FastQCFastQC Report
Fri 17 Jun 2016
SRR1527475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680533
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG42960.6312699016800067No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC39360.5783701892487212No Hit
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT34240.5031350426797819No Hit
TTGGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG29090.42745906517391513No Hit
TTGGACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA27190.3995397725018478No Hit
TTGGACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA26970.3963070122977137No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC26210.3851392952288868No Hit
TTGGACGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG25660.3770573947185515No Hit
TTGGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC20980.30828776855788037No Hit
TTGGACGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG20940.3076999939753105No Hit
TTGGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG19920.2927117421197797No Hit
TTGGACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG19820.29124230566335507No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC18940.2783112648468186No Hit
TTGGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18930.2781643212011761No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC18050.26523328038463967No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT16340.2401059169797791No Hit
TTGGACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG16260.23893036781463942No Hit
TTGGACGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA15060.22129713033754425No Hit
TTGGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT14990.22026852481804704No Hit
TTGGACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT14030.2061619348363709No Hit
TTGGACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG13830.20322306192352171No Hit
TTGGACGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG11220.16487077041083972No Hit
TTGGACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC10770.15825830635692906No Hit
TTGGACGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT10560.1551724897984374No Hit
TTGGACGGGGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCA9050.132983999306426No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT8930.13122067555871647No Hit
TTGGACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT8540.12548987337866055No Hit
TTGGACGGGACTATATATAATTATTTCTAAAGCACAGATTAAGTAAGTTCTG8450.1241673805678784No Hit
TTGGACGGGATGTATATGTGTGCATTTGTCAGTACTGTGTGTGTGTCTTTGT8320.12225711317452645No Hit
TTGGACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT8020.11784880380525264No Hit
TTGGACGGGACTCTGGGGTGGTGCTTAGCCGGCGCCAGACCGACCCTCGGCT7970.11711408557704035No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC7930.11652631099447051No Hit
TTGGACGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCT7900.11608548005754313No Hit
TTGGACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA7730.11358743808162132No Hit
TTGGACGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG7680.11285271985340903No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT7270.10682803038206817No Hit
TTGGACGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG7110.10447693205178882No Hit
TTGGACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG7010.10300749559536422No Hit
TTGGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA7000.10286055194972175No Hit
TTGGACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC6940.10197889007586701No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA6920.10168500278458209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGT351.0187432E-746.00000440
GACCGTT351.0187432E-746.0000049
GGCGTAA351.0187432E-746.00000434
GTCTATG351.0187432E-746.00000410
TATTGGA351.0187432E-746.00000444
AACGCAC351.0187432E-746.00000436
CTCATAA750.046.00000437
TCGTACC351.0187432E-746.00000438
ACGTCTA351.0187432E-746.00000423
CATCGAA351.0187432E-746.00000425
GCAATCT351.0187432E-746.00000424
GCGTAAC351.0187432E-746.00000435
TTCTATC206.30963E-446.044
GTTGACG206.30963E-446.028
GCCTTAT206.30963E-446.020
TCGCAAT206.30963E-446.043
TACCCAC301.860275E-646.029
CGAACAC206.30963E-446.032
CGAACAA206.30963E-446.025
CGTATAT551.8189894E-1246.040
Produced by FastQC (version 0.11.5)