0.0
18	0.07602484871376564	0.0	0.0	0.0	0.0
19	0.08422203113322028	0.0	0.0	0.0	0.0
20	0.0906511938151455	0.0	0.0	0.0	0.0
21	0.09724108556411884	0.0	0.0	0.0	0.0
22	0.10254514477670715	0.0	0.0	0.0	0.0
23	0.10736701678815105	0.0	0.0	0.0	0.0
24	0.11299253413483562	0.0	0.0	0.0	0.0
25	0.11861805148152017	0.0	0.0	0.0	0.0
26	0.1255294013645898	0.0	0.0	0.0	0.0
27	0.13115491871127435	0.0	0.0	0.0	0.0
28	0.13710189419205515	0.0	0.0	0.0	0.0
29	0.14192376620349909	0.0	0.0	0.0	0.0
30	0.15237115556162753	0.0	0.0	0.0	0.0
31	0.1607290670481303	0.0	0.0	0.0	0.0
32	0.17085499827216252	0.0	0.0	0.0	0.0
33	0.1750339540154139	0.0	0.0	0.0	0.0
34	0.18467769803830172	0.0	0.0	0.0	0.0
35	0.19319633859185265	0.0	0.0	0.0	0.0
36	0.20123279194425917	0.0	0.0	0.0	0.0
37	0.20910851622961754	0.0	0.0	0.0	0.0
38	0.2163413242467834	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	65	0.0	44.000004	40
CGGGTTA	55	1.8189894E-12	44.0	6
TTAGGTA	25	4.4404787E-5	44.0	34
TGATATA	85	0.0	44.0	39
TAATAGG	20	7.8536937E-4	44.0	27
TCGTTAT	25	4.4404787E-5	44.0	44
GCCTTAT	30	2.5258378E-6	44.0	20
GGTACCG	30	2.5258378E-6	44.0	9
CGAACGT	30	2.5258378E-6	44.0	38
CGAGTTA	20	7.8536937E-4	44.0	30
AATCAAT	40	8.301868E-9	44.0	14
CGAAAAA	25	4.4404787E-5	44.0	34
GACGTAA	20	7.8536937E-4	44.0	42
CGTTGGC	30	2.5258378E-6	44.0	33
TTATGCA	35	1.4446596E-7	44.0	16
AACCACT	20	7.8536937E-4	44.0	14
AAGACGT	25	4.4404787E-5	44.0	20
CAACCGT	20	7.8536937E-4	44.0	39
ACCGAAT	30	2.5258378E-6	44.0	37
ATTCGTC	20	7.8536937E-4	44.0	35
>>END_MODULE