FastQCFastQC Report
Fri 17 Jun 2016
SRR1527476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524362
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29880.5698353427593914No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC29660.5656397679465712No Hit
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT24760.4721928743883042No Hit
TTGGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG22750.43386057723481103No Hit
TTGGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG22180.42299022431068617No Hit
TTGGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA20500.39095128937642315No Hit
TTGGACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA20360.3882813781319012No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC19780.37722031726173905No Hit
TTGGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG16460.3139052791773622No Hit
TTGGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC15360.29292740511326143No Hit
TTGGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG15280.29140174154496323No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15020.28644333494799396No Hit
TTGGACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG14610.2786243091604655No Hit
TTGGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14530.27709864559216724No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC13280.2532601523375073No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT13250.2526880284993955No Hit
TTGGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG12460.23762210076245038No Hit
TTGGACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA11510.21950484588890878No Hit
TTGGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT11190.21340219161571586No Hit
TTGGACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10400.1983362638787708No Hit
TTGGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT10360.19757343209462164No Hit
TTGGACGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG8720.1662973289445078No Hit
TTGGACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC8500.16210175413168765No Hit
TTGGACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT8320.15866901110301662No Hit
TTGGACGGGATGTATATGTGTGCATTTGTCAGTACTGTGTGTGTGTCTTTGT7780.14837078201700354No Hit
TTGGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT7040.1342583940102448No Hit
TTGGACGGGGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCA6800.1296814033053501No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT6650.12682078411479092No Hit
TTGGACGGGACTATATATAATTATTTCTAAAGCACAGATTAAGTAAGTTCTG6580.12548582849252998No Hit
TTGGACGGGACTCTGGGGTGGTGCTTAGCCGGCGCCAGACCGACCCTCGGCT6400.12205308546385893No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC6300.12014600600348613No Hit
TTGGACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT6080.115950431190666No Hit
TTGGACGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG6070.1157597232446287No Hit
TTGGACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA6060.11556901529859143No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA6030.11499689146047959No Hit
TTGGACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG5810.11080131664765944No Hit
TTGGACGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCT5640.1075592815650257No Hit
TTGGACGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG5550.10584291005069017No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT5520.10527078621257832No Hit
TTGGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA5470.10431724648239193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTC551.8189894E-1246.00000436
CGTTAGA551.8189894E-1246.00000429
ACGGATC551.8189894E-1246.0000045
TCGCCTT206.308299E-446.040
GTGTTTA405.6006684E-946.020
ACTATAT800.046.010
ACACGTT206.308299E-446.010
TCGCAAT253.4145974E-546.043
CGAACGT206.308299E-446.038
CGAACAA253.4145974E-546.025
AATTTAC301.8594528E-646.038
CGTATAT453.092282E-1046.040
GGTCGAC206.308299E-446.09
CTATCGG206.308299E-446.010
TTAGCGA206.308299E-446.021
GACGTTT206.308299E-446.031
TAGGTAT206.308299E-446.043
CTATAGG206.308299E-446.018
GACCGTT206.308299E-446.09
ATTAAGT850.046.038
Produced by FastQC (version 0.11.5)